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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 469, 769 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 253 |
| Acc36I | ACCTGC | 2 cut(s) | 469, 769 |
| Acc65I | GGTACC | 1 cut(s) | 29 |
| AccB1I | GGYRCC | 1 cut(s) | 29 |
| AccII | CGCG | 2 cut(s) | 131, 244 |
| AclWI | GGATC | 1 cut(s) | 784 |
| AcoI | YGGCCR | 3 cut(s) | 68, 285, 483 |
| AfaI | GTAC | 2 cut(s) | 31, 589 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 45, 248, 478, 590, 730 |
| AgsI | TTSAA | 1 cut(s) | 266 |
| AjnI | CCWGG | 2 cut(s) | 70, 569 |
| AleI | CACNNNNGTG | 1 cut(s) | 491 |
| AluBI | AGCT | 7 cut(s) | 11, 418, 464, 523, 649, 682, 845 |
| AluI | AGCT | 7 cut(s) | 11, 418, 464, 523, 649, 682, 845 |
| Alw21I | GWGCWC | 2 cut(s) | 331, 569 |
| Alw26I | GTCTC | 2 cut(s) | 356, 743 |
| AlwI | GGATC | 1 cut(s) | 784 |
| Ama87I | CYCGRG | 1 cut(s) | 370 |
| AoxI | GGCC | 7 cut(s) | 68, 215, 285, 391, 468, 483, 662 |
| ApeKI | GCWGC | 2 cut(s) | 126, 378 |
| AseI | ATTAAT | 1 cut(s) | 599 |
| Asp718I | GGTACC | 1 cut(s) | 29 |
| AspA2I | CCTAGG | 1 cut(s) | 352 |
| AspLEI | GCGC | 3 cut(s) | 131, 133, 596 |
| AspS9I | GGNCC | 5 cut(s) | 239, 246, 251, 469, 662 |
| AsuC2I | CCSGG | 6 cut(s) | 94, 308, 344, 371, 372, 542 |
| AsuHPI | GGTGA | 2 cut(s) | 394, 852 |
| AsuNHI | GCTAGC | 1 cut(s) | 841 |
| AvaI | CYCGRG | 1 cut(s) | 370 |
| AvaII | GGWCC | 3 cut(s) | 239, 246, 251 |
| AvrII | CCTAGG | 1 cut(s) | 352 |
| BaeGI | GKGCMC | 1 cut(s) | 678 |
| BalI | TGGCCA | 1 cut(s) | 485 |
| BanI | GGYRCC | 1 cut(s) | 29 |
| BanII | GRGCYC | 1 cut(s) | 93 |
| Bbv12I | GWGCWC | 2 cut(s) | 331, 569 |
| BbvCI | CCTCAGC | 2 cut(s) | 116, 465 |
| BbvI | GCAGC | 2 cut(s) | 113, 390 |
| BccI | CCATC | 2 cut(s) | 142, 831 |
| BceAI | ACGGC | 1 cut(s) | 83 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 264, 298 |
| BciT130I | CCWGG | 2 cut(s) | 72, 571 |
| BcnI | CCSGG | 6 cut(s) | 94, 308, 344, 371, 372, 542 |
| BcoDI | GTCTC | 2 cut(s) | 356, 743 |
| BfaI | CTAG | 6 cut(s) | 24, 353, 585, 735, 791, 842 |
| BfuAI | ACCTGC | 2 cut(s) | 469, 769 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 76, 390 |
| BglII | AGATCT | 1 cut(s) | 627 |
| BisI | GCNGC | 4 cut(s) | 127, 379, 382, 665 |
| BlnI | CCTAGG | 1 cut(s) | 352 |
| BlsI | GCNGC | 4 cut(s) | 128, 380, 383, 666 |
| Bme1390I | CCNGG | 8 cut(s) | 72, 94, 308, 344, 371, 372, 542, 571 |
| Bme18I | GGWCC | 3 cut(s) | 239, 246, 251 |
| BmeT110I | CYCGRG | 1 cut(s) | 370 |
| BmgT120I | GGNCC | 5 cut(s) | 239, 246, 251, 469, 662 |
| BmiI | GGNNCC | 2 cut(s) | 31, 663 |
| BmrFI | CCNGG | 8 cut(s) | 72, 94, 308, 344, 371, 372, 542, 571 |
