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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 174 |
| Acc36I | ACCTGC | 1 cut(s) | 174 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 378 |
| AccII | CGCG | 3 cut(s) | 146, 267, 404 |
| AciI | CCGC | 4 cut(s) | 49, 267, 404, 414 |
| AcoI | YGGCCR | 1 cut(s) | 188 |
| AcsI | RAATTY | 1 cut(s) | 205 |
| AcuI | CTGAAG | 1 cut(s) | 348 |
| AfaI | GTAC | 1 cut(s) | 520 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 48, 183, 310, 378 |
| AgsI | TTSAA | 1 cut(s) | 27 |
| AjiI | CACGTC | 1 cut(s) | 420 |
| AjnI | CCWGG | 1 cut(s) | 41 |
| AleI | CACNNNNGTG | 1 cut(s) | 422 |
| AluBI | AGCT | 3 cut(s) | 330, 375, 384 |
| AluI | AGCT | 3 cut(s) | 330, 375, 384 |
| Alw21I | GWGCWC | 3 cut(s) | 21, 274, 332 |
| Alw26I | GTCTC | 1 cut(s) | 46 |
| Alw44I | GTGCAC | 1 cut(s) | 17 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 378 |
| AoxI | GGCC | 3 cut(s) | 72, 167, 188 |
| ApaLI | GTGCAC | 1 cut(s) | 17 |
| ApeKI | GCWGC | 2 cut(s) | 65, 94 |
| ApoI | RAATTY | 1 cut(s) | 205 |
| AspLEI | GCGC | 4 cut(s) | 146, 158, 228, 404 |
| AspS9I | GGNCC | 3 cut(s) | 73, 87, 120 |
| AsuC2I | CCSGG | 3 cut(s) | 62, 85, 445 |
| AsuHPI | GGTGA | 2 cut(s) | 439, 450 |
| AsuNHI | GCTAGC | 1 cut(s) | 241 |
| AvaII | GGWCC | 2 cut(s) | 87, 120 |
| BaeGI | GKGCMC | 1 cut(s) | 21 |
| BalI | TGGCCA | 1 cut(s) | 190 |
| BanII | GRGCYC | 1 cut(s) | 332 |
| Bbv12I | GWGCWC | 3 cut(s) | 21, 274, 332 |
| BbvI | GCAGC | 2 cut(s) | 77, 81 |
| BciT130I | CCWGG | 1 cut(s) | 43 |
| BcnI | CCSGG | 3 cut(s) | 62, 85, 445 |
| BcoDI | GTCTC | 1 cut(s) | 46 |
| BfaI | CTAG | 1 cut(s) | 242 |
| BfoI | RGCGCY | 1 cut(s) | 229 |
| BfuAI | ACCTGC | 1 cut(s) | 174 |
| BisI | GCNGC | 3 cut(s) | 66, 95, 415 |
| BlsI | GCNGC | 3 cut(s) | 67, 96, 416 |
| Bme1390I | CCNGG | 4 cut(s) | 43, 62, 85, 445 |
| Bme18I | GGWCC | 2 cut(s) | 87, 120 |
| BmgBI | CACGTC | 1 cut(s) | 420 |
| BmgT120I | GGNCC | 3 cut(s) | 73, 87, 120 |
| BmiI | GGNNCC | 1 cut(s) | 88 |
| BmrFI | CCNGG | 4 cut(s) | 43, 62, 85, 445 |
| BmsI | GCATC | 1 cut(s) | 380 |
| BmtI | GCTAGC | 1 cut(s) | 245 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 517 |
| BpmI | CTGGAG | 2 cut(s) | 64, 400 |
| Bpu10I | CCTNAGC | 1 cut(s) | 450 |
