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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 43 |
| Acc36I | ACCTGC | 1 cut(s) | 319 |
| AccB1I | GGYRCC | 1 cut(s) | 584 |
| AccBSI | CCGCTC | 2 cut(s) | 429, 575 |
| AccII | CGCG | 2 cut(s) | 144, 202 |
| AclWI | GGATC | 2 cut(s) | 126, 487 |
| AcoI | YGGCCR | 1 cut(s) | 75 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AcyI | GRCGYC | 1 cut(s) | 593 |
| AfaI | GTAC | 2 cut(s) | 306, 321 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 293, 471, 474, 475, 476, 627 |
| AgsI | TTSAA | 1 cut(s) | 603 |
| AjnI | CCWGG | 5 cut(s) | 272, 286, 470, 497, 625 |
| AleI | CACNNNNGTG | 1 cut(s) | 194 |
| AluBI | AGCT | 4 cut(s) | 106, 214, 352, 656 |
| AluI | AGCT | 4 cut(s) | 106, 214, 352, 656 |
| Alw21I | GWGCWC | 2 cut(s) | 272, 342 |
| Alw26I | GTCTC | 1 cut(s) | 512 |
| Alw44I | GTGCAC | 1 cut(s) | 338 |
| AlwI | GGATC | 2 cut(s) | 126, 487 |
| Ama87I | CYCGRG | 2 cut(s) | 430, 570 |
| AoxI | GGCC | 5 cut(s) | 75, 165, 171, 434, 629 |
| ApaLI | GTGCAC | 1 cut(s) | 338 |
| ApeKI | GCWGC | 2 cut(s) | 547, 641 |
| AspA2I | CCTAGG | 1 cut(s) | 437 |
| AspLEI | GCGC | 2 cut(s) | 144, 204 |
| AspS9I | GGNCC | 4 cut(s) | 172, 331, 365, 629 |
| AsuC2I | CCSGG | 2 cut(s) | 245, 335 |
| AsuHPI | GGTGA | 1 cut(s) | 59 |
| AsuNHI | GCTAGC | 1 cut(s) | 352 |
| AvaI | CYCGRG | 2 cut(s) | 430, 570 |
| AvaII | GGWCC | 2 cut(s) | 331, 365 |
| AvrII | CCTAGG | 1 cut(s) | 437 |
| BaeGI | GKGCMC | 2 cut(s) | 342, 381 |
| BanI | GGYRCC | 1 cut(s) | 584 |
| Bbv12I | GWGCWC | 2 cut(s) | 272, 342 |
| BbvI | GCAGC | 2 cut(s) | 559, 628 |
| BciT130I | CCWGG | 5 cut(s) | 274, 288, 472, 499, 627 |
| BciVI | GTATCC | 1 cut(s) | 487 |
| BcnI | CCSGG | 2 cut(s) | 245, 335 |
| BcoDI | GTCTC | 1 cut(s) | 512 |
| BfaI | CTAG | 3 cut(s) | 63, 353, 438 |
| BfuAI | ACCTGC | 1 cut(s) | 319 |
| BfuI | GTATCC | 1 cut(s) | 487 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 245 |
| BisI | GCNGC | 3 cut(s) | 75, 548, 642 |
| BlnI | CCTAGG | 1 cut(s) | 437 |
| BlsI | GCNGC | 3 cut(s) | 76, 549, 643 |
| Bme1390I | CCNGG | 7 cut(s) | 245, 274, 288, 335, 472, 499, 627 |
| Bme18I | GGWCC | 2 cut(s) | 331, 365 |
| BmeT110I | CYCGRG | 2 cut(s) | 430, 570 |
| BmgT120I | GGNCC | 4 cut(s) | 172, 331, 365, 629 |
| BmiI | GGNNCC | 2 cut(s) | 333, 586 |
| BmrFI | CCNGG | 7 cut(s) | 245, 274, 288, 335, 472, 499, 627 |
| BmsI | GCATC | 2 cut(s) | 508, 534 |
| BmtI | GCTAGC | 1 cut(s) | 356 |
| BpmI | CTGGAG | 1 cut(s) | 248 |
| BpuMI | CCSGG | 2 cut(s) | 245, 335 |
| BsaHI | GRCGYC | 1 cut(s) | 593 |
