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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 247 |
| Acc36I | ACCTGC | 2 cut(s) | 247, 394 |
| AciI | CCGC | 7 cut(s) | 15, 67, 74, 88, 145, 191, 502 |
| AclWI | GGATC | 1 cut(s) | 201 |
| AcoI | YGGCCR | 1 cut(s) | 261 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 1 cut(s) | 367 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 73, 256, 368, 456, 546 |
| AjnI | CCWGG | 3 cut(s) | 115, 151, 347 |
| AleI | CACNNNNGTG | 1 cut(s) | 269 |
| AluBI | AGCT | 2 cut(s) | 181, 427 |
| AluI | AGCT | 2 cut(s) | 181, 427 |
| Alw26I | GTCTC | 1 cut(s) | 502 |
| AlwI | GGATC | 1 cut(s) | 201 |
| Ama87I | CYCGRG | 1 cut(s) | 505 |
| AoxI | GGCC | 5 cut(s) | 154, 240, 246, 261, 440 |
| ApeKI | GCWGC | 1 cut(s) | 141 |
| AspA2I | CCTAGG | 1 cut(s) | 545 |
| AspS9I | GGNCC | 4 cut(s) | 247, 406, 415, 440 |
| AsuC2I | CCSGG | 4 cut(s) | 71, 320, 410, 457 |
| AsuHPI | GGTGA | 2 cut(s) | 134, 157 |
| AvaI | CYCGRG | 1 cut(s) | 505 |
| AvaII | GGWCC | 2 cut(s) | 406, 415 |
| AvrII | CCTAGG | 1 cut(s) | 545 |
| BaeGI | GKGCMC | 1 cut(s) | 456 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| BanII | GRGCYC | 1 cut(s) | 231 |
| BbvI | GCAGC | 1 cut(s) | 153 |
| BciT130I | CCWGG | 3 cut(s) | 117, 153, 349 |
| BcnI | CCSGG | 4 cut(s) | 71, 320, 410, 457 |
| BcoDI | GTCTC | 1 cut(s) | 502 |
| BfaI | CTAG | 4 cut(s) | 302, 363, 422, 546 |
| BfuAI | ACCTGC | 2 cut(s) | 247, 394 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 153, 320 |
| BisI | GCNGC | 2 cut(s) | 142, 145 |
| BlnI | CCTAGG | 1 cut(s) | 545 |
| BlsI | GCNGC | 2 cut(s) | 143, 146 |
| BmcAI | AGTACT | 1 cut(s) | 367 |
| Bme1390I | CCNGG | 7 cut(s) | 71, 117, 153, 320, 349, 410, 457 |
| Bme18I | GGWCC | 2 cut(s) | 406, 415 |
| BmeT110I | CYCGRG | 1 cut(s) | 505 |
| BmgT120I | GGNCC | 4 cut(s) | 247, 406, 415, 440 |
| BmiI | GGNNCC | 4 cut(s) | 408, 417, 441, 533 |
| BmrFI | CCNGG | 7 cut(s) | 71, 117, 153, 320, 349, 410, 457 |
| BmrI | ACTGGG | 1 cut(s) | 101 |
| BmuI | ACTGGG | 1 cut(s) | 101 |
| BpuMI | CCSGG | 4 cut(s) | 71, 320, 410, 457 |
| BsaI | GGTCTC | 1 cut(s) | 502 |
| BsaJI | CCNNGG | 6 cut(s) | 115, 243, 455, 512, 525, 545 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 467, 497 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 73, 256, 368, 456, 546 |
| Bse1I | ACTGG | 3 cut(s) | 43, 107, 373 |
| Bse3DI | GCAATG | 1 cut(s) | 488 |
| BseBI | CCWGG | 3 cut(s) | 117, 153, 349 |
| BseDI | CCNNGG | 6 cut(s) | 115, 243, 455, 512, 525, 545 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 73, 256, 368, 456, 546 |
| BseMI | GCAATG | 1 cut(s) | 488 |
| BseNI | ACTGG | 3 cut(s) | 43, 107, 373 |
| BseSI | GKGCMC | 1 cut(s) | 456 |
| BseXI | GCAGC | 1 cut(s) | 153 |
| BshFI | GGCC | 5 cut(s) | 156, 242, 248, 263, 442 |
| BsiHKCI | CYCGRG | 1 cut(s) | 505 |
| BsiSI | CCGG | 4 cut(s) | 71, 320, 410, 457 |
| BslFI | GGGAC | 1 cut(s) | 392 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 73, 256, 368, 456, 546 |
| BsmAI | GTCTC | 1 cut(s) | 502 |
| BsmFI | GGGAC | 1 cut(s) | 392 |
| BsnI | GGCC | 5 cut(s) | 156, 242, 248, 263, 442 |
| Bso31I | GGTCTC | 1 cut(s) | 502 |
| BsoBI | CYCGRG | 1 cut(s) | 505 |
| Bsp1286I | GDGCHC | 2 cut(s) | 231, 456 |
