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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 307 |
| Acc36I | ACCTGC | 1 cut(s) | 307 |
| AccB1I | GGYRCC | 1 cut(s) | 244 |
| AccBSI | CCGCTC | 1 cut(s) | 198 |
| AccII | CGCG | 2 cut(s) | 279, 400 |
| AciI | CCGC | 5 cut(s) | 35, 89, 198, 228, 400 |
| AclI | AACGTT | 1 cut(s) | 96 |
| AclWI | GGATC | 1 cut(s) | 45 |
| AcoI | YGGCCR | 1 cut(s) | 321 |
| AfaI | GTAC | 2 cut(s) | 216, 468 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 136, 262, 316, 428 |
| AflII | CTTAAG | 1 cut(s) | 350 |
| AgeI | ACCGGT | 1 cut(s) | 428 |
| AgsI | TTSAA | 1 cut(s) | 475 |
| AjnI | CCWGG | 3 cut(s) | 144, 156, 407 |
| AluBI | AGCT | 4 cut(s) | 204, 241, 349, 487 |
| AluI | AGCT | 4 cut(s) | 204, 241, 349, 487 |
| Alw21I | GWGCWC | 2 cut(s) | 109, 407 |
| Alw44I | GTGCAC | 1 cut(s) | 105 |
| AlwI | GGATC | 1 cut(s) | 45 |
| AoxI | GGCC | 4 cut(s) | 147, 300, 306, 321 |
| ApaLI | GTGCAC | 1 cut(s) | 105 |
| ApeKI | GCWGC | 2 cut(s) | 201, 204 |
| AsiGI | ACCGGT | 1 cut(s) | 428 |
| AspLEI | GCGC | 1 cut(s) | 361 |
| AspS9I | GGNCC | 5 cut(s) | 160, 220, 307, 446, 500 |
| AsuC2I | CCSGG | 2 cut(s) | 380, 444 |
| AsuHPI | GGTGA | 2 cut(s) | 217, 410 |
| AvaII | GGWCC | 4 cut(s) | 160, 220, 446, 500 |
| BaeGI | GKGCMC | 1 cut(s) | 109 |
| BalI | TGGCCA | 1 cut(s) | 323 |
| BanI | GGYRCC | 1 cut(s) | 244 |
| Bbv12I | GWGCWC | 2 cut(s) | 109, 407 |
| BbvI | GCAGC | 2 cut(s) | 191, 213 |
| BccI | CCATC | 1 cut(s) | 63 |
| BciT130I | CCWGG | 3 cut(s) | 146, 158, 409 |
| BclI | TGATCA | 1 cut(s) | 72 |
| BcnI | CCSGG | 2 cut(s) | 380, 444 |
| BfmI | CTRYAG | 1 cut(s) | 177 |
| BfoI | RGCGCY | 1 cut(s) | 362 |
| BfrI | CTTAAG | 1 cut(s) | 350 |
| BfuAI | ACCTGC | 1 cut(s) | 307 |
| BisI | GCNGC | 3 cut(s) | 199, 202, 205 |
| BlpI | GCTNAGC | 1 cut(s) | 189 |
| BlsI | GCNGC | 3 cut(s) | 200, 203, 206 |
| Bme1390I | CCNGG | 5 cut(s) | 146, 158, 380, 409, 444 |
| Bme18I | GGWCC | 4 cut(s) | 160, 220, 446, 500 |
| BmgT120I | GGNCC | 5 cut(s) | 160, 220, 307, 446, 500 |
| BmiI | GGNNCC | 4 cut(s) | 141, 162, 246, 501 |
| BmrFI | CCNGG | 5 cut(s) | 146, 158, 380, 409, 444 |
| BmrI | ACTGGG | 2 cut(s) | 36, 62 |
| BmuI | ACTGGG | 2 cut(s) | 36, 62 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 189 |
| BpuMI | CCSGG | 2 cut(s) | 380, 444 |
| BsaJI | CCNNGG | 4 cut(s) | 144, 156, 303, 408 |
| BsaWI | WCCGGW | 2 cut(s) | 211, 428 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 136, 262, 316, 428 |
| Bse118I | RCCGGY | 1 cut(s) | 428 |
| Bse1I | ACTGG | 3 cut(s) | 31, 68, 150 |
| BseBI | CCWGG | 3 cut(s) | 146, 158, 409 |
| BseDI | CCNNGG | 4 cut(s) | 144, 156, 303, 408 |
