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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 65, 235 |
| AciI | CCGC | 8 cut(s) | 6, 39, 63, 79, 136, 182, 215, 235 |
| AclWI | GGATC | 3 cut(s) | 159, 192, 245 |
| AcoI | YGGCCR | 1 cut(s) | 99 |
| AcuI | CTGAAG | 1 cut(s) | 39 |
| AfaI | GTAC | 1 cut(s) | 167 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 68, 300 |
| AjnI | CCWGG | 3 cut(s) | 124, 142, 147 |
| AlwI | GGATC | 3 cut(s) | 159, 192, 245 |
| AoxI | GGCC | 3 cut(s) | 99, 145, 284 |
| ApeKI | GCWGC | 3 cut(s) | 129, 132, 307 |
| AspLEI | GCGC | 2 cut(s) | 235, 312 |
| AsuC2I | CCSGG | 2 cut(s) | 98, 108 |
| AsuHPI | GGTGA | 1 cut(s) | 151 |
| BbvI | GCAGC | 3 cut(s) | 141, 144, 294 |
| BccI | CCATC | 2 cut(s) | 50, 166 |
| BciT130I | CCWGG | 3 cut(s) | 126, 144, 149 |
| BcnI | CCSGG | 2 cut(s) | 98, 108 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 144 |
| BisI | GCNGC | 4 cut(s) | 130, 133, 136, 308 |
| BlsI | GCNGC | 4 cut(s) | 131, 134, 137, 309 |
| Bme1390I | CCNGG | 5 cut(s) | 98, 108, 126, 144, 149 |
| BmrFI | CCNGG | 5 cut(s) | 98, 108, 126, 144, 149 |
| BpuMI | CCSGG | 2 cut(s) | 98, 108 |
| BsaJI | CCNNGG | 2 cut(s) | 96, 106 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 68, 300 |
| Bse1I | ACTGG | 1 cut(s) | 183 |
| BseBI | CCWGG | 3 cut(s) | 126, 144, 149 |
| BseDI | CCNNGG | 2 cut(s) | 96, 106 |
| BseGI | GGATG | 1 cut(s) | 42 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 68, 300 |
| BseNI | ACTGG | 1 cut(s) | 183 |
| BseRI | GAGGAG | 2 cut(s) | 280, 283 |
| BseXI | GCAGC | 3 cut(s) | 141, 144, 294 |
| Bsh1236I | CGCG | 2 cut(s) | 65, 235 |
| BshFI | GGCC | 3 cut(s) | 101, 147, 286 |
| BsiSI | CCGG | 2 cut(s) | 98, 108 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 68, 300 |
| BsnI | GGCC | 3 cut(s) | 101, 147, 286 |
| Bsp143I | GATC | 3 cut(s) | 151, 184, 237 |
| BspACI | CCGC | 8 cut(s) | 6, 39, 63, 79, 136, 182, 215, 235 |
| BspANI | GGCC | 3 cut(s) | 101, 147, 286 |
| BspFNI | CGCG | 2 cut(s) | 65, 235 |
| BspHI | TCATGA | 1 cut(s) | 314 |
| BspPI | GGATC | 3 cut(s) | 159, 192, 245 |
| BspQI | GCTCTTC | 1 cut(s) | 15 |
| BsrI | ACTGG | 1 cut(s) | 183 |
| BssECI | CCNNGG | 2 cut(s) | 96, 106 |
| BssMI | GATC | 3 cut(s) | 151, 184, 237 |
| Bst2UI | CCWGG | 3 cut(s) | 126, 144, 149 |
