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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 199, 329 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 740 |
| Acc36I | ACCTGC | 3 cut(s) | 199, 329, 346 |
| Acc65I | GGTACC | 1 cut(s) | 358 |
| AccB1I | GGYRCC | 2 cut(s) | 358, 605 |
| AccBSI | CCGCTC | 1 cut(s) | 562 |
| AclI | AACGTT | 1 cut(s) | 84 |
| AclWI | GGATC | 2 cut(s) | 394, 661 |
| AcoI | YGGCCR | 1 cut(s) | 213 |
| AcuI | CTGAAG | 1 cut(s) | 275 |
| AcyI | GRCGYC | 1 cut(s) | 606 |
| AfaI | GTAC | 1 cut(s) | 360 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 208, 556 |
| AgsI | TTSAA | 1 cut(s) | 760 |
| AjnI | CCWGG | 3 cut(s) | 491, 519, 646 |
| AluBI | AGCT | 6 cut(s) | 133, 241, 379, 450, 502, 586 |
| AluI | AGCT | 6 cut(s) | 133, 241, 379, 450, 502, 586 |
| Alw21I | GWGCWC | 2 cut(s) | 97, 299 |
| Alw26I | GTCTC | 1 cut(s) | 531 |
| Alw44I | GTGCAC | 1 cut(s) | 93 |
| AlwI | GGATC | 2 cut(s) | 394, 661 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 659 |
| Ama87I | CYCGRG | 1 cut(s) | 530 |
| AoxI | GGCC | 4 cut(s) | 45, 192, 198, 213 |
| ApaLI | GTGCAC | 1 cut(s) | 93 |
| ApeKI | GCWGC | 1 cut(s) | 447 |
| AseI | ATTAAT | 1 cut(s) | 329 |
| Asp718I | GGTACC | 1 cut(s) | 358 |
| AspLEI | GCGC | 4 cut(s) | 277, 608, 616, 691 |
| AspS9I | GGNCC | 6 cut(s) | 112, 145, 199, 338, 527, 650 |
| AsuC2I | CCSGG | 4 cut(s) | 336, 531, 532, 686 |
| AsuHPI | GGTGA | 3 cut(s) | 109, 381, 760 |
| AvaI | CYCGRG | 1 cut(s) | 530 |
| AvaII | GGWCC | 5 cut(s) | 112, 145, 338, 527, 650 |
| BaeGI | GKGCMC | 1 cut(s) | 97 |
| BalI | TGGCCA | 1 cut(s) | 215 |
| BanI | GGYRCC | 2 cut(s) | 358, 605 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 465, 497 |
| Bbv12I | GWGCWC | 2 cut(s) | 97, 299 |
| BbvCI | CCTCAGC | 2 cut(s) | 27, 597 |
| BbvI | GCAGC | 1 cut(s) | 459 |
| BccI | CCATC | 1 cut(s) | 686 |
| BciT130I | CCWGG | 3 cut(s) | 493, 521, 648 |
| BclI | TGATCA | 1 cut(s) | 60 |
| BcnI | CCSGG | 4 cut(s) | 336, 531, 532, 686 |
| BcoDI | GTCTC | 1 cut(s) | 531 |
| BfaI | CTAG | 2 cut(s) | 140, 575 |
| BfoI | RGCGCY | 2 cut(s) | 609, 617 |
| BfuAI | ACCTGC | 3 cut(s) | 199, 329, 346 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 44 |
| BisI | GCNGC | 2 cut(s) | 448, 724 |
| BlsI | GCNGC | 2 cut(s) | 449, 725 |
| Bme1390I | CCNGG | 7 cut(s) | 336, 493, 521, 531, 532, 648, 686 |
| Bme18I | GGWCC | 5 cut(s) | 112, 145, 338, 527, 650 |
| BmeT110I | CYCGRG | 1 cut(s) | 530 |
| BmgT120I | GGNCC | 6 cut(s) | 112, 145, 199, 338, 527, 650 |
| BmiI | GGNNCC | 4 cut(s) | 360, 528, 529, 607 |
| BmrFI | CCNGG | 7 cut(s) | 336, 493, 521, 531, 532, 648, 686 |
| BmrI | ACTGGG | 1 cut(s) | 392 |
