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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 184 |
| Acc36I | ACCTGC | 2 cut(s) | 184, 331 |
| Acc65I | GGTACC | 2 cut(s) | 316, 343 |
| AccB1I | GGYRCC | 2 cut(s) | 316, 343 |
| AccBSI | CCGCTC | 1 cut(s) | 435 |
| AccI | GTMKAC | 1 cut(s) | 295 |
| AccII | CGCG | 1 cut(s) | 277 |
| AciI | CCGC | 6 cut(s) | 56, 59, 128, 277, 347, 433 |
| AclI | AACGTT | 1 cut(s) | 18 |
| AcoI | YGGCCR | 1 cut(s) | 198 |
| AcsI | RAATTY | 1 cut(s) | 215 |
| AfaI | GTAC | 3 cut(s) | 318, 324, 345 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 58, 139, 193, 305, 361, 397 |
| AgeI | ACCGGT | 1 cut(s) | 88 |
| AhlI | ACTAGT | 1 cut(s) | 325 |
| Alw21I | GWGCWC | 2 cut(s) | 31, 284 |
| Alw44I | GTGCAC | 1 cut(s) | 27 |
| AoxI | GGCC | 3 cut(s) | 198, 370, 447 |
| ApaI | GGGCCC | 1 cut(s) | 374 |
| ApaLI | GTGCAC | 1 cut(s) | 27 |
| ApeKI | GCWGC | 1 cut(s) | 75 |
| ApoI | RAATTY | 1 cut(s) | 215 |
| AsiGI | ACCGGT | 1 cut(s) | 88 |
| Asp718I | GGTACC | 2 cut(s) | 316, 343 |
| AspLEI | GCGC | 2 cut(s) | 168, 238 |
| AspS9I | GGNCC | 6 cut(s) | 91, 97, 130, 184, 370, 371 |
| AsuC2I | CCSGG | 2 cut(s) | 72, 95 |
| AsuHPI | GGTGA | 1 cut(s) | 376 |
| AvaII | GGWCC | 4 cut(s) | 91, 97, 130, 184 |
| BaeGI | GKGCMC | 2 cut(s) | 31, 374 |
| BalI | TGGCCA | 1 cut(s) | 200 |
| BanI | GGYRCC | 2 cut(s) | 316, 343 |
| BanII | GRGCYC | 1 cut(s) | 374 |
| Bbv12I | GWGCWC | 2 cut(s) | 31, 284 |
| BbvCI | CCTCAGC | 1 cut(s) | 180 |
| BbvI | GCAGC | 1 cut(s) | 87 |
| BccI | CCATC | 3 cut(s) | 52, 361, 391 |
| BcnI | CCSGG | 2 cut(s) | 72, 95 |
| BcuI | ACTAGT | 1 cut(s) | 325 |
| BfaI | CTAG | 2 cut(s) | 6, 326 |
| BfuAI | ACCTGC | 2 cut(s) | 184, 331 |
| BisI | GCNGC | 3 cut(s) | 57, 76, 433 |
| BlsI | GCNGC | 3 cut(s) | 58, 77, 434 |
| Bme1390I | CCNGG | 2 cut(s) | 72, 95 |
| Bme18I | GGWCC | 4 cut(s) | 91, 97, 130, 184 |
| BmgT120I | GGNCC | 6 cut(s) | 91, 97, 130, 184, 370, 371 |
| BmiI | GGNNCC | 4 cut(s) | 98, 318, 345, 372 |
| BmrFI | CCNGG | 2 cut(s) | 72, 95 |
| BmsI | GCATC | 1 cut(s) | 327 |
| Bpu10I | CCTNAGC | 1 cut(s) | 180 |
| BpuMI | CCSGG | 2 cut(s) | 72, 95 |
| BsaWI | WCCGGW | 1 cut(s) | 88 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 58, 139, 193, 305, 361, 397 |
| Bse118I | RCCGGY | 1 cut(s) | 88 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 58, 139, 193, 305, 361, 397 |
