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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 18 |
| AcoI | YGGCCR | 2 cut(s) | 205, 609 |
| AcsI | RAATTY | 1 cut(s) | 50 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 214, 253, 424, 550 |
| AgeI | ACCGGT | 1 cut(s) | 83 |
| AgsI | TTSAA | 3 cut(s) | 104, 403, 593 |
| AjnI | CCWGG | 2 cut(s) | 109, 444 |
| AluBI | AGCT | 3 cut(s) | 221, 423, 565 |
| AluI | AGCT | 3 cut(s) | 221, 423, 565 |
| Alw21I | GWGCWC | 2 cut(s) | 397, 567 |
| Alw26I | GTCTC | 1 cut(s) | 467 |
| Alw44I | GTGCAC | 1 cut(s) | 393 |
| AlwI | GGATC | 1 cut(s) | 18 |
| AoxI | GGCC | 3 cut(s) | 205, 502, 609 |
| ApaLI | GTGCAC | 1 cut(s) | 393 |
| ApeKI | GCWGC | 2 cut(s) | 5, 486 |
| ApoI | RAATTY | 1 cut(s) | 50 |
| AsiGI | ACCGGT | 1 cut(s) | 83 |
| AspLEI | GCGC | 2 cut(s) | 264, 579 |
| AspS9I | GGNCC | 5 cut(s) | 39, 256, 325, 448, 541 |
| AsuNHI | GCTAGC | 1 cut(s) | 362 |
| AvaII | GGWCC | 5 cut(s) | 39, 256, 325, 448, 541 |
| AxyI | CCTNAGG | 1 cut(s) | 432 |
| BaeGI | GKGCMC | 1 cut(s) | 397 |
| BalI | TGGCCA | 1 cut(s) | 611 |
| BanII | GRGCYC | 1 cut(s) | 567 |
| Bbv12I | GWGCWC | 2 cut(s) | 397, 567 |
| BbvI | GCAGC | 2 cut(s) | 17, 498 |
| BccI | CCATC | 3 cut(s) | 146, 273, 351 |
| BciT130I | CCWGG | 2 cut(s) | 111, 446 |
| BclI | TGATCA | 1 cut(s) | 124 |
| BcoDI | GTCTC | 1 cut(s) | 467 |
| BfaI | CTAG | 3 cut(s) | 363, 420, 482 |
| BfoI | RGCGCY | 1 cut(s) | 265 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 501 |
| BisI | GCNGC | 4 cut(s) | 6, 75, 487, 516 |
| BlsI | GCNGC | 4 cut(s) | 7, 76, 488, 517 |
| Bme1390I | CCNGG | 2 cut(s) | 111, 446 |
| Bme18I | GGWCC | 5 cut(s) | 39, 256, 325, 448, 541 |
| BmgT120I | GGNCC | 5 cut(s) | 39, 256, 325, 448, 541 |
| BmiI | GGNNCC | 3 cut(s) | 41, 429, 450 |
| BmrFI | CCNGG | 2 cut(s) | 111, 446 |
| BmtI | GCTAGC | 1 cut(s) | 366 |
| BpmI | CTGGAG | 1 cut(s) | 93 |
| BsaI | GGTCTC | 1 cut(s) | 467 |
| BsaJI | CCNNGG | 2 cut(s) | 357, 444 |
| BsaWI | WCCGGW | 2 cut(s) | 83, 253 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 214, 253, 424, 550 |
| Bse118I | RCCGGY | 1 cut(s) | 83 |
| Bse1I | ACTGG | 1 cut(s) | 410 |
| Bse21I | CCTNAGG | 1 cut(s) | 432 |
| BseBI | CCWGG | 2 cut(s) | 111, 446 |
| BseDI | CCNNGG | 2 cut(s) | 357, 444 |
| BseGI | GGATG | 3 cut(s) | 157, 265, 343 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 214, 253, 424, 550 |
| BseMII | CTCAG | 1 cut(s) | 446 |
| BseNI | ACTGG | 1 cut(s) | 410 |
| BseRI | GAGGAG | 2 cut(s) | 48, 51 |
| BseSI | GKGCMC | 1 cut(s) | 397 |
| BseXI | GCAGC | 2 cut(s) | 17, 498 |
