No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 514 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 680 |
| AatII | GACGTC | 1 cut(s) | 735 |
| Acc36I | ACCTGC | 1 cut(s) | 514 |
| AccII | CGCG | 1 cut(s) | 74 |
| AclWI | GGATC | 1 cut(s) | 48 |
| AcoI | YGGCCR | 2 cut(s) | 209, 324 |
| AcsI | RAATTY | 1 cut(s) | 341 |
| AcyI | GRCGYC | 1 cut(s) | 732 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 139, 140, 265, 301, 431, 516, 517, 577 |
| AgeI | ACCGGT | 1 cut(s) | 214 |
| AgsI | TTSAA | 2 cut(s) | 118, 700 |
| AjnI | CCWGG | 5 cut(s) | 146, 159, 512, 540, 576 |
| AleI | CACNNNNGTG | 1 cut(s) | 660 |
| AluBI | AGCT | 3 cut(s) | 34, 457, 479 |
| AluI | AGCT | 3 cut(s) | 34, 457, 479 |
| Alw21I | GWGCWC | 2 cut(s) | 112, 410 |
| Alw26I | GTCTC | 2 cut(s) | 182, 552 |
| Alw44I | GTGCAC | 1 cut(s) | 108 |
| AlwI | GGATC | 1 cut(s) | 48 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 166, 551 |
| AoxI | GGCC | 6 cut(s) | 143, 209, 309, 324, 483, 548 |
| ApaI | GGGCCC | 1 cut(s) | 147 |
| ApaLI | GTGCAC | 1 cut(s) | 108 |
| ApeKI | GCWGC | 1 cut(s) | 182 |
| ApoI | RAATTY | 1 cut(s) | 341 |
| AseI | ATTAAT | 1 cut(s) | 96 |
| AsiGI | ACCGGT | 1 cut(s) | 214 |
| AspS9I | GGNCC | 9 cut(s) | 143, 144, 163, 217, 223, 256, 310, 483, 548 |
| AsuC2I | CCSGG | 2 cut(s) | 221, 614 |
| AsuHPI | GGTGA | 1 cut(s) | 727 |
| AsuNHI | GCTAGC | 1 cut(s) | 34 |
| AvaII | GGWCC | 4 cut(s) | 163, 217, 223, 256 |
| BaeGI | GKGCMC | 2 cut(s) | 112, 147 |
| BalI | TGGCCA | 1 cut(s) | 326 |
| BanII | GRGCYC | 2 cut(s) | 147, 155 |
| Bbv12I | GWGCWC | 2 cut(s) | 112, 410 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 1 cut(s) | 194 |
| BccI | CCATC | 2 cut(s) | 66, 238 |
| BciT130I | CCWGG | 5 cut(s) | 148, 161, 514, 542, 578 |
| BcnI | CCSGG | 2 cut(s) | 221, 614 |
| BcoDI | GTCTC | 2 cut(s) | 182, 552 |
| BfaI | CTAG | 6 cut(s) | 35, 135, 365, 480, 608, 764 |
| BfmI | CTRYAG | 1 cut(s) | 658 |
| BfuAI | ACCTGC | 1 cut(s) | 514 |
| BisI | GCNGC | 3 cut(s) | 75, 183, 231 |
| BlsI | GCNGC | 3 cut(s) | 76, 184, 232 |
| Bme1390I | CCNGG | 7 cut(s) | 148, 161, 221, 514, 542, 578, 614 |
| Bme18I | GGWCC | 4 cut(s) | 163, 217, 223, 256 |
| BmgT120I | GGNCC | 9 cut(s) | 143, 144, 163, 217, 223, 256, 310, 483, 548 |
| BmiI | GGNNCC | 3 cut(s) | 144, 145, 224 |
| BmrFI | CCNGG | 7 cut(s) | 148, 161, 221, 514, 542, 578, 614 |
| BmrI | ACTGGG | 1 cut(s) | 206 |
| BmsI | GCATC | 1 cut(s) | 576 |
| BmtI | GCTAGC | 1 cut(s) | 38 |
| BmuI | ACTGGG | 1 cut(s) | 206 |
| BpmI | CTGGAG | 3 cut(s) | 108, 524, 576 |
| Bpu10I | CCTNAGC | 2 cut(s) | 27, 528 |