| BseMII | CTCAG | 1 cut(s) | 171 |
| BseRI | GAGGAG | 1 cut(s) | 369 |
| BseSI | GKGCMC | 2 cut(s) | 31, 374 |
| BseXI | GCAGC | 1 cut(s) | 87 |
| BseYI | CCCAGC | 1 cut(s) | 66 |
| Bsh1236I | CGCG | 1 cut(s) | 277 |
| BshFI | GGCC | 3 cut(s) | 200, 372, 449 |
| BshNI | GGYRCC | 2 cut(s) | 316, 343 |
| BshTI | ACCGGT | 1 cut(s) | 88 |
| BsiHKAI | GWGCWC | 2 cut(s) | 31, 284 |
| BsiSI | CCGG | 3 cut(s) | 71, 89, 94 |
| BslFI | GGGAC | 1 cut(s) | 110 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 58, 139, 193, 305, 361, 397 |
| BsmFI | GGGAC | 1 cut(s) | 110 |
| BsnI | GGCC | 3 cut(s) | 200, 372, 449 |
| Bsp120I | GGGCCC | 1 cut(s) | 370 |
| Bsp1286I | GDGCHC | 3 cut(s) | 31, 284, 374 |
| BspACI | CCGC | 6 cut(s) | 56, 59, 128, 277, 347, 433 |
| BspANI | GGCC | 3 cut(s) | 200, 372, 449 |
| BspCNI | CTCAG | 1 cut(s) | 172 |
| BspFNI | CGCG | 1 cut(s) | 277 |
| BspLI | GGNNCC | 4 cut(s) | 98, 318, 345, 372 |
| BspMI | ACCTGC | 2 cut(s) | 184, 331 |
| BspT107I | GGYRCC | 2 cut(s) | 316, 343 |
| BsrBI | CCGCTC | 1 cut(s) | 435 |
| BsrFI | RCCGGY | 1 cut(s) | 88 |
| BssAI | RCCGGY | 1 cut(s) | 88 |
| BstC8I | GCNNGC | 2 cut(s) | 269, 307 |
| BstDEI | CTNAG | 2 cut(s) | 180, 444 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 137 |
| BstFNI | CGCG | 1 cut(s) | 277 |
| BstHHI | GCGC | 2 cut(s) | 168, 238 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 81, 346, 446 |
| BstSCI | CCNGG | 2 cut(s) | 70, 93 |
| BstSLI | GKGCMC | 2 cut(s) | 31, 374 |
| BstUI | CGCG | 1 cut(s) | 277 |
| BstV1I | GCAGC | 1 cut(s) | 87 |
| BsuRI | GGCC | 3 cut(s) | 200, 372, 449 |
| BveI | ACCTGC | 2 cut(s) | 184, 331 |
| Cac8I | GCNNGC | 2 cut(s) | 269, 307 |
| CfoI | GCGC | 2 cut(s) | 168, 238 |
| Cfr10I | RCCGGY | 1 cut(s) | 88 |
| Cfr13I | GGNCC | 6 cut(s) | 91, 97, 130, 184, 370, 371 |
| CpoI | CGGWCCG | 1 cut(s) | 91 |
| Csp6I | GTAC | 3 cut(s) | 317, 323, 344 |
| CspAI | ACCGGT | 1 cut(s) | 88 |
| CspI | CGGWCCG | 1 cut(s) | 91 |
| CviAII | CATG | 2 cut(s) | 202, 387 |
| CviJI | RGCY | 8 cut(s) | 70, 122, 179, 200, 372, 421, 432, 449 |
| CviKI_1 | RGCY | 8 cut(s) | 70, 122, 179, 200, 372, 421, 432, 449 |
| CviQI | GTAC | 3 cut(s) | 317, 323, 344 |
| DdeI | CTNAG | 2 cut(s) | 180, 444 |
| EaeI | YGGCCR | 1 cut(s) | 198 |