| Bsp143I | GATC | 1 cut(s) | 193 |
| BspACI | CCGC | 7 cut(s) | 15, 67, 74, 88, 145, 191, 502 |
| BspANI | GGCC | 5 cut(s) | 156, 242, 248, 263, 442 |
| BspLI | GGNNCC | 4 cut(s) | 408, 417, 441, 533 |
| BspMI | ACCTGC | 2 cut(s) | 247, 394 |
| BspPI | GGATC | 1 cut(s) | 201 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspTNI | GGTCTC | 1 cut(s) | 502 |
| BsrDI | GCAATG | 1 cut(s) | 488 |
| BsrI | ACTGG | 3 cut(s) | 43, 107, 373 |
| BssECI | CCNNGG | 6 cut(s) | 115, 243, 455, 512, 525, 545 |
| BssMI | GATC | 1 cut(s) | 193 |
| BssT1I | CCWWGG | 2 cut(s) | 512, 545 |
| Bst2UI | CCWGG | 3 cut(s) | 117, 153, 349 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 92, 183, 332 |
| BstDEI | CTNAG | 1 cut(s) | 290 |
| BstKTI | GATC | 1 cut(s) | 196 |
| BstMAI | GTCTC | 1 cut(s) | 502 |
| BstMBI | GATC | 1 cut(s) | 193 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 153, 320, 476 |
| BstNI | CCWGG | 3 cut(s) | 117, 153, 349 |
| BstSCI | CCNGG | 7 cut(s) | 69, 115, 151, 318, 347, 408, 455 |
| BstSLI | GKGCMC | 1 cut(s) | 456 |
| BstV1I | GCAGC | 1 cut(s) | 153 |
| BstX2I | RGATCY | 1 cut(s) | 193 |
| BstYI | RGATCY | 1 cut(s) | 193 |
| BsuRI | GGCC | 5 cut(s) | 156, 242, 248, 263, 442 |
| BtsIMutI | CAGTG | 1 cut(s) | 374 |
| BveI | ACCTGC | 2 cut(s) | 247, 394 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 92, 183, 332 |
| Cfr13I | GGNCC | 4 cut(s) | 247, 406, 415, 440 |
| Csp6I | GTAC | 1 cut(s) | 366 |
| CviAII | CATG | 1 cut(s) | 324 |
| CviQI | GTAC | 1 cut(s) | 366 |
| DdeI | CTNAG | 1 cut(s) | 290 |
| DpnI | GATC | 1 cut(s) | 195 |
| DpnII | GATC | 1 cut(s) | 193 |
| EaeI | YGGCCR | 1 cut(s) | 261 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 1 cut(s) | 206 |
| Eco130I | CCWWGG | 2 cut(s) | 512, 545 |
| Eco24I | GRGCYC | 1 cut(s) | 231 |
| Eco31I | GGTCTC | 1 cut(s) | 502 |
| Eco47I | GGWCC | 2 cut(s) | 406, 415 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| Eco88I | CYCGRG | 1 cut(s) | 505 |
| EcoO109I | RGGNCCY | 1 cut(s) | 406 |
| EcoRII | CCWGG | 3 cut(s) | 115, 151, 347 |
| EcoT14I | CCWWGG | 2 cut(s) | 512, 545 |
| EcoT38I | GRGCYC | 1 cut(s) | 231 |
| ErhI | CCWWGG | 2 cut(s) | 512, 545 |
| FaeI | CATG | 1 cut(s) | 327 |
| FaiI | YATR | 6 cut(s) | 177, 201, 325, 354, 399, 401 |
| FaqI | GGGAC | 1 cut(s) | 392 |
| FatI | CATG | 1 cut(s) | 323 |
| FauI | CCCGC | 2 cut(s) | 8, 74 |
| FauNDI | CATATG | 1 cut(s) | 399 |
| Fnu4HI | GCNGC | 2 cut(s) | 142, 145 |
| FriOI | GRGCYC | 1 cut(s) | 231 |
| Fsp4HI | GCNGC | 2 cut(s) | 142, 145 |
| FspBI | CTAG | 4 cut(s) | 302, 363, 422, 546 |
| GluI | GCNGC | 2 cut(s) | 142, 145 |
| HaeIII | GGCC | 5 cut(s) | 156, 242, 248, 263, 442 |
| HapII | CCGG | 4 cut(s) | 71, 320, 410, 457 |
| Hin1II | CATG | 1 cut(s) | 327 |
| HinfI | GANTC | 1 cut(s) | 482 |
| HpaII | CCGG | 4 cut(s) | 71, 320, 410, 457 |
| HphI | GGTGA | 2 cut(s) | 134, 157 |
| Hpy166II | GTNNAC | 4 cut(s) | 79, 112, 221, 415 |
| Hpy188III | TCNNGA | 1 cut(s) | 339 |
| Hpy8I | GTNNAC | 4 cut(s) | 79, 112, 221, 415 |
| HpyCH4IV | ACGT | 3 cut(s) | 217, 266, 277 |
| HpyCH4V | TGCA | 3 cut(s) | 397, 403, 493 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 153, 320, 476 |
| HpyF3I | CTNAG | 1 cut(s) | 290 |
| HpySE526I | ACGT | 3 cut(s) | 217, 266, 277 |
