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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 6 cut(s) | 35, 74, 120, 190, 236, 385 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AclWI | GGATC | 1 cut(s) | 45 |
| AcoI | YGGCCR | 1 cut(s) | 306 |
| AfaI | GTAC | 2 cut(s) | 93, 103 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 121, 196, 413 |
| AflII | CTTAAG | 1 cut(s) | 335 |
| AgeI | ACCGGT | 1 cut(s) | 413 |
| AjnI | CCWGG | 2 cut(s) | 129, 141 |
| AluBI | AGCT | 3 cut(s) | 183, 226, 334 |
| AluI | AGCT | 3 cut(s) | 183, 226, 334 |
| Alw21I | GWGCWC | 1 cut(s) | 276 |
| AlwI | GGATC | 1 cut(s) | 45 |
| AoxI | GGCC | 2 cut(s) | 291, 306 |
| ApeKI | GCWGC | 2 cut(s) | 183, 186 |
| AsiGI | ACCGGT | 1 cut(s) | 413 |
| AspLEI | GCGC | 1 cut(s) | 370 |
| AspS9I | GGNCC | 3 cut(s) | 145, 205, 238 |
| AsuHPI | GGTGA | 1 cut(s) | 395 |
| AsuNHI | GCTAGC | 1 cut(s) | 226 |
| AvaII | GGWCC | 3 cut(s) | 145, 205, 238 |
| BaeGI | GKGCMC | 1 cut(s) | 346 |
| BalI | TGGCCA | 1 cut(s) | 308 |
| Bbv12I | GWGCWC | 1 cut(s) | 276 |
| BbvCI | CCTCAGC | 1 cut(s) | 288 |
| BbvI | GCAGC | 2 cut(s) | 170, 198 |
| BccI | CCATC | 1 cut(s) | 298 |
| BciT130I | CCWGG | 2 cut(s) | 131, 143 |
| BclI | TGATCA | 1 cut(s) | 57 |
| BfaI | CTAG | 4 cut(s) | 71, 135, 227, 233 |
| BfmI | CTRYAG | 2 cut(s) | 162, 184 |
| BfrI | CTTAAG | 1 cut(s) | 335 |
| BisI | GCNGC | 3 cut(s) | 184, 187, 190 |
| BlpI | GCTNAGC | 1 cut(s) | 174 |
| BlsI | GCNGC | 3 cut(s) | 185, 188, 191 |
| BmcAI | AGTACT | 1 cut(s) | 103 |
| Bme1390I | CCNGG | 2 cut(s) | 131, 143 |
| Bme18I | GGWCC | 3 cut(s) | 145, 205, 238 |
| BmgT120I | GGNCC | 3 cut(s) | 145, 205, 238 |
| BmiI | GGNNCC | 2 cut(s) | 126, 147 |
| BmrFI | CCNGG | 2 cut(s) | 131, 143 |
| BmrI | ACTGGG | 1 cut(s) | 47 |
| BmtI | GCTAGC | 1 cut(s) | 230 |
| BmuI | ACTGGG | 1 cut(s) | 47 |
| Bpu10I | CCTNAGC | 1 cut(s) | 288 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 174 |
| BsaJI | CCNNGG | 2 cut(s) | 129, 141 |
| BsaWI | WCCGGW | 2 cut(s) | 196, 413 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 121, 196, 413 |
| Bse118I | RCCGGY | 1 cut(s) | 413 |
| Bse1I | ACTGG | 2 cut(s) | 53, 351 |
| BseBI | CCWGG | 2 cut(s) | 131, 143 |
| BseDI | CCNNGG | 2 cut(s) | 129, 141 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 121, 196, 413 |
| BseMII | CTCAG | 3 cut(s) | 18, 188, 279 |
| BseNI | ACTGG | 2 cut(s) | 53, 351 |
| BseSI | GKGCMC | 1 cut(s) | 346 |
