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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 192 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 259 |
| AciI | CCGC | 5 cut(s) | 20, 145, 185, 214, 299 |
| AclWI | GGATC | 2 cut(s) | 30, 286 |
| AcuI | CTGAAG | 1 cut(s) | 195 |
| AcyI | GRCGYC | 1 cut(s) | 309 |
| AfaI | GTAC | 1 cut(s) | 5 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 85, 259 |
| AflIII | ACRYGT | 1 cut(s) | 104 |
| AluBI | AGCT | 1 cut(s) | 10 |
| AluI | AGCT | 1 cut(s) | 10 |
| AlwI | GGATC | 2 cut(s) | 30, 286 |
| AoxI | GGCC | 2 cut(s) | 75, 135 |
| AspLEI | GCGC | 3 cut(s) | 60, 201, 319 |
| AspS9I | GGNCC | 1 cut(s) | 76 |
| AsuC2I | CCSGG | 2 cut(s) | 211, 296 |
| AsuNHI | GCTAGC | 1 cut(s) | 10 |
| BbvCI | CCTCAGC | 1 cut(s) | 72 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 230, 264 |
| BcnI | CCSGG | 2 cut(s) | 211, 296 |
| BfaI | CTAG | 2 cut(s) | 11, 131 |
| BfuAI | ACCTGC | 1 cut(s) | 192 |
| Bme1390I | CCNGG | 2 cut(s) | 211, 296 |
| BmgT120I | GGNCC | 1 cut(s) | 76 |
| BmrFI | CCNGG | 2 cut(s) | 211, 296 |
| BmsI | GCATC | 1 cut(s) | 232 |
| BmtI | GCTAGC | 1 cut(s) | 14 |
| BpmI | CTGGAG | 1 cut(s) | 299 |
| Bpu10I | CCTNAGC | 1 cut(s) | 72 |
| BpuMI | CCSGG | 2 cut(s) | 211, 296 |
| BsaHI | GRCGYC | 1 cut(s) | 309 |
| BsaJI | CCNNGG | 1 cut(s) | 194 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 272, 302 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 85, 259 |
| Bse1I | ACTGG | 2 cut(s) | 21, 282 |
| BseDI | CCNNGG | 1 cut(s) | 194 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 85, 259 |
| BseMII | CTCAG | 1 cut(s) | 63 |
| BseNI | ACTGG | 2 cut(s) | 21, 282 |
| BseYI | CCCAGC | 2 cut(s) | 148, 313 |
| BshFI | GGCC | 2 cut(s) | 77, 137 |
| BsiSI | CCGG | 2 cut(s) | 211, 295 |
| BslFI | GGGAC | 2 cut(s) | 193, 296 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 85, 259 |
| BsmFI | GGGAC | 2 cut(s) | 193, 296 |
| BsmI | GAATGC | 1 cut(s) | 273 |
| BsnI | GGCC | 2 cut(s) | 77, 137 |
| Bsp143I | GATC | 2 cut(s) | 22, 291 |
| BspACI | CCGC | 5 cut(s) | 20, 145, 185, 214, 299 |
| BspANI | GGCC | 2 cut(s) | 77, 137 |
| BspCNI | CTCAG | 1 cut(s) | 64 |
| BspMI | ACCTGC | 1 cut(s) | 192 |
| BspOI | GCTAGC | 1 cut(s) | 14 |
| BspPI | GGATC | 2 cut(s) | 30, 286 |
| BsrI | ACTGG | 2 cut(s) | 21, 282 |
| BssECI | CCNNGG | 1 cut(s) | 194 |
| BssMI | GATC | 2 cut(s) | 22, 291 |
| BssNI | GRCGYC | 1 cut(s) | 309 |
| BssT1I | CCWWGG | 1 cut(s) | 194 |
| BstACI | GRCGYC | 1 cut(s) | 309 |
| BstC8I | GCNNGC | 3 cut(s) | 12, 161, 165 |
| BstDEI | CTNAG | 1 cut(s) | 72 |
| BstHHI | GCGC | 3 cut(s) | 60, 201, 319 |
| BstKTI | GATC | 2 cut(s) | 25, 294 |
| BstMBI | GATC | 2 cut(s) | 22, 291 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 233, 242 |
| BstSCI | CCNGG | 2 cut(s) | 209, 294 |
| BstX2I | RGATCY | 1 cut(s) | 22 |
| BstYI | RGATCY | 1 cut(s) | 22 |
| BsuRI | GGCC | 2 cut(s) | 77, 137 |
| BtsIMutI | CAGTG | 2 cut(s) | 14, 138 |
| BveI | ACCTGC | 1 cut(s) | 192 |
| Cac8I | GCNNGC | 3 cut(s) | 12, 161, 165 |
| CfoI | GCGC | 3 cut(s) | 60, 201, 319 |
| Cfr13I | GGNCC | 1 cut(s) | 76 |
| CseI | GACGC | 1 cut(s) | 298 |
| Csp6I | GTAC | 1 cut(s) | 4 |