| BmsI | GCATC | 1 cut(s) | 555 |
| BmuI | ACTGGG | 1 cut(s) | 392 |
| BpmI | CTGGAG | 1 cut(s) | 578 |
| Bpu10I | CCTNAGC | 2 cut(s) | 27, 597 |
| BpuMI | CCSGG | 4 cut(s) | 336, 531, 532, 686 |
| BsaAI | YACGTR | 1 cut(s) | 694 |
| BsaBI | GATNNNNATC | 1 cut(s) | 65 |
| BsaHI | GRCGYC | 1 cut(s) | 606 |
| BsaJI | CCNNGG | 4 cut(s) | 195, 491, 530, 531 |
| BsaWI | WCCGGW | 2 cut(s) | 109, 373 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 413, 443 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 208, 556 |
| Bse1I | ACTGG | 3 cut(s) | 387, 561, 653 |
| Bse8I | GATNNNNATC | 1 cut(s) | 65 |
| BseBI | CCWGG | 3 cut(s) | 493, 521, 648 |
| BseDI | CCNNGG | 4 cut(s) | 195, 491, 530, 531 |
| BseGI | GGATG | 3 cut(s) | 516, 570, 678 |
| BseJI | GATNNNNATC | 1 cut(s) | 65 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 208, 556 |
| BseMII | CTCAG | 2 cut(s) | 18, 588 |
| BseNI | ACTGG | 3 cut(s) | 387, 561, 653 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseSI | GKGCMC | 1 cut(s) | 97 |
| BseXI | GCAGC | 1 cut(s) | 459 |
| BseYI | CCCAGC | 2 cut(s) | 271, 610 |
| BsgI | GTGCAG | 1 cut(s) | 374 |
| BshFI | GGCC | 4 cut(s) | 47, 194, 200, 215 |
| BshNI | GGYRCC | 2 cut(s) | 358, 605 |
| BsiHKAI | GWGCWC | 2 cut(s) | 97, 299 |
| BsiHKCI | CYCGRG | 1 cut(s) | 530 |
| BsiSI | CCGG | 5 cut(s) | 110, 336, 374, 531, 686 |
| BslFI | GGGAC | 1 cut(s) | 540 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 208, 556 |
| BsmAI | GTCTC | 1 cut(s) | 531 |
| BsmFI | GGGAC | 1 cut(s) | 540 |
| BsnI | GGCC | 4 cut(s) | 47, 194, 200, 215 |
| BsoBI | CYCGRG | 1 cut(s) | 530 |
| Bsp1286I | GDGCHC | 2 cut(s) | 97, 299 |
| Bsp143I | GATC | 4 cut(s) | 40, 60, 386, 666 |
| BspANI | GGCC | 4 cut(s) | 47, 194, 200, 215 |
| BspCNI | CTCAG | 2 cut(s) | 19, 589 |
| BspLI | GGNNCC | 4 cut(s) | 360, 528, 529, 607 |
| BspMI | ACCTGC | 3 cut(s) | 199, 329, 346 |
| BspPI | GGATC | 2 cut(s) | 394, 661 |
| BspT107I | GGYRCC | 2 cut(s) | 358, 605 |
| BsrBI | CCGCTC | 1 cut(s) | 562 |
| BsrI | ACTGG | 3 cut(s) | 387, 561, 653 |
| BssECI | CCNNGG | 4 cut(s) | 195, 491, 530, 531 |
| BssMI | GATC | 4 cut(s) | 40, 60, 386, 666 |
| BssNI | GRCGYC | 1 cut(s) | 606 |
| Bst2UI | CCWGG | 3 cut(s) | 493, 521, 648 |
| Bst4CI | ACNGT | 1 cut(s) | 92 |
| BstACI | GRCGYC | 1 cut(s) | 606 |
| BstBAI | YACGTR | 1 cut(s) | 694 |
| BstC8I | GCNNGC | 5 cut(s) | 36, 135, 284, 604, 675 |
| BstDEI | CTNAG | 2 cut(s) | 27, 597 |
| BstF5I | GGATG | 3 cut(s) | 516, 570, 678 |
| BstH2I | RGCGCY | 2 cut(s) | 609, 617 |
| BstHHI | GCGC | 4 cut(s) | 277, 608, 616, 691 |
| BstKTI | GATC | 4 cut(s) | 43, 63, 389, 669 |
| BstMAI | GTCTC | 1 cut(s) | 531 |