| BmrI | ACTGGG | 1 cut(s) | 816 |
| BmsI | GCATC | 1 cut(s) | 156 |
| BmtI | GCTAGC | 1 cut(s) | 845 |
| BmuI | ACTGGG | 1 cut(s) | 816 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 27 |
| BpmI | CTGGAG | 1 cut(s) | 545 |
| Bpu10I | CCTNAGC | 2 cut(s) | 116, 465 |
| BpuEI | CTTGAG | 1 cut(s) | 748 |
| BpuMI | CCSGG | 6 cut(s) | 94, 308, 344, 371, 372, 542 |
| BsaJI | CCNNGG | 5 cut(s) | 92, 242, 342, 352, 370 |
| BsaWI | WCCGGW | 1 cut(s) | 248 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 45, 248, 478, 590, 730 |
| Bse118I | RCCGGY | 1 cut(s) | 590 |
| Bse1I | ACTGG | 3 cut(s) | 280, 611, 811 |
| BseBI | CCWGG | 2 cut(s) | 72, 571 |
| BseDI | CCNNGG | 5 cut(s) | 92, 242, 342, 352, 370 |
| BseGI | GGATG | 1 cut(s) | 134 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 45, 248, 478, 590, 730 |
| BseMII | CTCAG | 5 cut(s) | 107, 242, 456, 539, 781 |
| BseNI | ACTGG | 3 cut(s) | 280, 611, 811 |
| BsePI | GCGCGC | 1 cut(s) | 129 |
| BseSI | GKGCMC | 1 cut(s) | 678 |
| BseXI | GCAGC | 2 cut(s) | 113, 390 |
| BseYI | CCCAGC | 2 cut(s) | 165, 678 |
| BsgI | GTGCAG | 1 cut(s) | 698 |
| Bsh1236I | CGCG | 2 cut(s) | 131, 244 |
| BshFI | GGCC | 7 cut(s) | 70, 217, 287, 393, 470, 485, 664 |
| BshNI | GGYRCC | 1 cut(s) | 29 |
| BsiHKAI | GWGCWC | 2 cut(s) | 331, 569 |
| BsiHKCI | CYCGRG | 1 cut(s) | 370 |
| BsiSI | CCGG | 7 cut(s) | 94, 249, 308, 344, 371, 542, 591 |
| BslFI | GGGAC | 2 cut(s) | 171, 820 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 45, 248, 478, 590, 730 |
| BsmAI | GTCTC | 2 cut(s) | 356, 743 |
| BsmFI | GGGAC | 2 cut(s) | 171, 820 |
| BsnI | GGCC | 7 cut(s) | 70, 217, 287, 393, 470, 485, 664 |
| BsoBI | CYCGRG | 1 cut(s) | 370 |
| Bsp1286I | GDGCHC | 4 cut(s) | 93, 331, 569, 678 |
| Bsp143I | GATC | 7 cut(s) | 107, 155, 206, 269, 627, 776, 816 |
| BspANI | GGCC | 7 cut(s) | 70, 217, 287, 393, 470, 485, 664 |
| BspCNI | CTCAG | 5 cut(s) | 108, 241, 457, 540, 780 |
| BspFNI | CGCG | 2 cut(s) | 131, 244 |
| BspLI | GGNNCC | 2 cut(s) | 31, 663 |
| BspMI | ACCTGC | 2 cut(s) | 469, 769 |
| BspOI | GCTAGC | 1 cut(s) | 845 |
| BspPI | GGATC | 1 cut(s) | 784 |
| BspT107I | GGYRCC | 1 cut(s) | 29 |
| BsrFI | RCCGGY | 1 cut(s) | 590 |
| BsrI | ACTGG | 3 cut(s) | 280, 611, 811 |
| BssAI | RCCGGY | 1 cut(s) | 590 |
| BssECI | CCNNGG | 5 cut(s) | 92, 242, 342, 352, 370 |
| BssHII | GCGCGC | 1 cut(s) | 129 |
| BssMI | GATC | 7 cut(s) | 107, 155, 206, 269, 627, 776, 816 |
| BssT1I | CCWWGG | 1 cut(s) | 352 |
| Bst2UI | CCWGG | 2 cut(s) | 72, 571 |
| Bst4CI | ACNGT | 1 cut(s) | 255 |
| Bst6I | CTCTTC | 1 cut(s) | 807 |
| BstC8I | GCNNGC | 4 cut(s) | 77, 131, 420, 843 |
