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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 145 |
| Acc65I | GGTACC | 2 cut(s) | 130, 157 |
| AccB1I | GGYRCC | 3 cut(s) | 130, 157, 410 |
| AciI | CCGC | 3 cut(s) | 161, 208, 465 |
| AclWI | GGATC | 1 cut(s) | 479 |
| AcyI | GRCGYC | 1 cut(s) | 411 |
| AfaI | GTAC | 3 cut(s) | 132, 138, 159 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 31, 85, 119, 217, 301 |
| AgeI | ACCGGT | 1 cut(s) | 119 |
| AjnI | CCWGG | 2 cut(s) | 296, 451 |
| AluBI | AGCT | 3 cut(s) | 10, 263, 307 |
| AluI | AGCT | 3 cut(s) | 10, 263, 307 |
| Alw21I | GWGCWC | 1 cut(s) | 48 |
| AlwI | GGATC | 1 cut(s) | 479 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 307, 335 |
| AoxI | GGCC | 5 cut(s) | 69, 75, 190, 267, 332 |
| ApaI | GGGCCC | 1 cut(s) | 194 |
| ApeKI | GCWGC | 2 cut(s) | 260, 373 |
| AsiGI | ACCGGT | 1 cut(s) | 119 |
| Asp718I | GGTACC | 2 cut(s) | 130, 157 |
| AspLEI | GCGC | 2 cut(s) | 413, 421 |
| AspS9I | GGNCC | 7 cut(s) | 22, 76, 190, 191, 267, 332, 455 |
| AsuHPI | GGTGA | 1 cut(s) | 454 |
| AvaII | GGWCC | 2 cut(s) | 22, 455 |
| BaeGI | GKGCMC | 1 cut(s) | 194 |
| BanI | GGYRCC | 3 cut(s) | 130, 157, 410 |
| BanII | GRGCYC | 1 cut(s) | 194 |
| Bbv12I | GWGCWC | 1 cut(s) | 48 |
| BbvI | GCAGC | 2 cut(s) | 272, 385 |
| BccI | CCATC | 1 cut(s) | 211 |
| BceAI | ACGGC | 1 cut(s) | 467 |
| BciT130I | CCWGG | 2 cut(s) | 298, 453 |
| BfaI | CTAG | 5 cut(s) | 173, 264, 362, 447, 468 |
| BfoI | RGCGCY | 2 cut(s) | 414, 422 |
| BfuAI | ACCTGC | 1 cut(s) | 145 |
| BisI | GCNGC | 2 cut(s) | 261, 374 |
| BlsI | GCNGC | 2 cut(s) | 262, 375 |
| Bme1390I | CCNGG | 2 cut(s) | 298, 453 |
| Bme18I | GGWCC | 2 cut(s) | 22, 455 |
| BmgT120I | GGNCC | 7 cut(s) | 22, 76, 190, 191, 267, 332, 455 |
| BmiI | GGNNCC | 4 cut(s) | 132, 159, 192, 412 |
| BmrFI | CCNGG | 2 cut(s) | 298, 453 |
| BmsI | GCATC | 1 cut(s) | 360 |
| BsaHI | GRCGYC | 1 cut(s) | 411 |
| BsaJI | CCNNGG | 2 cut(s) | 72, 296 |
| BsaWI | WCCGGW | 1 cut(s) | 119 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 31, 85, 119, 217, 301 |
| Bse118I | RCCGGY | 1 cut(s) | 119 |
| Bse1I | ACTGG | 1 cut(s) | 134 |
| BseBI | CCWGG | 2 cut(s) | 298, 453 |
| BseDI | CCNNGG | 2 cut(s) | 72, 296 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 31, 85, 119, 217, 301 |
| BseMII | CTCAG | 1 cut(s) | 267 |
| BseNI | ACTGG | 1 cut(s) | 134 |
| BseSI | GKGCMC | 1 cut(s) | 194 |
| BseXI | GCAGC | 2 cut(s) | 272, 385 |