| BpuMI | CCSGG | 2 cut(s) | 221, 614 |
| BsaHI | GRCGYC | 1 cut(s) | 732 |
| BsaI | GGTCTC | 1 cut(s) | 552 |
| BsaJI | CCNNGG | 5 cut(s) | 72, 146, 147, 159, 512 |
| BsaWI | WCCGGW | 1 cut(s) | 214 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 139, 140, 265, 301, 431, 516, 517, 577 |
| Bse118I | RCCGGY | 1 cut(s) | 214 |
| Bse1I | ACTGG | 2 cut(s) | 201, 436 |
| Bse3DI | GCAATG | 1 cut(s) | 783 |
| BseBI | CCWGG | 5 cut(s) | 148, 161, 514, 542, 578 |
| BseDI | CCNNGG | 5 cut(s) | 72, 146, 147, 159, 512 |
| BseGI | GGATG | 4 cut(s) | 58, 155, 499, 591 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 139, 140, 265, 301, 431, 516, 517, 577 |
| BseMI | GCAATG | 1 cut(s) | 783 |
| BseMII | CTCAG | 2 cut(s) | 18, 542 |
| BseNI | ACTGG | 2 cut(s) | 201, 436 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseSI | GKGCMC | 2 cut(s) | 112, 147 |
| BseXI | GCAGC | 1 cut(s) | 194 |
| BseYI | CCCAGC | 1 cut(s) | 88 |
| Bsh1236I | CGCG | 1 cut(s) | 74 |
| BshFI | GGCC | 6 cut(s) | 145, 211, 311, 326, 485, 550 |
| BshTI | ACCGGT | 1 cut(s) | 214 |
| BsiHKAI | GWGCWC | 2 cut(s) | 112, 410 |
| BsiSI | CCGG | 3 cut(s) | 215, 220, 613 |
| BslFI | GGGAC | 2 cut(s) | 33, 236 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 139, 140, 265, 301, 431, 516, 517, 577 |
| BsmAI | GTCTC | 2 cut(s) | 182, 552 |
| BsmFI | GGGAC | 2 cut(s) | 33, 236 |
| BsnI | GGCC | 6 cut(s) | 145, 211, 311, 326, 485, 550 |
| Bso31I | GGTCTC | 1 cut(s) | 552 |
| Bsp120I | GGGCCC | 1 cut(s) | 143 |
| Bsp1286I | GDGCHC | 4 cut(s) | 112, 147, 155, 410 |
| Bsp143I | GATC | 1 cut(s) | 40 |
| BspANI | GGCC | 6 cut(s) | 145, 211, 311, 326, 485, 550 |
| BspCNI | CTCAG | 2 cut(s) | 19, 541 |
| BspFNI | CGCG | 1 cut(s) | 74 |
| BspLI | GGNNCC | 3 cut(s) | 144, 145, 224 |
| BspMI | ACCTGC | 1 cut(s) | 514 |
| BspOI | GCTAGC | 1 cut(s) | 38 |
| BspPI | GGATC | 1 cut(s) | 48 |
| BspTNI | GGTCTC | 1 cut(s) | 552 |
| BsrDI | GCAATG | 1 cut(s) | 783 |
| BsrFI | RCCGGY | 1 cut(s) | 214 |
| BsrI | ACTGG | 2 cut(s) | 201, 436 |
| BssAI | RCCGGY | 1 cut(s) | 214 |
| BssECI | CCNNGG | 5 cut(s) | 72, 146, 147, 159, 512 |
| BssMI | GATC | 1 cut(s) | 40 |
| BssNI | GRCGYC | 1 cut(s) | 732 |
| Bst2UI | CCWGG | 5 cut(s) | 148, 161, 514, 542, 578 |
| Bst4CI | ACNGT | 4 cut(s) | 11, 107, 662, 770 |
| BstACI | GRCGYC | 1 cut(s) | 732 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 551 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 395, 552 |
| BstDEI | CTNAG | 2 cut(s) | 27, 528 |
| BstDSI | CCRYGG | 1 cut(s) | 72 |
| BstF5I | GGATG | 4 cut(s) | 58, 155, 499, 591 |