| EciI | GGCGGA | 2 cut(s) | 74, 143 |
| Eco24I | GRGCYC | 1 cut(s) | 374 |
| Eco47I | GGWCC | 4 cut(s) | 91, 97, 130, 184 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 137 |
| EcoO109I | RGGNCCY | 1 cut(s) | 97 |
| EcoT38I | GRGCYC | 1 cut(s) | 374 |
| FaeI | CATG | 2 cut(s) | 205, 390 |
| FaiI | YATR | 9 cut(s) | 110, 114, 156, 170, 203, 291, 332, 388, 457 |
| FaqI | GGGAC | 1 cut(s) | 110 |
| FatI | CATG | 2 cut(s) | 201, 386 |
| FblI | GTMKAC | 1 cut(s) | 295 |
| Fnu4HI | GCNGC | 3 cut(s) | 57, 76, 433 |
| FriOI | GRGCYC | 1 cut(s) | 374 |
| Fsp4HI | GCNGC | 3 cut(s) | 57, 76, 433 |
| FspBI | CTAG | 2 cut(s) | 6, 326 |
| GlaI | GCGC | 2 cut(s) | 167, 237 |
| GluI | GCNGC | 3 cut(s) | 57, 76, 433 |
| GsaI | CCCAGC | 1 cut(s) | 70 |
| HaeIII | GGCC | 3 cut(s) | 200, 372, 449 |
| HapII | CCGG | 3 cut(s) | 71, 89, 94 |
| HhaI | GCGC | 2 cut(s) | 168, 238 |
| Hin1II | CATG | 2 cut(s) | 205, 390 |
| Hin6I | GCGC | 2 cut(s) | 166, 236 |
| HinP1I | GCGC | 2 cut(s) | 166, 236 |
| HincII | GTYRAC | 1 cut(s) | 296 |
| HindII | GTYRAC | 1 cut(s) | 296 |
| HinfI | GANTC | 1 cut(s) | 413 |
| HpaII | CCGG | 3 cut(s) | 71, 89, 94 |
| HphI | GGTGA | 1 cut(s) | 376 |
| Hpy166II | GTNNAC | 3 cut(s) | 16, 29, 296 |
| Hpy8I | GTNNAC | 3 cut(s) | 16, 29, 296 |
| HpyAV | CCTTC | 1 cut(s) | 169 |
| HpyCH4IV | ACGT | 1 cut(s) | 18 |
| HpyCH4V | TGCA | 3 cut(s) | 29, 305, 340 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 81, 346, 446 |
| HpyF3I | CTNAG | 2 cut(s) | 180, 444 |
| HpySE526I | ACGT | 1 cut(s) | 18 |
| Hsp92II | CATG | 2 cut(s) | 205, 390 |
| HspAI | GCGC | 2 cut(s) | 166, 236 |
| KpnI | GGTACC | 2 cut(s) | 320, 347 |
| LmnI | GCTCC | 1 cut(s) | 279 |
| Lsp1109I | GCAGC | 1 cut(s) | 87 |
| LweI | GCATC | 1 cut(s) | 327 |
| MaeI | CTAG | 2 cut(s) | 6, 326 |
| MaeII | ACGT | 1 cut(s) | 18 |
| MbiI | CCGCTC | 1 cut(s) | 435 |
| MboII | GAAGA | 1 cut(s) | 254 |
| MhlI | GDGCHC | 3 cut(s) | 31, 284, 374 |
| MlsI | TGGCCA | 1 cut(s) | 200 |
| MluCI | AATT | 2 cut(s) | 215, 224 |
| MluNI | TGGCCA | 1 cut(s) | 200 |
| MnlI | CCTC | 8 cut(s) | 110, 115, 143, 175, 355, 387, 390, 400 |
| Mox20I | TGGCCA | 1 cut(s) | 200 |
| MscI | TGGCCA | 1 cut(s) | 200 |
| MseI | TTAA | 3 cut(s) | 21, 36, 227 |
| MslI | CAYNNNNRTG | 2 cut(s) | 206, 335 |
| Msp20I | TGGCCA | 1 cut(s) | 200 |
| MspA1I | CMGCKG | 1 cut(s) | 349 |