| BseYI | CCCAGC | 1 cut(s) | 415 |
| BsgI | GTGCAG | 2 cut(s) | 188, 227 |
| BshFI | GGCC | 5 cut(s) | 71, 77, 192, 269, 334 |
| BshNI | GGYRCC | 3 cut(s) | 130, 157, 410 |
| BshTI | ACCGGT | 1 cut(s) | 119 |
| BsiHKAI | GWGCWC | 1 cut(s) | 48 |
| BsiSI | CCGG | 1 cut(s) | 120 |
| BslFI | GGGAC | 1 cut(s) | 278 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 31, 85, 119, 217, 301 |
| BsmFI | GGGAC | 1 cut(s) | 278 |
| BsnI | GGCC | 5 cut(s) | 71, 77, 192, 269, 334 |
| Bsp120I | GGGCCC | 1 cut(s) | 190 |
| Bsp1286I | GDGCHC | 2 cut(s) | 48, 194 |
| Bsp143I | GATC | 1 cut(s) | 471 |
| BspACI | CCGC | 3 cut(s) | 161, 208, 465 |
| BspANI | GGCC | 5 cut(s) | 71, 77, 192, 269, 334 |
| BspCNI | CTCAG | 1 cut(s) | 268 |
| BspLI | GGNNCC | 4 cut(s) | 132, 159, 192, 412 |
| BspMI | ACCTGC | 1 cut(s) | 145 |
| BspPI | GGATC | 1 cut(s) | 479 |
| BspT107I | GGYRCC | 3 cut(s) | 130, 157, 410 |
| BsrFI | RCCGGY | 1 cut(s) | 119 |
| BsrI | ACTGG | 1 cut(s) | 134 |
| BssAI | RCCGGY | 1 cut(s) | 119 |
| BssECI | CCNNGG | 2 cut(s) | 72, 296 |
| BssMI | GATC | 1 cut(s) | 471 |
| BssNI | GRCGYC | 1 cut(s) | 411 |
| Bst2UI | CCWGG | 2 cut(s) | 298, 453 |
| BstACI | GRCGYC | 1 cut(s) | 411 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 335 |
| BstC8I | GCNNGC | 4 cut(s) | 12, 336, 340, 409 |
| BstDEI | CTNAG | 1 cut(s) | 276 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 29 |
| BstH2I | RGCGCY | 2 cut(s) | 414, 422 |
| BstHHI | GCGC | 2 cut(s) | 413, 421 |
| BstKTI | GATC | 1 cut(s) | 474 |
| BstMBI | GATC | 1 cut(s) | 471 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 160, 266, 313, 335, 382 |
| BstNI | CCWGG | 2 cut(s) | 298, 453 |
| BstSCI | CCNGG | 2 cut(s) | 296, 451 |
| BstSLI | GKGCMC | 1 cut(s) | 194 |
| BstV1I | GCAGC | 2 cut(s) | 272, 385 |
| BstX2I | RGATCY | 1 cut(s) | 471 |
| BstYI | RGATCY | 1 cut(s) | 471 |
| BsuRI | GGCC | 5 cut(s) | 71, 77, 192, 269, 334 |
| BveI | ACCTGC | 1 cut(s) | 145 |
| Cac8I | GCNNGC | 4 cut(s) | 12, 336, 340, 409 |
| CaiI | CAGNNNCTG | 2 cut(s) | 307, 335 |
| CfoI | GCGC | 2 cut(s) | 413, 421 |
| Cfr10I | RCCGGY | 1 cut(s) | 119 |
| Cfr13I | GGNCC | 7 cut(s) | 22, 76, 190, 191, 267, 332, 455 |
| CseI | GACGC | 1 cut(s) | 432 |
| Csp6I | GTAC | 3 cut(s) | 131, 137, 158 |
| CspAI | ACCGGT | 1 cut(s) | 119 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 103, 138 |
| CviAII | CATG | 1 cut(s) | 224 |
| CviQI | GTAC | 3 cut(s) | 131, 137, 158 |
| DdeI | CTNAG | 1 cut(s) | 276 |
