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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 392 |
| Acc36I | ACCTGC | 1 cut(s) | 392 |
| AccB1I | GGYRCC | 2 cut(s) | 136, 404 |
| AccBSI | CCGCTC | 1 cut(s) | 238 |
| AccII | CGCG | 1 cut(s) | 48 |
| AciI | CCGC | 4 cut(s) | 236, 252, 295, 330 |
| AcoI | YGGCCR | 1 cut(s) | 90 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 258, 290, 397 |
| AflIII | ACRYGT | 1 cut(s) | 85 |
| AjnI | CCWGG | 3 cut(s) | 257, 318, 445 |
| AluBI | AGCT | 3 cut(s) | 10, 301, 469 |
| AluI | AGCT | 3 cut(s) | 10, 301, 469 |
| Alw21I | GWGCWC | 2 cut(s) | 60, 291 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 373 |
| Ama87I | CYCGRG | 1 cut(s) | 290 |
| AoxI | GGCC | 4 cut(s) | 75, 90, 261, 449 |
| ApaI | GGGCCC | 1 cut(s) | 453 |
| ApeKI | GCWGC | 1 cut(s) | 367 |
| AspLEI | GCGC | 2 cut(s) | 48, 415 |
| AspS9I | GGNCC | 6 cut(s) | 76, 191, 261, 326, 449, 450 |
| AsuC2I | CCSGG | 1 cut(s) | 392 |
| AsuHPI | GGTGA | 1 cut(s) | 232 |
| AvaI | CYCGRG | 1 cut(s) | 290 |
| AvaII | GGWCC | 2 cut(s) | 191, 326 |
| BaeGI | GKGCMC | 2 cut(s) | 409, 453 |
| BalI | TGGCCA | 1 cut(s) | 92 |
| BanI | GGYRCC | 2 cut(s) | 136, 404 |
| BanII | GRGCYC | 1 cut(s) | 453 |
| Bbv12I | GWGCWC | 2 cut(s) | 60, 291 |
| BbvI | GCAGC | 1 cut(s) | 379 |
| BciT130I | CCWGG | 3 cut(s) | 259, 320, 447 |
| BcnI | CCSGG | 1 cut(s) | 392 |
| BfaI | CTAG | 1 cut(s) | 523 |
| BfmI | CTRYAG | 2 cut(s) | 131, 146 |
| BfoI | RGCGCY | 1 cut(s) | 416 |
| BfuAI | ACCTGC | 1 cut(s) | 392 |
| BglII | AGATCT | 1 cut(s) | 487 |
| BisI | GCNGC | 1 cut(s) | 368 |
| BlsI | GCNGC | 1 cut(s) | 369 |
| Bme1390I | CCNGG | 4 cut(s) | 259, 320, 392, 447 |
| Bme18I | GGWCC | 2 cut(s) | 191, 326 |
| BmeT110I | CYCGRG | 1 cut(s) | 290 |
| BmgT120I | GGNCC | 6 cut(s) | 76, 191, 261, 326, 449, 450 |
| BmiI | GGNNCC | 5 cut(s) | 138, 192, 328, 406, 451 |
| BmrFI | CCNGG | 4 cut(s) | 259, 320, 392, 447 |
| BmsI | GCATC | 2 cut(s) | 141, 446 |
| BpmI | CTGGAG | 1 cut(s) | 302 |
| BpuMI | CCSGG | 1 cut(s) | 392 |
| BsaJI | CCNNGG | 2 cut(s) | 258, 446 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 258, 290, 397 |
| Bse1I | ACTGG | 2 cut(s) | 105, 378 |
| BseBI | CCWGG | 3 cut(s) | 259, 320, 447 |
| BseDI | CCNNGG | 2 cut(s) | 258, 446 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 258, 290, 397 |
