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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 271 |
| Acc36I | ACCTGC | 2 cut(s) | 124, 271 |
| Acc65I | GGTACC | 2 cut(s) | 109, 136 |
| AccB1I | GGYRCC | 3 cut(s) | 109, 136, 383 |
| AccBSI | CCGCTC | 1 cut(s) | 418 |
| AccI | GTMKAC | 1 cut(s) | 88 |
| AciI | CCGC | 7 cut(s) | 140, 181, 203, 226, 274, 289, 416 |
| AcyI | GRCGYC | 1 cut(s) | 384 |
| AfaI | GTAC | 2 cut(s) | 111, 138 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 98, 187, 190, 334, 376 |
| AgsI | TTSAA | 1 cut(s) | 251 |
| AjnI | CCWGG | 4 cut(s) | 236, 269, 297, 333 |
| AluBI | AGCT | 1 cut(s) | 280 |
| AluI | AGCT | 1 cut(s) | 280 |
| Alw21I | GWGCWC | 1 cut(s) | 77 |
| AoxI | GGCC | 2 cut(s) | 163, 410 |
| ApeKI | GCWGC | 1 cut(s) | 233 |
| Asp718I | GGTACC | 2 cut(s) | 109, 136 |
| AspLEI | GCGC | 2 cut(s) | 31, 386 |
| AspS9I | GGNCC | 2 cut(s) | 163, 410 |
| AsuC2I | CCSGG | 2 cut(s) | 230, 371 |
| BanI | GGYRCC | 3 cut(s) | 109, 136, 383 |
| Bbv12I | GWGCWC | 1 cut(s) | 77 |
| BbvI | GCAGC | 1 cut(s) | 245 |
| BccI | CCATC | 1 cut(s) | 154 |
| BciT130I | CCWGG | 4 cut(s) | 238, 271, 299, 335 |
| BcnI | CCSGG | 2 cut(s) | 230, 371 |
| BfaI | CTAG | 1 cut(s) | 243 |
| BfoI | RGCGCY | 2 cut(s) | 32, 387 |
| BfuAI | ACCTGC | 2 cut(s) | 124, 271 |
| BisI | GCNGC | 1 cut(s) | 234 |
| BlsI | GCNGC | 1 cut(s) | 235 |
| Bme1390I | CCNGG | 6 cut(s) | 230, 238, 271, 299, 335, 371 |
| BmgT120I | GGNCC | 2 cut(s) | 163, 410 |
| BmiI | GGNNCC | 4 cut(s) | 111, 138, 293, 385 |
| BmrFI | CCNGG | 6 cut(s) | 230, 238, 271, 299, 335, 371 |
| BmsI | GCATC | 2 cut(s) | 120, 333 |
| BpmI | CTGGAG | 1 cut(s) | 281 |
| BpuMI | CCSGG | 2 cut(s) | 230, 371 |
| BsaHI | GRCGYC | 1 cut(s) | 384 |
| BsaJI | CCNNGG | 3 cut(s) | 237, 269, 425 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 98, 187, 190, 334, 376 |
| Bse1I | ACTGG | 1 cut(s) | 119 |
| BseBI | CCWGG | 4 cut(s) | 238, 271, 299, 335 |
| BseDI | CCNNGG | 3 cut(s) | 237, 269, 425 |
| BseGI | GGATG | 2 cut(s) | 348, 420 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 98, 187, 190, 334, 376 |
| BseNI | ACTGG | 1 cut(s) | 119 |
| BseRI | GAGGAG | 2 cut(s) | 85, 162 |
| BseXI | GCAGC | 1 cut(s) | 245 |
| BseYI | CCCAGC | 1 cut(s) | 388 |
| BsgI | GTGCAG | 1 cut(s) | 249 |
| BshFI | GGCC | 2 cut(s) | 165, 412 |
| BshNI | GGYRCC | 3 cut(s) | 109, 136, 383 |
| BsiHKAI | GWGCWC | 1 cut(s) | 77 |
| BsiSI | CCGG | 2 cut(s) | 230, 370 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 98, 187, 190, 334, 376 |
| BsnI | GGCC | 2 cut(s) | 165, 412 |
| Bsp1286I | GDGCHC | 1 cut(s) | 77 |
| Bsp19I | CCATGG | 1 cut(s) | 425 |
| BspACI | CCGC | 7 cut(s) | 140, 181, 203, 226, 274, 289, 416 |
| BspANI | GGCC | 2 cut(s) | 165, 412 |
| BspLI | GGNNCC | 4 cut(s) | 111, 138, 293, 385 |
| BspMI | ACCTGC | 2 cut(s) | 124, 271 |
| BspT107I | GGYRCC | 3 cut(s) | 109, 136, 383 |