| DinI | GGCGCC | 1 cut(s) | 412 |
| DpnI | GATC | 1 cut(s) | 473 |
| DpnII | GATC | 1 cut(s) | 471 |
| Eco24I | GRGCYC | 1 cut(s) | 194 |
| Eco47I | GGWCC | 2 cut(s) | 22, 455 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 29 |
| EcoO109I | RGGNCCY | 1 cut(s) | 332 |
| EcoRII | CCWGG | 2 cut(s) | 296, 451 |
| EcoT38I | GRGCYC | 1 cut(s) | 194 |
| EgeI | GGCGCC | 1 cut(s) | 412 |
| EheI | GGCGCC | 1 cut(s) | 412 |
| FaeI | CATG | 1 cut(s) | 227 |
| FaiI | YATR | 6 cut(s) | 6, 146, 225, 347, 496, 498 |
| FaqI | GGGAC | 1 cut(s) | 278 |
| FatI | CATG | 1 cut(s) | 223 |
| FauNDI | CATATG | 1 cut(s) | 496 |
| Fnu4HI | GCNGC | 2 cut(s) | 261, 374 |
| FriOI | GRGCYC | 1 cut(s) | 194 |
| Fsp4HI | GCNGC | 2 cut(s) | 261, 374 |
| FspBI | CTAG | 5 cut(s) | 173, 264, 362, 447, 468 |
| GlaI | GCGC | 2 cut(s) | 412, 420 |
| GluI | GCNGC | 2 cut(s) | 261, 374 |
| GsaI | CCCAGC | 1 cut(s) | 419 |
| HaeII | RGCGCY | 2 cut(s) | 414, 422 |
| HaeIII | GGCC | 5 cut(s) | 71, 77, 192, 269, 334 |
| HapII | CCGG | 1 cut(s) | 120 |
| HgaI | GACGC | 1 cut(s) | 432 |
| HhaI | GCGC | 2 cut(s) | 413, 421 |
| Hin1I | GRCGYC | 1 cut(s) | 411 |
| Hin1II | CATG | 1 cut(s) | 227 |
| Hin6I | GCGC | 2 cut(s) | 411, 419 |
| HinP1I | GCGC | 2 cut(s) | 411, 419 |
| HinfI | GANTC | 2 cut(s) | 233, 319 |
| HpaII | CCGG | 1 cut(s) | 120 |
| HphI | GGTGA | 1 cut(s) | 454 |
| Hpy166II | GTNNAC | 2 cut(s) | 22, 91 |
| Hpy188III | TCNNGA | 1 cut(s) | 447 |
| Hpy8I | GTNNAC | 2 cut(s) | 22, 91 |
| HpyAV | CCTTC | 2 cut(s) | 61, 79 |
| HpyCH4V | TGCA | 5 cut(s) | 154, 205, 244, 373, 500 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 160, 266, 313, 335, 382 |
| HpyF3I | CTNAG | 1 cut(s) | 276 |
| Hsp92I | GRCGYC | 1 cut(s) | 411 |
| Hsp92II | CATG | 1 cut(s) | 227 |
| HspAI | GCGC | 2 cut(s) | 411, 419 |
| KasI | GGCGCC | 1 cut(s) | 410 |
| KpnI | GGTACC | 2 cut(s) | 134, 161 |
| Kzo9I | GATC | 1 cut(s) | 471 |
| LmnI | GCTCC | 2 cut(s) | 43, 304 |
| Lsp1109I | GCAGC | 2 cut(s) | 272, 385 |
| LweI | GCATC | 1 cut(s) | 360 |
| MaeI | CTAG | 5 cut(s) | 173, 264, 362, 447, 468 |
| MaeIII | GTNAC | 2 cut(s) | 234, 516 |
| MalI | GATC | 1 cut(s) | 473 |
| MboI | GATC | 1 cut(s) | 471 |
| MflI | RGATCY | 1 cut(s) | 471 |
| MhlI | GDGCHC | 2 cut(s) | 48, 194 |
| MluCI | AATT | 1 cut(s) | 377 |
| Mly113I | GGCGCC | 1 cut(s) | 411 |
| MlyI | GAGTC | 1 cut(s) | 242 |
| MnlI | CCTC | 5 cut(s) | 35, 67, 207, 271, 397 |
| MseI | TTAA | 1 cut(s) | 523 |
