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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 538 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 487 |
| Acc16I | TGCGCA | 1 cut(s) | 615 |
| Acc36I | ACCTGC | 1 cut(s) | 538 |
| Acc65I | GGTACC | 1 cut(s) | 670 |
| AccB1I | GGYRCC | 3 cut(s) | 589, 670, 946 |
| AccBSI | CCGCTC | 3 cut(s) | 197, 213, 792 |
| AccI | GTMKAC | 1 cut(s) | 348 |
| AccII | CGCG | 3 cut(s) | 301, 510, 631 |
| AclWI | GGATC | 5 cut(s) | 331, 344, 465, 1002, 1015 |
| AcoI | YGGCCR | 3 cut(s) | 43, 310, 342 |
| AcsI | RAATTY | 1 cut(s) | 569 |
| AcyI | GRCGYC | 2 cut(s) | 590, 947 |
| AfaI | GTAC | 4 cut(s) | 672, 686, 697, 1029 |
| AgeI | ACCGGT | 1 cut(s) | 144 |
| AgsI | TTSAA | 2 cut(s) | 246, 364 |
| AjiI | CACGTC | 2 cut(s) | 499, 979 |
| AjnI | CCWGG | 1 cut(s) | 1003 |
| AluBI | AGCT | 1 cut(s) | 1064 |
| AluI | AGCT | 1 cut(s) | 1064 |
| Alw21I | GWGCWC | 3 cut(s) | 20, 230, 638 |
| AlwI | GGATC | 5 cut(s) | 331, 344, 465, 1002, 1015 |
| Ama87I | CYCGRG | 2 cut(s) | 13, 848 |
| ApaI | GGGCCC | 2 cut(s) | 731, 873 |
| ApeKI | GCWGC | 1 cut(s) | 1064 |
| ApoI | RAATTY | 1 cut(s) | 569 |
| AsiGI | ACCGGT | 1 cut(s) | 144 |
| Asp718I | GGTACC | 1 cut(s) | 670 |
| AspLEI | GCGC | 8 cut(s) | 139, 301, 325, 510, 522, 592, 616, 949 |
| AsuC2I | CCSGG | 7 cut(s) | 223, 341, 377, 439, 863, 899, 952 |
| AsuHPI | GGTGA | 5 cut(s) | 191, 250, 292, 450, 990 |
| AvaI | CYCGRG | 2 cut(s) | 13, 848 |
| AvaII | GGWCC | 5 cut(s) | 147, 235, 676, 954, 991 |
| BaeGI | GKGCMC | 2 cut(s) | 731, 873 |
| BamHI | GGATCC | 2 cut(s) | 336, 1007 |
| BanI | GGYRCC | 3 cut(s) | 589, 670, 946 |
| BanII | GRGCYC | 3 cut(s) | 8, 731, 873 |
| Bbv12I | GWGCWC | 3 cut(s) | 20, 230, 638 |
| BbvI | GCAGC | 1 cut(s) | 1051 |
| BccI | CCATC | 8 cut(s) | 113, 196, 296, 321, 422, 483, 718, 1027 |
| BceAI | ACGGC | 4 cut(s) | 58, 234, 329, 1010 |
| BciT130I | CCWGG | 1 cut(s) | 1005 |
| BcnI | CCSGG | 7 cut(s) | 223, 341, 377, 439, 863, 899, 952 |
| BfaI | CTAG | 4 cut(s) | 60, 296, 462, 917 |
| BfoI | RGCGCY | 3 cut(s) | 326, 593, 950 |
| BfuAI | ACCTGC | 1 cut(s) | 538 |
| BisI | GCNGC | 4 cut(s) | 211, 381, 945, 1065 |
| BlpI | GCTNAGC | 1 cut(s) | 807 |
| BlsI | GCNGC | 4 cut(s) | 212, 382, 946, 1066 |
| Bme1390I | CCNGG | 8 cut(s) | 223, 341, 377, 439, 863, 899, 952, 1005 |
| Bme18I | GGWCC | 5 cut(s) | 147, 235, 676, 954, 991 |
| BmeT110I | CYCGRG | 2 cut(s) | 13, 848 |
| BmgBI | CACGTC | 2 cut(s) | 499, 979 |