| BstDEI | CTNAG | 5 cut(s) | 116, 228, 465, 548, 767 |
| BstDSI | CCRYGG | 1 cut(s) | 242 |
| BstF5I | GGATG | 1 cut(s) | 134 |
| BstFNI | CGCG | 2 cut(s) | 131, 244 |
| BstHHI | GCGC | 3 cut(s) | 131, 133, 596 |
| BstKTI | GATC | 7 cut(s) | 110, 158, 209, 272, 630, 779, 819 |
| BstMAI | GTCTC | 2 cut(s) | 356, 743 |
| BstMBI | GATC | 7 cut(s) | 107, 155, 206, 269, 627, 776, 816 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 76, 390, 542, 631, 673 |
| BstNI | CCWGG | 2 cut(s) | 72, 571 |
| BstSCI | CCNGG | 8 cut(s) | 70, 92, 306, 342, 369, 370, 540, 569 |
| BstSLI | GKGCMC | 1 cut(s) | 678 |
| BstUI | CGCG | 2 cut(s) | 131, 244 |
| BstV1I | GCAGC | 2 cut(s) | 113, 390 |
| BstX2I | RGATCY | 1 cut(s) | 627 |
| BstYI | RGATCY | 1 cut(s) | 627 |
| BsuRI | GGCC | 7 cut(s) | 70, 217, 287, 393, 470, 485, 664 |
| BtgI | CCRYGG | 1 cut(s) | 242 |
| BtsCI | GGATG | 1 cut(s) | 134 |
| BtsI | GCAGTG | 3 cut(s) | 110, 121, 710 |
| BtsIMutI | CAGTG | 5 cut(s) | 110, 121, 545, 710, 804 |
| BveI | ACCTGC | 2 cut(s) | 469, 769 |
| Cac8I | GCNNGC | 4 cut(s) | 77, 131, 420, 843 |
| CfoI | GCGC | 3 cut(s) | 131, 133, 596 |
| Cfr10I | RCCGGY | 1 cut(s) | 590 |
| Cfr13I | GGNCC | 5 cut(s) | 239, 246, 251, 469, 662 |
| Cfr42I | CCGCGG | 1 cut(s) | 245 |
| Cfr9I | CCCGGG | 1 cut(s) | 370 |
| CpoI | CGGWCCG | 3 cut(s) | 239, 246, 251 |
| CseI | GACGC | 2 cut(s) | 171, 776 |
| Csp6I | GTAC | 2 cut(s) | 30, 588 |
| CspI | CGGWCCG | 3 cut(s) | 239, 246, 251 |
| CviQI | GTAC | 2 cut(s) | 30, 588 |
| DdeI | CTNAG | 5 cut(s) | 116, 228, 465, 548, 767 |
| DpnI | GATC | 7 cut(s) | 109, 157, 208, 271, 629, 778, 818 |
| DpnII | GATC | 7 cut(s) | 107, 155, 206, 269, 627, 776, 816 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 253 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 253 |
| EaeI | YGGCCR | 3 cut(s) | 68, 285, 483 |
| Eam1104I | CTCTTC | 1 cut(s) | 807 |
| EarI | CTCTTC | 1 cut(s) | 807 |
| Eco130I | CCWWGG | 1 cut(s) | 352 |
| Eco24I | GRGCYC | 1 cut(s) | 93 |
| Eco47I | GGWCC | 3 cut(s) | 239, 246, 251 |
| Eco88I | CYCGRG | 1 cut(s) | 370 |
| EcoRII | CCWGG | 2 cut(s) | 70, 569 |
| EcoT14I | CCWWGG | 1 cut(s) | 352 |
| EcoT38I | GRGCYC | 1 cut(s) | 93 |
| ErhI | CCWWGG | 1 cut(s) | 352 |
| FaiI | YATR | 5 cut(s) | 18, 412, 414, 621, 623 |
| FaqI | GGGAC | 2 cut(s) | 171, 820 |
| FauI | CCCGC | 2 cut(s) | 68, 846 |
| FauNDI | CATATG | 1 cut(s) | 621 |
| Fnu4HI | GCNGC | 4 cut(s) | 127, 379, 382, 665 |
| FokI | GGATG | 1 cut(s) | 121 |
| FriOI | GRGCYC | 1 cut(s) | 93 |
| Fsp4HI | GCNGC | 4 cut(s) | 127, 379, 382, 665 |
| FspBI | CTAG | 6 cut(s) | 24, 353, 585, 735, 791, 842 |