| BsrBI | CCGCTC | 1 cut(s) | 418 |
| BsrI | ACTGG | 1 cut(s) | 119 |
| BssECI | CCNNGG | 3 cut(s) | 237, 269, 425 |
| BssNI | GRCGYC | 1 cut(s) | 384 |
| BssT1I | CCWWGG | 1 cut(s) | 425 |
| Bst2UI | CCWGG | 4 cut(s) | 238, 271, 299, 335 |
| BstACI | GRCGYC | 1 cut(s) | 384 |
| BstC8I | GCNNGC | 3 cut(s) | 167, 313, 382 |
| BstDSI | CCRYGG | 1 cut(s) | 425 |
| BstF5I | GGATG | 2 cut(s) | 348, 420 |
| BstH2I | RGCGCY | 2 cut(s) | 32, 387 |
| BstHHI | GCGC | 2 cut(s) | 31, 386 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 35, 81, 139, 175, 239, 286, 308 |
| BstNI | CCWGG | 4 cut(s) | 238, 271, 299, 335 |
| BstSCI | CCNGG | 6 cut(s) | 228, 236, 269, 297, 333, 369 |
| BstV1I | GCAGC | 1 cut(s) | 245 |
| BsuRI | GGCC | 2 cut(s) | 165, 412 |
| BtgI | CCRYGG | 1 cut(s) | 425 |
| BtsCI | GGATG | 2 cut(s) | 348, 420 |
| BveI | ACCTGC | 2 cut(s) | 124, 271 |
| Cac8I | GCNNGC | 3 cut(s) | 167, 313, 382 |
| CfoI | GCGC | 2 cut(s) | 31, 386 |
| Cfr13I | GGNCC | 2 cut(s) | 163, 410 |
| CseI | GACGC | 1 cut(s) | 90 |
| Csp6I | GTAC | 2 cut(s) | 110, 137 |
| CviAII | CATG | 1 cut(s) | 426 |
| CviJI | RGCY | 6 cut(s) | 165, 236, 242, 280, 364, 412 |
| CviKI_1 | RGCY | 6 cut(s) | 165, 236, 242, 280, 364, 412 |
| CviQI | GTAC | 2 cut(s) | 110, 137 |
| DinI | GGCGCC | 1 cut(s) | 385 |
| EciI | GGCGGA | 1 cut(s) | 218 |
| Eco130I | CCWWGG | 1 cut(s) | 425 |
| EcoRII | CCWGG | 4 cut(s) | 236, 269, 297, 333 |
| EcoT14I | CCWWGG | 1 cut(s) | 425 |
| EgeI | GGCGCC | 1 cut(s) | 385 |
| EheI | GGCGCC | 1 cut(s) | 385 |
| ErhI | CCWWGG | 1 cut(s) | 425 |
| FaeI | CATG | 1 cut(s) | 429 |
| FaiI | YATR | 3 cut(s) | 116, 125, 427 |
| FatI | CATG | 1 cut(s) | 425 |
| FauI | CCCGC | 2 cut(s) | 188, 267 |
| FblI | GTMKAC | 1 cut(s) | 88 |
| Fnu4HI | GCNGC | 1 cut(s) | 234 |
| FokI | GGATG | 2 cut(s) | 355, 407 |
| Fsp4HI | GCNGC | 1 cut(s) | 234 |
| FspBI | CTAG | 1 cut(s) | 243 |
| GlaI | GCGC | 2 cut(s) | 30, 385 |
| GluI | GCNGC | 1 cut(s) | 234 |
| GsaI | CCCAGC | 1 cut(s) | 392 |
| GsuI | CTGGAG | 1 cut(s) | 281 |
| HaeII | RGCGCY | 2 cut(s) | 32, 387 |
| HaeIII | GGCC | 2 cut(s) | 165, 412 |
| HapII | CCGG | 2 cut(s) | 230, 370 |
| HgaI | GACGC | 1 cut(s) | 90 |
| HhaI | GCGC | 2 cut(s) | 31, 386 |
| Hin1I | GRCGYC | 1 cut(s) | 384 |
| Hin1II | CATG | 1 cut(s) | 429 |
| Hin6I | GCGC | 2 cut(s) | 29, 384 |
| HinP1I | GCGC | 2 cut(s) | 29, 384 |
| HincII | GTYRAC | 1 cut(s) | 89 |
| HindII | GTYRAC | 1 cut(s) | 89 |
| HinfI | GANTC | 2 cut(s) | 206, 304 |
| HpaII | CCGG | 2 cut(s) | 230, 370 |
| Hpy166II | GTNNAC | 1 cut(s) | 89 |
| Hpy8I | GTNNAC | 1 cut(s) | 89 |
| HpyAV | CCTTC | 1 cut(s) | 245 |
| HpyCH4V | TGCA | 3 cut(s) | 133, 266, 346 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 35, 81, 139, 175, 239, 286, 308 |
| Hsp92I | GRCGYC | 1 cut(s) | 384 |
| Hsp92II | CATG | 1 cut(s) | 429 |
| HspAI | GCGC | 2 cut(s) | 29, 384 |