| BmrFI | CCNGG | 8 cut(s) | 223, 341, 377, 439, 863, 899, 952, 1005 |
| BmsI | GCATC | 1 cut(s) | 897 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 807 |
| BpuEI | CTTGAG | 2 cut(s) | 162, 776 |
| BpuMI | CCSGG | 7 cut(s) | 223, 341, 377, 439, 863, 899, 952 |
| BsaAI | YACGTR | 2 cut(s) | 688, 1031 |
| BsaHI | GRCGYC | 2 cut(s) | 590, 947 |
| BsaJI | CCNNGG | 7 cut(s) | 216, 437, 492, 812, 830, 950, 982 |
| BsaWI | WCCGGW | 3 cut(s) | 144, 333, 673 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 357, 387, 755, 785 |
| Bse118I | RCCGGY | 4 cut(s) | 144, 308, 659, 875 |
| BseBI | CCWGG | 1 cut(s) | 1005 |
| BseDI | CCNNGG | 7 cut(s) | 216, 437, 492, 812, 830, 950, 982 |
| BseGI | GGATG | 7 cut(s) | 288, 313, 414, 475, 729, 912, 1019 |
| BseMII | CTCAG | 1 cut(s) | 798 |
| BseSI | GKGCMC | 2 cut(s) | 731, 873 |
| BseX3I | CGGCCG | 1 cut(s) | 342 |
| BseXI | GCAGC | 1 cut(s) | 1051 |
| Bsh1236I | CGCG | 3 cut(s) | 301, 510, 631 |
| Bsh1285I | CGRYCG | 3 cut(s) | 305, 309, 345 |
| BshNI | GGYRCC | 3 cut(s) | 589, 670, 946 |
| BshTI | ACCGGT | 1 cut(s) | 144 |
| BsiEI | CGRYCG | 3 cut(s) | 305, 309, 345 |
| BsiHKAI | GWGCWC | 3 cut(s) | 20, 230, 638 |
| BsiHKCI | CYCGRG | 2 cut(s) | 13, 848 |
| BsiWI | CGTACG | 2 cut(s) | 684, 1027 |
| BslFI | GGGAC | 1 cut(s) | 1004 |
| BsmFI | GGGAC | 1 cut(s) | 1004 |
| BsoBI | CYCGRG | 2 cut(s) | 13, 848 |
| Bsp120I | GGGCCC | 2 cut(s) | 727, 869 |
| Bsp1286I | GDGCHC | 6 cut(s) | 8, 20, 230, 638, 731, 873 |
| Bsp143I | GATC | 7 cut(s) | 255, 269, 302, 336, 455, 470, 1007 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 807 |
| Bsp19I | CCATGG | 1 cut(s) | 492 |
| BspCNI | CTCAG | 1 cut(s) | 799 |
| BspFNI | CGCG | 3 cut(s) | 301, 510, 631 |
| BspMI | ACCTGC | 1 cut(s) | 538 |
| BspPI | GGATC | 5 cut(s) | 331, 344, 465, 1002, 1015 |
| BspT107I | GGYRCC | 3 cut(s) | 589, 670, 946 |
| BsrBI | CCGCTC | 3 cut(s) | 197, 213, 792 |
| BsrFI | RCCGGY | 4 cut(s) | 144, 308, 659, 875 |
| BssAI | RCCGGY | 4 cut(s) | 144, 308, 659, 875 |
| BssECI | CCNNGG | 7 cut(s) | 216, 437, 492, 812, 830, 950, 982 |
| BssMI | GATC | 7 cut(s) | 255, 269, 302, 336, 455, 470, 1007 |
| BssNI | GRCGYC | 2 cut(s) | 590, 947 |
| BssT1I | CCWWGG | 2 cut(s) | 492, 830 |
| Bst2UI | CCWGG | 1 cut(s) | 1005 |
| Bst4CI | ACNGT | 3 cut(s) | 132, 161, 235 |
| Bst6I | CTCTTC | 1 cut(s) | 978 |
| BstACI | GRCGYC | 2 cut(s) | 590, 947 |
| BstBAI | YACGTR | 2 cut(s) | 688, 1031 |