| BstMBI | GATC | 4 cut(s) | 40, 60, 386, 666 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 44, 272, 283, 303, 361, 508 |
| BstNI | CCWGG | 3 cut(s) | 493, 521, 648 |
| BstNSI | RCATGY | 1 cut(s) | 677 |
| BstSCI | CCNGG | 7 cut(s) | 334, 491, 519, 529, 530, 646, 684 |
| BstSLI | GKGCMC | 1 cut(s) | 97 |
| BstV1I | GCAGC | 1 cut(s) | 459 |
| BstX2I | RGATCY | 1 cut(s) | 666 |
| BstYI | RGATCY | 1 cut(s) | 666 |
| BsuRI | GGCC | 4 cut(s) | 47, 194, 200, 215 |
| BtsCI | GGATG | 3 cut(s) | 516, 570, 678 |
| BtsI | GCAGTG | 1 cut(s) | 657 |
| BtsIMutI | CAGTG | 2 cut(s) | 218, 657 |
| BveI | ACCTGC | 3 cut(s) | 199, 329, 346 |
| Cac8I | GCNNGC | 5 cut(s) | 36, 135, 284, 604, 675 |
| CaiI | CAGNNNCTG | 1 cut(s) | 659 |
| CfoI | GCGC | 4 cut(s) | 277, 608, 616, 691 |
| Cfr13I | GGNCC | 6 cut(s) | 112, 145, 199, 338, 527, 650 |
| Cfr9I | CCCGGG | 1 cut(s) | 530 |
| CseI | GACGC | 1 cut(s) | 312 |
| Csp6I | GTAC | 1 cut(s) | 359 |
| CviAII | CATG | 2 cut(s) | 674, 727 |
| CviQI | GTAC | 1 cut(s) | 359 |
| DdeI | CTNAG | 2 cut(s) | 27, 597 |
| DinI | GGCGCC | 1 cut(s) | 607 |
| DpnI | GATC | 4 cut(s) | 42, 62, 388, 668 |
| DpnII | GATC | 4 cut(s) | 40, 60, 386, 666 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 740 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 740 |
| EaeI | YGGCCR | 1 cut(s) | 213 |
| EciI | GGCGGA | 2 cut(s) | 387, 671 |
| Eco47I | GGWCC | 5 cut(s) | 112, 145, 338, 527, 650 |
| Eco57I | CTGAAG | 1 cut(s) | 275 |
| Eco88I | CYCGRG | 1 cut(s) | 530 |
| EcoO109I | RGGNCCY | 1 cut(s) | 527 |
| EcoRII | CCWGG | 3 cut(s) | 491, 519, 646 |
| EgeI | GGCGCC | 1 cut(s) | 607 |
| EheI | GGCGCC | 1 cut(s) | 607 |
| FaeI | CATG | 2 cut(s) | 677, 730 |
| FaiI | YATR | 8 cut(s) | 125, 129, 347, 482, 675, 728, 747, 749 |
| FaqI | GGGAC | 1 cut(s) | 540 |
| FatI | CATG | 2 cut(s) | 673, 726 |
| FauI | CCCGC | 2 cut(s) | 8, 517 |
| FauNDI | CATATG | 1 cut(s) | 747 |
| FbaI | TGATCA | 1 cut(s) | 60 |
| Fnu4HI | GCNGC | 2 cut(s) | 448, 724 |
| FokI | GGATG | 3 cut(s) | 503, 577, 665 |
| Fsp4HI | GCNGC | 2 cut(s) | 448, 724 |
| FspBI | CTAG | 2 cut(s) | 140, 575 |
| GlaI | GCGC | 4 cut(s) | 276, 607, 615, 690 |
| GluI | GCNGC | 2 cut(s) | 448, 724 |
| GsaI | CCCAGC | 2 cut(s) | 275, 614 |
| GsuI | CTGGAG | 1 cut(s) | 578 |
| HaeII | RGCGCY | 2 cut(s) | 609, 617 |
| HaeIII | GGCC | 4 cut(s) | 47, 194, 200, 215 |
| HapII | CCGG | 5 cut(s) | 110, 336, 374, 531, 686 |
| HgaI | GACGC | 1 cut(s) | 312 |
| HhaI | GCGC | 4 cut(s) | 277, 608, 616, 691 |
| Hin1I | GRCGYC | 1 cut(s) | 606 |
| Hin1II | CATG | 2 cut(s) | 677, 730 |
| Hin6I | GCGC | 4 cut(s) | 275, 606, 614, 689 |