| KasI | GGCGCC | 1 cut(s) | 383 |
| KpnI | GGTACC | 2 cut(s) | 113, 140 |
| LmnI | GCTCC | 4 cut(s) | 61, 72, 193, 277 |
| Lsp1109I | GCAGC | 1 cut(s) | 245 |
| LweI | GCATC | 2 cut(s) | 120, 333 |
| MaeI | CTAG | 1 cut(s) | 243 |
| MbiI | CCGCTC | 1 cut(s) | 418 |
| MhlI | GDGCHC | 1 cut(s) | 77 |
| MluCI | AATT | 3 cut(s) | 17, 246, 350 |
| Mly113I | GGCGCC | 1 cut(s) | 384 |
| MlyI | GAGTC | 1 cut(s) | 313 |
| MnlI | CCTC | 7 cut(s) | 51, 63, 180, 183, 193, 305, 370 |
| MseI | TTAA | 2 cut(s) | 20, 353 |
| MslI | CAYNNNNRTG | 1 cut(s) | 128 |
| MspA1I | CMGCKG | 2 cut(s) | 142, 289 |
| MspI | CCGG | 2 cut(s) | 230, 370 |
| MspR9I | CCNGG | 6 cut(s) | 230, 238, 271, 299, 335, 371 |
| MvaI | CCWGG | 4 cut(s) | 238, 271, 299, 335 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 35, 81, 139, 175, 239, 286, 308 |
| NarI | GGCGCC | 1 cut(s) | 384 |
| NciI | CCSGG | 2 cut(s) | 230, 371 |
| NcoI | CCATGG | 1 cut(s) | 425 |
| NlaIII | CATG | 1 cut(s) | 429 |
| NlaIV | GGNNCC | 4 cut(s) | 111, 138, 293, 385 |
| PaqCI | CACCTGC | 1 cut(s) | 271 |
| PfeI | GAWTC | 1 cut(s) | 206 |
| PflFI | GACNNNGTC | 1 cut(s) | 84 |
| PfoI | TCCNGGA | 1 cut(s) | 333 |
| PkrI | GCNGC | 1 cut(s) | 235 |
| PleI | GAGTC | 1 cut(s) | 312 |
| PluTI | GGCGCC | 1 cut(s) | 387 |
| PpsI | GAGTC | 1 cut(s) | 312 |
| Psp6I | CCWGG | 4 cut(s) | 236, 269, 297, 333 |
| PspFI | CCCAGC | 1 cut(s) | 388 |
| PspGI | CCWGG | 4 cut(s) | 236, 269, 297, 333 |
| PspN4I | GGNNCC | 4 cut(s) | 111, 138, 293, 385 |
| PspPI | GGNCC | 2 cut(s) | 163, 410 |
| PsyI | GACNNNGTC | 1 cut(s) | 84 |
| RsaI | GTAC | 2 cut(s) | 111, 138 |
| RsaNI | GTAC | 2 cut(s) | 110, 137 |
| RseI | CAYNNNNRTG | 1 cut(s) | 128 |
| SalI | GTCGAC | 1 cut(s) | 87 |
| SaqAI | TTAA | 2 cut(s) | 20, 353 |
| SatI | GCNGC | 1 cut(s) | 234 |
| Sau96I | GGNCC | 2 cut(s) | 163, 410 |
| SchI | GAGTC | 1 cut(s) | 313 |
| ScrFI | CCNGG | 6 cut(s) | 230, 238, 271, 299, 335, 371 |
| SduI | GDGCHC | 1 cut(s) | 77 |
| SetI | ASST | 5 cut(s) | 138, 256, 265, 282, 297 |
| SfaNI | GCATC | 2 cut(s) | 120, 333 |
| SfoI | GGCGCC | 1 cut(s) | 385 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 128 |
| Sse9I | AATT | 3 cut(s) | 17, 246, 350 |
| SsiI | CCGC | 7 cut(s) | 140, 181, 203, 226, 274, 289, 416 |
| SspDI | GGCGCC | 1 cut(s) | 383 |
| SspMI | CTAG | 1 cut(s) | 243 |
| StyD4I | CCNGG | 6 cut(s) | 228, 236, 269, 297, 333, 369 |
| StyI | CCWWGG | 1 cut(s) | 425 |
| TaqI | TCGA | 3 cut(s) | 12, 68, 88 |
| TasI | AATT | 3 cut(s) | 17, 246, 350 |
| TfiI | GAWTC | 1 cut(s) | 206 |
| Tru1I | TTAA | 2 cut(s) | 20, 353 |
| Tru9I | TTAA | 2 cut(s) | 20, 353 |
| TseI | GCWGC | 1 cut(s) | 233 |
| TspDTI | ATGAA | 1 cut(s) | 30 |
| Tth111I | GACNNNGTC | 1 cut(s) | 84 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 277, 331 |
| XmiI | GTMKAC | 1 cut(s) | 88 |
| XspI | CTAG | 1 cut(s) | 243 |