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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 259 |
| Acc36I | ACCTGC | 1 cut(s) | 259 |
| AccII | CGCG | 1 cut(s) | 337 |
| AciI | CCGC | 7 cut(s) | 180, 337, 345, 391, 499, 519, 534 |
| AclI | AACGTT | 1 cut(s) | 324 |
| AclWI | GGATC | 2 cut(s) | 12, 180 |
| AcoI | YGGCCR | 1 cut(s) | 273 |
| AcsI | RAATTY | 1 cut(s) | 290 |
| AfaI | GTAC | 1 cut(s) | 343 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 214, 268, 397, 400, 442 |
| AjnI | CCWGG | 3 cut(s) | 145, 168, 479 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 296, 328 |
| AluBI | AGCT | 2 cut(s) | 15, 490 |
| AluI | AGCT | 2 cut(s) | 15, 490 |
| AlwI | GGATC | 2 cut(s) | 12, 180 |
| AoxI | GGCC | 6 cut(s) | 166, 252, 258, 273, 373, 515 |
| ApaI | GGGCCC | 1 cut(s) | 377 |
| ApeKI | GCWGC | 1 cut(s) | 150 |
| ApoI | RAATTY | 1 cut(s) | 290 |
| AspLEI | GCGC | 1 cut(s) | 337 |
| AspS9I | GGNCC | 4 cut(s) | 259, 373, 374, 516 |
| AsuHPI | GGTGA | 2 cut(s) | 74, 169 |
| BaeGI | GKGCMC | 1 cut(s) | 377 |
| BalI | TGGCCA | 1 cut(s) | 275 |
| BanII | GRGCYC | 1 cut(s) | 377 |
| BbvI | GCAGC | 1 cut(s) | 162 |
| BccI | CCATC | 2 cut(s) | 3, 15 |
| BciT130I | CCWGG | 3 cut(s) | 147, 170, 481 |
| BclI | TGATCA | 1 cut(s) | 232 |
| BfaI | CTAG | 6 cut(s) | 164, 332, 447, 453, 563, 580 |
| BfuAI | ACCTGC | 1 cut(s) | 259 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 165 |
| BisI | GCNGC | 1 cut(s) | 151 |
| BlsI | GCNGC | 1 cut(s) | 152 |
| Bme1390I | CCNGG | 3 cut(s) | 147, 170, 481 |
| BmgT120I | GGNCC | 4 cut(s) | 259, 373, 374, 516 |
| BmiI | GGNNCC | 1 cut(s) | 375 |
| BmrFI | CCNGG | 3 cut(s) | 147, 170, 481 |
| Bsa29I | ATCGAT | 1 cut(s) | 7 |
| BsaJI | CCNNGG | 2 cut(s) | 255, 479 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 214, 268, 397, 400, 442 |
| BseBI | CCWGG | 3 cut(s) | 147, 170, 481 |
| BseCI | ATCGAT | 1 cut(s) | 7 |
| BseDI | CCNNGG | 2 cut(s) | 255, 479 |
| BseGI | GGATG | 2 cut(s) | 26, 31 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 214, 268, 397, 400, 442 |
| BseSI | GKGCMC | 1 cut(s) | 377 |
| BseXI | GCAGC | 1 cut(s) | 162 |
| BsgI | GTGCAG | 1 cut(s) | 262 |
| Bsh1236I | CGCG | 1 cut(s) | 337 |
| BshFI | GGCC | 6 cut(s) | 168, 254, 260, 275, 375, 517 |
| BshVI | ATCGAT | 1 cut(s) | 7 |
| BslFI | GGGAC | 1 cut(s) | 461 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 214, 268, 397, 400, 442 |
| BsmFI | GGGAC | 1 cut(s) | 461 |
| BsmI | GAATGC | 1 cut(s) | 558 |
| BsnI | GGCC | 6 cut(s) | 168, 254, 260, 275, 375, 517 |
| Bsp120I | GGGCCC | 1 cut(s) | 373 |
| Bsp1286I | GDGCHC | 1 cut(s) | 377 |
| Bsp143I | GATC | 3 cut(s) | 4, 172, 232 |
| BspACI | CCGC | 7 cut(s) | 180, 337, 345, 391, 499, 519, 534 |
| BspANI | GGCC | 6 cut(s) | 168, 254, 260, 275, 375, 517 |
| BspDI | ATCGAT | 1 cut(s) | 7 |
| BspFNI | CGCG | 1 cut(s) | 337 |
| BspLI | GGNNCC | 1 cut(s) | 375 |
| BspMI | ACCTGC | 1 cut(s) | 259 |
| BspPI | GGATC | 2 cut(s) | 12, 180 |
| BssECI | CCNNGG | 2 cut(s) | 255, 479 |