| BseGI | GGATG | 1 cut(s) | 55 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 136, 262, 316, 428 |
| BseMII | CTCAG | 1 cut(s) | 203 |
| BseNI | ACTGG | 3 cut(s) | 31, 68, 150 |
| BseSI | GKGCMC | 1 cut(s) | 109 |
| BseXI | GCAGC | 2 cut(s) | 191, 213 |
| Bsh1236I | CGCG | 2 cut(s) | 279, 400 |
| BshFI | GGCC | 4 cut(s) | 149, 302, 308, 323 |
| BshNI | GGYRCC | 1 cut(s) | 244 |
| BshTI | ACCGGT | 1 cut(s) | 428 |
| BsiHKAI | GWGCWC | 2 cut(s) | 109, 407 |
| BsiSI | CCGG | 4 cut(s) | 212, 380, 429, 444 |
| BslFI | GGGAC | 2 cut(s) | 146, 513 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 136, 262, 316, 428 |
| BsmFI | GGGAC | 2 cut(s) | 146, 513 |
| BsnI | GGCC | 4 cut(s) | 149, 302, 308, 323 |
| Bsp1286I | GDGCHC | 2 cut(s) | 109, 407 |
| Bsp143I | GATC | 2 cut(s) | 37, 72 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 189 |
| BspACI | CCGC | 5 cut(s) | 35, 89, 198, 228, 400 |
| BspANI | GGCC | 4 cut(s) | 149, 302, 308, 323 |
| BspCNI | CTCAG | 1 cut(s) | 202 |
| BspFNI | CGCG | 2 cut(s) | 279, 400 |
| BspLI | GGNNCC | 4 cut(s) | 141, 162, 246, 501 |
| BspMI | ACCTGC | 1 cut(s) | 307 |
| BspPI | GGATC | 1 cut(s) | 45 |
| BspT107I | GGYRCC | 1 cut(s) | 244 |
| BspTI | CTTAAG | 1 cut(s) | 350 |
| BsrBI | CCGCTC | 1 cut(s) | 198 |
| BsrFI | RCCGGY | 1 cut(s) | 428 |
| BsrI | ACTGG | 3 cut(s) | 31, 68, 150 |
| BssAI | RCCGGY | 1 cut(s) | 428 |
| BssECI | CCNNGG | 4 cut(s) | 144, 156, 303, 408 |
| BssMI | GATC | 2 cut(s) | 37, 72 |
| Bst2UI | CCWGG | 3 cut(s) | 146, 158, 409 |
| Bst4CI | ACNGT | 2 cut(s) | 104, 471 |
| BstAFI | CTTAAG | 1 cut(s) | 350 |
| BstC8I | GCNNGC | 4 cut(s) | 33, 243, 281, 392 |
| BstDEI | CTNAG | 1 cut(s) | 189 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 260 |
| BstF5I | GGATG | 1 cut(s) | 55 |
| BstFNI | CGCG | 2 cut(s) | 279, 400 |
| BstH2I | RGCGCY | 1 cut(s) | 362 |
| BstHHI | GCGC | 1 cut(s) | 361 |
| BstKTI | GATC | 2 cut(s) | 40, 75 |
| BstMBI | GATC | 2 cut(s) | 37, 72 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 204, 365, 380, 411 |
| BstNI | CCWGG | 3 cut(s) | 146, 158, 409 |
| BstSCI | CCNGG | 5 cut(s) | 144, 156, 378, 407, 442 |
| BstSFI | CTRYAG | 1 cut(s) | 177 |
| BstSLI | GKGCMC | 1 cut(s) | 109 |
| BstUI | CGCG | 2 cut(s) | 279, 400 |
| BstV1I | GCAGC | 2 cut(s) | 191, 213 |
| BsuRI | GGCC | 4 cut(s) | 149, 302, 308, 323 |
| BtsCI | GGATG | 1 cut(s) | 55 |
| BtsIMutI | CAGTG | 3 cut(s) | 24, 75, 326 |
| BveI | ACCTGC | 1 cut(s) | 307 |
| Cac8I | GCNNGC | 4 cut(s) | 33, 243, 281, 392 |
| CfoI | GCGC | 1 cut(s) | 361 |
| Cfr10I | RCCGGY | 1 cut(s) | 428 |
| Cfr13I | GGNCC | 5 cut(s) | 160, 220, 307, 446, 500 |