| Hsp92II | CATG | 1 cut(s) | 327 |
| Kzo9I | GATC | 1 cut(s) | 193 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 327 |
| Lsp1109I | GCAGC | 1 cut(s) | 153 |
| MaeI | CTAG | 4 cut(s) | 302, 363, 422, 546 |
| MaeII | ACGT | 3 cut(s) | 217, 266, 277 |
| MaeIII | GTNAC | 2 cut(s) | 273, 336 |
| MalI | GATC | 1 cut(s) | 195 |
| MboI | GATC | 1 cut(s) | 193 |
| MboII | GAAGA | 3 cut(s) | 41, 188, 306 |
| MflI | RGATCY | 1 cut(s) | 193 |
| MhlI | GDGCHC | 2 cut(s) | 231, 456 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MlyI | GAGTC | 1 cut(s) | 491 |
| MnlI | CCTC | 7 cut(s) | 118, 173, 178, 238, 298, 469, 520 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| MseI | TTAA | 1 cut(s) | 461 |
| MslI | CAYNNNNRTG | 1 cut(s) | 269 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MspI | CCGG | 4 cut(s) | 71, 320, 410, 457 |
| MspR9I | CCNGG | 7 cut(s) | 71, 117, 153, 320, 349, 410, 457 |
| MvaI | CCWGG | 3 cut(s) | 117, 153, 349 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 153, 320, 476 |
| NciI | CCSGG | 4 cut(s) | 71, 320, 410, 457 |
| NdeI | CATATG | 1 cut(s) | 399 |
| NdeII | GATC | 1 cut(s) | 193 |
| NlaIII | CATG | 1 cut(s) | 327 |
| NlaIV | GGNNCC | 4 cut(s) | 408, 417, 441, 533 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 2 cut(s) | 273, 336 |
| OliI | CACNNNNGTG | 1 cut(s) | 269 |
| PaqCI | CACCTGC | 1 cut(s) | 247 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PkrI | GCNGC | 2 cut(s) | 143, 146 |
| PleI | GAGTC | 1 cut(s) | 490 |
| PpsI | GAGTC | 1 cut(s) | 490 |
| PpuMI | RGGWCCY | 1 cut(s) | 406 |
| Psp5II | RGGWCCY | 1 cut(s) | 406 |
| Psp6I | CCWGG | 3 cut(s) | 115, 151, 347 |
| PspGI | CCWGG | 3 cut(s) | 115, 151, 347 |
| PspN4I | GGNNCC | 4 cut(s) | 408, 417, 441, 533 |
| PspPI | GGNCC | 4 cut(s) | 247, 406, 415, 440 |
| PspPPI | RGGWCCY | 1 cut(s) | 406 |
| PsuI | RGATCY | 1 cut(s) | 193 |
| RsaI | GTAC | 1 cut(s) | 367 |
| RsaNI | GTAC | 1 cut(s) | 366 |
| RseI | CAYNNNNRTG | 1 cut(s) | 269 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 461 |
| SatI | GCNGC | 2 cut(s) | 142, 145 |
| Sau3AI | GATC | 1 cut(s) | 193 |
| Sau96I | GGNCC | 4 cut(s) | 247, 406, 415, 440 |
| ScaI | AGTACT | 1 cut(s) | 367 |
| SchI | GAGTC | 1 cut(s) | 491 |
| ScrFI | CCNGG | 7 cut(s) | 71, 117, 153, 320, 349, 410, 457 |
| SduI | GDGCHC | 2 cut(s) | 231, 456 |
| SinI | GGWCC | 2 cut(s) | 406, 415 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 269 |
| SsiI | CCGC | 7 cut(s) | 15, 67, 74, 88, 145, 191, 502 |
| SspMI | CTAG | 4 cut(s) | 302, 363, 422, 546 |
| StyD4I | CCNGG | 7 cut(s) | 69, 115, 151, 318, 347, 408, 455 |
| StyI | CCWWGG | 2 cut(s) | 512, 545 |
| TaiI | ACGT | 3 cut(s) | 220, 269, 280 |
| TatI | WGTACW | 1 cut(s) | 365 |
| TauI | GCSGC | 1 cut(s) | 147 |
| Tru1I | TTAA | 1 cut(s) | 461 |
| Tru9I | TTAA | 1 cut(s) | 461 |
| TscAI | CASTG | 1 cut(s) | 381 |
| TseFI | GTSAC | 2 cut(s) | 273, 336 |
| TseI | GCWGC | 1 cut(s) | 141 |
| Tsp45I | GTSAC | 2 cut(s) | 273, 336 |
| TspDTI | ATGAA | 2 cut(s) | 188, 300 |
| TspRI | CASTG | 1 cut(s) | 381 |
| VpaK11BI | GGWCC | 2 cut(s) | 406, 415 |
| XmaJI | CCTAGG | 1 cut(s) | 545 |
| XspI | CTAG | 4 cut(s) | 302, 363, 422, 546 |
| ZrmI | AGTACT | 1 cut(s) | 367 |