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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 298 |
| AciI | CCGC | 7 cut(s) | 23, 44, 62, 312, 331, 351, 472 |
| AclWI | GGATC | 2 cut(s) | 34, 473 |
| AcoI | YGGCCR | 1 cut(s) | 201 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 142, 343, 376, 418, 460 |
| AgsI | TTSAA | 1 cut(s) | 232 |
| AjnI | CCWGG | 6 cut(s) | 73, 91, 96, 339, 375, 458 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 224, 256 |
| AluBI | AGCT | 3 cut(s) | 121, 254, 322 |
| AluI | AGCT | 3 cut(s) | 121, 254, 322 |
| Alw21I | GWGCWC | 1 cut(s) | 287 |
| AlwI | GGATC | 2 cut(s) | 34, 473 |
| AoxI | GGCC | 7 cut(s) | 94, 144, 186, 201, 347, 462, 487 |
| ApaI | GGGCCC | 1 cut(s) | 351 |
| ApeKI | GCWGC | 3 cut(s) | 78, 328, 388 |
| AspLEI | GCGC | 3 cut(s) | 171, 265, 428 |
| AspS9I | GGNCC | 5 cut(s) | 100, 187, 347, 348, 462 |
| AsuHPI | GGTGA | 1 cut(s) | 461 |
| AvaII | GGWCC | 1 cut(s) | 100 |
| BaeGI | GKGCMC | 1 cut(s) | 351 |
| BalI | TGGCCA | 1 cut(s) | 203 |
| BanII | GRGCYC | 1 cut(s) | 351 |
| Bbv12I | GWGCWC | 1 cut(s) | 287 |
| BbvI | GCAGC | 3 cut(s) | 90, 340, 400 |
| BceAI | ACGGC | 1 cut(s) | 474 |
| BciT130I | CCWGG | 6 cut(s) | 75, 93, 98, 341, 377, 460 |
| BfaI | CTAG | 2 cut(s) | 12, 323 |
| BfmI | CTRYAG | 2 cut(s) | 57, 483 |
| BfoI | RGCGCY | 1 cut(s) | 429 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 93 |
| BisI | GCNGC | 4 cut(s) | 23, 79, 329, 389 |
| BlsI | GCNGC | 4 cut(s) | 24, 80, 330, 390 |
| Bme1390I | CCNGG | 6 cut(s) | 75, 93, 98, 341, 377, 460 |
| Bme18I | GGWCC | 1 cut(s) | 100 |
| BmgT120I | GGNCC | 5 cut(s) | 100, 187, 347, 348, 462 |
| BmiI | GGNNCC | 3 cut(s) | 335, 348, 349 |
| BmrFI | CCNGG | 6 cut(s) | 75, 93, 98, 341, 377, 460 |
| BmsI | GCATC | 4 cut(s) | 349, 375, 502, 511 |
| BpmI | CTGGAG | 2 cut(s) | 323, 398 |
| BsaJI | CCNNGG | 2 cut(s) | 73, 459 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 142, 343, 376, 418, 460 |
| BseBI | CCWGG | 6 cut(s) | 75, 93, 98, 341, 377, 460 |
| BseDI | CCNNGG | 2 cut(s) | 73, 459 |
| BseGI | GGATG | 1 cut(s) | 390 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 142, 343, 376, 418, 460 |
| BseMII | CTCAG | 1 cut(s) | 50 |
| BseRI | GAGGAG | 1 cut(s) | 24 |
| BseSI | GKGCMC | 1 cut(s) | 351 |
| BseXI | GCAGC | 3 cut(s) | 90, 340, 400 |
| BseYI | CCCAGC | 1 cut(s) | 259 |
| BsgI | GTGCAG | 1 cut(s) | 347 |
| BshFI | GGCC | 7 cut(s) | 96, 146, 188, 203, 349, 464, 489 |