| MspI | CCGG | 3 cut(s) | 71, 89, 94 |
| MspR9I | CCNGG | 2 cut(s) | 72, 95 |
| MvnI | CGCG | 1 cut(s) | 277 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 81, 346, 446 |
| NciI | CCSGG | 2 cut(s) | 72, 95 |
| NlaIII | CATG | 2 cut(s) | 205, 390 |
| NlaIV | GGNNCC | 4 cut(s) | 98, 318, 345, 372 |
| NmeAIII | GCCGAG | 1 cut(s) | 416 |
| PaqCI | CACCTGC | 1 cut(s) | 184 |
| PfeI | GAWTC | 1 cut(s) | 413 |
| PfoI | TCCNGGA | 1 cut(s) | 93 |
| PinAI | ACCGGT | 1 cut(s) | 88 |
| PkrI | GCNGC | 3 cut(s) | 58, 77, 434 |
| PpuMI | RGGWCCY | 1 cut(s) | 97 |
| Psp1406I | AACGTT | 1 cut(s) | 18 |
| Psp5II | RGGWCCY | 1 cut(s) | 97 |
| PspFI | CCCAGC | 1 cut(s) | 66 |
| PspN4I | GGNNCC | 4 cut(s) | 98, 318, 345, 372 |
| PspOMI | GGGCCC | 1 cut(s) | 370 |
| PspPI | GGNCC | 6 cut(s) | 91, 97, 130, 184, 370, 371 |
| PspPPI | RGGWCCY | 1 cut(s) | 97 |
| RsaI | GTAC | 3 cut(s) | 318, 324, 345 |
| RsaNI | GTAC | 3 cut(s) | 317, 323, 344 |
| RseI | CAYNNNNRTG | 2 cut(s) | 206, 335 |
| Rsr2I | CGGWCCG | 1 cut(s) | 91 |
| RsrII | CGGWCCG | 1 cut(s) | 91 |
| SalI | GTCGAC | 1 cut(s) | 294 |
| SaqAI | TTAA | 3 cut(s) | 21, 36, 227 |
| SatI | GCNGC | 3 cut(s) | 57, 76, 433 |
| Sau96I | GGNCC | 6 cut(s) | 91, 97, 130, 184, 370, 371 |
| ScrFI | CCNGG | 2 cut(s) | 72, 95 |
| SduI | GDGCHC | 3 cut(s) | 31, 284, 374 |
| SetI | ASST | 9 cut(s) | 21, 54, 102, 135, 186, 198, 345, 366, 379 |
| SfaNI | GCATC | 1 cut(s) | 327 |
| SinI | GGWCC | 4 cut(s) | 91, 97, 130, 184 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 206, 335 |
| SpeI | ACTAGT | 1 cut(s) | 325 |
| Sse9I | AATT | 2 cut(s) | 215, 224 |
| SsiI | CCGC | 6 cut(s) | 56, 59, 128, 277, 347, 433 |
| SspMI | CTAG | 2 cut(s) | 6, 326 |
| StyD4I | CCNGG | 2 cut(s) | 70, 93 |
| TaiI | ACGT | 1 cut(s) | 21 |
| TaqI | TCGA | 2 cut(s) | 219, 295 |
| TasI | AATT | 2 cut(s) | 215, 224 |
| TauI | GCSGC | 2 cut(s) | 59, 435 |
| TfiI | GAWTC | 1 cut(s) | 413 |
| Tru1I | TTAA | 3 cut(s) | 21, 36, 227 |
| Tru9I | TTAA | 3 cut(s) | 21, 36, 227 |
| TseI | GCWGC | 1 cut(s) | 75 |
| TspDTI | ATGAA | 1 cut(s) | 237 |
| VneI | GTGCAC | 1 cut(s) | 27 |
| VpaK11BI | GGWCC | 4 cut(s) | 91, 97, 130, 184 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 137 |
| XapI | RAATTY | 1 cut(s) | 215 |
| XmiI | GTMKAC | 1 cut(s) | 295 |
| XspI | CTAG | 2 cut(s) | 6, 326 |