| BseNI | ACTGG | 2 cut(s) | 105, 378 |
| BseRI | GAGGAG | 1 cut(s) | 498 |
| BseSI | GKGCMC | 2 cut(s) | 409, 453 |
| BseXI | GCAGC | 1 cut(s) | 379 |
| BseYI | CCCAGC | 1 cut(s) | 409 |
| BsgI | GTGCAG | 1 cut(s) | 386 |
| Bsh1236I | CGCG | 1 cut(s) | 48 |
| BshFI | GGCC | 4 cut(s) | 77, 92, 263, 451 |
| BshNI | GGYRCC | 2 cut(s) | 136, 404 |
| BsiHKAI | GWGCWC | 2 cut(s) | 60, 291 |
| BsiHKCI | CYCGRG | 1 cut(s) | 290 |
| BsiSI | CCGG | 1 cut(s) | 391 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 258, 290, 397 |
| BsnI | GGCC | 4 cut(s) | 77, 92, 263, 451 |
| BsoBI | CYCGRG | 1 cut(s) | 290 |
| Bsp120I | GGGCCC | 1 cut(s) | 449 |
| Bsp1286I | GDGCHC | 4 cut(s) | 60, 291, 409, 453 |
| Bsp143I | GATC | 1 cut(s) | 487 |
| BspACI | CCGC | 4 cut(s) | 236, 252, 295, 330 |
| BspANI | GGCC | 4 cut(s) | 77, 92, 263, 451 |
| BspFNI | CGCG | 1 cut(s) | 48 |
| BspLI | GGNNCC | 5 cut(s) | 138, 192, 328, 406, 451 |
| BspMAI | CTGCAG | 1 cut(s) | 150 |
| BspMI | ACCTGC | 1 cut(s) | 392 |
| BspT107I | GGYRCC | 2 cut(s) | 136, 404 |
| BsrBI | CCGCTC | 1 cut(s) | 238 |
| BsrI | ACTGG | 2 cut(s) | 105, 378 |
| BssECI | CCNNGG | 2 cut(s) | 258, 446 |
| BssMI | GATC | 1 cut(s) | 487 |
| Bst2UI | CCWGG | 3 cut(s) | 259, 320, 447 |
| BstC8I | GCNNGC | 2 cut(s) | 12, 303 |
| BstFNI | CGCG | 1 cut(s) | 48 |
| BstH2I | RGCGCY | 1 cut(s) | 416 |
| BstHHI | GCGC | 2 cut(s) | 48, 415 |
| BstKTI | GATC | 1 cut(s) | 490 |
| BstMBI | GATC | 1 cut(s) | 487 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 260, 307, 329, 338, 457, 466 |
| BstNI | CCWGG | 3 cut(s) | 259, 320, 447 |
| BstNSI | RCATGY | 1 cut(s) | 89 |
| BstSCI | CCNGG | 4 cut(s) | 257, 318, 390, 445 |
| BstSFI | CTRYAG | 2 cut(s) | 131, 146 |
| BstSLI | GKGCMC | 2 cut(s) | 409, 453 |
| BstUI | CGCG | 1 cut(s) | 48 |
| BstV1I | GCAGC | 1 cut(s) | 379 |
| BstX2I | RGATCY | 1 cut(s) | 487 |
| BstYI | RGATCY | 1 cut(s) | 487 |
| BsuRI | GGCC | 4 cut(s) | 77, 92, 263, 451 |
| BveI | ACCTGC | 1 cut(s) | 392 |
| Cac8I | GCNNGC | 2 cut(s) | 12, 303 |
| CaiI | CAGNNNCTG | 1 cut(s) | 373 |
| CfoI | GCGC | 2 cut(s) | 48, 415 |
| Cfr13I | GGNCC | 6 cut(s) | 76, 191, 261, 326, 449, 450 |
| CviAII | CATG | 1 cut(s) | 86 |
| CviJI | RGCY | 9 cut(s) | 10, 77, 92, 263, 301, 347, 385, 451, 469 |