| BsiHKAI | GWGCWC | 1 cut(s) | 287 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 142, 343, 376, 418, 460 |
| BsnI | GGCC | 7 cut(s) | 96, 146, 188, 203, 349, 464, 489 |
| Bsp120I | GGGCCC | 1 cut(s) | 347 |
| Bsp1286I | GDGCHC | 2 cut(s) | 287, 351 |
| Bsp143I | GATC | 2 cut(s) | 39, 478 |
| BspACI | CCGC | 7 cut(s) | 23, 44, 62, 312, 331, 351, 472 |
| BspANI | GGCC | 7 cut(s) | 96, 146, 188, 203, 349, 464, 489 |
| BspCNI | CTCAG | 1 cut(s) | 49 |
| BspLI | GGNNCC | 3 cut(s) | 335, 348, 349 |
| BspMAI | CTGCAG | 1 cut(s) | 487 |
| BspPI | GGATC | 2 cut(s) | 34, 473 |
| BssECI | CCNNGG | 2 cut(s) | 73, 459 |
| BssMI | GATC | 2 cut(s) | 39, 478 |
| Bst2UI | CCWGG | 6 cut(s) | 75, 93, 98, 341, 377, 460 |
| BstC8I | GCNNGC | 6 cut(s) | 27, 70, 351, 355, 424, 487 |
| BstDEI | CTNAG | 1 cut(s) | 36 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 140, 341 |
| BstF5I | GGATG | 1 cut(s) | 390 |
| BstH2I | RGCGCY | 1 cut(s) | 429 |
| BstHHI | GCGC | 3 cut(s) | 171, 265, 428 |
| BstKTI | GATC | 2 cut(s) | 42, 481 |
| BstMBI | GATC | 2 cut(s) | 39, 478 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 78, 84, 93, 245, 260, 328, 359, 397, 521 |
| BstNI | CCWGG | 6 cut(s) | 75, 93, 98, 341, 377, 460 |
| BstNSI | RCATGY | 2 cut(s) | 29, 507 |
| BstSCI | CCNGG | 6 cut(s) | 73, 91, 96, 339, 375, 458 |
| BstSFI | CTRYAG | 2 cut(s) | 57, 483 |
| BstSLI | GKGCMC | 1 cut(s) | 351 |
| BstV1I | GCAGC | 3 cut(s) | 90, 340, 400 |
| BstX2I | RGATCY | 2 cut(s) | 39, 478 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 316 |
| BstYI | RGATCY | 2 cut(s) | 39, 478 |
| BsuRI | GGCC | 7 cut(s) | 96, 146, 188, 203, 349, 464, 489 |
| BtgZI | GCGATG | 1 cut(s) | 372 |
| BtsCI | GGATG | 1 cut(s) | 390 |
| Cac8I | GCNNGC | 6 cut(s) | 27, 70, 351, 355, 424, 487 |
| CfoI | GCGC | 3 cut(s) | 171, 265, 428 |
| Cfr13I | GGNCC | 5 cut(s) | 100, 187, 347, 348, 462 |
| CseI | GACGC | 1 cut(s) | 439 |
| CviAII | CATG | 4 cut(s) | 26, 112, 504, 533 |
| DdeI | CTNAG | 1 cut(s) | 36 |
| DpnI | GATC | 2 cut(s) | 41, 480 |
| DpnII | GATC | 2 cut(s) | 39, 478 |
| EaeI | YGGCCR | 1 cut(s) | 201 |
| Eco24I | GRGCYC | 1 cut(s) | 351 |
| Eco47I | GGWCC | 1 cut(s) | 100 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 140, 341 |
| EcoO109I | RGGNCCY | 2 cut(s) | 100, 347 |
| EcoRII | CCWGG | 6 cut(s) | 73, 91, 96, 339, 375, 458 |
| EcoT38I | GRGCYC | 1 cut(s) | 351 |
| FaeI | CATG | 4 cut(s) | 29, 115, 507, 536 |
| FaiI | YATR | 5 cut(s) | 27, 113, 117, 505, 534 |