| HinP1I | GCGC | 4 cut(s) | 275, 606, 614, 689 |
| HincII | GTYRAC | 3 cut(s) | 82, 88, 311 |
| HindII | GTYRAC | 3 cut(s) | 82, 88, 311 |
| HinfI | GANTC | 2 cut(s) | 21, 638 |
| HpaI | GTTAAC | 2 cut(s) | 82, 88 |
| HpaII | CCGG | 5 cut(s) | 110, 336, 374, 531, 686 |
| HphI | GGTGA | 3 cut(s) | 109, 381, 760 |
| Hpy166II | GTNNAC | 4 cut(s) | 82, 88, 95, 311 |
| Hpy188III | TCNNGA | 2 cut(s) | 228, 642 |
| Hpy8I | GTNNAC | 4 cut(s) | 82, 88, 95, 311 |
| HpyAV | CCTTC | 3 cut(s) | 158, 250, 467 |
| HpyCH4III | ACNGT | 1 cut(s) | 92 |
| HpyCH4IV | ACGT | 2 cut(s) | 84, 693 |
| HpyCH4V | TGCA | 4 cut(s) | 95, 355, 568, 673 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 44, 272, 283, 303, 361, 508 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 597 |
| HpySE526I | ACGT | 2 cut(s) | 84, 693 |
| Hsp92I | GRCGYC | 1 cut(s) | 606 |
| Hsp92II | CATG | 2 cut(s) | 677, 730 |
| HspAI | GCGC | 4 cut(s) | 275, 606, 614, 689 |
| KasI | GGCGCC | 1 cut(s) | 605 |
| KflI | GGGWCCC | 1 cut(s) | 527 |
| KpnI | GGTACC | 1 cut(s) | 362 |
| Ksp22I | TGATCA | 1 cut(s) | 60 |
| KspAI | GTTAAC | 2 cut(s) | 82, 88 |
| Kzo9I | GATC | 4 cut(s) | 40, 60, 386, 666 |
| LmnI | GCTCC | 4 cut(s) | 31, 294, 376, 559 |
| Lsp1109I | GCAGC | 1 cut(s) | 459 |
| LweI | GCATC | 1 cut(s) | 555 |
| MaeI | CTAG | 2 cut(s) | 140, 575 |
| MaeII | ACGT | 2 cut(s) | 84, 693 |
| MaeIII | GTNAC | 4 cut(s) | 429, 655, 711, 766 |
| MalI | GATC | 4 cut(s) | 42, 62, 388, 668 |
| MbiI | CCGCTC | 1 cut(s) | 562 |
| MboI | GATC | 4 cut(s) | 40, 60, 386, 666 |
| MflI | RGATCY | 1 cut(s) | 666 |
| MhlI | GDGCHC | 2 cut(s) | 97, 299 |
| MlsI | TGGCCA | 1 cut(s) | 215 |
| MluNI | TGGCCA | 1 cut(s) | 215 |
| Mly113I | GGCGCC | 1 cut(s) | 606 |
| MlyI | GAGTC | 1 cut(s) | 647 |
| MmeI | TCCRAC | 1 cut(s) | 393 |
| MnlI | CCTC | 6 cut(s) | 22, 125, 172, 190, 415, 592 |
| Mox20I | TGGCCA | 1 cut(s) | 215 |
| MscI | TGGCCA | 1 cut(s) | 215 |
| MseI | TTAA | 4 cut(s) | 81, 87, 243, 329 |
| MslI | CAYNNNNRTG | 3 cut(s) | 122, 221, 350 |
| Msp20I | TGGCCA | 1 cut(s) | 215 |
| MspA1I | CMGCKG | 3 cut(s) | 77, 364, 723 |
| MspI | CCGG | 5 cut(s) | 110, 336, 374, 531, 686 |
| MspR9I | CCNGG | 7 cut(s) | 336, 493, 521, 531, 532, 648, 686 |
| MvaI | CCWGG | 3 cut(s) | 493, 521, 648 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 44, 272, 283, 303, 361, 508 |
| NarI | GGCGCC | 1 cut(s) | 606 |
| NciI | CCSGG | 4 cut(s) | 336, 531, 532, 686 |
| NdeI | CATATG | 1 cut(s) | 747 |
| NdeII | GATC | 4 cut(s) | 40, 60, 386, 666 |
| NlaIII | CATG | 2 cut(s) | 677, 730 |
| NlaIV | GGNNCC | 4 cut(s) | 360, 528, 529, 607 |
| NmeAIII | GCCGAG | 1 cut(s) | 220 |
| NmuCI | GTSAC | 4 cut(s) | 429, 655, 711, 766 |