| CviKI_1 | RGCY | 9 cut(s) | 10, 77, 92, 263, 301, 347, 385, 451, 469 |
| DpnI | GATC | 1 cut(s) | 489 |
| DpnII | GATC | 1 cut(s) | 487 |
| EaeI | YGGCCR | 1 cut(s) | 90 |
| Eco24I | GRGCYC | 1 cut(s) | 453 |
| Eco47I | GGWCC | 2 cut(s) | 191, 326 |
| Eco88I | CYCGRG | 1 cut(s) | 290 |
| EcoO109I | RGGNCCY | 2 cut(s) | 326, 449 |
| EcoRII | CCWGG | 3 cut(s) | 257, 318, 445 |
| EcoT38I | GRGCYC | 1 cut(s) | 453 |
| FaeI | CATG | 1 cut(s) | 89 |
| FaiI | YATR | 4 cut(s) | 6, 87, 458, 516 |
| FatI | CATG | 1 cut(s) | 85 |
| FauI | CCCGC | 2 cut(s) | 288, 337 |
| Fnu4HI | GCNGC | 1 cut(s) | 368 |
| FriOI | GRGCYC | 1 cut(s) | 453 |
| Fsp4HI | GCNGC | 1 cut(s) | 368 |
| FspBI | CTAG | 1 cut(s) | 523 |
| GlaI | GCGC | 2 cut(s) | 47, 414 |
| GluI | GCNGC | 1 cut(s) | 368 |
| GsaI | CCCAGC | 1 cut(s) | 413 |
| GsuI | CTGGAG | 1 cut(s) | 302 |
| HaeII | RGCGCY | 1 cut(s) | 416 |
| HaeIII | GGCC | 4 cut(s) | 77, 92, 263, 451 |
| HapII | CCGG | 1 cut(s) | 391 |
| HhaI | GCGC | 2 cut(s) | 48, 415 |
| Hin1II | CATG | 1 cut(s) | 89 |
| Hin6I | GCGC | 2 cut(s) | 46, 413 |
| HinP1I | GCGC | 2 cut(s) | 46, 413 |
| HincII | GTYRAC | 1 cut(s) | 110 |
| HindII | GTYRAC | 1 cut(s) | 110 |
| HinfI | GANTC | 3 cut(s) | 22, 221, 227 |
| HpaII | CCGG | 1 cut(s) | 391 |
| HphI | GGTGA | 1 cut(s) | 232 |
| Hpy166II | GTNNAC | 2 cut(s) | 110, 194 |
| Hpy188I | TCNGA | 2 cut(s) | 226, 499 |
| Hpy188III | TCNNGA | 1 cut(s) | 441 |
| Hpy8I | GTNNAC | 2 cut(s) | 110, 194 |
| HpyAV | CCTTC | 1 cut(s) | 61 |
| HpyCH4V | TGCA | 5 cut(s) | 148, 154, 341, 367, 437 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 260, 307, 329, 338, 457, 466 |
| Hsp92II | CATG | 1 cut(s) | 89 |
| HspAI | GCGC | 2 cut(s) | 46, 413 |
| Kzo9I | GATC | 1 cut(s) | 487 |
| LmnI | GCTCC | 3 cut(s) | 55, 254, 298 |
| Lsp1109I | GCAGC | 1 cut(s) | 379 |
| LweI | GCATC | 2 cut(s) | 141, 446 |
| MaeI | CTAG | 1 cut(s) | 523 |
| MalI | GATC | 1 cut(s) | 489 |
| MbiI | CCGCTC | 1 cut(s) | 238 |
| MboI | GATC | 1 cut(s) | 487 |
| MflI | RGATCY | 1 cut(s) | 487 |
| MhlI | GDGCHC | 4 cut(s) | 60, 291, 409, 453 |
| MlsI | TGGCCA | 1 cut(s) | 92 |
| MluCI | AATT | 2 cut(s) | 351, 511 |
| MluNI | TGGCCA | 1 cut(s) | 92 |
| MlyI | GAGTC | 1 cut(s) | 236 |
| MnlI | CCTC | 4 cut(s) | 67, 207, 326, 476 |