| BssMI | GATC | 3 cut(s) | 4, 172, 232 |
| Bst2UI | CCWGG | 3 cut(s) | 147, 170, 481 |
| BstC8I | GCNNGC | 2 cut(s) | 519, 523 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 212 |
| BstF5I | GGATG | 2 cut(s) | 26, 31 |
| BstFNI | CGCG | 1 cut(s) | 337 |
| BstHHI | GCGC | 1 cut(s) | 337 |
| BstKTI | GATC | 3 cut(s) | 7, 175, 235 |
| BstMBI | GATC | 3 cut(s) | 4, 172, 232 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 156, 165, 317, 496, 518, 527 |
| BstNI | CCWGG | 3 cut(s) | 147, 170, 481 |
| BstSCI | CCNGG | 3 cut(s) | 145, 168, 479 |
| BstSLI | GKGCMC | 1 cut(s) | 377 |
| BstUI | CGCG | 1 cut(s) | 337 |
| BstV1I | GCAGC | 1 cut(s) | 162 |
| BstX2I | RGATCY | 1 cut(s) | 172 |
| BstYI | RGATCY | 1 cut(s) | 172 |
| Bsu15I | ATCGAT | 1 cut(s) | 7 |
| BsuRI | GGCC | 6 cut(s) | 168, 254, 260, 275, 375, 517 |
| BsuTUI | ATCGAT | 1 cut(s) | 7 |
| BtsCI | GGATG | 2 cut(s) | 26, 31 |
| BtsIMutI | CAGTG | 1 cut(s) | 278 |
| BveI | ACCTGC | 1 cut(s) | 259 |
| Cac8I | GCNNGC | 2 cut(s) | 519, 523 |
| CfoI | GCGC | 1 cut(s) | 337 |
| Cfr13I | GGNCC | 4 cut(s) | 259, 373, 374, 516 |
| ClaI | ATCGAT | 1 cut(s) | 7 |
| CseI | GACGC | 1 cut(s) | 536 |
| Csp6I | GTAC | 1 cut(s) | 342 |
| CviAII | CATG | 1 cut(s) | 509 |
| CviQI | GTAC | 1 cut(s) | 342 |
| DpnI | GATC | 3 cut(s) | 6, 174, 234 |
| DpnII | GATC | 3 cut(s) | 4, 172, 232 |
| EaeI | YGGCCR | 1 cut(s) | 273 |
| Eco24I | GRGCYC | 1 cut(s) | 377 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 212 |
| EcoRII | CCWGG | 3 cut(s) | 145, 168, 479 |
| EcoT38I | GRGCYC | 1 cut(s) | 377 |
| FaeI | CATG | 1 cut(s) | 512 |
| FaqI | GGGAC | 1 cut(s) | 461 |
| FatI | CATG | 1 cut(s) | 508 |
| FauI | CCCGC | 2 cut(s) | 398, 526 |
| FbaI | TGATCA | 1 cut(s) | 232 |
| Fnu4HI | GCNGC | 1 cut(s) | 151 |
| FokI | GGATG | 2 cut(s) | 33, 38 |
| FriOI | GRGCYC | 1 cut(s) | 377 |
| Fsp4HI | GCNGC | 1 cut(s) | 151 |
| FspBI | CTAG | 6 cut(s) | 164, 332, 447, 453, 563, 580 |
| GlaI | GCGC | 1 cut(s) | 336 |
| GluI | GCNGC | 1 cut(s) | 151 |
| HaeIII | GGCC | 6 cut(s) | 168, 254, 260, 275, 375, 517 |
| HgaI | GACGC | 1 cut(s) | 536 |
| HhaI | GCGC | 1 cut(s) | 337 |
| Hin1II | CATG | 1 cut(s) | 512 |
| Hin6I | GCGC | 1 cut(s) | 335 |
| HinP1I | GCGC | 1 cut(s) | 335 |
| HincII | GTYRAC | 1 cut(s) | 328 |
| HindII | GTYRAC | 1 cut(s) | 328 |
| HinfI | GANTC | 3 cut(s) | 82, 97, 502 |
| HpaI | GTTAAC | 1 cut(s) | 328 |
| HphI | GGTGA | 2 cut(s) | 74, 169 |
| Hpy166II | GTNNAC | 1 cut(s) | 328 |
| Hpy188I | TCNGA | 1 cut(s) | 136 |
| Hpy188III | TCNNGA | 2 cut(s) | 74, 236 |
| Hpy8I | GTNNAC | 1 cut(s) | 328 |
| Hpy99I | CGWCG | 1 cut(s) | 530 |
| HpyAV | CCTTC | 2 cut(s) | 59, 244 |
| HpyCH4IV | ACGT | 1 cut(s) | 324 |
| HpyCH4V | TGCA | 2 cut(s) | 243, 556 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 156, 165, 317, 496, 518, 527 |
| HpySE526I | ACGT | 1 cut(s) | 324 |
| Hsp92II | CATG | 1 cut(s) | 512 |
| HspAI | GCGC | 1 cut(s) | 335 |
| Ksp22I | TGATCA | 1 cut(s) | 232 |
| KspAI | GTTAAC | 1 cut(s) | 328 |
| Kzo9I | GATC | 3 cut(s) | 4, 172, 232 |