| BpuEI | CTTGAG | 1 cut(s) | 250 |
| BpuMI | CCSGG | 3 cut(s) | 62, 85, 445 |
| BsaI | GGTCTC | 1 cut(s) | 46 |
| BsaJI | CCNNGG | 1 cut(s) | 444 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 48, 183, 310, 378 |
| Bse1I | ACTGG | 1 cut(s) | 113 |
| BseBI | CCWGG | 1 cut(s) | 43 |
| BseDI | CCNNGG | 1 cut(s) | 444 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 48, 183, 310, 378 |
| BseMII | CTCAG | 1 cut(s) | 294 |
| BseNI | ACTGG | 1 cut(s) | 113 |
| BseRI | GAGGAG | 1 cut(s) | 309 |
| BseSI | GKGCMC | 1 cut(s) | 21 |
| BseXI | GCAGC | 2 cut(s) | 77, 81 |
| BseYI | CCCAGC | 2 cut(s) | 56, 371 |
| BsgI | GTGCAG | 1 cut(s) | 233 |
| Bsh1236I | CGCG | 3 cut(s) | 146, 267, 404 |
| BshFI | GGCC | 3 cut(s) | 74, 169, 190 |
| BsiHKAI | GWGCWC | 3 cut(s) | 21, 274, 332 |
| BsiSI | CCGG | 3 cut(s) | 61, 84, 444 |
| BslFI | GGGAC | 3 cut(s) | 100, 353, 365 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 48, 183, 310, 378 |
| BsmAI | GTCTC | 1 cut(s) | 46 |
| BsmFI | GGGAC | 3 cut(s) | 100, 353, 365 |
| BsnI | GGCC | 3 cut(s) | 74, 169, 190 |
| Bso31I | GGTCTC | 1 cut(s) | 46 |
| Bsp1286I | GDGCHC | 3 cut(s) | 21, 274, 332 |
| Bsp143I | GATC | 1 cut(s) | 429 |
| BspACI | CCGC | 4 cut(s) | 49, 267, 404, 414 |
| BspANI | GGCC | 3 cut(s) | 74, 169, 190 |
| BspCNI | CTCAG | 1 cut(s) | 293 |
| BspFNI | CGCG | 3 cut(s) | 146, 267, 404 |
| BspLI | GGNNCC | 1 cut(s) | 88 |
| BspMI | ACCTGC | 1 cut(s) | 174 |
| BspOI | GCTAGC | 1 cut(s) | 245 |
| BspQI | GCTCTTC | 1 cut(s) | 337 |
| BspTNI | GGTCTC | 1 cut(s) | 46 |
| BsrI | ACTGG | 1 cut(s) | 113 |
| BssECI | CCNNGG | 1 cut(s) | 444 |
| BssMI | GATC | 1 cut(s) | 429 |
| Bst2UI | CCWGG | 1 cut(s) | 43 |
| Bst4CI | ACNGT | 2 cut(s) | 16, 492 |
| Bst6I | CTCTTC | 2 cut(s) | 314, 337 |
| BstC8I | GCNNGC | 3 cut(s) | 243, 259, 514 |
| BstDEI | CTNAG | 2 cut(s) | 280, 450 |
| BstEII | GGTNACC | 1 cut(s) | 457 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 308 |
| BstFNI | CGCG | 3 cut(s) | 146, 267, 404 |
| BstH2I | RGCGCY | 1 cut(s) | 229 |
| BstHHI | GCGC | 4 cut(s) | 146, 158, 228, 404 |
| BstKTI | GATC | 1 cut(s) | 432 |
| BstMAI | GTCTC | 1 cut(s) | 46 |
| BstMBI | GATC | 1 cut(s) | 429 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 71, 232, 247, 381, 390, 399 |