| BseXI | GCAGC | 2 cut(s) | 170, 198 |
| BseYI | CCCAGC | 2 cut(s) | 116, 364 |
| BsgI | GTGCAG | 1 cut(s) | 221 |
| BshFI | GGCC | 2 cut(s) | 293, 308 |
| BshTI | ACCGGT | 1 cut(s) | 413 |
| BsiHKAI | GWGCWC | 1 cut(s) | 276 |
| BsiSI | CCGG | 2 cut(s) | 197, 414 |
| BslFI | GGGAC | 1 cut(s) | 131 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 121, 196, 413 |
| BsmFI | GGGAC | 1 cut(s) | 131 |
| BsmI | GAATGC | 1 cut(s) | 266 |
| BsnI | GGCC | 2 cut(s) | 293, 308 |
| Bsp1286I | GDGCHC | 2 cut(s) | 276, 346 |
| Bsp143I | GATC | 2 cut(s) | 37, 57 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 174 |
| BspACI | CCGC | 6 cut(s) | 35, 74, 120, 190, 236, 385 |
| BspANI | GGCC | 2 cut(s) | 293, 308 |
| BspCNI | CTCAG | 3 cut(s) | 19, 187, 280 |
| BspLI | GGNNCC | 2 cut(s) | 126, 147 |
| BspMAI | CTGCAG | 1 cut(s) | 188 |
| BspOI | GCTAGC | 1 cut(s) | 230 |
| BspPI | GGATC | 1 cut(s) | 45 |
| BspTI | CTTAAG | 1 cut(s) | 335 |
| BsrFI | RCCGGY | 1 cut(s) | 413 |
| BsrI | ACTGG | 2 cut(s) | 53, 351 |
| BssAI | RCCGGY | 1 cut(s) | 413 |
| BssECI | CCNNGG | 2 cut(s) | 129, 141 |
| BssMI | GATC | 2 cut(s) | 37, 57 |
| Bst2UI | CCWGG | 2 cut(s) | 131, 143 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 89 |
| BstAFI | CTTAAG | 1 cut(s) | 335 |
| BstC8I | GCNNGC | 4 cut(s) | 33, 228, 266, 377 |
| BstDEI | CTNAG | 3 cut(s) | 27, 174, 288 |
| BstHHI | GCGC | 1 cut(s) | 370 |
| BstKTI | GATC | 2 cut(s) | 40, 60 |
| BstMBI | GATC | 2 cut(s) | 37, 57 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 189, 350, 376 |
| BstNI | CCWGG | 2 cut(s) | 131, 143 |
| BstSCI | CCNGG | 2 cut(s) | 129, 141 |
| BstSFI | CTRYAG | 2 cut(s) | 162, 184 |
| BstSLI | GKGCMC | 1 cut(s) | 346 |
| BstV1I | GCAGC | 2 cut(s) | 170, 198 |
| BsuRI | GGCC | 2 cut(s) | 293, 308 |
| BtsIMutI | CAGTG | 4 cut(s) | 24, 60, 94, 344 |
| Cac8I | GCNNGC | 4 cut(s) | 33, 228, 266, 377 |
| CfoI | GCGC | 1 cut(s) | 370 |
| Cfr10I | RCCGGY | 1 cut(s) | 413 |
| Cfr13I | GGNCC | 3 cut(s) | 145, 205, 238 |
| CseI | GACGC | 1 cut(s) | 52 |
| Csp6I | GTAC | 2 cut(s) | 92, 102 |
| CspAI | ACCGGT | 1 cut(s) | 413 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 397, 432 |
| CviQI | GTAC | 2 cut(s) | 92, 102 |
| DdeI | CTNAG | 3 cut(s) | 27, 174, 288 |
| DpnI | GATC | 2 cut(s) | 39, 59 |
| DpnII | GATC | 2 cut(s) | 37, 57 |
| EaeI | YGGCCR | 1 cut(s) | 306 |
| EciI | GGCGGA | 1 cut(s) | 50 |
