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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 385 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 869 |
| Acc36I | ACCTGC | 2 cut(s) | 385, 532 |
| Acc65I | GGTACC | 2 cut(s) | 28, 544 |
| AccB1I | GGYRCC | 3 cut(s) | 28, 544, 734 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 162 |
| AccBSI | CCGCTC | 3 cut(s) | 276, 642, 691 |
| AccII | CGCG | 2 cut(s) | 133, 478 |
| AclWI | GGATC | 1 cut(s) | 232 |
| AcoI | YGGCCR | 1 cut(s) | 399 |
| AcsI | RAATTY | 1 cut(s) | 416 |
| AcuI | CTGAAG | 3 cut(s) | 134, 191, 449 |
| AcyI | GRCGYC | 2 cut(s) | 735, 830 |
| AfaI | GTAC | 3 cut(s) | 30, 519, 546 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 44, 162, 218, 340, 394, 663, 727 |
| AgsI | TTSAA | 2 cut(s) | 553, 889 |
| AjnI | CCWGG | 3 cut(s) | 83, 775, 791 |
| AluBI | AGCT | 4 cut(s) | 153, 162, 319, 427 |
| AluI | AGCT | 4 cut(s) | 153, 162, 319, 427 |
| Alw21I | GWGCWC | 2 cut(s) | 357, 485 |
| AlwI | GGATC | 1 cut(s) | 232 |
| AoxI | GGCC | 6 cut(s) | 87, 264, 378, 384, 399, 654 |
| ApaI | GGGCCC | 1 cut(s) | 91 |
| ApeKI | GCWGC | 1 cut(s) | 305 |
| ApoI | RAATTY | 1 cut(s) | 416 |
| Asp718I | GGTACC | 2 cut(s) | 28, 544 |
| AspLEI | GCGC | 5 cut(s) | 120, 135, 369, 737, 745 |
| AsuC2I | CCSGG | 2 cut(s) | 92, 722 |
| AsuHPI | GGTGA | 4 cut(s) | 99, 496, 778, 889 |
| AvaII | GGWCC | 7 cut(s) | 209, 221, 298, 331, 524, 578, 779 |
| BaeGI | GKGCMC | 1 cut(s) | 91 |
| BalI | TGGCCA | 1 cut(s) | 401 |
| BanI | GGYRCC | 3 cut(s) | 28, 544, 734 |
| BanII | GRGCYC | 2 cut(s) | 91, 439 |
| BauI | CACGAG | 1 cut(s) | 356 |
| Bbv12I | GWGCWC | 2 cut(s) | 357, 485 |
| BbvI | GCAGC | 1 cut(s) | 292 |
| BccI | CCATC | 3 cut(s) | 224, 509, 815 |
| BciT130I | CCWGG | 3 cut(s) | 85, 777, 793 |
| BciVI | GTATCC | 1 cut(s) | 874 |
| BcnI | CCSGG | 2 cut(s) | 92, 722 |
| BfaI | CTAG | 3 cut(s) | 326, 486, 704 |
| BfoI | RGCGCY | 3 cut(s) | 121, 738, 746 |
| BfuAI | ACCTGC | 2 cut(s) | 385, 532 |
| BfuI | GTATCC | 1 cut(s) | 874 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 74 |
| BisI | GCNGC | 5 cut(s) | 121, 277, 306, 640, 853 |
| BlsI | GCNGC | 5 cut(s) | 122, 278, 307, 641, 854 |
| Bme1390I | CCNGG | 5 cut(s) | 85, 92, 722, 777, 793 |
| Bme18I | GGWCC | 7 cut(s) | 209, 221, 298, 331, 524, 578, 779 |
| BmiI | GGNNCC | 7 cut(s) | 30, 89, 90, 210, 546, 579, 736 |
| BmrFI | CCNGG | 5 cut(s) | 85, 92, 722, 777, 793 |
| BmrI | ACTGGG | 3 cut(s) | 452, 672, 809 |
| BmsI | GCATC | 4 cut(s) | 153, 528, 684, 776 |