| LmnI | GCTCC | 2 cut(s) | 12, 226 |
| Lsp1109I | GCAGC | 1 cut(s) | 162 |
| MaeI | CTAG | 6 cut(s) | 164, 332, 447, 453, 563, 580 |
| MaeII | ACGT | 1 cut(s) | 324 |
| MaeIII | GTNAC | 2 cut(s) | 110, 417 |
| MalI | GATC | 3 cut(s) | 6, 174, 234 |
| MboI | GATC | 3 cut(s) | 4, 172, 232 |
| MflI | RGATCY | 1 cut(s) | 172 |
| MhlI | GDGCHC | 1 cut(s) | 377 |
| MlsI | TGGCCA | 1 cut(s) | 275 |
| MluCI | AATT | 1 cut(s) | 290 |
| MluNI | TGGCCA | 1 cut(s) | 275 |
| MlyI | GAGTC | 1 cut(s) | 106 |
| MmeI | TCCRAC | 1 cut(s) | 294 |
| MnlI | CCTC | 7 cut(s) | 218, 250, 310, 390, 446, 454, 507 |
| Mox20I | TGGCCA | 1 cut(s) | 275 |
| MscI | TGGCCA | 1 cut(s) | 275 |
| MseI | TTAA | 2 cut(s) | 327, 432 |
| MslI | CAYNNNNRTG | 1 cut(s) | 281 |
| Msp20I | TGGCCA | 1 cut(s) | 275 |
| MspA1I | CMGCKG | 2 cut(s) | 499, 521 |
| MspR9I | CCNGG | 3 cut(s) | 147, 170, 481 |
| Mva1269I | GAATGC | 1 cut(s) | 558 |
| MvaI | CCWGG | 3 cut(s) | 147, 170, 481 |
| MvnI | CGCG | 1 cut(s) | 337 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 156, 165, 317, 496, 518, 527 |
| NdeII | GATC | 3 cut(s) | 4, 172, 232 |
| NlaIII | CATG | 1 cut(s) | 512 |
| NlaIV | GGNNCC | 1 cut(s) | 375 |
| NmeAIII | GCCGAG | 2 cut(s) | 118, 280 |
| NmuCI | GTSAC | 1 cut(s) | 417 |
| PaqCI | CACCTGC | 1 cut(s) | 259 |
| PctI | GAATGC | 1 cut(s) | 558 |
| PfeI | GAWTC | 2 cut(s) | 82, 502 |
| PkrI | GCNGC | 1 cut(s) | 152 |
| PleI | GAGTC | 1 cut(s) | 105 |
| PpsI | GAGTC | 1 cut(s) | 105 |
| Psp1406I | AACGTT | 1 cut(s) | 324 |
| Psp6I | CCWGG | 3 cut(s) | 145, 168, 479 |
| PspGI | CCWGG | 3 cut(s) | 145, 168, 479 |
| PspN4I | GGNNCC | 1 cut(s) | 375 |
| PspOMI | GGGCCC | 1 cut(s) | 373 |
| PspPI | GGNCC | 4 cut(s) | 259, 373, 374, 516 |
| PsuI | RGATCY | 1 cut(s) | 172 |
| RsaI | GTAC | 1 cut(s) | 343 |
| RsaNI | GTAC | 1 cut(s) | 342 |
| RseI | CAYNNNNRTG | 1 cut(s) | 281 |
| SaqAI | TTAA | 2 cut(s) | 327, 432 |
| SatI | GCNGC | 1 cut(s) | 151 |
| Sau3AI | GATC | 3 cut(s) | 4, 172, 232 |
| Sau96I | GGNCC | 4 cut(s) | 259, 373, 374, 516 |
| SchI | GAGTC | 1 cut(s) | 106 |
| ScrFI | CCNGG | 3 cut(s) | 147, 170, 481 |
| SduI | GDGCHC | 1 cut(s) | 377 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 281 |
| Sse9I | AATT | 1 cut(s) | 290 |
| SsiI | CCGC | 7 cut(s) | 180, 337, 345, 391, 499, 519, 534 |
| SspMI | CTAG | 6 cut(s) | 164, 332, 447, 453, 563, 580 |
| StyD4I | CCNGG | 3 cut(s) | 145, 168, 479 |
| TaiI | ACGT | 1 cut(s) | 327 |
| TaqI | TCGA | 2 cut(s) | 7, 294 |
| TasI | AATT | 1 cut(s) | 290 |
| TfiI | GAWTC | 2 cut(s) | 82, 502 |
| Tru1I | TTAA | 2 cut(s) | 327, 432 |
| Tru9I | TTAA | 2 cut(s) | 327, 432 |
| TscAI | CASTG | 1 cut(s) | 285 |
| TseFI | GTSAC | 1 cut(s) | 417 |
| TseI | GCWGC | 1 cut(s) | 150 |
| Tsp45I | GTSAC | 1 cut(s) | 417 |
| TspRI | CASTG | 1 cut(s) | 285 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 212 |
| XapI | RAATTY | 1 cut(s) | 290 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 487 |
| XspI | CTAG | 6 cut(s) | 164, 332, 447, 453, 563, 580 |