| BsaI | GGTCTC | 1 cut(s) | 512 |
| BsaJI | CCNNGG | 5 cut(s) | 168, 437, 450, 470, 626 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 288, 318, 392, 422 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 293, 471, 474, 475, 476, 627 |
| Bse118I | RCCGGY | 2 cut(s) | 512, 664 |
| Bse1I | ACTGG | 3 cut(s) | 43, 314, 540 |
| BseBI | CCWGG | 5 cut(s) | 274, 288, 472, 499, 627 |
| BseDI | CCNNGG | 5 cut(s) | 168, 437, 450, 470, 626 |
| BseGI | GGATG | 1 cut(s) | 549 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 293, 471, 474, 475, 476, 627 |
| BseNI | ACTGG | 3 cut(s) | 43, 314, 540 |
| BseSI | GKGCMC | 2 cut(s) | 342, 381 |
| BseXI | GCAGC | 2 cut(s) | 559, 628 |
| BseYI | CCCAGC | 3 cut(s) | 70, 381, 502 |
| BsgI | GTGCAG | 1 cut(s) | 401 |
| Bsh1236I | CGCG | 2 cut(s) | 144, 202 |
| BshFI | GGCC | 5 cut(s) | 77, 167, 173, 436, 631 |
| BshNI | GGYRCC | 1 cut(s) | 584 |
| BsiHKAI | GWGCWC | 2 cut(s) | 272, 342 |
| BsiHKCI | CYCGRG | 2 cut(s) | 430, 570 |
| BsiSI | CCGG | 4 cut(s) | 245, 335, 513, 665 |
| BslFI | GGGAC | 1 cut(s) | 317 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 293, 471, 474, 475, 476, 627 |
| BsmAI | GTCTC | 1 cut(s) | 512 |
| BsmFI | GGGAC | 1 cut(s) | 317 |
| BsnI | GGCC | 5 cut(s) | 77, 167, 173, 436, 631 |
| Bso31I | GGTCTC | 1 cut(s) | 512 |
| BsoBI | CYCGRG | 2 cut(s) | 430, 570 |
| Bsp1286I | GDGCHC | 3 cut(s) | 272, 342, 381 |
| Bsp143I | GATC | 3 cut(s) | 118, 479, 526 |
| BspANI | GGCC | 5 cut(s) | 77, 167, 173, 436, 631 |
| BspFNI | CGCG | 2 cut(s) | 144, 202 |
| BspLI | GGNNCC | 2 cut(s) | 333, 586 |
| BspMI | ACCTGC | 1 cut(s) | 319 |
| BspOI | GCTAGC | 1 cut(s) | 356 |
| BspPI | GGATC | 2 cut(s) | 126, 487 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 584 |
| BspTNI | GGTCTC | 1 cut(s) | 512 |
| BsrBI | CCGCTC | 2 cut(s) | 429, 575 |
| BsrFI | RCCGGY | 2 cut(s) | 512, 664 |
| BsrI | ACTGG | 3 cut(s) | 43, 314, 540 |
| BssAI | RCCGGY | 2 cut(s) | 512, 664 |
| BssECI | CCNNGG | 5 cut(s) | 168, 437, 450, 470, 626 |
| BssMI | GATC | 3 cut(s) | 118, 479, 526 |
| BssNI | GRCGYC | 1 cut(s) | 593 |
| BssT1I | CCWWGG | 1 cut(s) | 437 |
| Bst2UI | CCWGG | 5 cut(s) | 274, 288, 472, 499, 627 |
| Bst4CI | ACNGT | 1 cut(s) | 261 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstACI | GRCGYC | 1 cut(s) | 593 |
| BstC8I | GCNNGC | 5 cut(s) | 108, 146, 354, 514, 654 |
| BstF5I | GGATG | 1 cut(s) | 549 |
| BstFNI | CGCG | 2 cut(s) | 144, 202 |
| BstHHI | GCGC | 2 cut(s) | 144, 204 |
| BstKTI | GATC | 3 cut(s) | 121, 482, 529 |