| BstC8I | GCNNGC | 5 cut(s) | 623, 845, 873, 877, 1016 |
| BstDEI | CTNAG | 1 cut(s) | 807 |
| BstDSI | CCRYGG | 2 cut(s) | 216, 492 |
| BstF5I | GGATG | 7 cut(s) | 288, 313, 414, 475, 729, 912, 1019 |
| BstFNI | CGCG | 3 cut(s) | 301, 510, 631 |
| BstH2I | RGCGCY | 3 cut(s) | 326, 593, 950 |
| BstHHI | GCGC | 8 cut(s) | 139, 301, 325, 510, 522, 592, 616, 949 |
| BstKTI | GATC | 7 cut(s) | 258, 272, 305, 339, 458, 473, 1010 |
| BstMBI | GATC | 7 cut(s) | 255, 269, 302, 336, 455, 470, 1007 |
| BstMCI | CGRYCG | 3 cut(s) | 305, 309, 345 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 104, 596, 622, 803, 872, 881, 944 |
| BstNI | CCWGG | 1 cut(s) | 1005 |
| BstNSI | RCATGY | 1 cut(s) | 1018 |
| BstSCI | CCNGG | 8 cut(s) | 221, 339, 375, 437, 861, 897, 950, 1003 |
| BstSLI | GKGCMC | 2 cut(s) | 731, 873 |
| BstSNI | TACGTA | 2 cut(s) | 688, 1031 |
| BstUI | CGCG | 3 cut(s) | 301, 510, 631 |
| BstV1I | GCAGC | 1 cut(s) | 1051 |
| BstX2I | RGATCY | 3 cut(s) | 336, 470, 1007 |
| BstYI | RGATCY | 3 cut(s) | 336, 470, 1007 |
| BstZI | CGGCCG | 1 cut(s) | 342 |
| BtgI | CCRYGG | 2 cut(s) | 216, 492 |
| BtrI | CACGTC | 2 cut(s) | 499, 979 |
| BtsCI | GGATG | 7 cut(s) | 288, 313, 414, 475, 729, 912, 1019 |
| BveI | ACCTGC | 1 cut(s) | 538 |
| Cac8I | GCNNGC | 5 cut(s) | 623, 845, 873, 877, 1016 |
| CfoI | GCGC | 8 cut(s) | 139, 301, 325, 510, 522, 592, 616, 949 |
| Cfr10I | RCCGGY | 4 cut(s) | 144, 308, 659, 875 |
| CpoI | CGGWCCG | 1 cut(s) | 235 |
| CseI | GACGC | 1 cut(s) | 890 |
| Csp6I | GTAC | 4 cut(s) | 671, 685, 696, 1028 |
| CspAI | ACCGGT | 1 cut(s) | 144 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 97, 132, 812, 847 |
| CspI | CGGWCCG | 1 cut(s) | 235 |
| CviAII | CATG | 4 cut(s) | 493, 515, 1015, 1068 |
| CviQI | GTAC | 4 cut(s) | 671, 685, 696, 1028 |
| DdeI | CTNAG | 1 cut(s) | 807 |
| DinI | GGCGCC | 2 cut(s) | 591, 948 |
| DpnI | GATC | 7 cut(s) | 257, 271, 304, 338, 457, 472, 1009 |
| DpnII | GATC | 7 cut(s) | 255, 269, 302, 336, 455, 470, 1007 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 487 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 487 |
| EaeI | YGGCCR | 3 cut(s) | 43, 310, 342 |
| EagI | CGGCCG | 1 cut(s) | 342 |
| Eam1104I | CTCTTC | 1 cut(s) | 978 |
| EarI | CTCTTC | 1 cut(s) | 978 |
| EciI | GGCGGA | 5 cut(s) | 227, 548, 678, 953, 1012 |
| EclXI | CGGCCG | 1 cut(s) | 342 |
| Eco105I | TACGTA | 2 cut(s) | 688, 1031 |
| Eco130I | CCWWGG | 2 cut(s) | 492, 830 |