| FatI | CATG | 4 cut(s) | 25, 111, 503, 532 |
| FauI | CCCGC | 2 cut(s) | 51, 358 |
| FblI | GTMKAC | 1 cut(s) | 298 |
| Fnu4HI | GCNGC | 4 cut(s) | 23, 79, 329, 389 |
| FokI | GGATG | 1 cut(s) | 397 |
| FriOI | GRGCYC | 1 cut(s) | 351 |
| Fsp4HI | GCNGC | 4 cut(s) | 23, 79, 329, 389 |
| FspBI | CTAG | 2 cut(s) | 12, 323 |
| GlaI | GCGC | 3 cut(s) | 170, 264, 427 |
| GluI | GCNGC | 4 cut(s) | 23, 79, 329, 389 |
| GsaI | CCCAGC | 1 cut(s) | 263 |
| GsuI | CTGGAG | 2 cut(s) | 323, 398 |
| HaeII | RGCGCY | 1 cut(s) | 429 |
| HaeIII | GGCC | 7 cut(s) | 96, 146, 188, 203, 349, 464, 489 |
| HgaI | GACGC | 1 cut(s) | 439 |
| HhaI | GCGC | 3 cut(s) | 171, 265, 428 |
| Hin1II | CATG | 4 cut(s) | 29, 115, 507, 536 |
| Hin6I | GCGC | 3 cut(s) | 169, 263, 426 |
| HinP1I | GCGC | 3 cut(s) | 169, 263, 426 |
| HincII | GTYRAC | 2 cut(s) | 7, 299 |
| HindII | GTYRAC | 2 cut(s) | 7, 299 |
| HinfI | GANTC | 2 cut(s) | 31, 219 |
| HphI | GGTGA | 1 cut(s) | 461 |
| Hpy166II | GTNNAC | 2 cut(s) | 7, 299 |
| Hpy188I | TCNGA | 3 cut(s) | 39, 413, 495 |
| Hpy188III | TCNNGA | 1 cut(s) | 216 |
| Hpy8I | GTNNAC | 2 cut(s) | 7, 299 |
| Hpy99I | CGWCG | 1 cut(s) | 110 |
| HpyAV | CCTTC | 1 cut(s) | 229 |
| HpyCH4IV | ACGT | 1 cut(s) | 292 |
| HpyCH4V | TGCA | 5 cut(s) | 328, 362, 388, 485, 507 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 78, 84, 93, 245, 260, 328, 359, 397, 521 |
| HpyF3I | CTNAG | 1 cut(s) | 36 |
| HpySE526I | ACGT | 1 cut(s) | 292 |
| Hsp92II | CATG | 4 cut(s) | 29, 115, 507, 536 |
| HspAI | GCGC | 3 cut(s) | 169, 263, 426 |
| Kzo9I | GATC | 2 cut(s) | 39, 478 |
| LmnI | GCTCC | 1 cut(s) | 319 |
| Lsp1109I | GCAGC | 3 cut(s) | 90, 340, 400 |
| LweI | GCATC | 4 cut(s) | 349, 375, 502, 511 |
| MaeI | CTAG | 2 cut(s) | 12, 323 |
| MaeII | ACGT | 1 cut(s) | 292 |
| MalI | GATC | 2 cut(s) | 41, 480 |
| MboI | GATC | 2 cut(s) | 39, 478 |
| MboII | GAAGA | 1 cut(s) | 244 |
| MflI | RGATCY | 2 cut(s) | 39, 478 |
| MhlI | GDGCHC | 2 cut(s) | 287, 351 |
| MlsI | TGGCCA | 1 cut(s) | 203 |
| MluCI | AATT | 2 cut(s) | 227, 392 |
| MluNI | TGGCCA | 1 cut(s) | 203 |
| MlyI | GAGTC | 1 cut(s) | 25 |
| Mox20I | TGGCCA | 1 cut(s) | 203 |
| MscI | TGGCCA | 1 cut(s) | 203 |
| MslI | CAYNNNNRTG | 1 cut(s) | 209 |
| Msp20I | TGGCCA | 1 cut(s) | 203 |
| MspA1I | CMGCKG | 4 cut(s) | 62, 312, 331, 353 |
| MspR9I | CCNGG | 6 cut(s) | 75, 93, 98, 341, 377, 460 |
| MvaI | CCWGG | 6 cut(s) | 75, 93, 98, 341, 377, 460 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 78, 84, 93, 245, 260, 328, 359, 397, 521 |