| BmuI | ACTGGG | 3 cut(s) | 452, 672, 809 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 27 |
| BpmI | CTGGAG | 2 cut(s) | 626, 707 |
| BpuMI | CCSGG | 2 cut(s) | 92, 722 |
| BsaHI | GRCGYC | 2 cut(s) | 735, 830 |
| BsaJI | CCNNGG | 3 cut(s) | 84, 90, 381 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 605, 635 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 44, 162, 218, 340, 394, 663, 727 |
| Bse1I | ACTGG | 4 cut(s) | 458, 667, 690, 815 |
| Bse3DI | GCAATG | 1 cut(s) | 972 |
| BseBI | CCWGG | 3 cut(s) | 85, 777, 793 |
| BseDI | CCNNGG | 3 cut(s) | 84, 90, 381 |
| BseGI | GGATG | 4 cut(s) | 699, 767, 801, 807 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 44, 162, 218, 340, 394, 663, 727 |
| BseMI | GCAATG | 1 cut(s) | 972 |
| BseNI | ACTGG | 4 cut(s) | 458, 667, 690, 815 |
| BseSI | GKGCMC | 1 cut(s) | 91 |
| BseXI | GCAGC | 1 cut(s) | 292 |
| BseYI | CCCAGC | 3 cut(s) | 162, 267, 739 |
| Bsh1236I | CGCG | 2 cut(s) | 133, 478 |
| BshFI | GGCC | 6 cut(s) | 89, 266, 380, 386, 401, 656 |
| BshNI | GGYRCC | 3 cut(s) | 28, 544, 734 |
| BsiHKAI | GWGCWC | 2 cut(s) | 357, 485 |
| BsiSI | CCGG | 2 cut(s) | 92, 721 |
| BslFI | GGGAC | 3 cut(s) | 180, 591, 680 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 44, 162, 218, 340, 394, 663, 727 |
| BsmFI | GGGAC | 3 cut(s) | 180, 591, 680 |
| BsnI | GGCC | 6 cut(s) | 89, 266, 380, 386, 401, 656 |
| Bsp120I | GGGCCC | 1 cut(s) | 87 |
| Bsp1286I | GDGCHC | 4 cut(s) | 91, 357, 439, 485 |
| Bsp143I | GATC | 2 cut(s) | 104, 237 |
| BspANI | GGCC | 6 cut(s) | 89, 266, 380, 386, 401, 656 |
| BspFNI | CGCG | 2 cut(s) | 133, 478 |
| BspLI | GGNNCC | 7 cut(s) | 30, 89, 90, 210, 546, 579, 736 |
| BspMI | ACCTGC | 2 cut(s) | 385, 532 |
| BspPI | GGATC | 1 cut(s) | 232 |
| BspT107I | GGYRCC | 3 cut(s) | 28, 544, 734 |
| BsrBI | CCGCTC | 3 cut(s) | 276, 642, 691 |
| BsrDI | GCAATG | 1 cut(s) | 972 |
| BsrI | ACTGG | 4 cut(s) | 458, 667, 690, 815 |
| BssECI | CCNNGG | 3 cut(s) | 84, 90, 381 |
| BssMI | GATC | 2 cut(s) | 104, 237 |
| BssNI | GRCGYC | 2 cut(s) | 735, 830 |
| BssSI | CACGAG | 1 cut(s) | 356 |
| Bst2BI | CACGAG | 1 cut(s) | 356 |
| Bst2UI | CCWGG | 3 cut(s) | 85, 777, 793 |
| Bst4CI | ACNGT | 2 cut(s) | 494, 549 |
| Bst6I | CTCTTC | 1 cut(s) | 425 |
| BstACI | GRCGYC | 2 cut(s) | 735, 830 |
| BstC8I | GCNNGC | 4 cut(s) | 75, 321, 733, 822 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 338 |
| BstF5I | GGATG | 4 cut(s) | 699, 767, 801, 807 |
| BstFNI | CGCG | 2 cut(s) | 133, 478 |
| BstH2I | RGCGCY | 3 cut(s) | 121, 738, 746 |