| GlaI | GCGC | 3 cut(s) | 130, 132, 595 |
| GluI | GCNGC | 4 cut(s) | 127, 379, 382, 665 |
| GsaI | CCCAGC | 2 cut(s) | 169, 682 |
| GsuI | CTGGAG | 1 cut(s) | 545 |
| HaeIII | GGCC | 7 cut(s) | 70, 217, 287, 393, 470, 485, 664 |
| HapII | CCGG | 7 cut(s) | 94, 249, 308, 344, 371, 542, 591 |
| HgaI | GACGC | 2 cut(s) | 171, 776 |
| HhaI | GCGC | 3 cut(s) | 131, 133, 596 |
| Hin6I | GCGC | 3 cut(s) | 129, 131, 594 |
| HinP1I | GCGC | 3 cut(s) | 129, 131, 594 |
| HinfI | GANTC | 2 cut(s) | 170, 704 |
| HpaII | CCGG | 7 cut(s) | 94, 249, 308, 344, 371, 542, 591 |
| HphI | GGTGA | 2 cut(s) | 394, 852 |
| Hpy166II | GTNNAC | 2 cut(s) | 349, 443 |
| Hpy188I | TCNGA | 2 cut(s) | 231, 831 |
| Hpy188III | TCNNGA | 3 cut(s) | 159, 210, 791 |
| Hpy8I | GTNNAC | 2 cut(s) | 349, 443 |
| Hpy99I | CGWCG | 1 cut(s) | 259 |
| HpyAV | CCTTC | 2 cut(s) | 207, 873 |
| HpyCH4III | ACNGT | 1 cut(s) | 255 |
| HpyCH4IV | ACGT | 4 cut(s) | 439, 488, 499, 798 |
| HpyCH4V | TGCA | 3 cut(s) | 619, 625, 715 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 76, 390, 542, 631, 673 |
| HpyF3I | CTNAG | 5 cut(s) | 116, 228, 465, 548, 767 |
| HpySE526I | ACGT | 4 cut(s) | 439, 488, 499, 798 |
| HspAI | GCGC | 3 cut(s) | 129, 131, 594 |
| KpnI | GGTACC | 1 cut(s) | 33 |
| KspI | CCGCGG | 1 cut(s) | 245 |
| Kzo9I | GATC | 7 cut(s) | 107, 155, 206, 269, 627, 776, 816 |
| LmnI | GCTCC | 2 cut(s) | 564, 691 |
| Lsp1109I | GCAGC | 2 cut(s) | 113, 390 |
| LweI | GCATC | 1 cut(s) | 156 |
| MaeI | CTAG | 6 cut(s) | 24, 353, 585, 735, 791, 842 |
| MaeII | ACGT | 4 cut(s) | 439, 488, 499, 798 |
| MaeIII | GTNAC | 3 cut(s) | 50, 495, 799 |
| MalI | GATC | 7 cut(s) | 109, 157, 208, 271, 629, 778, 818 |
| MboI | GATC | 7 cut(s) | 107, 155, 206, 269, 627, 776, 816 |
| MboII | GAAGA | 2 cut(s) | 216, 824 |
| MflI | RGATCY | 1 cut(s) | 627 |
| MhlI | GDGCHC | 4 cut(s) | 93, 331, 569, 678 |
| MlsI | TGGCCA | 1 cut(s) | 485 |
| MluCI | AATT | 1 cut(s) | 261 |
| MluNI | TGGCCA | 1 cut(s) | 485 |
| MmeI | TCCRAC | 1 cut(s) | 669 |
| Mox20I | TGGCCA | 1 cut(s) | 485 |
| MscI | TGGCCA | 1 cut(s) | 485 |
| MseI | TTAA | 2 cut(s) | 260, 599 |
| MslI | CAYNNNNRTG | 1 cut(s) | 491 |
| Msp20I | TGGCCA | 1 cut(s) | 485 |
| MspA1I | CMGCKG | 1 cut(s) | 244 |
| MspI | CCGG | 7 cut(s) | 94, 249, 308, 344, 371, 542, 591 |
| MspR9I | CCNGG | 8 cut(s) | 72, 94, 308, 344, 371, 372, 542, 571 |
| MvaI | CCWGG | 2 cut(s) | 72, 571 |
| MvnI | CGCG | 2 cut(s) | 131, 244 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 76, 390, 542, 631, 673 |
| NciI | CCSGG | 6 cut(s) | 94, 308, 344, 371, 372, 542 |
| NdeI | CATATG | 1 cut(s) | 621 |
| NdeII | GATC | 7 cut(s) | 107, 155, 206, 269, 627, 776, 816 |