| NdeII | GATC | 2 cut(s) | 39, 478 |
| NlaIII | CATG | 4 cut(s) | 29, 115, 507, 536 |
| NlaIV | GGNNCC | 3 cut(s) | 335, 348, 349 |
| NspI | RCATGY | 2 cut(s) | 29, 507 |
| PaeI | GCATGC | 1 cut(s) | 29 |
| PfeI | GAWTC | 1 cut(s) | 219 |
| PflFI | GACNNNGTC | 2 cut(s) | 104, 294 |
| PfoI | TCCNGGA | 2 cut(s) | 339, 375 |
| PkrI | GCNGC | 4 cut(s) | 24, 80, 330, 390 |
| PleI | GAGTC | 1 cut(s) | 25 |
| PpsI | GAGTC | 1 cut(s) | 25 |
| PpuMI | RGGWCCY | 1 cut(s) | 100 |
| Psp5II | RGGWCCY | 1 cut(s) | 100 |
| Psp6I | CCWGG | 6 cut(s) | 73, 91, 96, 339, 375, 458 |
| PspFI | CCCAGC | 1 cut(s) | 259 |
| PspGI | CCWGG | 6 cut(s) | 73, 91, 96, 339, 375, 458 |
| PspN4I | GGNNCC | 3 cut(s) | 335, 348, 349 |
| PspOMI | GGGCCC | 1 cut(s) | 347 |
| PspPI | GGNCC | 5 cut(s) | 100, 187, 347, 348, 462 |
| PspPPI | RGGWCCY | 1 cut(s) | 100 |
| PstI | CTGCAG | 1 cut(s) | 487 |
| PsuI | RGATCY | 2 cut(s) | 39, 478 |
| PsyI | GACNNNGTC | 2 cut(s) | 104, 294 |
| RseI | CAYNNNNRTG | 1 cut(s) | 209 |
| SalI | GTCGAC | 1 cut(s) | 297 |
| SatI | GCNGC | 4 cut(s) | 23, 79, 329, 389 |
| Sau3AI | GATC | 2 cut(s) | 39, 478 |
| Sau96I | GGNCC | 5 cut(s) | 100, 187, 347, 348, 462 |
| SbfI | CCTGCAGG | 1 cut(s) | 487 |
| SchI | GAGTC | 1 cut(s) | 25 |
| ScrFI | CCNGG | 6 cut(s) | 75, 93, 98, 341, 377, 460 |
| SdaI | CCTGCAGG | 1 cut(s) | 487 |
| SduI | GDGCHC | 2 cut(s) | 287, 351 |
| SetI | ASST | 8 cut(s) | 105, 123, 138, 183, 256, 295, 324, 339 |
| SfaNI | GCATC | 4 cut(s) | 349, 375, 502, 511 |
| SfcI | CTRYAG | 2 cut(s) | 57, 483 |
| SinI | GGWCC | 1 cut(s) | 100 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 209 |
| SphI | GCATGC | 1 cut(s) | 29 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 487 |
| Sse9I | AATT | 2 cut(s) | 227, 392 |
| SsiI | CCGC | 7 cut(s) | 23, 44, 62, 312, 331, 351, 472 |
| SspMI | CTAG | 2 cut(s) | 12, 323 |
| StyD4I | CCNGG | 6 cut(s) | 73, 91, 96, 339, 375, 458 |
| TaiI | ACGT | 1 cut(s) | 295 |
| TaqI | TCGA | 3 cut(s) | 183, 222, 298 |
| TasI | AATT | 2 cut(s) | 227, 392 |
| TauI | GCSGC | 1 cut(s) | 25 |
| TfiI | GAWTC | 1 cut(s) | 219 |
| TseI | GCWGC | 3 cut(s) | 78, 328, 388 |
| TspDTI | ATGAA | 1 cut(s) | 240 |
| Tth111I | GACNNNGTC | 2 cut(s) | 104, 294 |
| VpaK11BI | GGWCC | 1 cut(s) | 100 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 140, 341 |
| XceI | RCATGY | 2 cut(s) | 29, 507 |
| XmiI | GTMKAC | 1 cut(s) | 298 |
| XspI | CTAG | 2 cut(s) | 12, 323 |