| MslI | CAYNNNNRTG | 1 cut(s) | 149 |
| MspI | CCGG | 1 cut(s) | 120 |
| MspR9I | CCNGG | 2 cut(s) | 298, 453 |
| MvaI | CCWGG | 2 cut(s) | 298, 453 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 160, 266, 313, 335, 382 |
| NarI | GGCGCC | 1 cut(s) | 411 |
| NdeI | CATATG | 1 cut(s) | 496 |
| NdeII | GATC | 1 cut(s) | 471 |
| NlaIII | CATG | 1 cut(s) | 227 |
| NlaIV | GGNNCC | 4 cut(s) | 132, 159, 192, 412 |
| NmeAIII | GCCGAG | 2 cut(s) | 97, 236 |
| NmuCI | GTSAC | 2 cut(s) | 234, 516 |
| PfeI | GAWTC | 1 cut(s) | 319 |
| PinAI | ACCGGT | 1 cut(s) | 119 |
| PkrI | GCNGC | 2 cut(s) | 262, 375 |
| PleI | GAGTC | 1 cut(s) | 241 |
| PluTI | GGCGCC | 1 cut(s) | 414 |
| PpsI | GAGTC | 1 cut(s) | 241 |
| Psp6I | CCWGG | 2 cut(s) | 296, 451 |
| PspFI | CCCAGC | 1 cut(s) | 415 |
| PspGI | CCWGG | 2 cut(s) | 296, 451 |
| PspN4I | GGNNCC | 4 cut(s) | 132, 159, 192, 412 |
| PspOMI | GGGCCC | 1 cut(s) | 190 |
| PspPI | GGNCC | 7 cut(s) | 22, 76, 190, 191, 267, 332, 455 |
| PstNI | CAGNNNCTG | 2 cut(s) | 307, 335 |
| PsuI | RGATCY | 1 cut(s) | 471 |
| RsaI | GTAC | 3 cut(s) | 132, 138, 159 |
| RsaNI | GTAC | 3 cut(s) | 131, 137, 158 |
| RseI | CAYNNNNRTG | 1 cut(s) | 149 |
| SaqAI | TTAA | 1 cut(s) | 523 |
| SatI | GCNGC | 2 cut(s) | 261, 374 |
| Sau3AI | GATC | 1 cut(s) | 471 |
| Sau96I | GGNCC | 7 cut(s) | 22, 76, 190, 191, 267, 332, 455 |
| SchI | GAGTC | 1 cut(s) | 242 |
| ScrFI | CCNGG | 2 cut(s) | 298, 453 |
| SduI | GDGCHC | 2 cut(s) | 48, 194 |
| SetI | ASST | 8 cut(s) | 12, 27, 90, 159, 199, 265, 292, 309 |
| SfaNI | GCATC | 1 cut(s) | 360 |
| SfoI | GGCGCC | 1 cut(s) | 412 |
| SinI | GGWCC | 2 cut(s) | 22, 455 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 149 |
| Sse9I | AATT | 1 cut(s) | 377 |
| SsiI | CCGC | 3 cut(s) | 161, 208, 465 |
| SspDI | GGCGCC | 1 cut(s) | 410 |
| SspMI | CTAG | 5 cut(s) | 173, 264, 362, 447, 468 |
| StyD4I | CCNGG | 2 cut(s) | 296, 451 |
| TaqI | TCGA | 1 cut(s) | 109 |
| TasI | AATT | 1 cut(s) | 377 |
| TfiI | GAWTC | 1 cut(s) | 319 |
| Tru1I | TTAA | 1 cut(s) | 523 |
| Tru9I | TTAA | 1 cut(s) | 523 |
| TseFI | GTSAC | 2 cut(s) | 234, 516 |
| TseI | GCWGC | 2 cut(s) | 260, 373 |
| Tsp45I | GTSAC | 2 cut(s) | 234, 516 |
| VpaK11BI | GGWCC | 2 cut(s) | 22, 455 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 29 |
| XbaI | TCTAGA | 1 cut(s) | 446 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 304 |
| XspI | CTAG | 5 cut(s) | 173, 264, 362, 447, 468 |