| BstHHI | GCGC | 5 cut(s) | 120, 135, 369, 737, 745 |
| BstKTI | GATC | 2 cut(s) | 107, 240 |
| BstMBI | GATC | 2 cut(s) | 104, 237 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 74, 141, 150, 159, 282, 469, 496 |
| BstNI | CCWGG | 3 cut(s) | 85, 777, 793 |
| BstNSI | RCATGY | 1 cut(s) | 824 |
| BstSCI | CCNGG | 5 cut(s) | 83, 90, 720, 775, 791 |
| BstSLI | GKGCMC | 1 cut(s) | 91 |
| BstUI | CGCG | 2 cut(s) | 133, 478 |
| BstV1I | GCAGC | 1 cut(s) | 292 |
| BstX2I | RGATCY | 1 cut(s) | 237 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 514, 685 |
| BstYI | RGATCY | 1 cut(s) | 237 |
| BsuI | GTATCC | 1 cut(s) | 874 |
| BsuRI | GGCC | 6 cut(s) | 89, 266, 380, 386, 401, 656 |
| BtsCI | GGATG | 4 cut(s) | 699, 767, 801, 807 |
| BtsIMutI | CAGTG | 2 cut(s) | 465, 822 |
| BveI | ACCTGC | 2 cut(s) | 385, 532 |
| Cac8I | GCNNGC | 4 cut(s) | 75, 321, 733, 822 |
| CfoI | GCGC | 5 cut(s) | 120, 135, 369, 737, 745 |
| CseI | GACGC | 2 cut(s) | 838, 929 |
| Csp6I | GTAC | 3 cut(s) | 29, 518, 545 |
| CviAII | CATG | 3 cut(s) | 14, 821, 856 |
| CviQI | GTAC | 3 cut(s) | 29, 518, 545 |
| DinI | GGCGCC | 1 cut(s) | 736 |
| DpnI | GATC | 2 cut(s) | 106, 239 |
| DpnII | GATC | 2 cut(s) | 104, 237 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 869 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 869 |
| EaeI | YGGCCR | 1 cut(s) | 399 |
| Eam1104I | CTCTTC | 1 cut(s) | 425 |
| EarI | CTCTTC | 1 cut(s) | 425 |
| EciI | GGCGGA | 3 cut(s) | 201, 632, 800 |
| Eco24I | GRGCYC | 2 cut(s) | 91, 439 |
| Eco47I | GGWCC | 7 cut(s) | 209, 221, 298, 331, 524, 578, 779 |
| Eco57I | CTGAAG | 3 cut(s) | 134, 191, 449 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 338 |
| EcoO109I | RGGNCCY | 2 cut(s) | 88, 298 |
| EcoRII | CCWGG | 3 cut(s) | 83, 775, 791 |
| EcoT38I | GRGCYC | 2 cut(s) | 91, 439 |
| EgeI | GGCGCC | 1 cut(s) | 736 |
| EheI | GGCGCC | 1 cut(s) | 736 |
| FaeI | CATG | 3 cut(s) | 17, 824, 859 |
| FaqI | GGGAC | 3 cut(s) | 180, 591, 680 |
| FatI | CATG | 3 cut(s) | 13, 820, 855 |
| FauI | CCCGC | 1 cut(s) | 249 |
| FauNDI | CATATG | 1 cut(s) | 876 |
| Fnu4HI | GCNGC | 5 cut(s) | 121, 277, 306, 640, 853 |
| FokI | GGATG | 4 cut(s) | 706, 754, 794, 808 |
| FriOI | GRGCYC | 2 cut(s) | 91, 439 |
| Fsp4HI | GCNGC | 5 cut(s) | 121, 277, 306, 640, 853 |
| FspBI | CTAG | 3 cut(s) | 326, 486, 704 |
| GlaI | GCGC | 5 cut(s) | 119, 134, 368, 736, 744 |
| GluI | GCNGC | 5 cut(s) | 121, 277, 306, 640, 853 |
| GsaI | CCCAGC | 3 cut(s) | 166, 271, 743 |
| GsuI | CTGGAG | 2 cut(s) | 626, 707 |