| BstNI | CCWGG | 1 cut(s) | 43 |
| BstPI | GGTNACC | 1 cut(s) | 457 |
| BstSCI | CCNGG | 4 cut(s) | 41, 60, 83, 443 |
| BstSLI | GKGCMC | 1 cut(s) | 21 |
| BstUI | CGCG | 3 cut(s) | 146, 267, 404 |
| BstV1I | GCAGC | 2 cut(s) | 77, 81 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 529 |
| BsuRI | GGCC | 3 cut(s) | 74, 169, 190 |
| BtrI | CACGTC | 1 cut(s) | 420 |
| BtsI | GCAGTG | 2 cut(s) | 75, 245 |
| BtsIMutI | CAGTG | 3 cut(s) | 75, 245, 429 |
| BveI | ACCTGC | 1 cut(s) | 174 |
| Cac8I | GCNNGC | 3 cut(s) | 243, 259, 514 |
| CaiI | CAGNNNCTG | 1 cut(s) | 378 |
| CfoI | GCGC | 4 cut(s) | 146, 158, 228, 404 |
| Cfr13I | GGNCC | 3 cut(s) | 73, 87, 120 |
| Csp6I | GTAC | 1 cut(s) | 519 |
| CviAII | CATG | 3 cut(s) | 286, 337, 523 |
| CviQI | GTAC | 1 cut(s) | 519 |
| DdeI | CTNAG | 2 cut(s) | 280, 450 |
| DpnI | GATC | 1 cut(s) | 431 |
| DpnII | GATC | 1 cut(s) | 429 |
| EaeI | YGGCCR | 1 cut(s) | 188 |
| Eam1104I | CTCTTC | 2 cut(s) | 314, 337 |
| EarI | CTCTTC | 2 cut(s) | 314, 337 |
| EciI | GGCGGA | 1 cut(s) | 64 |
| Ecl136II | GAGCTC | 1 cut(s) | 330 |
| Eco24I | GRGCYC | 1 cut(s) | 332 |
| Eco31I | GGTCTC | 1 cut(s) | 46 |
| Eco47I | GGWCC | 2 cut(s) | 87, 120 |
| Eco53kI | GAGCTC | 1 cut(s) | 330 |
| Eco57I | CTGAAG | 1 cut(s) | 348 |
| Eco91I | GGTNACC | 1 cut(s) | 457 |
| EcoICRI | GAGCTC | 1 cut(s) | 330 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 308 |
| EcoO109I | RGGNCCY | 1 cut(s) | 87 |
| EcoO65I | GGTNACC | 1 cut(s) | 457 |
| EcoRII | CCWGG | 1 cut(s) | 41 |
| EcoT38I | GRGCYC | 1 cut(s) | 332 |
| FaeI | CATG | 3 cut(s) | 289, 340, 526 |
| FaiI | YATR | 8 cut(s) | 100, 104, 160, 287, 310, 338, 524, 540 |
| FaqI | GGGAC | 3 cut(s) | 100, 353, 365 |
| FatI | CATG | 3 cut(s) | 285, 336, 522 |
| Fnu4HI | GCNGC | 3 cut(s) | 66, 95, 415 |
| FriOI | GRGCYC | 1 cut(s) | 332 |
| Fsp4HI | GCNGC | 3 cut(s) | 66, 95, 415 |
| FspBI | CTAG | 1 cut(s) | 242 |
| GlaI | GCGC | 4 cut(s) | 145, 157, 227, 403 |
| GluI | GCNGC | 3 cut(s) | 66, 95, 415 |
| GsaI | CCCAGC | 2 cut(s) | 60, 375 |
| GsuI | CTGGAG | 2 cut(s) | 64, 400 |
| HaeII | RGCGCY | 1 cut(s) | 229 |
| HaeIII | GGCC | 3 cut(s) | 74, 169, 190 |
| HapII | CCGG | 3 cut(s) | 61, 84, 444 |
| HhaI | GCGC | 4 cut(s) | 146, 158, 228, 404 |