| Bst6I | CTCTTC | 1 cut(s) | 15 |
| BstF5I | GGATG | 1 cut(s) | 42 |
| BstFNI | CGCG | 2 cut(s) | 65, 235 |
| BstHHI | GCGC | 2 cut(s) | 235, 312 |
| BstKTI | GATC | 3 cut(s) | 154, 187, 240 |
| BstMBI | GATC | 3 cut(s) | 151, 184, 237 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 78, 135, 144 |
| BstNI | CCWGG | 3 cut(s) | 126, 144, 149 |
| BstSCI | CCNGG | 5 cut(s) | 96, 106, 124, 142, 147 |
| BstUI | CGCG | 2 cut(s) | 65, 235 |
| BstV1I | GCAGC | 3 cut(s) | 141, 144, 294 |
| BstX2I | RGATCY | 3 cut(s) | 151, 184, 237 |
| BstYI | RGATCY | 3 cut(s) | 151, 184, 237 |
| BsuRI | GGCC | 3 cut(s) | 101, 147, 286 |
| BtsCI | GGATG | 1 cut(s) | 42 |
| BtsIMutI | CAGTG | 1 cut(s) | 176 |
| CciI | TCATGA | 1 cut(s) | 314 |
| CfoI | GCGC | 2 cut(s) | 235, 312 |
| Csp6I | GTAC | 1 cut(s) | 166 |
| CviAII | CATG | 2 cut(s) | 163, 315 |
| CviJI | RGCY | 6 cut(s) | 101, 135, 147, 286, 292, 307 |
| CviKI_1 | RGCY | 6 cut(s) | 101, 135, 147, 286, 292, 307 |
| CviQI | GTAC | 1 cut(s) | 166 |
| DpnI | GATC | 3 cut(s) | 153, 186, 239 |
| DpnII | GATC | 3 cut(s) | 151, 184, 237 |
| EaeI | YGGCCR | 1 cut(s) | 99 |
| Eam1104I | CTCTTC | 1 cut(s) | 15 |
| EarI | CTCTTC | 1 cut(s) | 15 |
| EciI | GGCGGA | 2 cut(s) | 197, 230 |
| Eco57I | CTGAAG | 1 cut(s) | 39 |
| EcoRII | CCWGG | 3 cut(s) | 124, 142, 147 |
| FaeI | CATG | 2 cut(s) | 166, 318 |
| FaiI | YATR | 4 cut(s) | 164, 192, 245, 316 |
| FatI | CATG | 2 cut(s) | 162, 314 |
| FauI | CCCGC | 2 cut(s) | 46, 70 |
| Fnu4HI | GCNGC | 4 cut(s) | 130, 133, 136, 308 |
| FokI | GGATG | 1 cut(s) | 29 |
| Fsp4HI | GCNGC | 4 cut(s) | 130, 133, 136, 308 |
| GlaI | GCGC | 2 cut(s) | 234, 311 |
| GluI | GCNGC | 4 cut(s) | 130, 133, 136, 308 |
| HaeIII | GGCC | 3 cut(s) | 101, 147, 286 |
| HapII | CCGG | 2 cut(s) | 98, 108 |
| HhaI | GCGC | 2 cut(s) | 235, 312 |
| Hin1II | CATG | 2 cut(s) | 166, 318 |
| Hin6I | GCGC | 2 cut(s) | 233, 310 |
| HinP1I | GCGC | 2 cut(s) | 233, 310 |
| HincII | GTYRAC | 1 cut(s) | 76 |
| HindII | GTYRAC | 1 cut(s) | 76 |
| HpaI | GTTAAC | 1 cut(s) | 76 |
| HpaII | CCGG | 2 cut(s) | 98, 108 |
| HphI | GGTGA | 1 cut(s) | 151 |
| Hpy166II | GTNNAC | 1 cut(s) | 76 |
| Hpy188I | TCNGA | 1 cut(s) | 58 |
| Hpy188III | TCNNGA | 2 cut(s) | 91, 315 |
| Hpy8I | GTNNAC | 1 cut(s) | 76 |
| HpyAV | CCTTC | 2 cut(s) | 223, 276 |