| CviJI | RGCY | 6 cut(s) | 10, 71, 77, 92, 137, 227 |
| CviKI_1 | RGCY | 6 cut(s) | 10, 71, 77, 92, 137, 227 |
| CviQI | GTAC | 1 cut(s) | 4 |
| DdeI | CTNAG | 1 cut(s) | 72 |
| DpnI | GATC | 2 cut(s) | 24, 293 |
| DpnII | GATC | 2 cut(s) | 22, 291 |
| EciI | GGCGGA | 3 cut(s) | 35, 174, 314 |
| Eco130I | CCWWGG | 1 cut(s) | 194 |
| Eco57I | CTGAAG | 1 cut(s) | 195 |
| EcoT14I | CCWWGG | 1 cut(s) | 194 |
| ErhI | CCWWGG | 1 cut(s) | 194 |
| FaiI | YATR | 2 cut(s) | 30, 101 |
| FaqI | GGGAC | 2 cut(s) | 193, 296 |
| FauI | CCCGC | 1 cut(s) | 207 |
| FspBI | CTAG | 2 cut(s) | 11, 131 |
| GlaI | GCGC | 3 cut(s) | 59, 200, 318 |
| GsaI | CCCAGC | 2 cut(s) | 152, 317 |
| GsuI | CTGGAG | 1 cut(s) | 299 |
| HaeIII | GGCC | 2 cut(s) | 77, 137 |
| HapII | CCGG | 2 cut(s) | 211, 295 |
| HgaI | GACGC | 1 cut(s) | 298 |
| HhaI | GCGC | 3 cut(s) | 60, 201, 319 |
| Hin1I | GRCGYC | 1 cut(s) | 309 |
| Hin6I | GCGC | 3 cut(s) | 58, 199, 317 |
| HinP1I | GCGC | 3 cut(s) | 58, 199, 317 |
| HinfI | GANTC | 2 cut(s) | 230, 302 |
| HpaII | CCGG | 2 cut(s) | 211, 295 |
| Hpy99I | CGWCG | 1 cut(s) | 242 |
| HpyAV | CCTTC | 2 cut(s) | 46, 170 |
| HpyCH4IV | ACGT | 2 cut(s) | 46, 106 |
| HpyCH4V | TGCA | 2 cut(s) | 245, 271 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 233, 242 |
| HpyF3I | CTNAG | 1 cut(s) | 72 |
| HpySE526I | ACGT | 2 cut(s) | 46, 106 |
| Hsp92I | GRCGYC | 1 cut(s) | 309 |
| HspAI | GCGC | 3 cut(s) | 58, 199, 317 |
| Kzo9I | GATC | 2 cut(s) | 22, 291 |
| LmnI | GCTCC | 2 cut(s) | 55, 280 |
| LweI | GCATC | 1 cut(s) | 232 |
| MaeI | CTAG | 2 cut(s) | 11, 131 |
| MaeII | ACGT | 2 cut(s) | 46, 106 |
| MalI | GATC | 2 cut(s) | 24, 293 |
| MboI | GATC | 2 cut(s) | 22, 291 |
| MboII | GAAGA | 1 cut(s) | 17 |
| MflI | RGATCY | 1 cut(s) | 22 |
| MlyI | GAGTC | 1 cut(s) | 311 |
| MmeI | TCCRAC | 1 cut(s) | 240 |
| MnlI | CCTC | 5 cut(s) | 67, 106, 127, 162, 279 |
| MspI | CCGG | 2 cut(s) | 211, 295 |
| MspR9I | CCNGG | 2 cut(s) | 211, 296 |
| Mva1269I | GAATGC | 1 cut(s) | 273 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 233, 242 |
| NciI | CCSGG | 2 cut(s) | 211, 296 |
| NdeII | GATC | 2 cut(s) | 22, 291 |
| NheI | GCTAGC | 1 cut(s) | 10 |
| PctI | GAATGC | 1 cut(s) | 273 |
| PfeI | GAWTC | 1 cut(s) | 230 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 259 |
| PleI | GAGTC | 1 cut(s) | 310 |
| PpsI | GAGTC | 1 cut(s) | 310 |
| PspFI | CCCAGC | 2 cut(s) | 148, 313 |
| PspPI | GGNCC | 1 cut(s) | 76 |
| PsuI | RGATCY | 1 cut(s) | 22 |
| RsaI | GTAC | 1 cut(s) | 5 |
| RsaNI | GTAC | 1 cut(s) | 4 |
| Sau3AI | GATC | 2 cut(s) | 22, 291 |
| Sau96I | GGNCC | 1 cut(s) | 76 |
| SchI | GAGTC | 1 cut(s) | 311 |
| ScrFI | CCNGG | 2 cut(s) | 211, 296 |
| SetI | ASST | 6 cut(s) | 12, 49, 90, 109, 206, 290 |
| SfaNI | GCATC | 1 cut(s) | 232 |
| SsiI | CCGC | 5 cut(s) | 20, 145, 185, 214, 299 |
| SspMI | CTAG | 2 cut(s) | 11, 131 |
| StyD4I | CCNGG | 2 cut(s) | 209, 294 |
| StyI | CCWWGG | 1 cut(s) | 194 |
| TaiI | ACGT | 2 cut(s) | 49, 109 |
| TfiI | GAWTC | 1 cut(s) | 230 |
| TscAI | CASTG | 2 cut(s) | 21, 145 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspRI | CASTG | 2 cut(s) | 21, 145 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 259 |
| XspI | CTAG | 2 cut(s) | 11, 131 |