| Eco147I | AGGCCT | 1 cut(s) | 58 |
| Eco24I | GRGCYC | 3 cut(s) | 8, 731, 873 |
| Eco47I | GGWCC | 5 cut(s) | 147, 235, 676, 954, 991 |
| Eco52I | CGGCCG | 1 cut(s) | 342 |
| Eco88I | CYCGRG | 2 cut(s) | 13, 848 |
| EcoO109I | RGGNCCY | 1 cut(s) | 869 |
| EcoRII | CCWGG | 1 cut(s) | 1003 |
| EcoT14I | CCWWGG | 2 cut(s) | 492, 830 |
| EcoT38I | GRGCYC | 3 cut(s) | 8, 731, 873 |
| EgeI | GGCGCC | 2 cut(s) | 591, 948 |
| EheI | GGCGCC | 2 cut(s) | 591, 948 |
| ErhI | CCWWGG | 2 cut(s) | 492, 830 |
| FaeI | CATG | 4 cut(s) | 496, 518, 1018, 1071 |
| FaiI | YATR | 6 cut(s) | 494, 516, 691, 1016, 1034, 1069 |
| FaqI | GGGAC | 1 cut(s) | 1004 |
| FatI | CATG | 4 cut(s) | 492, 514, 1014, 1067 |
| FauI | CCCGC | 7 cut(s) | 79, 95, 215, 360, 859, 880, 1019 |
| FblI | GTMKAC | 1 cut(s) | 348 |
| Fnu4HI | GCNGC | 4 cut(s) | 211, 381, 945, 1065 |
| FokI | GGATG | 7 cut(s) | 275, 300, 401, 462, 736, 919, 1006 |
| FriOI | GRGCYC | 3 cut(s) | 8, 731, 873 |
| Fsp4HI | GCNGC | 4 cut(s) | 211, 381, 945, 1065 |
| FspBI | CTAG | 4 cut(s) | 60, 296, 462, 917 |
| FspI | TGCGCA | 1 cut(s) | 615 |
| GlaI | GCGC | 8 cut(s) | 138, 300, 324, 509, 521, 591, 615, 948 |
| GluI | GCNGC | 4 cut(s) | 211, 381, 945, 1065 |
| HaeII | RGCGCY | 3 cut(s) | 326, 593, 950 |
| HgaI | GACGC | 1 cut(s) | 890 |
| HhaI | GCGC | 8 cut(s) | 139, 301, 325, 510, 522, 592, 616, 949 |
| Hin1I | GRCGYC | 2 cut(s) | 590, 947 |
| Hin1II | CATG | 4 cut(s) | 496, 518, 1018, 1071 |
| Hin6I | GCGC | 8 cut(s) | 137, 299, 323, 508, 520, 590, 614, 947 |
| HinP1I | GCGC | 8 cut(s) | 137, 299, 323, 508, 520, 590, 614, 947 |
| HincII | GTYRAC | 2 cut(s) | 164, 349 |
| HindII | GTYRAC | 2 cut(s) | 164, 349 |
| HinfI | GANTC | 3 cut(s) | 37, 578, 770 |
| HphI | GGTGA | 5 cut(s) | 191, 250, 292, 450, 990 |
| Hpy166II | GTNNAC | 3 cut(s) | 164, 283, 349 |
| Hpy188I | TCNGA | 3 cut(s) | 419, 480, 983 |
| Hpy188III | TCNNGA | 3 cut(s) | 250, 317, 989 |
| Hpy8I | GTNNAC | 3 cut(s) | 164, 283, 349 |
| Hpy99I | CGWCG | 7 cut(s) | 105, 136, 235, 256, 350, 484, 884 |
| HpyAV | CCTTC | 2 cut(s) | 523, 580 |
| HpyCH4III | ACNGT | 3 cut(s) | 132, 161, 235 |
| HpyCH4IV | ACGT | 6 cut(s) | 488, 498, 557, 687, 978, 1030 |
| HpyCH4V | TGCA | 4 cut(s) | 693, 910, 1036, 1067 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 104, 596, 622, 803, 872, 881, 944 |
| HpyF3I | CTNAG | 1 cut(s) | 807 |
| HpySE526I | ACGT | 6 cut(s) | 488, 498, 557, 687, 978, 1030 |