| HaeII | RGCGCY | 3 cut(s) | 121, 738, 746 |
| HaeIII | GGCC | 6 cut(s) | 89, 266, 380, 386, 401, 656 |
| HapII | CCGG | 2 cut(s) | 92, 721 |
| HgaI | GACGC | 2 cut(s) | 838, 929 |
| HhaI | GCGC | 5 cut(s) | 120, 135, 369, 737, 745 |
| Hin1I | GRCGYC | 2 cut(s) | 735, 830 |
| Hin1II | CATG | 3 cut(s) | 17, 824, 859 |
| Hin6I | GCGC | 5 cut(s) | 118, 133, 367, 735, 743 |
| HinP1I | GCGC | 5 cut(s) | 118, 133, 367, 735, 743 |
| HinfI | GANTC | 1 cut(s) | 620 |
| HpaII | CCGG | 2 cut(s) | 92, 721 |
| HphI | GGTGA | 4 cut(s) | 99, 496, 778, 889 |
| Hpy166II | GTNNAC | 2 cut(s) | 97, 520 |
| Hpy188I | TCNGA | 4 cut(s) | 114, 149, 221, 291 |
| Hpy188III | TCNNGA | 4 cut(s) | 350, 440, 587, 771 |
| Hpy8I | GTNNAC | 2 cut(s) | 97, 520 |
| HpyAV | CCTTC | 3 cut(s) | 344, 895, 937 |
| HpyCH4III | ACNGT | 2 cut(s) | 494, 549 |
| HpyCH4V | TGCA | 6 cut(s) | 463, 541, 697, 767, 802, 820 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 74, 141, 150, 159, 282, 469, 496 |
| Hsp92I | GRCGYC | 2 cut(s) | 735, 830 |
| Hsp92II | CATG | 3 cut(s) | 17, 824, 859 |
| HspAI | GCGC | 5 cut(s) | 118, 133, 367, 735, 743 |
| KasI | GGCGCC | 1 cut(s) | 734 |
| KpnI | GGTACC | 2 cut(s) | 32, 548 |
| Kzo9I | GATC | 2 cut(s) | 104, 237 |
| LmnI | GCTCC | 6 cut(s) | 133, 158, 364, 480, 607, 688 |
| Lsp1109I | GCAGC | 1 cut(s) | 292 |
| LweI | GCATC | 4 cut(s) | 153, 528, 684, 776 |
| MaeI | CTAG | 3 cut(s) | 326, 486, 704 |
| MaeIII | GTNAC | 5 cut(s) | 49, 564, 621, 840, 895 |
| MalI | GATC | 2 cut(s) | 106, 239 |
| MbiI | CCGCTC | 3 cut(s) | 276, 642, 691 |
| MboI | GATC | 2 cut(s) | 104, 237 |
| MboII | GAAGA | 2 cut(s) | 442, 455 |
| MflI | RGATCY | 1 cut(s) | 237 |
| MhlI | GDGCHC | 4 cut(s) | 91, 357, 439, 485 |
| MlsI | TGGCCA | 1 cut(s) | 401 |
| MluCI | AATT | 1 cut(s) | 416 |
| MluNI | TGGCCA | 1 cut(s) | 401 |
| Mly113I | GGCGCC | 1 cut(s) | 735 |
| MlyI | GAGTC | 1 cut(s) | 629 |
| Mox20I | TGGCCA | 1 cut(s) | 401 |
| MscI | TGGCCA | 1 cut(s) | 401 |
| MseI | TTAA | 1 cut(s) | 716 |
| MslI | CAYNNNNRTG | 3 cut(s) | 12, 407, 536 |
| Msp20I | TGGCCA | 1 cut(s) | 401 |
| MspA1I | CMGCKG | 3 cut(s) | 260, 510, 852 |
| MspI | CCGG | 2 cut(s) | 92, 721 |
| MspR9I | CCNGG | 5 cut(s) | 85, 92, 722, 777, 793 |
| MvaI | CCWGG | 3 cut(s) | 85, 777, 793 |
| MvnI | CGCG | 2 cut(s) | 133, 478 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 74, 141, 150, 159, 282, 469, 496 |
| NarI | GGCGCC | 1 cut(s) | 735 |
| NciI | CCSGG | 2 cut(s) | 92, 722 |
| NdeI | CATATG | 1 cut(s) | 876 |