| BshFI | GGCC | 3 cut(s) | 207, 504, 611 |
| BshTI | ACCGGT | 1 cut(s) | 83 |
| BsiHKAI | GWGCWC | 2 cut(s) | 397, 567 |
| BsiSI | CCGG | 3 cut(s) | 84, 204, 254 |
| BslFI | GGGAC | 1 cut(s) | 434 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 214, 253, 424, 550 |
| BsmAI | GTCTC | 1 cut(s) | 467 |
| BsmFI | GGGAC | 1 cut(s) | 434 |
| BsnI | GGCC | 3 cut(s) | 207, 504, 611 |
| Bso31I | GGTCTC | 1 cut(s) | 467 |
| Bsp1286I | GDGCHC | 2 cut(s) | 397, 567 |
| Bsp143I | GATC | 2 cut(s) | 10, 124 |
| BspANI | GGCC | 3 cut(s) | 207, 504, 611 |
| BspCNI | CTCAG | 1 cut(s) | 445 |
| BspLI | GGNNCC | 3 cut(s) | 41, 429, 450 |
| BspOI | GCTAGC | 1 cut(s) | 366 |
| BspPI | GGATC | 1 cut(s) | 18 |
| BspQI | GCTCTTC | 1 cut(s) | 309 |
| BspTNI | GGTCTC | 1 cut(s) | 467 |
| BsrFI | RCCGGY | 1 cut(s) | 83 |
| BsrI | ACTGG | 1 cut(s) | 410 |
| BssAI | RCCGGY | 1 cut(s) | 83 |
| BssECI | CCNNGG | 2 cut(s) | 357, 444 |
| BssMI | GATC | 2 cut(s) | 10, 124 |
| Bst2UI | CCWGG | 2 cut(s) | 111, 446 |
| Bst4CI | ACNGT | 3 cut(s) | 296, 379, 392 |
| Bst6I | CTCTTC | 1 cut(s) | 309 |
| BstC8I | GCNNGC | 2 cut(s) | 321, 364 |
| BstDEI | CTNAG | 1 cut(s) | 432 |
| BstDSI | CCRYGG | 1 cut(s) | 357 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 548 |
| BstF5I | GGATG | 3 cut(s) | 157, 265, 343 |
| BstH2I | RGCGCY | 1 cut(s) | 265 |
| BstHHI | GCGC | 2 cut(s) | 264, 579 |
| BstKTI | GATC | 2 cut(s) | 13, 127 |
| BstMAI | GTCTC | 1 cut(s) | 467 |
| BstMBI | GATC | 2 cut(s) | 10, 124 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 492, 501 |
| BstNI | CCWGG | 2 cut(s) | 111, 446 |
| BstSCI | CCNGG | 2 cut(s) | 109, 444 |
| BstSLI | GKGCMC | 1 cut(s) | 397 |
| BstV1I | GCAGC | 2 cut(s) | 17, 498 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 358 |
| Bsu36I | CCTNAGG | 1 cut(s) | 432 |
| BsuRI | GGCC | 3 cut(s) | 207, 504, 611 |
| BtgI | CCRYGG | 1 cut(s) | 357 |
| BtsCI | GGATG | 3 cut(s) | 157, 265, 343 |
| BtsIMutI | CAGTG | 1 cut(s) | 452 |
| Cac8I | GCNNGC | 2 cut(s) | 321, 364 |
| CfoI | GCGC | 2 cut(s) | 264, 579 |
| Cfr10I | RCCGGY | 1 cut(s) | 83 |
| Cfr13I | GGNCC | 5 cut(s) | 39, 256, 325, 448, 541 |
| CspAI | ACCGGT | 1 cut(s) | 83 |
| CviAII | CATG | 3 cut(s) | 192, 209, 286 |
| CviJI | RGCY | 9 cut(s) | 77, 207, 221, 362, 423, 504, 565, 596, 611 |
| CviKI_1 | RGCY | 9 cut(s) | 77, 207, 221, 362, 423, 504, 565, 596, 611 |
| DdeI | CTNAG | 1 cut(s) | 432 |
| DpnI | GATC | 2 cut(s) | 12, 126 |
| DpnII | GATC | 2 cut(s) | 10, 124 |
| EaeI | YGGCCR | 2 cut(s) | 205, 609 |
| Eam1104I | CTCTTC | 1 cut(s) | 309 |
| EarI | CTCTTC | 1 cut(s) | 309 |
| EciI | GGCGGA | 5 cut(s) | 211, 236, 322, 338, 554 |