| BstMAI | GTCTC | 1 cut(s) | 512 |
| BstMBI | GATC | 3 cut(s) | 118, 479, 526 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 199, 245, 376, 518, 556 |
| BstNI | CCWGG | 5 cut(s) | 274, 288, 472, 499, 627 |
| BstNSI | RCATGY | 1 cut(s) | 328 |
| BstSCI | CCNGG | 7 cut(s) | 243, 272, 286, 333, 470, 497, 625 |
| BstSLI | GKGCMC | 2 cut(s) | 342, 381 |
| BstUI | CGCG | 2 cut(s) | 144, 202 |
| BstV1I | GCAGC | 2 cut(s) | 559, 628 |
| BstX2I | RGATCY | 1 cut(s) | 118 |
| BstYI | RGATCY | 1 cut(s) | 118 |
| BsuI | GTATCC | 1 cut(s) | 487 |
| BsuRI | GGCC | 5 cut(s) | 77, 167, 173, 436, 631 |
| BtgZI | GCGATG | 1 cut(s) | 531 |
| BtsCI | GGATG | 1 cut(s) | 549 |
| BtsI | GCAGTG | 2 cut(s) | 413, 636 |
| BtsIMutI | CAGTG | 2 cut(s) | 413, 636 |
| BveI | ACCTGC | 1 cut(s) | 319 |
| Cac8I | GCNNGC | 5 cut(s) | 108, 146, 354, 514, 654 |
| CfoI | GCGC | 2 cut(s) | 144, 204 |
| Cfr10I | RCCGGY | 2 cut(s) | 512, 664 |
| Cfr13I | GGNCC | 4 cut(s) | 172, 331, 365, 629 |
| CseI | GACGC | 1 cut(s) | 601 |
| CsiI | ACCWGGT | 1 cut(s) | 286 |
| Csp6I | GTAC | 2 cut(s) | 305, 320 |
| CviAII | CATG | 1 cut(s) | 325 |
| CviQI | GTAC | 2 cut(s) | 305, 320 |
| DpnI | GATC | 3 cut(s) | 120, 481, 528 |
| DpnII | GATC | 3 cut(s) | 118, 479, 526 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 43 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 43 |
| EaeI | YGGCCR | 1 cut(s) | 75 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 1 cut(s) | 131 |
| Eco130I | CCWWGG | 1 cut(s) | 437 |
| Eco147I | AGGCCT | 1 cut(s) | 436 |
| Eco31I | GGTCTC | 1 cut(s) | 512 |
| Eco47I | GGWCC | 2 cut(s) | 331, 365 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| Eco88I | CYCGRG | 2 cut(s) | 430, 570 |
| EcoO109I | RGGNCCY | 1 cut(s) | 331 |
| EcoRII | CCWGG | 5 cut(s) | 272, 286, 470, 497, 625 |
| EcoT14I | CCWWGG | 1 cut(s) | 437 |
| ErhI | CCWWGG | 1 cut(s) | 437 |
| FaeI | CATG | 1 cut(s) | 328 |
| FaiI | YATR | 5 cut(s) | 100, 102, 279, 326, 670 |
| FaqI | GGGAC | 1 cut(s) | 317 |
| FatI | CATG | 1 cut(s) | 324 |
| FauI | CCCGC | 5 cut(s) | 8, 349, 468, 472, 483 |
| Fnu4HI | GCNGC | 3 cut(s) | 75, 548, 642 |
| FokI | GGATG | 1 cut(s) | 556 |
| Fsp4HI | GCNGC | 3 cut(s) | 75, 548, 642 |
| FspBI | CTAG | 3 cut(s) | 63, 353, 438 |
| GlaI | GCGC | 2 cut(s) | 143, 203 |
| GluI | GCNGC | 3 cut(s) | 75, 548, 642 |
| GsaI | CCCAGC | 3 cut(s) | 74, 385, 506 |
| GsuI | CTGGAG | 1 cut(s) | 248 |
| HaeIII | GGCC | 5 cut(s) | 77, 167, 173, 436, 631 |
| HapII | CCGG | 4 cut(s) | 245, 335, 513, 665 |
| HgaI | GACGC | 1 cut(s) | 601 |
