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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 144 |
| AccIII | TCCGGA | 1 cut(s) | 82 |
| AciI | CCGC | 2 cut(s) | 48, 116 |
| AcoI | YGGCCR | 1 cut(s) | 186 |
| AcsI | RAATTY | 1 cut(s) | 203 |
| AcuI | CTGAAG | 1 cut(s) | 236 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 127 |
| AluBI | AGCT | 3 cut(s) | 106, 214, 248 |
| AluI | AGCT | 3 cut(s) | 106, 214, 248 |
| Aor13HI | TCCGGA | 1 cut(s) | 82 |
| AoxI | GGCC | 2 cut(s) | 171, 186 |
| ApoI | RAATTY | 1 cut(s) | 203 |
| AspLEI | GCGC | 2 cut(s) | 156, 226 |
| AspS9I | GGNCC | 4 cut(s) | 40, 85, 118, 172 |
| AsuHPI | GGTGA | 1 cut(s) | 82 |
| AvaII | GGWCC | 3 cut(s) | 40, 85, 118 |
| BalI | TGGCCA | 1 cut(s) | 188 |
| BccI | CCATC | 2 cut(s) | 66, 71 |
| BfoI | RGCGCY | 1 cut(s) | 227 |
| Bme18I | GGWCC | 3 cut(s) | 40, 85, 118 |
| BmgT120I | GGNCC | 4 cut(s) | 40, 85, 118, 172 |
| BpuEI | CTTGAG | 1 cut(s) | 248 |
| BsaWI | WCCGGW | 1 cut(s) | 82 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 64, 94 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 127 |
| BseAI | TCCGGA | 1 cut(s) | 82 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 127 |
| BseYI | CCCAGC | 1 cut(s) | 244 |
| Bsh1236I | CGCG | 1 cut(s) | 144 |
| BshFI | GGCC | 2 cut(s) | 173, 188 |
| BsiSI | CCGG | 1 cut(s) | 83 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 127 |
| BsnI | GGCC | 2 cut(s) | 173, 188 |
| Bsp13I | TCCGGA | 1 cut(s) | 82 |
| BspACI | CCGC | 2 cut(s) | 48, 116 |
| BspANI | GGCC | 2 cut(s) | 173, 188 |
| BspEI | TCCGGA | 1 cut(s) | 82 |
| BspFNI | CGCG | 1 cut(s) | 144 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstC8I | GCNNGC | 1 cut(s) | 108 |
| BstDEI | CTNAG | 1 cut(s) | 249 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 125 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstFNI | CGCG | 1 cut(s) | 144 |
| BstH2I | RGCGCY | 1 cut(s) | 227 |
| BstHHI | GCGC | 2 cut(s) | 156, 226 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 230, 245 |
| BstUI | CGCG | 1 cut(s) | 144 |
| BsuRI | GGCC | 2 cut(s) | 173, 188 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| Cac8I | GCNNGC | 1 cut(s) | 108 |
| CfoI | GCGC | 2 cut(s) | 156, 226 |
| Cfr13I | GGNCC | 4 cut(s) | 40, 85, 118, 172 |
| CviJI | RGCY | 5 cut(s) | 106, 173, 188, 214, 248 |
| CviKI_1 | RGCY | 5 cut(s) | 106, 173, 188, 214, 248 |
| DdeI | CTNAG | 1 cut(s) | 249 |
| EaeI | YGGCCR | 1 cut(s) | 186 |
| EciI | GGCGGA | 2 cut(s) | 37, 131 |
| Eco47I | GGWCC | 3 cut(s) | 40, 85, 118 |
| Eco57I | CTGAAG | 1 cut(s) | 236 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 125 |
| FaiI | YATR | 2 cut(s) | 98, 102 |
| FokI | GGATG | 1 cut(s) | 45 |
| GlaI | GCGC | 2 cut(s) | 155, 225 |
| GsaI | CCCAGC | 1 cut(s) | 248 |
| HaeII | RGCGCY | 1 cut(s) | 227 |
| HaeIII | GGCC | 2 cut(s) | 173, 188 |
| HapII | CCGG | 1 cut(s) | 83 |
| HhaI | GCGC | 2 cut(s) | 156, 226 |
| Hin6I | GCGC | 2 cut(s) | 154, 224 |
| HinP1I | GCGC | 2 cut(s) | 154, 224 |
| HpaII | CCGG | 1 cut(s) | 83 |
| HphI | GGTGA | 1 cut(s) | 82 |
| Hpy166II | GTNNAC | 1 cut(s) | 40 |
| Hpy188III | TCNNGA | 1 cut(s) | 83 |
| Hpy8I | GTNNAC | 1 cut(s) | 40 |
| Hpy99I | CGWCG | 1 cut(s) | 212 |
| HpyAV | CCTTC | 2 cut(s) | 157, 214 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 230, 245 |
| HpyF3I | CTNAG | 1 cut(s) | 249 |
| HspAI | GCGC | 2 cut(s) | 154, 224 |
| Kpn2I | TCCGGA | 1 cut(s) | 82 |
| LpnPI | CCDG | 4 cut(s) | 20, 92, 96, 121 |
| MboII | GAAGA | 2 cut(s) | 41, 229 |
| MlsI | TGGCCA | 1 cut(s) | 188 |
| MluCI | AATT | 1 cut(s) | 203 |
| MluNI | TGGCCA | 1 cut(s) | 188 |
| MnlI | CCTC | 6 cut(s) | 8, 98, 103, 131, 163, 223 |
| Mox20I | TGGCCA | 1 cut(s) | 188 |
| MroI | TCCGGA | 1 cut(s) | 82 |
| MscI | TGGCCA | 1 cut(s) | 188 |
| MslI | CAYNNNNRTG | 2 cut(s) | 95, 194 |
| Msp20I | TGGCCA | 1 cut(s) | 188 |
| MspI | CCGG | 1 cut(s) | 83 |
| MvnI | CGCG | 1 cut(s) | 144 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 230, 245 |
| PspFI | CCCAGC | 1 cut(s) | 244 |
| PspPI | GGNCC | 4 cut(s) | 40, 85, 118, 172 |
| RseI | CAYNNNNRTG | 2 cut(s) | 95, 194 |
| Sau96I | GGNCC | 4 cut(s) | 40, 85, 118, 172 |
| SetI | ASST | 8 cut(s) | 39, 90, 95, 108, 123, 168, 216, 250 |
| SinI | GGWCC | 3 cut(s) | 40, 85, 118 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 95, 194 |
| SmlI | CTYRAG | 1 cut(s) | 227 |
| SmoI | CTYRAG | 1 cut(s) | 227 |
| Sse9I | AATT | 1 cut(s) | 203 |
| SsiI | CCGC | 2 cut(s) | 48, 116 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaqI | TCGA | 2 cut(s) | 168, 207 |
| TasI | AATT | 1 cut(s) | 203 |
| TspDTI | ATGAA | 1 cut(s) | 42 |
| VpaK11BI | GGWCC | 3 cut(s) | 40, 85, 118 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 125 |
| XapI | RAATTY | 1 cut(s) | 203 |