| BstFNI | CGCG | 1 cut(s) | 74 |
| BstKTI | GATC | 1 cut(s) | 43 |
| BstMAI | GTCTC | 2 cut(s) | 182, 552 |
| BstMBI | GATC | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 368, 414, 551 |
| BstNI | CCWGG | 5 cut(s) | 148, 161, 514, 542, 578 |
| BstSCI | CCNGG | 7 cut(s) | 146, 159, 219, 512, 540, 576, 612 |
| BstSFI | CTRYAG | 1 cut(s) | 658 |
| BstSLI | GKGCMC | 2 cut(s) | 112, 147 |
| BstUI | CGCG | 1 cut(s) | 74 |
| BstV1I | GCAGC | 1 cut(s) | 194 |
| BsuRI | GGCC | 6 cut(s) | 145, 211, 311, 326, 485, 550 |
| BtgI | CCRYGG | 1 cut(s) | 72 |
| BtsCI | GGATG | 4 cut(s) | 58, 155, 499, 591 |
| BtsIMutI | CAGTG | 3 cut(s) | 329, 667, 775 |
| BveI | ACCTGC | 1 cut(s) | 514 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 395, 552 |
| CaiI | CAGNNNCTG | 2 cut(s) | 166, 551 |
| Cfr10I | RCCGGY | 1 cut(s) | 214 |
| Cfr13I | GGNCC | 9 cut(s) | 143, 144, 163, 217, 223, 256, 310, 483, 548 |
| Cfr42I | CCGCGG | 1 cut(s) | 75 |
| CpoI | CGGWCCG | 1 cut(s) | 217 |
| CseI | GACGC | 1 cut(s) | 423 |
| CspAI | ACCGGT | 1 cut(s) | 214 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 415, 450 |
| CspI | CGGWCCG | 1 cut(s) | 217 |
| CviAII | CATG | 2 cut(s) | 139, 688 |
| DdeI | CTNAG | 2 cut(s) | 27, 528 |
| DpnI | GATC | 1 cut(s) | 42 |
| DpnII | GATC | 1 cut(s) | 40 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 680 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 680 |
| EaeI | YGGCCR | 2 cut(s) | 209, 324 |
| EciI | GGCGGA | 3 cut(s) | 269, 320, 731 |
| Eco24I | GRGCYC | 2 cut(s) | 147, 155 |
| Eco31I | GGTCTC | 1 cut(s) | 552 |
| Eco47I | GGWCC | 4 cut(s) | 163, 217, 223, 256 |
| EcoO109I | RGGNCCY | 4 cut(s) | 143, 163, 223, 548 |
| EcoRII | CCWGG | 5 cut(s) | 146, 159, 512, 540, 576 |
| EcoT38I | GRGCYC | 2 cut(s) | 147, 155 |
| FaeI | CATG | 2 cut(s) | 142, 691 |
| FaiI | YATR | 5 cut(s) | 140, 236, 240, 668, 689 |
| FaqI | GGGAC | 2 cut(s) | 33, 236 |
| FatI | CATG | 2 cut(s) | 138, 687 |
| FauI | CCCGC | 3 cut(s) | 8, 79, 510 |
| Fnu4HI | GCNGC | 3 cut(s) | 75, 183, 231 |
| FokI | GGATG | 4 cut(s) | 45, 142, 486, 598 |
| FriOI | GRGCYC | 2 cut(s) | 147, 155 |
| Fsp4HI | GCNGC | 3 cut(s) | 75, 183, 231 |
| FspBI | CTAG | 6 cut(s) | 35, 135, 365, 480, 608, 764 |
| GluI | GCNGC | 3 cut(s) | 75, 183, 231 |
| GsaI | CCCAGC | 1 cut(s) | 92 |
| GsuI | CTGGAG | 3 cut(s) | 108, 524, 576 |
| HaeIII | GGCC | 6 cut(s) | 145, 211, 311, 326, 485, 550 |
| HapII | CCGG | 3 cut(s) | 215, 220, 613 |
| HgaI | GACGC | 1 cut(s) | 423 |
| Hin1I | GRCGYC | 1 cut(s) | 732 |
| Hin1II | CATG | 2 cut(s) | 142, 691 |
| HincII | GTYRAC | 2 cut(s) | 103, 422 |