| HhaI | GCGC | 2 cut(s) | 144, 204 |
| Hin1I | GRCGYC | 1 cut(s) | 593 |
| Hin1II | CATG | 1 cut(s) | 328 |
| Hin6I | GCGC | 2 cut(s) | 142, 202 |
| HinP1I | GCGC | 2 cut(s) | 142, 202 |
| HinfI | GANTC | 4 cut(s) | 407, 578, 617, 646 |
| HpaII | CCGG | 4 cut(s) | 245, 335, 513, 665 |
| HphI | GGTGA | 1 cut(s) | 59 |
| Hpy166II | GTNNAC | 2 cut(s) | 322, 340 |
| Hpy188I | TCNGA | 2 cut(s) | 296, 662 |
| Hpy188III | TCNNGA | 1 cut(s) | 621 |
| Hpy8I | GTNNAC | 2 cut(s) | 322, 340 |
| Hpy99I | CGWCG | 1 cut(s) | 595 |
| HpyCH4III | ACNGT | 1 cut(s) | 261 |
| HpyCH4V | TGCA | 6 cut(s) | 328, 340, 418, 521, 547, 652 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 199, 245, 376, 518, 556 |
| Hsp92I | GRCGYC | 1 cut(s) | 593 |
| Hsp92II | CATG | 1 cut(s) | 328 |
| HspAI | GCGC | 2 cut(s) | 142, 202 |
| KroI | GCCGGC | 1 cut(s) | 512 |
| KroNI | GCCGGC | 1 cut(s) | 514 |
| Kzo9I | GATC | 3 cut(s) | 118, 479, 526 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 3 cut(s) | 267, 296, 556 |
| Lsp1109I | GCAGC | 2 cut(s) | 559, 628 |
| LweI | GCATC | 2 cut(s) | 508, 534 |
| MabI | ACCWGGT | 1 cut(s) | 286 |
| MaeI | CTAG | 3 cut(s) | 63, 353, 438 |
| MaeIII | GTNAC | 3 cut(s) | 65, 184, 261 |
| MalI | GATC | 3 cut(s) | 120, 481, 528 |
| MbiI | CCGCTC | 2 cut(s) | 429, 575 |
| MboI | GATC | 3 cut(s) | 118, 479, 526 |
| MboII | GAAGA | 2 cut(s) | 41, 113 |
| MflI | RGATCY | 1 cut(s) | 118 |
| MhlI | GDGCHC | 3 cut(s) | 272, 342, 381 |
| MluCI | AATT | 2 cut(s) | 360, 597 |
| MlyI | GAGTC | 3 cut(s) | 416, 587, 626 |
| MmeI | TCCRAC | 2 cut(s) | 372, 534 |
| MnlI | CCTC | 7 cut(s) | 94, 99, 163, 223, 394, 426, 445 |
| MreI | CGCCGGCG | 1 cut(s) | 512 |
| MroNI | GCCGGC | 1 cut(s) | 512 |
| MslI | CAYNNNNRTG | 1 cut(s) | 194 |
| MspA1I | CMGCKG | 2 cut(s) | 74, 490 |
| MspI | CCGG | 4 cut(s) | 245, 335, 513, 665 |
| MspR9I | CCNGG | 7 cut(s) | 245, 274, 288, 335, 472, 499, 627 |
| MvaI | CCWGG | 5 cut(s) | 274, 288, 472, 499, 627 |
| MvnI | CGCG | 2 cut(s) | 144, 202 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 199, 245, 376, 518, 556 |
| NaeI | GCCGGC | 1 cut(s) | 514 |
| NciI | CCSGG | 2 cut(s) | 245, 335 |
| NdeII | GATC | 3 cut(s) | 118, 479, 526 |
| NgoMIV | GCCGGC | 1 cut(s) | 512 |
| NheI | GCTAGC | 1 cut(s) | 352 |
| NlaIII | CATG | 1 cut(s) | 328 |
| NlaIV | GGNNCC | 2 cut(s) | 333, 586 |
| NmeAIII | GCCGAG | 1 cut(s) | 193 |
| NmuCI | GTSAC | 3 cut(s) | 65, 184, 261 |
| NspI | RCATGY | 1 cut(s) | 328 |
| OliI | CACNNNNGTG | 1 cut(s) | 194 |
| PaeR7I | CTCGAG | 1 cut(s) | 430 |