| NdeII | GATC | 2 cut(s) | 104, 237 |
| NlaIII | CATG | 3 cut(s) | 17, 824, 859 |
| NlaIV | GGNNCC | 7 cut(s) | 30, 89, 90, 210, 546, 579, 736 |
| NmeAIII | GCCGAG | 2 cut(s) | 297, 406 |
| NmuCI | GTSAC | 4 cut(s) | 49, 621, 840, 895 |
| NspI | RCATGY | 1 cut(s) | 824 |
| PaeI | GCATGC | 1 cut(s) | 824 |
| PaqCI | CACCTGC | 1 cut(s) | 385 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 162 |
| PkrI | GCNGC | 5 cut(s) | 122, 278, 307, 641, 854 |
| PleI | GAGTC | 1 cut(s) | 628 |
| PluTI | GGCGCC | 1 cut(s) | 738 |
| PpsI | GAGTC | 1 cut(s) | 628 |
| PpuMI | RGGWCCY | 1 cut(s) | 298 |
| Psp5II | RGGWCCY | 1 cut(s) | 298 |
| Psp6I | CCWGG | 3 cut(s) | 83, 775, 791 |
| PspFI | CCCAGC | 3 cut(s) | 162, 267, 739 |
| PspGI | CCWGG | 3 cut(s) | 83, 775, 791 |
| PspN4I | GGNNCC | 7 cut(s) | 30, 89, 90, 210, 546, 579, 736 |
| PspOMI | GGGCCC | 1 cut(s) | 87 |
| PspPPI | RGGWCCY | 1 cut(s) | 298 |
| PsuI | RGATCY | 1 cut(s) | 237 |
| RsaI | GTAC | 3 cut(s) | 30, 519, 546 |
| RsaNI | GTAC | 3 cut(s) | 29, 518, 545 |
| RseI | CAYNNNNRTG | 3 cut(s) | 12, 407, 536 |
| SaqAI | TTAA | 1 cut(s) | 716 |
| SatI | GCNGC | 5 cut(s) | 121, 277, 306, 640, 853 |
| Sau3AI | GATC | 2 cut(s) | 104, 237 |
| SchI | GAGTC | 1 cut(s) | 629 |
| ScrFI | CCNGG | 5 cut(s) | 85, 92, 722, 777, 793 |
| SduI | GDGCHC | 4 cut(s) | 91, 357, 439, 485 |
| SfaNI | GCATC | 4 cut(s) | 153, 528, 684, 776 |
| SfoI | GGCGCC | 1 cut(s) | 736 |
| SinI | GGWCC | 7 cut(s) | 209, 221, 298, 331, 524, 578, 779 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 12, 407, 536 |
| SphI | GCATGC | 1 cut(s) | 824 |
| Sse9I | AATT | 1 cut(s) | 416 |
| SspDI | GGCGCC | 1 cut(s) | 734 |
| SspMI | CTAG | 3 cut(s) | 326, 486, 704 |
| StyD4I | CCNGG | 5 cut(s) | 83, 90, 720, 775, 791 |
| TaaI | ACNGT | 2 cut(s) | 494, 549 |
| TaqI | TCGA | 3 cut(s) | 349, 644, 915 |
| TasI | AATT | 1 cut(s) | 416 |
| TauI | GCSGC | 4 cut(s) | 123, 279, 642, 855 |
| Tru1I | TTAA | 1 cut(s) | 716 |
| Tru9I | TTAA | 1 cut(s) | 716 |
| TscAI | CASTG | 2 cut(s) | 465, 822 |
| TseFI | GTSAC | 4 cut(s) | 49, 621, 840, 895 |
| TseI | GCWGC | 1 cut(s) | 305 |
| Tsp45I | GTSAC | 4 cut(s) | 49, 621, 840, 895 |
| TspGWI | ACGGA | 1 cut(s) | 537 |
| TspRI | CASTG | 2 cut(s) | 465, 822 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 162 |
| VpaK11BI | GGWCC | 7 cut(s) | 209, 221, 298, 331, 524, 578, 779 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 338 |
| XapI | RAATTY | 1 cut(s) | 416 |
| XceI | RCATGY | 1 cut(s) | 824 |
| XspI | CTAG | 3 cut(s) | 326, 486, 704 |