| Csp6I | GTAC | 2 cut(s) | 215, 467 |
| CspAI | ACCGGT | 1 cut(s) | 428 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 412, 447 |
| CviAII | CATG | 1 cut(s) | 454 |
| CviQI | GTAC | 2 cut(s) | 215, 467 |
| DdeI | CTNAG | 1 cut(s) | 189 |
| DpnI | GATC | 2 cut(s) | 39, 74 |
| DpnII | GATC | 2 cut(s) | 37, 72 |
| EaeI | YGGCCR | 1 cut(s) | 321 |
| EciI | GGCGGA | 1 cut(s) | 50 |
| Eco47I | GGWCC | 4 cut(s) | 160, 220, 446, 500 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 260 |
| EcoO109I | RGGNCCY | 2 cut(s) | 160, 220 |
| EcoRII | CCWGG | 3 cut(s) | 144, 156, 407 |
| FaeI | CATG | 1 cut(s) | 457 |
| FaiI | YATR | 4 cut(s) | 179, 233, 237, 455 |
| FaqI | GGGAC | 2 cut(s) | 146, 513 |
| FatI | CATG | 1 cut(s) | 453 |
| FbaI | TGATCA | 1 cut(s) | 72 |
| Fnu4HI | GCNGC | 3 cut(s) | 199, 202, 205 |
| FokI | GGATG | 1 cut(s) | 42 |
| Fsp4HI | GCNGC | 3 cut(s) | 199, 202, 205 |
| GlaI | GCGC | 1 cut(s) | 360 |
| GluI | GCNGC | 3 cut(s) | 199, 202, 205 |
| HaeII | RGCGCY | 1 cut(s) | 362 |
| HaeIII | GGCC | 4 cut(s) | 149, 302, 308, 323 |
| HapII | CCGG | 4 cut(s) | 212, 380, 429, 444 |
| HhaI | GCGC | 1 cut(s) | 361 |
| Hin1II | CATG | 1 cut(s) | 457 |
| Hin6I | GCGC | 1 cut(s) | 359 |
| HinP1I | GCGC | 1 cut(s) | 359 |
| HincII | GTYRAC | 3 cut(s) | 94, 100, 127 |
| HindII | GTYRAC | 3 cut(s) | 94, 100, 127 |
| HpaI | GTTAAC | 2 cut(s) | 94, 100 |
| HpaII | CCGG | 4 cut(s) | 212, 380, 429, 444 |
| HphI | GGTGA | 2 cut(s) | 217, 410 |
| Hpy166II | GTNNAC | 4 cut(s) | 94, 100, 107, 127 |
| Hpy188I | TCNGA | 1 cut(s) | 116 |
| Hpy188III | TCNNGA | 2 cut(s) | 336, 509 |
| Hpy8I | GTNNAC | 4 cut(s) | 94, 100, 107, 127 |
| Hpy99I | CGWCG | 1 cut(s) | 124 |
| HpyAV | CCTTC | 1 cut(s) | 266 |
| HpyCH4III | ACNGT | 2 cut(s) | 104, 471 |
| HpyCH4IV | ACGT | 2 cut(s) | 96, 119 |
| HpyCH4V | TGCA | 3 cut(s) | 107, 457, 463 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 204, 365, 380, 411 |
| HpyF3I | CTNAG | 1 cut(s) | 189 |
| HpySE526I | ACGT | 2 cut(s) | 96, 119 |
| Hsp92II | CATG | 1 cut(s) | 457 |
| HspAI | GCGC | 1 cut(s) | 359 |
| Ksp22I | TGATCA | 1 cut(s) | 72 |
| KspAI | GTTAAC | 2 cut(s) | 94, 100 |
| Kzo9I | GATC | 2 cut(s) | 37, 72 |
| LmnI | GCTCC | 2 cut(s) | 286, 402 |
| Lsp1109I | GCAGC | 2 cut(s) | 191, 213 |
| MaeII | ACGT | 2 cut(s) | 96, 119 |
| MaeIII | GTNAC | 1 cut(s) | 416 |
| MalI | GATC | 2 cut(s) | 39, 74 |
| MbiI | CCGCTC | 1 cut(s) | 198 |
| MboI | GATC | 2 cut(s) | 37, 72 |
| MhlI | GDGCHC | 2 cut(s) | 109, 407 |
| MlsI | TGGCCA | 1 cut(s) | 323 |
| MluNI | TGGCCA | 1 cut(s) | 323 |
| MnlI | CCTC | 6 cut(s) | 178, 219, 233, 298, 348, 485 |
| Mox20I | TGGCCA | 1 cut(s) | 323 |