| NspI | RCATGY | 1 cut(s) | 677 |
| PaeI | GCATGC | 1 cut(s) | 677 |
| PaqCI | CACCTGC | 2 cut(s) | 199, 329 |
| PfeI | GAWTC | 1 cut(s) | 21 |
| PflFI | GACNNNGTC | 1 cut(s) | 306 |
| PkrI | GCNGC | 2 cut(s) | 449, 725 |
| PleI | GAGTC | 1 cut(s) | 646 |
| PluTI | GGCGCC | 1 cut(s) | 609 |
| PpsI | GAGTC | 1 cut(s) | 646 |
| Ppu21I | YACGTR | 1 cut(s) | 694 |
| PpuMI | RGGWCCY | 1 cut(s) | 527 |
| PshBI | ATTAAT | 1 cut(s) | 329 |
| Psp1406I | AACGTT | 1 cut(s) | 84 |
| Psp5II | RGGWCCY | 1 cut(s) | 527 |
| Psp6I | CCWGG | 3 cut(s) | 491, 519, 646 |
| PspFI | CCCAGC | 2 cut(s) | 271, 610 |
| PspGI | CCWGG | 3 cut(s) | 491, 519, 646 |
| PspN4I | GGNNCC | 4 cut(s) | 360, 528, 529, 607 |
| PspPI | GGNCC | 6 cut(s) | 112, 145, 199, 338, 527, 650 |
| PspPPI | RGGWCCY | 1 cut(s) | 527 |
| PstNI | CAGNNNCTG | 1 cut(s) | 659 |
| PsuI | RGATCY | 1 cut(s) | 666 |
| PsyI | GACNNNGTC | 1 cut(s) | 306 |
| RsaI | GTAC | 1 cut(s) | 360 |
| RsaNI | GTAC | 1 cut(s) | 359 |
| RseI | CAYNNNNRTG | 3 cut(s) | 122, 221, 350 |
| SaqAI | TTAA | 4 cut(s) | 81, 87, 243, 329 |
| SatI | GCNGC | 2 cut(s) | 448, 724 |
| Sau3AI | GATC | 4 cut(s) | 40, 60, 386, 666 |
| Sau96I | GGNCC | 6 cut(s) | 112, 145, 199, 338, 527, 650 |
| SchI | GAGTC | 1 cut(s) | 647 |
| ScrFI | CCNGG | 7 cut(s) | 336, 493, 521, 531, 532, 648, 686 |
| SduI | GDGCHC | 2 cut(s) | 97, 299 |
| SfaNI | GCATC | 1 cut(s) | 555 |
| SfoI | GGCGCC | 1 cut(s) | 607 |
| SinI | GGWCC | 5 cut(s) | 112, 145, 338, 527, 650 |
| SmaI | CCCGGG | 1 cut(s) | 532 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 122, 221, 350 |
| SphI | GCATGC | 1 cut(s) | 677 |
| SspDI | GGCGCC | 1 cut(s) | 605 |
| SspMI | CTAG | 2 cut(s) | 140, 575 |
| StyD4I | CCNGG | 7 cut(s) | 334, 491, 519, 529, 530, 646, 684 |
| TaaI | ACNGT | 1 cut(s) | 92 |
| TaiI | ACGT | 2 cut(s) | 87, 696 |
| TaqI | TCGA | 1 cut(s) | 452 |
| TauI | GCSGC | 1 cut(s) | 726 |
| TfiI | GAWTC | 1 cut(s) | 21 |
| Tru1I | TTAA | 4 cut(s) | 81, 87, 243, 329 |
| Tru9I | TTAA | 4 cut(s) | 81, 87, 243, 329 |
| TscAI | CASTG | 2 cut(s) | 225, 664 |
| TseFI | GTSAC | 4 cut(s) | 429, 655, 711, 766 |
| TseI | GCWGC | 1 cut(s) | 447 |
| Tsp45I | GTSAC | 4 cut(s) | 429, 655, 711, 766 |
| TspGWI | ACGGA | 1 cut(s) | 307 |
| TspMI | CCCGGG | 1 cut(s) | 530 |
| TspRI | CASTG | 2 cut(s) | 225, 664 |
| Tth111I | GACNNNGTC | 1 cut(s) | 306 |
| VneI | GTGCAC | 1 cut(s) | 93 |
| VpaK11BI | GGWCC | 5 cut(s) | 112, 145, 338, 527, 650 |
| VspI | ATTAAT | 1 cut(s) | 329 |
| XceI | RCATGY | 1 cut(s) | 677 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 499, 553 |
| XmaI | CCCGGG | 1 cut(s) | 530 |
| XspI | CTAG | 2 cut(s) | 140, 575 |