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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 74, 266 |
| AclWI | GGATC | 2 cut(s) | 168, 201 |
| AcoI | YGGCCR | 3 cut(s) | 108, 261, 306 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 1 cut(s) | 176 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 77 |
| AflIII | ACRYGT | 1 cut(s) | 275 |
| AjnI | CCWGG | 3 cut(s) | 133, 156, 347 |
| AluBI | AGCT | 1 cut(s) | 43 |
| AluI | AGCT | 1 cut(s) | 43 |
| Alw21I | GWGCWC | 1 cut(s) | 45 |
| AlwI | GGATC | 2 cut(s) | 168, 201 |
| AoxI | GGCC | 5 cut(s) | 108, 154, 240, 261, 306 |
| ApeKI | GCWGC | 3 cut(s) | 138, 141, 342 |
| AspLEI | GCGC | 2 cut(s) | 231, 268 |
| AsuC2I | CCSGG | 3 cut(s) | 107, 117, 320 |
| AsuHPI | GGTGA | 1 cut(s) | 134 |
| BanII | GRGCYC | 1 cut(s) | 45 |
| Bbv12I | GWGCWC | 1 cut(s) | 45 |
| BbvI | GCAGC | 3 cut(s) | 150, 153, 354 |
| BccI | CCATC | 1 cut(s) | 253 |
| BciT130I | CCWGG | 3 cut(s) | 135, 158, 349 |
| BcnI | CCSGG | 3 cut(s) | 107, 117, 320 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 306, 343 |
| BlsI | GCNGC | 6 cut(s) | 140, 143, 146, 265, 307, 344 |
| Bme1390I | CCNGG | 6 cut(s) | 107, 117, 135, 158, 320, 349 |
| BmrFI | CCNGG | 6 cut(s) | 107, 117, 135, 158, 320, 349 |
| BpuMI | CCSGG | 3 cut(s) | 107, 117, 320 |
| BsaJI | CCNNGG | 2 cut(s) | 105, 115 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 77 |
| Bse1I | ACTGG | 1 cut(s) | 192 |
| BseBI | CCWGG | 3 cut(s) | 135, 158, 349 |
| BseDI | CCNNGG | 2 cut(s) | 105, 115 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 77 |
| BseNI | ACTGG | 1 cut(s) | 192 |
| BseXI | GCAGC | 3 cut(s) | 150, 153, 354 |
| Bsh1236I | CGCG | 2 cut(s) | 74, 266 |
| Bsh1285I | CGRYCG | 2 cut(s) | 78, 316 |
| BshFI | GGCC | 5 cut(s) | 110, 156, 242, 263, 308 |
| BsiEI | CGRYCG | 2 cut(s) | 78, 316 |
| BsiHKAI | GWGCWC | 1 cut(s) | 45 |
| BsiSI | CCGG | 3 cut(s) | 107, 117, 320 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 77 |
| BsnI | GGCC | 5 cut(s) | 110, 156, 242, 263, 308 |
| Bsp1286I | GDGCHC | 1 cut(s) | 45 |
| Bsp143I | GATC | 3 cut(s) | 75, 160, 193 |
| BspANI | GGCC | 5 cut(s) | 110, 156, 242, 263, 308 |
| BspFNI | CGCG | 2 cut(s) | 74, 266 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspPI | GGATC | 2 cut(s) | 168, 201 |
| BspQI | GCTCTTC | 2 cut(s) | 24, 219 |
| BsrI | ACTGG | 1 cut(s) | 192 |
| BssECI | CCNNGG | 2 cut(s) | 105, 115 |
| BssMI | GATC | 3 cut(s) | 75, 160, 193 |
| Bst2UI | CCWGG | 3 cut(s) | 135, 158, 349 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 219 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 208, 332 |
| BstFNI | CGCG | 2 cut(s) | 74, 266 |
| BstHHI | GCGC | 2 cut(s) | 231, 268 |
| BstKTI | GATC | 3 cut(s) | 78, 163, 196 |
| BstMBI | GATC | 3 cut(s) | 75, 160, 193 |
| BstMCI | CGRYCG | 2 cut(s) | 78, 316 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 40, 87, 144, 153 |
| BstNI | CCWGG | 3 cut(s) | 135, 158, 349 |
| BstSCI | CCNGG | 6 cut(s) | 105, 115, 133, 156, 318, 347 |
| BstUI | CGCG | 2 cut(s) | 74, 266 |
| BstV1I | GCAGC | 3 cut(s) | 150, 153, 354 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 5 cut(s) | 110, 156, 242, 263, 308 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 208, 332 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 2 cut(s) | 231, 268 |
| Csp6I | GTAC | 1 cut(s) | 175 |
| CviAII | CATG | 2 cut(s) | 172, 271 |
| CviJI | RGCY | 9 cut(s) | 5, 43, 110, 144, 156, 242, 248, 263, 308 |
| CviKI_1 | RGCY | 9 cut(s) | 5, 43, 110, 144, 156, 242, 248, 263, 308 |
| CviQI | GTAC | 1 cut(s) | 175 |
| DpnI | GATC | 3 cut(s) | 77, 162, 195 |
| DpnII | GATC | 3 cut(s) | 75, 160, 193 |
| EaeI | YGGCCR | 3 cut(s) | 108, 261, 306 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 219 |
| EarI | CTCTTC | 2 cut(s) | 24, 219 |
