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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 394 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 641 |
| Acc36I | ACCTGC | 3 cut(s) | 210, 241, 394 |
| Acc65I | GGTACC | 3 cut(s) | 226, 253, 506 |
| AccB1I | GGYRCC | 3 cut(s) | 226, 253, 506 |
| AccBSI | CCGCTC | 1 cut(s) | 351 |
| AccII | CGCG | 1 cut(s) | 187 |
| AciI | CCGC | 6 cut(s) | 257, 349, 397, 425, 497, 624 |
| AclWI | GGATC | 2 cut(s) | 562, 575 |
| AcoI | YGGCCR | 1 cut(s) | 108 |
| AcuI | CTGAAG | 1 cut(s) | 158 |
| AfaI | GTAC | 3 cut(s) | 228, 255, 508 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 215, 303, 396, 397, 424, 457 |
| AgeI | ACCGGT | 1 cut(s) | 434 |
| AjnI | CCWGG | 7 cut(s) | 194, 302, 420, 492, 542, 547, 563 |
| AluBI | AGCT | 2 cut(s) | 28, 136 |
| AluI | AGCT | 2 cut(s) | 28, 136 |
| Alw26I | GTCTC | 1 cut(s) | 494 |
| AlwI | GGATC | 2 cut(s) | 562, 575 |
| AoxI | GGCC | 6 cut(s) | 93, 108, 286, 405, 428, 545 |
| ApaI | GGGCCC | 2 cut(s) | 290, 432 |
| ApeKI | GCWGC | 1 cut(s) | 201 |
| AsiGI | ACCGGT | 1 cut(s) | 434 |
| Asp718I | GGTACC | 3 cut(s) | 226, 253, 506 |
| AspLEI | GCGC | 6 cut(s) | 66, 78, 148, 172, 201, 592 |
| AspS9I | GGNCC | 9 cut(s) | 7, 94, 233, 286, 287, 406, 428, 429, 551 |
| AsuC2I | CCSGG | 1 cut(s) | 231 |
| AsuHPI | GGTGA | 1 cut(s) | 449 |
| AvaII | GGWCC | 3 cut(s) | 7, 233, 551 |
| BaeGI | GKGCMC | 2 cut(s) | 290, 432 |
| BalI | TGGCCA | 1 cut(s) | 110 |
| BamHI | GGATCC | 1 cut(s) | 567 |
| BanI | GGYRCC | 3 cut(s) | 226, 253, 506 |
| BanII | GRGCYC | 2 cut(s) | 290, 432 |
| BbvI | GCAGC | 1 cut(s) | 213 |
| BccI | CCATC | 3 cut(s) | 277, 397, 587 |
| BciT130I | CCWGG | 7 cut(s) | 196, 304, 422, 494, 544, 549, 565 |
| BcnI | CCSGG | 1 cut(s) | 231 |
| BcoDI | GTCTC | 1 cut(s) | 494 |
| BfaI | CTAG | 2 cut(s) | 366, 476 |
| BfoI | RGCGCY | 1 cut(s) | 149 |
| BfuAI | ACCTGC | 3 cut(s) | 210, 241, 394 |
| BisI | GCNGC | 2 cut(s) | 202, 625 |
| BlsI | GCNGC | 2 cut(s) | 203, 626 |
| Bme1390I | CCNGG | 8 cut(s) | 196, 231, 304, 422, 494, 544, 549, 565 |
| Bme18I | GGWCC | 3 cut(s) | 7, 233, 551 |
| BmgT120I | GGNCC | 9 cut(s) | 7, 94, 233, 286, 287, 406, 428, 429, 551 |
| BmiI | GGNNCC | 8 cut(s) | 228, 255, 288, 416, 429, 430, 508, 569 |
| BmrFI | CCNGG | 8 cut(s) | 196, 231, 304, 422, 494, 544, 549, 565 |
| BmsI | GCATC | 2 cut(s) | 237, 456 |
| BpmI | CTGGAG | 1 cut(s) | 404 |
| BpuMI | CCSGG | 1 cut(s) | 231 |
| BsaJI | CCNNGG | 1 cut(s) | 302 |
| BsaWI | WCCGGW | 1 cut(s) | 434 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 215, 303, 396, 397, 424, 457 |
| Bse118I | RCCGGY | 1 cut(s) | 434 |
| Bse1I | ACTGG | 2 cut(s) | 39, 462 |
| BseBI | CCWGG | 7 cut(s) | 196, 304, 422, 494, 544, 549, 565 |
| BseDI | CCNNGG | 1 cut(s) | 302 |