| NheI | GCTAGC | 1 cut(s) | 841 |
| NlaIV | GGNNCC | 2 cut(s) | 31, 663 |
| NmuCI | GTSAC | 2 cut(s) | 50, 495 |
| OliI | CACNNNNGTG | 1 cut(s) | 491 |
| PaqCI | CACCTGC | 2 cut(s) | 469, 769 |
| PauI | GCGCGC | 1 cut(s) | 129 |
| PfeI | GAWTC | 2 cut(s) | 170, 704 |
| PkrI | GCNGC | 4 cut(s) | 128, 380, 383, 666 |
| PshBI | ATTAAT | 1 cut(s) | 599 |
| Psp6I | CCWGG | 2 cut(s) | 70, 569 |
| PspFI | CCCAGC | 2 cut(s) | 165, 678 |
| PspGI | CCWGG | 2 cut(s) | 70, 569 |
| PspN4I | GGNNCC | 2 cut(s) | 31, 663 |
| PspPI | GGNCC | 5 cut(s) | 239, 246, 251, 469, 662 |
| PsuI | RGATCY | 1 cut(s) | 627 |
| PteI | GCGCGC | 1 cut(s) | 129 |
| RsaI | GTAC | 2 cut(s) | 31, 589 |
| RsaNI | GTAC | 2 cut(s) | 30, 588 |
| RseI | CAYNNNNRTG | 1 cut(s) | 491 |
| Rsr2I | CGGWCCG | 3 cut(s) | 239, 246, 251 |
| RsrII | CGGWCCG | 3 cut(s) | 239, 246, 251 |
| SacII | CCGCGG | 1 cut(s) | 245 |
| SaqAI | TTAA | 2 cut(s) | 260, 599 |
| SatI | GCNGC | 4 cut(s) | 127, 379, 382, 665 |
| Sau3AI | GATC | 7 cut(s) | 107, 155, 206, 269, 627, 776, 816 |
| Sau96I | GGNCC | 5 cut(s) | 239, 246, 251, 469, 662 |
| ScrFI | CCNGG | 8 cut(s) | 72, 94, 308, 344, 371, 372, 542, 571 |
| SduI | GDGCHC | 4 cut(s) | 93, 331, 569, 678 |
| SfaNI | GCATC | 1 cut(s) | 156 |
| Sfr303I | CCGCGG | 1 cut(s) | 245 |
| SgrBI | CCGCGG | 1 cut(s) | 245 |
| SinI | GGWCC | 3 cut(s) | 239, 246, 251 |
| SmaI | CCCGGG | 1 cut(s) | 372 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 491 |
| SmlI | CTYRAG | 1 cut(s) | 727 |
| SmoI | CTYRAG | 1 cut(s) | 727 |
| Sse9I | AATT | 1 cut(s) | 261 |
| SspMI | CTAG | 6 cut(s) | 24, 353, 585, 735, 791, 842 |
| StyD4I | CCNGG | 8 cut(s) | 70, 92, 306, 342, 369, 370, 540, 569 |
| StyI | CCWWGG | 1 cut(s) | 352 |
| TaaI | ACNGT | 1 cut(s) | 255 |
| TaiI | ACGT | 4 cut(s) | 442, 491, 502, 801 |
| TaqI | TCGA | 2 cut(s) | 173, 194 |
| TasI | AATT | 1 cut(s) | 261 |
| TauI | GCSGC | 2 cut(s) | 384, 667 |
| TfiI | GAWTC | 2 cut(s) | 170, 704 |
| Tru1I | TTAA | 2 cut(s) | 260, 599 |
| Tru9I | TTAA | 2 cut(s) | 260, 599 |
| TscAI | CASTG | 5 cut(s) | 117, 128, 552, 717, 811 |
| TseFI | GTSAC | 2 cut(s) | 50, 495 |
| TseI | GCWGC | 2 cut(s) | 126, 378 |
| Tsp45I | GTSAC | 2 cut(s) | 50, 495 |
| TspDTI | ATGAA | 2 cut(s) | 522, 797 |
| TspGWI | ACGGA | 1 cut(s) | 577 |
| TspMI | CCCGGG | 1 cut(s) | 370 |
| TspRI | CASTG | 5 cut(s) | 117, 128, 552, 717, 811 |
| VpaK11BI | GGWCC | 3 cut(s) | 239, 246, 251 |
| VspI | ATTAAT | 1 cut(s) | 599 |
| XbaI | TCTAGA | 1 cut(s) | 790 |
| XmaI | CCCGGG | 1 cut(s) | 370 |
| XmaJI | CCTAGG | 1 cut(s) | 352 |
| XspI | CTAG | 6 cut(s) | 24, 353, 585, 735, 791, 842 |