| HindII | GTYRAC | 2 cut(s) | 103, 422 |
| HinfI | GANTC | 3 cut(s) | 190, 449, 723 |
| HpaI | GTTAAC | 1 cut(s) | 103 |
| HpaII | CCGG | 3 cut(s) | 215, 220, 613 |
| HphI | GGTGA | 1 cut(s) | 727 |
| Hpy166II | GTNNAC | 4 cut(s) | 103, 110, 422, 685 |
| Hpy188I | TCNGA | 1 cut(s) | 623 |
| Hpy188III | TCNNGA | 1 cut(s) | 166 |
| Hpy8I | GTNNAC | 4 cut(s) | 103, 110, 422, 685 |
| Hpy99I | CGWCG | 2 cut(s) | 731, 734 |
| HpyAV | CCTTC | 3 cut(s) | 295, 688, 706 |
| HpyCH4III | ACNGT | 4 cut(s) | 11, 107, 662, 770 |
| HpyCH4IV | ACGT | 1 cut(s) | 732 |
| HpyCH4V | TGCA | 2 cut(s) | 110, 589 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 368, 414, 551 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 528 |
| HpySE526I | ACGT | 1 cut(s) | 732 |
| Hsp92I | GRCGYC | 1 cut(s) | 732 |
| Hsp92II | CATG | 2 cut(s) | 142, 691 |
| KspAI | GTTAAC | 1 cut(s) | 103 |
| KspI | CCGCGG | 1 cut(s) | 75 |
| Kzo9I | GATC | 1 cut(s) | 40 |
| LmnI | GCTCC | 2 cut(s) | 31, 405 |
| Lsp1109I | GCAGC | 1 cut(s) | 194 |
| LweI | GCATC | 1 cut(s) | 576 |
| MaeI | CTAG | 6 cut(s) | 35, 135, 365, 480, 608, 764 |
| MaeII | ACGT | 1 cut(s) | 732 |
| MaeIII | GTNAC | 3 cut(s) | 651, 706, 733 |
| MalI | GATC | 1 cut(s) | 42 |
| MboI | GATC | 1 cut(s) | 40 |
| MfeI | CAATTG | 2 cut(s) | 398, 466 |
| MhlI | GDGCHC | 4 cut(s) | 112, 147, 155, 410 |
| MlsI | TGGCCA | 1 cut(s) | 326 |
| MluCI | AATT | 5 cut(s) | 341, 350, 398, 466, 593 |
| MluNI | TGGCCA | 1 cut(s) | 326 |
| MlyI | GAGTC | 1 cut(s) | 184 |
| MnlI | CCTC | 9 cut(s) | 22, 236, 269, 301, 361, 436, 548, 749, 766 |
| Mox20I | TGGCCA | 1 cut(s) | 326 |
| MscI | TGGCCA | 1 cut(s) | 326 |
| MseI | TTAA | 4 cut(s) | 84, 96, 102, 353 |
| MslI | CAYNNNNRTG | 2 cut(s) | 332, 660 |
| Msp20I | TGGCCA | 1 cut(s) | 326 |
| MspA1I | CMGCKG | 1 cut(s) | 74 |
| MspI | CCGG | 3 cut(s) | 215, 220, 613 |
| MspR9I | CCNGG | 7 cut(s) | 148, 161, 221, 514, 542, 578, 614 |
| MunI | CAATTG | 2 cut(s) | 398, 466 |
| MvaI | CCWGG | 5 cut(s) | 148, 161, 514, 542, 578 |
| MvnI | CGCG | 1 cut(s) | 74 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 368, 414, 551 |
| NciI | CCSGG | 2 cut(s) | 221, 614 |
| NdeII | GATC | 1 cut(s) | 40 |
| NheI | GCTAGC | 1 cut(s) | 34 |
| NlaIII | CATG | 2 cut(s) | 142, 691 |
| NlaIV | GGNNCC | 3 cut(s) | 144, 145, 224 |
| NmuCI | GTSAC | 3 cut(s) | 651, 706, 733 |
| OliI | CACNNNNGTG | 1 cut(s) | 660 |
| PaqCI | CACCTGC | 1 cut(s) | 514 |
| PasI | CCCWGGG | 1 cut(s) | 147 |
| PfeI | GAWTC | 2 cut(s) | 449, 723 |
| PflFI | GACNNNGTC | 2 cut(s) | 417, 731 |
| PfoI | TCCNGGA | 2 cut(s) | 219, 576 |