| Eco47I | GGWCC | 3 cut(s) | 145, 205, 238 |
| EcoO109I | RGGNCCY | 2 cut(s) | 145, 205 |
| EcoRII | CCWGG | 2 cut(s) | 129, 141 |
| FaiI | YATR | 6 cut(s) | 218, 222, 246, 311, 440, 442 |
| FaqI | GGGAC | 1 cut(s) | 131 |
| FauI | CCCGC | 1 cut(s) | 113 |
| FbaI | TGATCA | 1 cut(s) | 57 |
| Fnu4HI | GCNGC | 3 cut(s) | 184, 187, 190 |
| Fsp4HI | GCNGC | 3 cut(s) | 184, 187, 190 |
| FspBI | CTAG | 4 cut(s) | 71, 135, 227, 233 |
| GlaI | GCGC | 1 cut(s) | 369 |
| GluI | GCNGC | 3 cut(s) | 184, 187, 190 |
| GsaI | CCCAGC | 2 cut(s) | 120, 368 |
| HaeIII | GGCC | 2 cut(s) | 293, 308 |
| HapII | CCGG | 2 cut(s) | 197, 414 |
| HgaI | GACGC | 1 cut(s) | 52 |
| HhaI | GCGC | 1 cut(s) | 370 |
| Hin6I | GCGC | 1 cut(s) | 368 |
| HinP1I | GCGC | 1 cut(s) | 368 |
| HincII | GTYRAC | 4 cut(s) | 67, 79, 85, 112 |
| HindII | GTYRAC | 4 cut(s) | 67, 79, 85, 112 |
| HpaI | GTTAAC | 2 cut(s) | 79, 85 |
| HpaII | CCGG | 2 cut(s) | 197, 414 |
| HphI | GGTGA | 1 cut(s) | 395 |
| Hpy166II | GTNNAC | 5 cut(s) | 67, 79, 85, 92, 112 |
| Hpy188III | TCNNGA | 1 cut(s) | 321 |
| Hpy8I | GTNNAC | 5 cut(s) | 67, 79, 85, 92, 112 |
| HpyAV | CCTTC | 2 cut(s) | 251, 277 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 89 |
| HpyCH4IV | ACGT | 1 cut(s) | 81 |
| HpyCH4V | TGCA | 2 cut(s) | 186, 202 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 189, 350, 376 |
| HpyF3I | CTNAG | 3 cut(s) | 27, 174, 288 |
| HpySE526I | ACGT | 1 cut(s) | 81 |
| HspAI | GCGC | 1 cut(s) | 368 |
| Ksp22I | TGATCA | 1 cut(s) | 57 |
| KspAI | GTTAAC | 2 cut(s) | 79, 85 |
| Kzo9I | GATC | 2 cut(s) | 37, 57 |
| LmnI | GCTCC | 1 cut(s) | 271 |
| Lsp1109I | GCAGC | 2 cut(s) | 170, 198 |
| MaeI | CTAG | 4 cut(s) | 71, 135, 227, 233 |
| MaeII | ACGT | 1 cut(s) | 81 |
| MaeIII | GTNAC | 1 cut(s) | 401 |
| MalI | GATC | 2 cut(s) | 39, 59 |
| MboI | GATC | 2 cut(s) | 37, 57 |
| MfeI | CAATTG | 1 cut(s) | 380 |
| MhlI | GDGCHC | 2 cut(s) | 276, 346 |
| MlsI | TGGCCA | 1 cut(s) | 308 |
| MluCI | AATT | 1 cut(s) | 380 |
| MluNI | TGGCCA | 1 cut(s) | 308 |
| MnlI | CCTC | 6 cut(s) | 160, 204, 223, 283, 333, 381 |
| Mox20I | TGGCCA | 1 cut(s) | 308 |
| MscI | TGGCCA | 1 cut(s) | 308 |
| MseI | TTAA | 3 cut(s) | 78, 84, 336 |
| MslI | CAYNNNNRTG | 1 cut(s) | 314 |
| Msp20I | TGGCCA | 1 cut(s) | 308 |
| MspA1I | CMGCKG | 1 cut(s) | 120 |
| MspCI | CTTAAG | 1 cut(s) | 335 |
| MspI | CCGG | 2 cut(s) | 197, 414 |
| MspR9I | CCNGG | 2 cut(s) | 131, 143 |