| Hsp92I | GRCGYC | 2 cut(s) | 590, 947 |
| Hsp92II | CATG | 4 cut(s) | 496, 518, 1018, 1071 |
| HspAI | GCGC | 8 cut(s) | 137, 299, 323, 508, 520, 590, 614, 947 |
| KasI | GGCGCC | 2 cut(s) | 589, 946 |
| KpnI | GGTACC | 1 cut(s) | 674 |
| KroI | GCCGGC | 1 cut(s) | 875 |
| KroNI | GCCGGC | 1 cut(s) | 877 |
| Kzo9I | GATC | 7 cut(s) | 255, 269, 302, 336, 455, 470, 1007 |
| LmnI | GCTCC | 3 cut(s) | 190, 218, 633 |
| Lsp1109I | GCAGC | 1 cut(s) | 1051 |
| LweI | GCATC | 1 cut(s) | 897 |
| MaeI | CTAG | 4 cut(s) | 60, 296, 462, 917 |
| MaeII | ACGT | 6 cut(s) | 488, 498, 557, 687, 978, 1030 |
| MaeIII | GTNAC | 4 cut(s) | 38, 553, 771, 1052 |
| MalI | GATC | 7 cut(s) | 257, 271, 304, 338, 457, 472, 1009 |
| MbiI | CCGCTC | 3 cut(s) | 197, 213, 792 |
| MboI | GATC | 7 cut(s) | 255, 269, 302, 336, 455, 470, 1007 |
| MboII | GAAGA | 2 cut(s) | 595, 965 |
| MflI | RGATCY | 3 cut(s) | 336, 470, 1007 |
| MhlI | GDGCHC | 6 cut(s) | 8, 20, 230, 638, 731, 873 |
| MluCI | AATT | 3 cut(s) | 359, 569, 930 |
| Mly113I | GGCGCC | 2 cut(s) | 590, 947 |
| MlyI | GAGTC | 3 cut(s) | 46, 587, 779 |
| MmeI | TCCRAC | 3 cut(s) | 316, 442, 503 |
| MreI | CGCCGGCG | 1 cut(s) | 875 |
| MroNI | GCCGGC | 1 cut(s) | 875 |
| MseI | TTAA | 1 cut(s) | 893 |
| MslI | CAYNNNNRTG | 2 cut(s) | 497, 560 |
| MspA1I | CMGCKG | 1 cut(s) | 1064 |
| MspR9I | CCNGG | 8 cut(s) | 223, 341, 377, 439, 863, 899, 952, 1005 |
| MvaI | CCWGG | 1 cut(s) | 1005 |
| MvnI | CGCG | 3 cut(s) | 301, 510, 631 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 104, 596, 622, 803, 872, 881, 944 |
| NaeI | GCCGGC | 1 cut(s) | 877 |
| NarI | GGCGCC | 2 cut(s) | 590, 947 |
| NciI | CCSGG | 7 cut(s) | 223, 341, 377, 439, 863, 899, 952 |
| NcoI | CCATGG | 1 cut(s) | 492 |
| NdeII | GATC | 7 cut(s) | 255, 269, 302, 336, 455, 470, 1007 |
| NgoMIV | GCCGGC | 1 cut(s) | 875 |
| NlaIII | CATG | 4 cut(s) | 496, 518, 1018, 1071 |
| NmeAIII | GCCGAG | 2 cut(s) | 773, 837 |
| NmuCI | GTSAC | 4 cut(s) | 38, 553, 771, 1052 |
| NsbI | TGCGCA | 1 cut(s) | 615 |
| NspI | RCATGY | 1 cut(s) | 1018 |
| PaeI | GCATGC | 1 cut(s) | 1018 |
| PaqCI | CACCTGC | 1 cut(s) | 538 |
| PceI | AGGCCT | 1 cut(s) | 58 |
| PdiI | GCCGGC | 1 cut(s) | 877 |
| Pfl23II | CGTACG | 2 cut(s) | 684, 1027 |
| PflFI | GACNNNGTC | 1 cut(s) | 487 |
| PfoI | TCCNGGA | 1 cut(s) | 897 |
| PinAI | ACCGGT | 1 cut(s) | 144 |
| PkrI | GCNGC | 4 cut(s) | 212, 382, 946, 1066 |
| Ple19I | CGATCG | 1 cut(s) | 305 |