| PinAI | ACCGGT | 1 cut(s) | 214 |
| PkrI | GCNGC | 3 cut(s) | 76, 184, 232 |
| PleI | GAGTC | 1 cut(s) | 184 |
| PpsI | GAGTC | 1 cut(s) | 184 |
| PpuMI | RGGWCCY | 2 cut(s) | 163, 223 |
| PshBI | ATTAAT | 1 cut(s) | 96 |
| Psp5II | RGGWCCY | 2 cut(s) | 163, 223 |
| Psp6I | CCWGG | 5 cut(s) | 146, 159, 512, 540, 576 |
| PspFI | CCCAGC | 1 cut(s) | 88 |
| PspGI | CCWGG | 5 cut(s) | 146, 159, 512, 540, 576 |
| PspN4I | GGNNCC | 3 cut(s) | 144, 145, 224 |
| PspOMI | GGGCCC | 1 cut(s) | 143 |
| PspPI | GGNCC | 9 cut(s) | 143, 144, 163, 217, 223, 256, 310, 483, 548 |
| PspPPI | RGGWCCY | 2 cut(s) | 163, 223 |
| PstNI | CAGNNNCTG | 2 cut(s) | 166, 551 |
| PsyI | GACNNNGTC | 2 cut(s) | 417, 731 |
| RseI | CAYNNNNRTG | 2 cut(s) | 332, 660 |
| Rsr2I | CGGWCCG | 1 cut(s) | 217 |
| RsrII | CGGWCCG | 1 cut(s) | 217 |
| SacII | CCGCGG | 1 cut(s) | 75 |
| SaqAI | TTAA | 4 cut(s) | 84, 96, 102, 353 |
| SatI | GCNGC | 3 cut(s) | 75, 183, 231 |
| Sau3AI | GATC | 1 cut(s) | 40 |
| Sau96I | GGNCC | 9 cut(s) | 143, 144, 163, 217, 223, 256, 310, 483, 548 |
| SchI | GAGTC | 1 cut(s) | 184 |
| ScrFI | CCNGG | 7 cut(s) | 148, 161, 221, 514, 542, 578, 614 |
| SduI | GDGCHC | 4 cut(s) | 112, 147, 155, 410 |
| SfaNI | GCATC | 1 cut(s) | 576 |
| SfcI | CTRYAG | 1 cut(s) | 658 |
| Sfr303I | CCGCGG | 1 cut(s) | 75 |
| SgrBI | CCGCGG | 1 cut(s) | 75 |
| SinI | GGWCC | 4 cut(s) | 163, 217, 223, 256 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 332, 660 |
| Sse9I | AATT | 5 cut(s) | 341, 350, 398, 466, 593 |
| SspMI | CTAG | 6 cut(s) | 35, 135, 365, 480, 608, 764 |
| StyD4I | CCNGG | 7 cut(s) | 146, 159, 219, 512, 540, 576, 612 |
| TaaI | ACNGT | 4 cut(s) | 11, 107, 662, 770 |
| TaiI | ACGT | 1 cut(s) | 735 |
| TaqI | TCGA | 2 cut(s) | 345, 726 |
| TasI | AATT | 5 cut(s) | 341, 350, 398, 466, 593 |
| TauI | GCSGC | 2 cut(s) | 77, 233 |
| TfiI | GAWTC | 2 cut(s) | 449, 723 |
| Tru1I | TTAA | 4 cut(s) | 84, 96, 102, 353 |
| Tru9I | TTAA | 4 cut(s) | 84, 96, 102, 353 |
| TscAI | CASTG | 3 cut(s) | 336, 667, 775 |
| TseFI | GTSAC | 3 cut(s) | 651, 706, 733 |
| TseI | GCWGC | 1 cut(s) | 182 |
| Tsp45I | GTSAC | 3 cut(s) | 651, 706, 733 |
| TspDTI | ATGAA | 1 cut(s) | 363 |
| TspRI | CASTG | 3 cut(s) | 336, 667, 775 |
| Tth111I | GACNNNGTC | 2 cut(s) | 417, 731 |
| VneI | GTGCAC | 1 cut(s) | 108 |
| VpaK11BI | GGWCC | 4 cut(s) | 163, 217, 223, 256 |
| VspI | ATTAAT | 1 cut(s) | 96 |
| XapI | RAATTY | 1 cut(s) | 341 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 520 |
| XspI | CTAG | 6 cut(s) | 35, 135, 365, 480, 608, 764 |
| ZraI | GACGTC | 1 cut(s) | 733 |