| Ecl136II | GAGCTC | 1 cut(s) | 565 |
| Eco24I | GRGCYC | 1 cut(s) | 567 |
| Eco31I | GGTCTC | 1 cut(s) | 467 |
| Eco47I | GGWCC | 5 cut(s) | 39, 256, 325, 448, 541 |
| Eco53kI | GAGCTC | 1 cut(s) | 565 |
| Eco81I | CCTNAGG | 1 cut(s) | 432 |
| EcoICRI | GAGCTC | 1 cut(s) | 565 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 548 |
| EcoO109I | RGGNCCY | 1 cut(s) | 448 |
| EcoRII | CCWGG | 2 cut(s) | 109, 444 |
| EcoT38I | GRGCYC | 1 cut(s) | 567 |
| FaeI | CATG | 3 cut(s) | 195, 212, 289 |
| FaiI | YATR | 5 cut(s) | 193, 210, 287, 521, 525 |
| FaqI | GGGAC | 1 cut(s) | 434 |
| FatI | CATG | 3 cut(s) | 191, 208, 285 |
| FbaI | TGATCA | 1 cut(s) | 124 |
| Fnu4HI | GCNGC | 4 cut(s) | 6, 75, 487, 516 |
| FokI | GGATG | 3 cut(s) | 164, 252, 330 |
| FriOI | GRGCYC | 1 cut(s) | 567 |
| Fsp4HI | GCNGC | 4 cut(s) | 6, 75, 487, 516 |
| FspBI | CTAG | 3 cut(s) | 363, 420, 482 |
| GlaI | GCGC | 2 cut(s) | 263, 578 |
| GluI | GCNGC | 4 cut(s) | 6, 75, 487, 516 |
| GsuI | CTGGAG | 1 cut(s) | 93 |
| HaeII | RGCGCY | 1 cut(s) | 265 |
| HaeIII | GGCC | 3 cut(s) | 207, 504, 611 |
| HapII | CCGG | 3 cut(s) | 84, 204, 254 |
| HhaI | GCGC | 2 cut(s) | 264, 579 |
| Hin1II | CATG | 3 cut(s) | 195, 212, 289 |
| Hin6I | GCGC | 2 cut(s) | 262, 577 |
| HinP1I | GCGC | 2 cut(s) | 262, 577 |
| HincII | GTYRAC | 3 cut(s) | 370, 388, 415 |
| HindII | GTYRAC | 3 cut(s) | 370, 388, 415 |
| HindIII | AAGCTT | 1 cut(s) | 219 |
| HinfI | GANTC | 2 cut(s) | 457, 627 |
| HpaI | GTTAAC | 1 cut(s) | 370 |
| HpaII | CCGG | 3 cut(s) | 84, 204, 254 |
| Hpy166II | GTNNAC | 4 cut(s) | 370, 388, 395, 415 |
| Hpy188I | TCNGA | 2 cut(s) | 39, 66 |
| Hpy188III | TCNNGA | 1 cut(s) | 624 |
| Hpy8I | GTNNAC | 4 cut(s) | 370, 388, 395, 415 |
| Hpy99I | CGWCG | 2 cut(s) | 23, 70 |
| HpyAV | CCTTC | 1 cut(s) | 17 |
| HpyCH4III | ACNGT | 3 cut(s) | 296, 379, 392 |
| HpyCH4V | TGCA | 2 cut(s) | 191, 395 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 492, 501 |
| HpyF3I | CTNAG | 1 cut(s) | 432 |
| Hsp92II | CATG | 3 cut(s) | 195, 212, 289 |
| HspAI | GCGC | 2 cut(s) | 262, 577 |
| Ksp22I | TGATCA | 1 cut(s) | 124 |
| KspAI | GTTAAC | 1 cut(s) | 370 |
| Kzo9I | GATC | 2 cut(s) | 10, 124 |
| LguI | GCTCTTC | 1 cut(s) | 309 |
| LmnI | GCTCC | 1 cut(s) | 562 |
| Lsp1109I | GCAGC | 2 cut(s) | 17, 498 |
| MaeI | CTAG | 3 cut(s) | 363, 420, 482 |
| MaeIII | GTNAC | 1 cut(s) | 436 |
| MalI | GATC | 2 cut(s) | 12, 126 |
| MboI | GATC | 2 cut(s) | 10, 124 |
| MboII | GAAGA | 1 cut(s) | 326 |
| MhlI | GDGCHC | 2 cut(s) | 397, 567 |
| MlsI | TGGCCA | 1 cut(s) | 611 |
| MluCI | AATT | 2 cut(s) | 50, 279 |