| BseGI | GGATG | 2 cut(s) | 288, 579 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 215, 303, 396, 397, 424, 457 |
| BseNI | ACTGG | 2 cut(s) | 39, 462 |
| BseSI | GKGCMC | 2 cut(s) | 290, 432 |
| BseXI | GCAGC | 1 cut(s) | 213 |
| BseYI | CCCAGC | 2 cut(s) | 166, 586 |
| Bsh1236I | CGCG | 1 cut(s) | 187 |
| BshFI | GGCC | 6 cut(s) | 95, 110, 288, 407, 430, 547 |
| BshNI | GGYRCC | 3 cut(s) | 226, 253, 506 |
| BshTI | ACCGGT | 1 cut(s) | 434 |
| BsiSI | CCGG | 2 cut(s) | 231, 435 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 215, 303, 396, 397, 424, 457 |
| BsmAI | GTCTC | 1 cut(s) | 494 |
| BsnI | GGCC | 6 cut(s) | 95, 110, 288, 407, 430, 547 |
| Bsp120I | GGGCCC | 2 cut(s) | 286, 428 |
| Bsp1286I | GDGCHC | 2 cut(s) | 290, 432 |
| Bsp143I | GATC | 2 cut(s) | 357, 567 |
| Bsp68I | TCGCGA | 1 cut(s) | 187 |
| BspACI | CCGC | 6 cut(s) | 257, 349, 397, 425, 497, 624 |
| BspANI | GGCC | 6 cut(s) | 95, 110, 288, 407, 430, 547 |
| BspFNI | CGCG | 1 cut(s) | 187 |
| BspLI | GGNNCC | 8 cut(s) | 228, 255, 288, 416, 429, 430, 508, 569 |
| BspMI | ACCTGC | 3 cut(s) | 210, 241, 394 |
| BspPI | GGATC | 2 cut(s) | 562, 575 |
| BspT107I | GGYRCC | 3 cut(s) | 226, 253, 506 |
| BsrBI | CCGCTC | 1 cut(s) | 351 |
| BsrFI | RCCGGY | 1 cut(s) | 434 |
| BsrI | ACTGG | 2 cut(s) | 39, 462 |
| BssAI | RCCGGY | 1 cut(s) | 434 |
| BssECI | CCNNGG | 1 cut(s) | 302 |
| BssMI | GATC | 2 cut(s) | 357, 567 |
| Bst2UI | CCWGG | 7 cut(s) | 196, 304, 422, 494, 544, 549, 565 |
| BstC8I | GCNNGC | 5 cut(s) | 30, 68, 179, 206, 594 |
| BstF5I | GGATG | 2 cut(s) | 288, 579 |
| BstFNI | CGCG | 1 cut(s) | 187 |
| BstH2I | RGCGCY | 1 cut(s) | 149 |
| BstHHI | GCGC | 6 cut(s) | 66, 78, 148, 172, 201, 592 |
| BstKTI | GATC | 2 cut(s) | 360, 570 |
| BstMAI | GTCTC | 1 cut(s) | 494 |
| BstMBI | GATC | 2 cut(s) | 357, 567 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 152, 167, 178, 198, 205, 256, 362 |
| BstNI | CCWGG | 7 cut(s) | 196, 304, 422, 494, 544, 549, 565 |
| BstSCI | CCNGG | 8 cut(s) | 194, 229, 302, 420, 492, 542, 547, 563 |
| BstSLI | GKGCMC | 2 cut(s) | 290, 432 |
| BstUI | CGCG | 1 cut(s) | 187 |
| BstV1I | GCAGC | 1 cut(s) | 213 |
| BstX2I | RGATCY | 1 cut(s) | 567 |
| BstYI | RGATCY | 1 cut(s) | 567 |
| BsuRI | GGCC | 6 cut(s) | 95, 110, 288, 407, 430, 547 |
| BtsCI | GGATG | 2 cut(s) | 288, 579 |
| BtsIMutI | CAGTG | 1 cut(s) | 32 |
| BtuMI | TCGCGA | 1 cut(s) | 187 |
| BveI | ACCTGC | 3 cut(s) | 210, 241, 394 |
| Cac8I | GCNNGC | 5 cut(s) | 30, 68, 179, 206, 594 |
| CfoI | GCGC | 6 cut(s) | 66, 78, 148, 172, 201, 592 |
| Cfr10I | RCCGGY | 1 cut(s) | 434 |
| Cfr13I | GGNCC | 9 cut(s) | 7, 94, 233, 286, 287, 406, 428, 429, 551 |
| CseI | GACGC | 3 cut(s) | 449, 528, 610 |
| Csp6I | GTAC | 3 cut(s) | 227, 254, 507 |
| CspAI | ACCGGT | 1 cut(s) | 434 |
| CviAII | CATG | 4 cut(s) | 112, 320, 555, 628 |