| Hin1II | CATG | 3 cut(s) | 289, 340, 526 |
| Hin6I | GCGC | 4 cut(s) | 144, 156, 226, 402 |
| HinP1I | GCGC | 4 cut(s) | 144, 156, 226, 402 |
| HincII | GTYRAC | 2 cut(s) | 12, 495 |
| HindII | GTYRAC | 2 cut(s) | 12, 495 |
| HpaI | GTTAAC | 1 cut(s) | 12 |
| HpaII | CCGG | 3 cut(s) | 61, 84, 444 |
| HphI | GGTGA | 2 cut(s) | 439, 450 |
| Hpy166II | GTNNAC | 4 cut(s) | 12, 19, 441, 495 |
| Hpy188III | TCNNGA | 1 cut(s) | 379 |
| Hpy8I | GTNNAC | 4 cut(s) | 12, 19, 441, 495 |
| Hpy99I | CGWCG | 1 cut(s) | 214 |
| HpyAV | CCTTC | 1 cut(s) | 159 |
| HpyCH4III | ACNGT | 2 cut(s) | 16, 492 |
| HpyCH4IV | ACGT | 1 cut(s) | 419 |
| HpyCH4V | TGCA | 2 cut(s) | 19, 250 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 71, 232, 247, 381, 390, 399 |
| HpyF3I | CTNAG | 2 cut(s) | 280, 450 |
| HpySE526I | ACGT | 1 cut(s) | 419 |
| Hsp92II | CATG | 3 cut(s) | 289, 340, 526 |
| HspAI | GCGC | 4 cut(s) | 144, 156, 226, 402 |
| KspAI | GTTAAC | 1 cut(s) | 12 |
| Kzo9I | GATC | 1 cut(s) | 429 |
| LguI | GCTCTTC | 1 cut(s) | 337 |
| LmnI | GCTCC | 4 cut(s) | 141, 153, 269, 381 |
| Lsp1109I | GCAGC | 2 cut(s) | 77, 81 |
| LweI | GCATC | 1 cut(s) | 380 |
| MaeI | CTAG | 1 cut(s) | 242 |
| MaeII | ACGT | 1 cut(s) | 419 |
| MaeIII | GTNAC | 3 cut(s) | 457, 484, 505 |
| MalI | GATC | 1 cut(s) | 431 |
| MboI | GATC | 1 cut(s) | 429 |
| MboII | GAAGA | 2 cut(s) | 324, 331 |
| MhlI | GDGCHC | 3 cut(s) | 21, 274, 332 |
| MlsI | TGGCCA | 1 cut(s) | 190 |
| MluCI | AATT | 2 cut(s) | 205, 214 |
| MluNI | TGGCCA | 1 cut(s) | 190 |
| MnlI | CCTC | 6 cut(s) | 39, 100, 133, 225, 287, 315 |
| Mox20I | TGGCCA | 1 cut(s) | 190 |
| MscI | TGGCCA | 1 cut(s) | 190 |
| MseI | TTAA | 2 cut(s) | 11, 217 |
| MslI | CAYNNNNRTG | 3 cut(s) | 196, 422, 527 |
| Msp20I | TGGCCA | 1 cut(s) | 190 |
| MspI | CCGG | 3 cut(s) | 61, 84, 444 |
| MspR9I | CCNGG | 4 cut(s) | 43, 62, 85, 445 |
| MvaI | CCWGG | 1 cut(s) | 43 |
| MvnI | CGCG | 3 cut(s) | 146, 267, 404 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 71, 232, 247, 381, 390, 399 |
| NciI | CCSGG | 3 cut(s) | 62, 85, 445 |
| NdeII | GATC | 1 cut(s) | 429 |
| NheI | GCTAGC | 1 cut(s) | 241 |
| NlaIII | CATG | 3 cut(s) | 289, 340, 526 |
| NlaIV | GGNNCC | 1 cut(s) | 88 |
| NmuCI | GTSAC | 1 cut(s) | 484 |
| OliI | CACNNNNGTG | 1 cut(s) | 422 |