| MunI | CAATTG | 1 cut(s) | 380 |
| Mva1269I | GAATGC | 1 cut(s) | 266 |
| MvaI | CCWGG | 2 cut(s) | 131, 143 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 189, 350, 376 |
| NdeII | GATC | 2 cut(s) | 37, 57 |
| NheI | GCTAGC | 1 cut(s) | 226 |
| NlaIV | GGNNCC | 2 cut(s) | 126, 147 |
| NmuCI | GTSAC | 1 cut(s) | 401 |
| PctI | GAATGC | 1 cut(s) | 266 |
| PinAI | ACCGGT | 1 cut(s) | 413 |
| PkrI | GCNGC | 3 cut(s) | 185, 188, 191 |
| PpuMI | RGGWCCY | 2 cut(s) | 145, 205 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| Psp5II | RGGWCCY | 2 cut(s) | 145, 205 |
| Psp6I | CCWGG | 2 cut(s) | 129, 141 |
| PspFI | CCCAGC | 2 cut(s) | 116, 364 |
| PspGI | CCWGG | 2 cut(s) | 129, 141 |
| PspN4I | GGNNCC | 2 cut(s) | 126, 147 |
| PspPI | GGNCC | 3 cut(s) | 145, 205, 238 |
| PspPPI | RGGWCCY | 2 cut(s) | 145, 205 |
| PstI | CTGCAG | 1 cut(s) | 188 |
| RsaI | GTAC | 2 cut(s) | 93, 103 |
| RsaNI | GTAC | 2 cut(s) | 92, 102 |
| RseI | CAYNNNNRTG | 1 cut(s) | 314 |
| SaqAI | TTAA | 3 cut(s) | 78, 84, 336 |
| SatI | GCNGC | 3 cut(s) | 184, 187, 190 |
| Sau3AI | GATC | 2 cut(s) | 37, 57 |
| Sau96I | GGNCC | 3 cut(s) | 145, 205, 238 |
| ScaI | AGTACT | 1 cut(s) | 103 |
| ScrFI | CCNGG | 2 cut(s) | 131, 143 |
| SduI | GDGCHC | 2 cut(s) | 276, 346 |
| SfcI | CTRYAG | 2 cut(s) | 162, 184 |
| SgrAI | CRCCGGYG | 1 cut(s) | 413 |
| SinI | GGWCC | 3 cut(s) | 145, 205, 238 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 314 |
| SmlI | CTYRAG | 1 cut(s) | 335 |
| SmoI | CTYRAG | 1 cut(s) | 335 |
| Sse9I | AATT | 1 cut(s) | 380 |
| SsiI | CCGC | 6 cut(s) | 35, 74, 120, 190, 236, 385 |
| SspMI | CTAG | 4 cut(s) | 71, 135, 227, 233 |
| StyD4I | CCNGG | 2 cut(s) | 129, 141 |
| TaaI | ACNGT | 2 cut(s) | 11, 89 |
| TaiI | ACGT | 1 cut(s) | 84 |
| TasI | AATT | 1 cut(s) | 380 |
| TatI | WGTACW | 2 cut(s) | 91, 101 |
| TauI | GCSGC | 1 cut(s) | 192 |
| Tru1I | TTAA | 3 cut(s) | 78, 84, 336 |
| Tru9I | TTAA | 3 cut(s) | 78, 84, 336 |
| TscAI | CASTG | 4 cut(s) | 31, 60, 94, 351 |
| TseFI | GTSAC | 1 cut(s) | 401 |
| TseI | GCWGC | 2 cut(s) | 183, 186 |
| Tsp45I | GTSAC | 1 cut(s) | 401 |
| TspDTI | ATGAA | 1 cut(s) | 233 |
| TspRI | CASTG | 4 cut(s) | 31, 60, 94, 351 |
| Vha464I | CTTAAG | 1 cut(s) | 335 |
| VpaK11BI | GGWCC | 3 cut(s) | 145, 205, 238 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 301 |
| XspI | CTAG | 4 cut(s) | 71, 135, 227, 233 |
| ZrmI | AGTACT | 1 cut(s) | 103 |