| CviQI | GTAC | 3 cut(s) | 227, 254, 507 |
| DpnI | GATC | 2 cut(s) | 359, 569 |
| DpnII | GATC | 2 cut(s) | 357, 567 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 641 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 641 |
| EaeI | YGGCCR | 1 cut(s) | 108 |
| EciI | GGCGGA | 1 cut(s) | 512 |
| Eco24I | GRGCYC | 2 cut(s) | 290, 432 |
| Eco47I | GGWCC | 3 cut(s) | 7, 233, 551 |
| Eco57I | CTGAAG | 1 cut(s) | 158 |
| EcoO109I | RGGNCCY | 2 cut(s) | 7, 428 |
| EcoRII | CCWGG | 7 cut(s) | 194, 302, 420, 492, 542, 547, 563 |
| EcoT38I | GRGCYC | 2 cut(s) | 290, 432 |
| FaeI | CATG | 4 cut(s) | 115, 323, 558, 631 |
| FaiI | YATR | 9 cut(s) | 20, 24, 113, 242, 321, 556, 629, 648, 650 |
| FatI | CATG | 4 cut(s) | 111, 319, 554, 627 |
| FauI | CCCGC | 2 cut(s) | 390, 418 |
| FauNDI | CATATG | 1 cut(s) | 648 |
| Fnu4HI | GCNGC | 2 cut(s) | 202, 625 |
| FokI | GGATG | 2 cut(s) | 295, 566 |
| FriOI | GRGCYC | 2 cut(s) | 290, 432 |
| Fsp4HI | GCNGC | 2 cut(s) | 202, 625 |
| FspBI | CTAG | 2 cut(s) | 366, 476 |
| GlaI | GCGC | 6 cut(s) | 65, 77, 147, 171, 200, 591 |
| GluI | GCNGC | 2 cut(s) | 202, 625 |
| GsaI | CCCAGC | 2 cut(s) | 170, 590 |
| GsuI | CTGGAG | 1 cut(s) | 404 |
| HaeII | RGCGCY | 1 cut(s) | 149 |
| HaeIII | GGCC | 6 cut(s) | 95, 110, 288, 407, 430, 547 |
| HapII | CCGG | 2 cut(s) | 231, 435 |
| HgaI | GACGC | 3 cut(s) | 449, 528, 610 |
| HhaI | GCGC | 6 cut(s) | 66, 78, 148, 172, 201, 592 |
| Hin1II | CATG | 4 cut(s) | 115, 323, 558, 631 |
| Hin6I | GCGC | 6 cut(s) | 64, 76, 146, 170, 199, 590 |
| HinP1I | GCGC | 6 cut(s) | 64, 76, 146, 170, 199, 590 |
| HinfI | GANTC | 1 cut(s) | 40 |
| HpaII | CCGG | 2 cut(s) | 231, 435 |
| HphI | GGTGA | 1 cut(s) | 449 |
| Hpy188III | TCNNGA | 2 cut(s) | 123, 186 |
| HpyAV | CCTTC | 1 cut(s) | 136 |
| HpyCH4V | TGCA | 4 cut(s) | 4, 250, 469, 558 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 152, 167, 178, 198, 205, 256, 362 |
| Hsp92II | CATG | 4 cut(s) | 115, 323, 558, 631 |
| HspAI | GCGC | 6 cut(s) | 64, 76, 146, 170, 199, 590 |
| KpnI | GGTACC | 3 cut(s) | 230, 257, 510 |
| Kzo9I | GATC | 2 cut(s) | 357, 567 |
| LmnI | GCTCC | 1 cut(s) | 73 |
| Lsp1109I | GCAGC | 1 cut(s) | 213 |
| LweI | GCATC | 2 cut(s) | 237, 456 |
| MaeI | CTAG | 2 cut(s) | 366, 476 |
| MaeIII | GTNAC | 3 cut(s) | 330, 437, 612 |
| MalI | GATC | 2 cut(s) | 359, 569 |
| MbiI | CCGCTC | 1 cut(s) | 351 |
| MboI | GATC | 2 cut(s) | 357, 567 |
| MboII | GAAGA | 2 cut(s) | 35, 151 |
| MflI | RGATCY | 1 cut(s) | 567 |
| MhlI | GDGCHC | 2 cut(s) | 290, 432 |
| MlsI | TGGCCA | 1 cut(s) | 110 |
| MluCI | AATT | 1 cut(s) | 369 |
| MluNI | TGGCCA | 1 cut(s) | 110 |
| MmeI | TCCRAC | 1 cut(s) | 294 |
| MnlI | CCTC | 5 cut(s) | 85, 303, 316, 367, 428 |
| Mox20I | TGGCCA | 1 cut(s) | 110 |
| MscI | TGGCCA | 1 cut(s) | 110 |