| HpyCH4IV | ACGT | 1 cut(s) | 208 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 78, 135, 144 |
| HpySE526I | ACGT | 1 cut(s) | 208 |
| Hsp92II | CATG | 2 cut(s) | 166, 318 |
| HspAI | GCGC | 2 cut(s) | 233, 310 |
| KspAI | GTTAAC | 1 cut(s) | 76 |
| Kzo9I | GATC | 3 cut(s) | 151, 184, 237 |
| LguI | GCTCTTC | 1 cut(s) | 15 |
| LmnI | GCTCC | 2 cut(s) | 258, 270 |
| Lsp1109I | GCAGC | 3 cut(s) | 141, 144, 294 |
| MaeII | ACGT | 1 cut(s) | 208 |
| MaeIII | GTNAC | 1 cut(s) | 157 |
| MalI | GATC | 3 cut(s) | 153, 186, 239 |
| MboI | GATC | 3 cut(s) | 151, 184, 237 |
| MboII | GAAGA | 2 cut(s) | 32, 232 |
| MflI | RGATCY | 3 cut(s) | 151, 184, 237 |
| MmeI | TCCRAC | 1 cut(s) | 81 |
| MnlI | CCTC | 5 cut(s) | 64, 109, 205, 258, 261 |
| MseI | TTAA | 1 cut(s) | 75 |
| MspA1I | CMGCKG | 1 cut(s) | 6 |
| MspI | CCGG | 2 cut(s) | 98, 108 |
| MspR9I | CCNGG | 5 cut(s) | 98, 108, 126, 144, 149 |
| MvaI | CCWGG | 3 cut(s) | 126, 144, 149 |
| MvnI | CGCG | 2 cut(s) | 65, 235 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 78, 135, 144 |
| NciI | CCSGG | 2 cut(s) | 98, 108 |
| NdeII | GATC | 3 cut(s) | 151, 184, 237 |
| NlaIII | CATG | 2 cut(s) | 166, 318 |
| NmuCI | GTSAC | 1 cut(s) | 157 |
| PagI | TCATGA | 1 cut(s) | 314 |
| PciSI | GCTCTTC | 1 cut(s) | 15 |
| PkrI | GCNGC | 4 cut(s) | 131, 134, 137, 309 |
| Psp6I | CCWGG | 3 cut(s) | 124, 142, 147 |
| PspGI | CCWGG | 3 cut(s) | 124, 142, 147 |
| PsuI | RGATCY | 3 cut(s) | 151, 184, 237 |
| RsaI | GTAC | 1 cut(s) | 167 |
| RsaNI | GTAC | 1 cut(s) | 166 |
| SapI | GCTCTTC | 1 cut(s) | 15 |
| SaqAI | TTAA | 1 cut(s) | 75 |
| SatI | GCNGC | 4 cut(s) | 130, 133, 136, 308 |
| Sau3AI | GATC | 3 cut(s) | 151, 184, 237 |
| ScrFI | CCNGG | 5 cut(s) | 98, 108, 126, 144, 149 |
| SetI | ASST | 2 cut(s) | 211, 305 |
| SsiI | CCGC | 8 cut(s) | 6, 39, 63, 79, 136, 182, 215, 235 |
| StyD4I | CCNGG | 5 cut(s) | 96, 106, 124, 142, 147 |
| TaiI | ACGT | 1 cut(s) | 211 |
| TauI | GCSGC | 1 cut(s) | 138 |
| Tru1I | TTAA | 1 cut(s) | 75 |
| Tru9I | TTAA | 1 cut(s) | 75 |
| TscAI | CASTG | 1 cut(s) | 183 |
| TseFI | GTSAC | 1 cut(s) | 157 |
| TseI | GCWGC | 3 cut(s) | 129, 132, 307 |
| Tsp45I | GTSAC | 1 cut(s) | 157 |
| TspDTI | ATGAA | 1 cut(s) | 232 |
| TspRI | CASTG | 1 cut(s) | 183 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 169 |