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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 115 |
| Acc65I | GGTACC | 1 cut(s) | 451 |
| AccB1I | GGYRCC | 1 cut(s) | 451 |
| AccI | GTMKAC | 1 cut(s) | 430 |
| AciI | CCGC | 3 cut(s) | 40, 263, 456 |
| AclI | AACGTT | 1 cut(s) | 384 |
| AclWI | GGATC | 1 cut(s) | 181 |
| AcoI | YGGCCR | 1 cut(s) | 333 |
| AcuI | CTGAAG | 1 cut(s) | 383 |
| AfaI | GTAC | 2 cut(s) | 418, 453 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 103, 186, 274, 440 |
| AjnI | CCWGG | 4 cut(s) | 179, 223, 228, 294 |
| AluBI | AGCT | 2 cut(s) | 34, 361 |
| AluI | AGCT | 2 cut(s) | 34, 361 |
| Alw21I | GWGCWC | 1 cut(s) | 59 |
| Alw26I | GTCTC | 1 cut(s) | 101 |
| Alw44I | GTGCAC | 1 cut(s) | 55 |
| AlwI | GGATC | 1 cut(s) | 181 |
| Ama87I | CYCGRG | 1 cut(s) | 201 |
| AoxI | GGCC | 5 cut(s) | 177, 226, 312, 318, 333 |
| ApaLI | GTGCAC | 1 cut(s) | 55 |
| ApeKI | GCWGC | 2 cut(s) | 34, 47 |
| Asp718I | GGTACC | 1 cut(s) | 451 |
| AspLEI | GCGC | 3 cut(s) | 52, 303, 373 |
| AspS9I | GGNCC | 4 cut(s) | 183, 232, 265, 319 |
| AsuC2I | CCSGG | 2 cut(s) | 167, 187 |
| AvaI | CYCGRG | 1 cut(s) | 201 |
| AvaII | GGWCC | 3 cut(s) | 183, 232, 265 |
| BaeGI | GKGCMC | 1 cut(s) | 59 |
| BalI | TGGCCA | 1 cut(s) | 335 |
| BanI | GGYRCC | 1 cut(s) | 451 |
| Bbv12I | GWGCWC | 1 cut(s) | 59 |
| BbvI | GCAGC | 2 cut(s) | 21, 34 |
| BceAI | ACGGC | 2 cut(s) | 37, 149 |
| BciT130I | CCWGG | 4 cut(s) | 181, 225, 230, 296 |
| BcnI | CCSGG | 2 cut(s) | 167, 187 |
| BcoDI | GTCTC | 1 cut(s) | 101 |
| BfaI | CTAG | 2 cut(s) | 137, 420 |
| BfoI | RGCGCY | 1 cut(s) | 374 |
| BfuAI | ACCTGC | 1 cut(s) | 115 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 225 |
| BisI | GCNGC | 2 cut(s) | 35, 48 |
| BlsI | GCNGC | 2 cut(s) | 36, 49 |
| BmcAI | AGTACT | 1 cut(s) | 418 |
| Bme1390I | CCNGG | 6 cut(s) | 167, 181, 187, 225, 230, 296 |
| Bme18I | GGWCC | 3 cut(s) | 183, 232, 265 |
| BmeT110I | CYCGRG | 1 cut(s) | 201 |
| BmgT120I | GGNCC | 4 cut(s) | 183, 232, 265, 319 |
| BmiI | GGNNCC | 1 cut(s) | 453 |
| BmrFI | CCNGG | 6 cut(s) | 167, 181, 187, 225, 230, 296 |
| BmsI | GCATC | 1 cut(s) | 71 |
| BpuMI | CCSGG | 2 cut(s) | 167, 187 |
| BsaJI | CCNNGG | 1 cut(s) | 315 |
| BsaWI | WCCGGW | 1 cut(s) | 10 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 103, 186, 274, 440 |
| Bse1I | ACTGG | 1 cut(s) | 445 |
| BseBI | CCWGG | 4 cut(s) | 181, 225, 230, 296 |