| MslI | CAYNNNNRTG | 2 cut(s) | 116, 245 |
| Msp20I | TGGCCA | 1 cut(s) | 110 |
| MspA1I | CMGCKG | 2 cut(s) | 259, 624 |
| MspI | CCGG | 2 cut(s) | 231, 435 |
| MspR9I | CCNGG | 8 cut(s) | 196, 231, 304, 422, 494, 544, 549, 565 |
| MvaI | CCWGG | 7 cut(s) | 196, 304, 422, 494, 544, 549, 565 |
| MvnI | CGCG | 1 cut(s) | 187 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 152, 167, 178, 198, 205, 256, 362 |
| NciI | CCSGG | 1 cut(s) | 231 |
| NdeI | CATATG | 1 cut(s) | 648 |
| NdeII | GATC | 2 cut(s) | 357, 567 |
| NlaIII | CATG | 4 cut(s) | 115, 323, 558, 631 |
| NlaIV | GGNNCC | 8 cut(s) | 228, 255, 288, 416, 429, 430, 508, 569 |
| NmeAIII | GCCGAG | 1 cut(s) | 332 |
| NmuCI | GTSAC | 3 cut(s) | 330, 437, 612 |
| NruI | TCGCGA | 1 cut(s) | 187 |
| PaqCI | CACCTGC | 1 cut(s) | 394 |
| PfeI | GAWTC | 1 cut(s) | 40 |
| PflFI | GACNNNGTC | 1 cut(s) | 11 |
| PinAI | ACCGGT | 1 cut(s) | 434 |
| PkrI | GCNGC | 2 cut(s) | 203, 626 |
| PpuMI | RGGWCCY | 1 cut(s) | 7 |
| Psp5II | RGGWCCY | 1 cut(s) | 7 |
| Psp6I | CCWGG | 7 cut(s) | 194, 302, 420, 492, 542, 547, 563 |
| PspFI | CCCAGC | 2 cut(s) | 166, 586 |
| PspGI | CCWGG | 7 cut(s) | 194, 302, 420, 492, 542, 547, 563 |
| PspN4I | GGNNCC | 8 cut(s) | 228, 255, 288, 416, 429, 430, 508, 569 |
| PspOMI | GGGCCC | 2 cut(s) | 286, 428 |
| PspPI | GGNCC | 9 cut(s) | 7, 94, 233, 286, 287, 406, 428, 429, 551 |
| PspPPI | RGGWCCY | 1 cut(s) | 7 |
| PsuI | RGATCY | 1 cut(s) | 567 |
| PsyI | GACNNNGTC | 1 cut(s) | 11 |
| RruI | TCGCGA | 1 cut(s) | 187 |
| RsaI | GTAC | 3 cut(s) | 228, 255, 508 |
| RsaNI | GTAC | 3 cut(s) | 227, 254, 507 |
| RseI | CAYNNNNRTG | 2 cut(s) | 116, 245 |
| SatI | GCNGC | 2 cut(s) | 202, 625 |
| Sau3AI | GATC | 2 cut(s) | 357, 567 |
| Sau96I | GGNCC | 9 cut(s) | 7, 94, 233, 286, 287, 406, 428, 429, 551 |
| ScrFI | CCNGG | 8 cut(s) | 196, 231, 304, 422, 494, 544, 549, 565 |
| SduI | GDGCHC | 2 cut(s) | 290, 432 |
| SfaNI | GCATC | 2 cut(s) | 237, 456 |
| SgrAI | CRCCGGYG | 1 cut(s) | 434 |
| SinI | GGWCC | 3 cut(s) | 7, 233, 551 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 116, 245 |
| Sse9I | AATT | 1 cut(s) | 369 |
| SsiI | CCGC | 6 cut(s) | 257, 349, 397, 425, 497, 624 |
| SspMI | CTAG | 2 cut(s) | 366, 476 |
| StyD4I | CCNGG | 8 cut(s) | 194, 229, 302, 420, 492, 542, 547, 563 |
| TaqI | TCGA | 1 cut(s) | 90 |
| TasI | AATT | 1 cut(s) | 369 |
| TauI | GCSGC | 1 cut(s) | 627 |
| TfiI | GAWTC | 1 cut(s) | 40 |
| TscAI | CASTG | 1 cut(s) | 39 |
| TseFI | GTSAC | 3 cut(s) | 330, 437, 612 |
| TseI | GCWGC | 1 cut(s) | 201 |
| Tsp45I | GTSAC | 3 cut(s) | 330, 437, 612 |
| TspRI | CASTG | 1 cut(s) | 39 |
| Tth111I | GACNNNGTC | 1 cut(s) | 11 |
| VpaK11BI | GGWCC | 3 cut(s) | 7, 233, 551 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 310, 454 |
| XspI | CTAG | 2 cut(s) | 366, 476 |