| EciI | GGCGGA | 1 cut(s) | 206 |
| Ecl136II | GAGCTC | 1 cut(s) | 43 |
| Eco24I | GRGCYC | 1 cut(s) | 45 |
| Eco53kI | GAGCTC | 1 cut(s) | 43 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| EcoICRI | GAGCTC | 1 cut(s) | 43 |
| EcoRII | CCWGG | 3 cut(s) | 133, 156, 347 |
| EcoT38I | GRGCYC | 1 cut(s) | 45 |
| FaeI | CATG | 2 cut(s) | 175, 274 |
| FaiI | YATR | 3 cut(s) | 173, 201, 272 |
| FatI | CATG | 2 cut(s) | 171, 270 |
| FauI | CCCGC | 3 cut(s) | 8, 55, 79 |
| Fnu4HI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 306, 343 |
| FriOI | GRGCYC | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 306, 343 |
| GlaI | GCGC | 2 cut(s) | 230, 267 |
| GluI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 306, 343 |
| HaeIII | GGCC | 5 cut(s) | 110, 156, 242, 263, 308 |
| HapII | CCGG | 3 cut(s) | 107, 117, 320 |
| HhaI | GCGC | 2 cut(s) | 231, 268 |
| Hin1II | CATG | 2 cut(s) | 175, 274 |
| Hin6I | GCGC | 2 cut(s) | 229, 266 |
| HinP1I | GCGC | 2 cut(s) | 229, 266 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 3 cut(s) | 107, 117, 320 |
| HphI | GGTGA | 1 cut(s) | 134 |
| Hpy166II | GTNNAC | 1 cut(s) | 85 |
| Hpy188I | TCNGA | 1 cut(s) | 67 |
| Hpy188III | TCNNGA | 1 cut(s) | 271 |
| Hpy8I | GTNNAC | 1 cut(s) | 85 |
| HpyAV | CCTTC | 1 cut(s) | 232 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 1 cut(s) | 277 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 40, 87, 144, 153 |
| HpySE526I | ACGT | 1 cut(s) | 277 |
| Hsp92II | CATG | 2 cut(s) | 175, 274 |
| HspAI | GCGC | 2 cut(s) | 229, 266 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 3 cut(s) | 75, 160, 193 |
| LguI | GCTCTTC | 2 cut(s) | 24, 219 |
| LmnI | GCTCC | 2 cut(s) | 48, 214 |
| Lsp1109I | GCAGC | 3 cut(s) | 150, 153, 354 |
| MaeII | ACGT | 1 cut(s) | 277 |
| MaeIII | GTNAC | 1 cut(s) | 336 |
| MalI | GATC | 3 cut(s) | 77, 162, 195 |
| MboI | GATC | 3 cut(s) | 75, 160, 193 |
| MboII | GAAGA | 3 cut(s) | 41, 188, 236 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MhlI | GDGCHC | 1 cut(s) | 45 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| MnlI | CCTC | 4 cut(s) | 73, 118, 214, 277 |
| MseI | TTAA | 1 cut(s) | 84 |
| MspI | CCGG | 3 cut(s) | 107, 117, 320 |
| MspR9I | CCNGG | 6 cut(s) | 107, 117, 135, 158, 320, 349 |
| MvaI | CCWGG | 3 cut(s) | 135, 158, 349 |
| MvnI | CGCG | 2 cut(s) | 74, 266 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 40, 87, 144, 153 |
| NciI | CCSGG | 3 cut(s) | 107, 117, 320 |
| NdeII | GATC | 3 cut(s) | 75, 160, 193 |
| NlaIII | CATG | 2 cut(s) | 175, 274 |
| NmuCI | GTSAC | 1 cut(s) | 336 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PciSI | GCTCTTC | 2 cut(s) | 24, 219 |
| PkrI | GCNGC | 6 cut(s) | 140, 143, 146, 265, 307, 344 |
| Ple19I | CGATCG | 1 cut(s) | 78 |
| Psp124BI | GAGCTC | 1 cut(s) | 45 |
| Psp6I | CCWGG | 3 cut(s) | 133, 156, 347 |
| PspGI | CCWGG | 3 cut(s) | 133, 156, 347 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PvuI | CGATCG | 1 cut(s) | 78 |
| RsaI | GTAC | 1 cut(s) | 176 |
| RsaNI | GTAC | 1 cut(s) | 175 |
| SacI | GAGCTC | 1 cut(s) | 45 |
| SapI | GCTCTTC | 2 cut(s) | 24, 219 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 306, 343 |
| Sau3AI | GATC | 3 cut(s) | 75, 160, 193 |
| ScrFI | CCNGG | 6 cut(s) | 107, 117, 135, 158, 320, 349 |
| SduI | GDGCHC | 1 cut(s) | 45 |
| SetI | ASST | 3 cut(s) | 45, 65, 280 |
| SstI | GAGCTC | 1 cut(s) | 45 |
| StyD4I | CCNGG | 6 cut(s) | 105, 115, 133, 156, 318, 347 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 1 cut(s) | 280 |
| TauI | GCSGC | 3 cut(s) | 147, 266, 308 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 1 cut(s) | 336 |
| TseI | GCWGC | 3 cut(s) | 138, 141, 342 |
| Tsp45I | GTSAC | 1 cut(s) | 336 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspRI | CASTG | 1 cut(s) | 192 |