| MluNI | TGGCCA | 1 cut(s) | 611 |
| MmeI | TCCRAC | 3 cut(s) | 17, 89, 137 |
| MnlI | CCTC | 6 cut(s) | 8, 69, 72, 441, 521, 554 |
| Mox20I | TGGCCA | 1 cut(s) | 611 |
| MscI | TGGCCA | 1 cut(s) | 611 |
| MseI | TTAA | 4 cut(s) | 117, 173, 369, 381 |
| MslI | CAYNNNNRTG | 1 cut(s) | 617 |
| Msp20I | TGGCCA | 1 cut(s) | 611 |
| MspA1I | CMGCKG | 3 cut(s) | 8, 74, 470 |
| MspI | CCGG | 3 cut(s) | 84, 204, 254 |
| MspR9I | CCNGG | 2 cut(s) | 111, 446 |
| MvaI | CCWGG | 2 cut(s) | 111, 446 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 492, 501 |
| NdeII | GATC | 2 cut(s) | 10, 124 |
| NheI | GCTAGC | 1 cut(s) | 362 |
| NlaIII | CATG | 3 cut(s) | 195, 212, 289 |
| NlaIV | GGNNCC | 3 cut(s) | 41, 429, 450 |
| NmeAIII | GCCGAG | 1 cut(s) | 118 |
| PciSI | GCTCTTC | 1 cut(s) | 309 |
| PfeI | GAWTC | 2 cut(s) | 457, 627 |
| PinAI | ACCGGT | 1 cut(s) | 83 |
| PkrI | GCNGC | 4 cut(s) | 7, 76, 488, 517 |
| PpuMI | RGGWCCY | 1 cut(s) | 448 |
| Psp124BI | GAGCTC | 1 cut(s) | 567 |
| Psp5II | RGGWCCY | 1 cut(s) | 448 |
| Psp6I | CCWGG | 2 cut(s) | 109, 444 |
| PspGI | CCWGG | 2 cut(s) | 109, 444 |
| PspN4I | GGNNCC | 3 cut(s) | 41, 429, 450 |
| PspPI | GGNCC | 5 cut(s) | 39, 256, 325, 448, 541 |
| PspPPI | RGGWCCY | 1 cut(s) | 448 |
| RseI | CAYNNNNRTG | 1 cut(s) | 617 |
| SacI | GAGCTC | 1 cut(s) | 567 |
| SapI | GCTCTTC | 1 cut(s) | 309 |
| SaqAI | TTAA | 4 cut(s) | 117, 173, 369, 381 |
| SatI | GCNGC | 4 cut(s) | 6, 75, 487, 516 |
| Sau3AI | GATC | 2 cut(s) | 10, 124 |
| Sau96I | GGNCC | 5 cut(s) | 39, 256, 325, 448, 541 |
| ScrFI | CCNGG | 2 cut(s) | 111, 446 |
| SduI | GDGCHC | 2 cut(s) | 397, 567 |
| SinI | GGWCC | 5 cut(s) | 39, 256, 325, 448, 541 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 617 |
| Sse9I | AATT | 2 cut(s) | 50, 279 |
| SspMI | CTAG | 3 cut(s) | 363, 420, 482 |
| SstI | GAGCTC | 1 cut(s) | 567 |
| StyD4I | CCNGG | 2 cut(s) | 109, 444 |
| TaaI | ACNGT | 3 cut(s) | 296, 379, 392 |
| TaqI | TCGA | 3 cut(s) | 13, 567, 630 |
| TasI | AATT | 2 cut(s) | 50, 279 |
| TauI | GCSGC | 2 cut(s) | 77, 518 |
| TfiI | GAWTC | 2 cut(s) | 457, 627 |
| Tru1I | TTAA | 4 cut(s) | 117, 173, 369, 381 |
| Tru9I | TTAA | 4 cut(s) | 117, 173, 369, 381 |
| TscAI | CASTG | 1 cut(s) | 459 |
| TseI | GCWGC | 2 cut(s) | 5, 486 |
| TspRI | CASTG | 1 cut(s) | 459 |
| VneI | GTGCAC | 1 cut(s) | 393 |
| VpaK11BI | GGWCC | 5 cut(s) | 39, 256, 325, 448, 541 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 548 |
| XapI | RAATTY | 1 cut(s) | 50 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 355 |
| XspI | CTAG | 3 cut(s) | 363, 420, 482 |