| PleI | GAGTC | 3 cut(s) | 45, 586, 778 |
| PluTI | GGCGCC | 2 cut(s) | 593, 950 |
| PpsI | GAGTC | 3 cut(s) | 45, 586, 778 |
| Ppu21I | YACGTR | 2 cut(s) | 688, 1031 |
| Psp6I | CCWGG | 1 cut(s) | 1003 |
| PspGI | CCWGG | 1 cut(s) | 1003 |
| PspLI | CGTACG | 2 cut(s) | 684, 1027 |
| PspOMI | GGGCCC | 2 cut(s) | 727, 869 |
| PsuI | RGATCY | 3 cut(s) | 336, 470, 1007 |
| PsyI | GACNNNGTC | 1 cut(s) | 487 |
| PvuI | CGATCG | 1 cut(s) | 305 |
| PvuII | CAGCTG | 1 cut(s) | 1064 |
| RsaI | GTAC | 4 cut(s) | 672, 686, 697, 1029 |
| RsaNI | GTAC | 4 cut(s) | 671, 685, 696, 1028 |
| RseI | CAYNNNNRTG | 2 cut(s) | 497, 560 |
| Rsr2I | CGGWCCG | 1 cut(s) | 235 |
| RsrII | CGGWCCG | 1 cut(s) | 235 |
| SalI | GTCGAC | 1 cut(s) | 347 |
| SaqAI | TTAA | 1 cut(s) | 893 |
| SatI | GCNGC | 4 cut(s) | 211, 381, 945, 1065 |
| Sau3AI | GATC | 7 cut(s) | 255, 269, 302, 336, 455, 470, 1007 |
| SchI | GAGTC | 3 cut(s) | 46, 587, 779 |
| ScrFI | CCNGG | 8 cut(s) | 223, 341, 377, 439, 863, 899, 952, 1005 |
| SduI | GDGCHC | 6 cut(s) | 8, 20, 230, 638, 731, 873 |
| SfaNI | GCATC | 1 cut(s) | 897 |
| SfoI | GGCGCC | 2 cut(s) | 591, 948 |
| SgrAI | CRCCGGYG | 2 cut(s) | 659, 875 |
| SinI | GGWCC | 5 cut(s) | 147, 235, 676, 954, 991 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 497, 560 |
| SmlI | CTYRAG | 2 cut(s) | 177, 755 |
| SmoI | CTYRAG | 2 cut(s) | 177, 755 |
| SnaBI | TACGTA | 2 cut(s) | 688, 1031 |
| SphI | GCATGC | 1 cut(s) | 1018 |
| Sse9I | AATT | 3 cut(s) | 359, 569, 930 |
| SseBI | AGGCCT | 1 cut(s) | 58 |
| SspDI | GGCGCC | 2 cut(s) | 589, 946 |
| SspMI | CTAG | 4 cut(s) | 60, 296, 462, 917 |
| StuI | AGGCCT | 1 cut(s) | 58 |
| StyD4I | CCNGG | 8 cut(s) | 221, 339, 375, 437, 861, 897, 950, 1003 |
| StyI | CCWWGG | 2 cut(s) | 492, 830 |
| TaaI | ACNGT | 3 cut(s) | 132, 161, 235 |
| TaiI | ACGT | 6 cut(s) | 491, 501, 560, 690, 981, 1033 |
| TaqI | TCGA | 6 cut(s) | 103, 230, 305, 348, 454, 581 |
| TasI | AATT | 3 cut(s) | 359, 569, 930 |
| TauI | GCSGC | 3 cut(s) | 213, 383, 947 |
| Tru1I | TTAA | 1 cut(s) | 893 |
| Tru9I | TTAA | 1 cut(s) | 893 |
| TseFI | GTSAC | 4 cut(s) | 38, 553, 771, 1052 |
| TseI | GCWGC | 1 cut(s) | 1064 |
| Tsp45I | GTSAC | 4 cut(s) | 38, 553, 771, 1052 |
| Tth111I | GACNNNGTC | 1 cut(s) | 487 |
| VpaK11BI | GGWCC | 5 cut(s) | 147, 235, 676, 954, 991 |
| XapI | RAATTY | 1 cut(s) | 569 |
| XceI | RCATGY | 1 cut(s) | 1018 |
| XmiI | GTMKAC | 1 cut(s) | 348 |
| XspI | CTAG | 4 cut(s) | 60, 296, 462, 917 |