| MscI | TGGCCA | 1 cut(s) | 323 |
| MseI | TTAA | 3 cut(s) | 93, 99, 351 |
| MslI | CAYNNNNRTG | 3 cut(s) | 230, 329, 458 |
| Msp20I | TGGCCA | 1 cut(s) | 323 |
| MspA1I | CMGCKG | 2 cut(s) | 89, 204 |
| MspCI | CTTAAG | 1 cut(s) | 350 |
| MspI | CCGG | 4 cut(s) | 212, 380, 429, 444 |
| MspR9I | CCNGG | 5 cut(s) | 146, 158, 380, 409, 444 |
| MvaI | CCWGG | 3 cut(s) | 146, 158, 409 |
| MvnI | CGCG | 2 cut(s) | 279, 400 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 204, 365, 380, 411 |
| NciI | CCSGG | 2 cut(s) | 380, 444 |
| NdeII | GATC | 2 cut(s) | 37, 72 |
| NlaIII | CATG | 1 cut(s) | 457 |
| NlaIV | GGNNCC | 4 cut(s) | 141, 162, 246, 501 |
| NmeAIII | GCCGAG | 2 cut(s) | 219, 328 |
| NmuCI | GTSAC | 1 cut(s) | 416 |
| PaqCI | CACCTGC | 1 cut(s) | 307 |
| PinAI | ACCGGT | 1 cut(s) | 428 |
| PkrI | GCNGC | 3 cut(s) | 200, 203, 206 |
| PpuMI | RGGWCCY | 2 cut(s) | 160, 220 |
| Psp1406I | AACGTT | 1 cut(s) | 96 |
| Psp5II | RGGWCCY | 2 cut(s) | 160, 220 |
| Psp6I | CCWGG | 3 cut(s) | 144, 156, 407 |
| PspGI | CCWGG | 3 cut(s) | 144, 156, 407 |
| PspN4I | GGNNCC | 4 cut(s) | 141, 162, 246, 501 |
| PspPI | GGNCC | 5 cut(s) | 160, 220, 307, 446, 500 |
| PspPPI | RGGWCCY | 2 cut(s) | 160, 220 |
| PvuII | CAGCTG | 1 cut(s) | 204 |
| RsaI | GTAC | 2 cut(s) | 216, 468 |
| RsaNI | GTAC | 2 cut(s) | 215, 467 |
| RseI | CAYNNNNRTG | 3 cut(s) | 230, 329, 458 |
| SaqAI | TTAA | 3 cut(s) | 93, 99, 351 |
| SatI | GCNGC | 3 cut(s) | 199, 202, 205 |
| Sau3AI | GATC | 2 cut(s) | 37, 72 |
| Sau96I | GGNCC | 5 cut(s) | 160, 220, 307, 446, 500 |
| ScrFI | CCNGG | 5 cut(s) | 146, 158, 380, 409, 444 |
| SduI | GDGCHC | 2 cut(s) | 109, 407 |
| SfcI | CTRYAG | 1 cut(s) | 177 |
| SgrAI | CRCCGGYG | 1 cut(s) | 428 |
| SinI | GGWCC | 4 cut(s) | 160, 220, 446, 500 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 230, 329, 458 |
| SmlI | CTYRAG | 1 cut(s) | 350 |
| SmoI | CTYRAG | 1 cut(s) | 350 |
| SsiI | CCGC | 5 cut(s) | 35, 89, 198, 228, 400 |
| StyD4I | CCNGG | 5 cut(s) | 144, 156, 378, 407, 442 |
| TaaI | ACNGT | 2 cut(s) | 104, 471 |
| TaiI | ACGT | 2 cut(s) | 99, 122 |
| TaqII | GACCGA | 1 cut(s) | 112 |
| TauI | GCSGC | 1 cut(s) | 201 |
| Tru1I | TTAA | 3 cut(s) | 93, 99, 351 |
| Tru9I | TTAA | 3 cut(s) | 93, 99, 351 |
| TscAI | CASTG | 3 cut(s) | 31, 75, 333 |
| TseFI | GTSAC | 1 cut(s) | 416 |
| TseI | GCWGC | 2 cut(s) | 201, 204 |
| Tsp45I | GTSAC | 1 cut(s) | 416 |
| TspRI | CASTG | 3 cut(s) | 31, 75, 333 |
| Vha464I | CTTAAG | 1 cut(s) | 350 |
| VneI | GTGCAC | 1 cut(s) | 105 |
| VpaK11BI | GGWCC | 4 cut(s) | 160, 220, 446, 500 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 260 |