| PaqCI | CACCTGC | 1 cut(s) | 174 |
| PciSI | GCTCTTC | 1 cut(s) | 337 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 378 |
| PfoI | TCCNGGA | 1 cut(s) | 83 |
| PkrI | GCNGC | 3 cut(s) | 67, 96, 416 |
| PpuMI | RGGWCCY | 1 cut(s) | 87 |
| Psp124BI | GAGCTC | 1 cut(s) | 332 |
| Psp5II | RGGWCCY | 1 cut(s) | 87 |
| Psp6I | CCWGG | 1 cut(s) | 41 |
| PspEI | GGTNACC | 1 cut(s) | 457 |
| PspFI | CCCAGC | 2 cut(s) | 56, 371 |
| PspGI | CCWGG | 1 cut(s) | 41 |
| PspN4I | GGNNCC | 1 cut(s) | 88 |
| PspPI | GGNCC | 3 cut(s) | 73, 87, 120 |
| PspPPI | RGGWCCY | 1 cut(s) | 87 |
| PstNI | CAGNNNCTG | 1 cut(s) | 378 |
| RsaI | GTAC | 1 cut(s) | 520 |
| RsaNI | GTAC | 1 cut(s) | 519 |
| RseI | CAYNNNNRTG | 3 cut(s) | 196, 422, 527 |
| SacI | GAGCTC | 1 cut(s) | 332 |
| SapI | GCTCTTC | 1 cut(s) | 337 |
| SaqAI | TTAA | 2 cut(s) | 11, 217 |
| SatI | GCNGC | 3 cut(s) | 66, 95, 415 |
| Sau3AI | GATC | 1 cut(s) | 429 |
| Sau96I | GGNCC | 3 cut(s) | 73, 87, 120 |
| ScrFI | CCNGG | 4 cut(s) | 43, 62, 85, 445 |
| SduI | GDGCHC | 3 cut(s) | 21, 274, 332 |
| SfaNI | GCATC | 1 cut(s) | 380 |
| SinI | GGWCC | 2 cut(s) | 87, 120 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 196, 422, 527 |
| SmlI | CTYRAG | 1 cut(s) | 229 |
| SmoI | CTYRAG | 1 cut(s) | 229 |
| Sse9I | AATT | 2 cut(s) | 205, 214 |
| SsiI | CCGC | 4 cut(s) | 49, 267, 404, 414 |
| SspMI | CTAG | 1 cut(s) | 242 |
| SstI | GAGCTC | 1 cut(s) | 332 |
| StyD4I | CCNGG | 4 cut(s) | 41, 60, 83, 443 |
| TaaI | ACNGT | 2 cut(s) | 16, 492 |
| TaiI | ACGT | 1 cut(s) | 422 |
| TaqI | TCGA | 1 cut(s) | 209 |
| TasI | AATT | 2 cut(s) | 205, 214 |
| TauI | GCSGC | 1 cut(s) | 417 |
| Tru1I | TTAA | 2 cut(s) | 11, 217 |
| Tru9I | TTAA | 2 cut(s) | 11, 217 |
| TscAI | CASTG | 3 cut(s) | 75, 252, 429 |
| TseFI | GTSAC | 1 cut(s) | 484 |
| TseI | GCWGC | 2 cut(s) | 65, 94 |
| Tsp45I | GTSAC | 1 cut(s) | 484 |
| TspDTI | ATGAA | 1 cut(s) | 119 |
| TspGWI | ACGGA | 2 cut(s) | 133, 468 |
| TspRI | CASTG | 3 cut(s) | 75, 252, 429 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 378 |
| VneI | GTGCAC | 1 cut(s) | 17 |
| VpaK11BI | GGWCC | 2 cut(s) | 87, 120 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 308 |
| XapI | RAATTY | 1 cut(s) | 205 |
| XspI | CTAG | 1 cut(s) | 242 |