| Mox20I | TGGCCA | 1 cut(s) | 92 |
| MscI | TGGCCA | 1 cut(s) | 92 |
| MslI | CAYNNNNRTG | 1 cut(s) | 98 |
| Msp20I | TGGCCA | 1 cut(s) | 92 |
| MspI | CCGG | 1 cut(s) | 391 |
| MspR9I | CCNGG | 4 cut(s) | 259, 320, 392, 447 |
| MvaI | CCWGG | 3 cut(s) | 259, 320, 447 |
| MvnI | CGCG | 1 cut(s) | 48 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 260, 307, 329, 338, 457, 466 |
| NciI | CCSGG | 1 cut(s) | 392 |
| NdeII | GATC | 1 cut(s) | 487 |
| NlaIII | CATG | 1 cut(s) | 89 |
| NlaIV | GGNNCC | 5 cut(s) | 138, 192, 328, 406, 451 |
| NspI | RCATGY | 1 cut(s) | 89 |
| PaqCI | CACCTGC | 1 cut(s) | 392 |
| PciI | ACATGT | 1 cut(s) | 85 |
| PfeI | GAWTC | 2 cut(s) | 22, 221 |
| PkrI | GCNGC | 1 cut(s) | 369 |
| PleI | GAGTC | 1 cut(s) | 235 |
| PpsI | GAGTC | 1 cut(s) | 235 |
| PpuMI | RGGWCCY | 1 cut(s) | 326 |
| PscI | ACATGT | 1 cut(s) | 85 |
| Psp5II | RGGWCCY | 1 cut(s) | 326 |
| Psp6I | CCWGG | 3 cut(s) | 257, 318, 445 |
| PspFI | CCCAGC | 1 cut(s) | 409 |
| PspGI | CCWGG | 3 cut(s) | 257, 318, 445 |
| PspN4I | GGNNCC | 5 cut(s) | 138, 192, 328, 406, 451 |
| PspOMI | GGGCCC | 1 cut(s) | 449 |
| PspPI | GGNCC | 6 cut(s) | 76, 191, 261, 326, 449, 450 |
| PspPPI | RGGWCCY | 1 cut(s) | 326 |
| PstI | CTGCAG | 1 cut(s) | 150 |
| PstNI | CAGNNNCTG | 1 cut(s) | 373 |
| PsuI | RGATCY | 1 cut(s) | 487 |
| RseI | CAYNNNNRTG | 1 cut(s) | 98 |
| SatI | GCNGC | 1 cut(s) | 368 |
| Sau3AI | GATC | 1 cut(s) | 487 |
| Sau96I | GGNCC | 6 cut(s) | 76, 191, 261, 326, 449, 450 |
| SchI | GAGTC | 1 cut(s) | 236 |
| ScrFI | CCNGG | 4 cut(s) | 259, 320, 392, 447 |
| SduI | GDGCHC | 4 cut(s) | 60, 291, 409, 453 |
| SetI | ASST | 8 cut(s) | 12, 72, 199, 286, 303, 318, 406, 471 |
| SfaNI | GCATC | 2 cut(s) | 141, 446 |
| SfcI | CTRYAG | 2 cut(s) | 131, 146 |
| SinI | GGWCC | 2 cut(s) | 191, 326 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 98 |
| Sse9I | AATT | 2 cut(s) | 351, 511 |
| SsiI | CCGC | 4 cut(s) | 236, 252, 295, 330 |
| SspMI | CTAG | 1 cut(s) | 523 |
| StyD4I | CCNGG | 4 cut(s) | 257, 318, 390, 445 |
| TaqI | TCGA | 1 cut(s) | 72 |
| TasI | AATT | 2 cut(s) | 351, 511 |
| TfiI | GAWTC | 2 cut(s) | 22, 221 |
| TseI | GCWGC | 1 cut(s) | 367 |
| VpaK11BI | GGWCC | 2 cut(s) | 191, 326 |
| XceI | RCATGY | 1 cut(s) | 89 |
| XspI | CTAG | 1 cut(s) | 523 |