| PceI | AGGCCT | 1 cut(s) | 436 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PdiI | GCCGGC | 1 cut(s) | 514 |
| PfeI | GAWTC | 1 cut(s) | 646 |
| PkrI | GCNGC | 3 cut(s) | 76, 549, 643 |
| PleI | GAGTC | 3 cut(s) | 415, 586, 625 |
| PpsI | GAGTC | 3 cut(s) | 415, 586, 625 |
| PpuMI | RGGWCCY | 1 cut(s) | 331 |
| Psp5II | RGGWCCY | 1 cut(s) | 331 |
| Psp6I | CCWGG | 5 cut(s) | 272, 286, 470, 497, 625 |
| PspFI | CCCAGC | 3 cut(s) | 70, 381, 502 |
| PspGI | CCWGG | 5 cut(s) | 272, 286, 470, 497, 625 |
| PspN4I | GGNNCC | 2 cut(s) | 333, 586 |
| PspPI | GGNCC | 4 cut(s) | 172, 331, 365, 629 |
| PspPPI | RGGWCCY | 1 cut(s) | 331 |
| PspXI | VCTCGAGB | 1 cut(s) | 430 |
| PsuI | RGATCY | 1 cut(s) | 118 |
| RsaI | GTAC | 2 cut(s) | 306, 321 |
| RsaNI | GTAC | 2 cut(s) | 305, 320 |
| RseI | CAYNNNNRTG | 1 cut(s) | 194 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SatI | GCNGC | 3 cut(s) | 75, 548, 642 |
| Sau3AI | GATC | 3 cut(s) | 118, 479, 526 |
| Sau96I | GGNCC | 4 cut(s) | 172, 331, 365, 629 |
| SchI | GAGTC | 3 cut(s) | 416, 587, 626 |
| ScrFI | CCNGG | 7 cut(s) | 245, 274, 288, 335, 472, 499, 627 |
| SduI | GDGCHC | 3 cut(s) | 272, 342, 381 |
| SetI | ASST | 9 cut(s) | 64, 86, 108, 216, 289, 333, 354, 500, 658 |
| SexAI | ACCWGGT | 1 cut(s) | 286 |
| SfaNI | GCATC | 2 cut(s) | 508, 534 |
| Sfr274I | CTCGAG | 1 cut(s) | 430 |
| SgrAI | CRCCGGYG | 1 cut(s) | 512 |
| SinI | GGWCC | 2 cut(s) | 331, 365 |
| SlaI | CTCGAG | 1 cut(s) | 430 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 194 |
| SmlI | CTYRAG | 1 cut(s) | 430 |
| SmoI | CTYRAG | 1 cut(s) | 430 |
| Sse9I | AATT | 2 cut(s) | 360, 597 |
| SseBI | AGGCCT | 1 cut(s) | 436 |
| SspMI | CTAG | 3 cut(s) | 63, 353, 438 |
| StuI | AGGCCT | 1 cut(s) | 436 |
| StyD4I | CCNGG | 7 cut(s) | 243, 272, 286, 333, 470, 497, 625 |
| StyI | CCWWGG | 1 cut(s) | 437 |
| TaaI | ACNGT | 1 cut(s) | 261 |
| TaqI | TCGA | 1 cut(s) | 431 |
| TasI | AATT | 2 cut(s) | 360, 597 |
| TauI | GCSGC | 1 cut(s) | 77 |
| TfiI | GAWTC | 1 cut(s) | 646 |
| TscAI | CASTG | 2 cut(s) | 420, 643 |
| TseFI | GTSAC | 3 cut(s) | 65, 184, 261 |
| TseI | GCWGC | 2 cut(s) | 547, 641 |
| Tsp45I | GTSAC | 3 cut(s) | 65, 184, 261 |
| TspGWI | ACGGA | 2 cut(s) | 280, 556 |
| TspRI | CASTG | 2 cut(s) | 420, 643 |
| VneI | GTGCAC | 1 cut(s) | 338 |
| VpaK11BI | GGWCC | 2 cut(s) | 331, 365 |
| XceI | RCATGY | 1 cut(s) | 328 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 468 |
| XhoI | CTCGAG | 1 cut(s) | 430 |
| XmaJI | CCTAGG | 1 cut(s) | 437 |
| XspI | CTAG | 3 cut(s) | 63, 353, 438 |