| BseDI | CCNNGG | 1 cut(s) | 315 |
| BseGI | GGATG | 1 cut(s) | 86 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 103, 186, 274, 440 |
| BseNI | ACTGG | 1 cut(s) | 445 |
| BseRI | GAGGAG | 1 cut(s) | 119 |
| BseSI | GKGCMC | 1 cut(s) | 59 |
| BseXI | GCAGC | 2 cut(s) | 21, 34 |
| BsgI | GTGCAG | 2 cut(s) | 20, 143 |
| Bsh1285I | CGRYCG | 1 cut(s) | 15 |
| BshFI | GGCC | 5 cut(s) | 179, 228, 314, 320, 335 |
| BshNI | GGYRCC | 1 cut(s) | 451 |
| BsiEI | CGRYCG | 1 cut(s) | 15 |
| BsiHKAI | GWGCWC | 1 cut(s) | 59 |
| BsiHKCI | CYCGRG | 1 cut(s) | 201 |
| BsiSI | CCGG | 5 cut(s) | 11, 147, 157, 166, 186 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 103, 186, 274, 440 |
| BsmAI | GTCTC | 1 cut(s) | 101 |
| BsmBI | CGTCTC | 1 cut(s) | 101 |
| BsnI | GGCC | 5 cut(s) | 179, 228, 314, 320, 335 |
| BsoBI | CYCGRG | 1 cut(s) | 201 |
| Bsp1286I | GDGCHC | 1 cut(s) | 59 |
| Bsp143I | GATC | 2 cut(s) | 151, 173 |
| BspACI | CCGC | 3 cut(s) | 40, 263, 456 |
| BspANI | GGCC | 5 cut(s) | 179, 228, 314, 320, 335 |
| BspLI | GGNNCC | 1 cut(s) | 453 |
| BspMI | ACCTGC | 1 cut(s) | 115 |
| BspPI | GGATC | 1 cut(s) | 181 |
| BspT107I | GGYRCC | 1 cut(s) | 451 |
| BsrI | ACTGG | 1 cut(s) | 445 |
| BssECI | CCNNGG | 1 cut(s) | 315 |
| BssMI | GATC | 2 cut(s) | 151, 173 |
| BssT1I | CCWWGG | 1 cut(s) | 315 |
| Bst2UI | CCWGG | 4 cut(s) | 181, 225, 230, 296 |
| Bst4CI | ACNGT | 1 cut(s) | 61 |
| BstC8I | GCNNGC | 2 cut(s) | 208, 404 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 272 |
| BstF5I | GGATG | 1 cut(s) | 86 |
| BstH2I | RGCGCY | 1 cut(s) | 374 |
| BstHHI | GCGC | 3 cut(s) | 52, 303, 373 |
| BstKTI | GATC | 2 cut(s) | 154, 176 |
| BstMAI | GTCTC | 1 cut(s) | 101 |
| BstMBI | GATC | 2 cut(s) | 151, 173 |
| BstMCI | CGRYCG | 1 cut(s) | 15 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 162, 216, 225, 403 |
| BstNI | CCWGG | 4 cut(s) | 181, 225, 230, 296 |
| BstSCI | CCNGG | 6 cut(s) | 165, 179, 185, 223, 228, 294 |
| BstSLI | GKGCMC | 1 cut(s) | 59 |
| BstV1I | GCAGC | 2 cut(s) | 21, 34 |
| BsuRI | GGCC | 5 cut(s) | 179, 228, 314, 320, 335 |
| BtsCI | GGATG | 1 cut(s) | 86 |
| BtsIMutI | CAGTG | 1 cut(s) | 66 |
| BveI | ACCTGC | 1 cut(s) | 115 |
| Cac8I | GCNNGC | 2 cut(s) | 208, 404 |
| CfoI | GCGC | 3 cut(s) | 52, 303, 373 |
| Cfr13I | GGNCC | 4 cut(s) | 183, 232, 265, 319 |
| Csp6I | GTAC | 2 cut(s) | 417, 452 |
| CviAII | CATG | 2 cut(s) | 244, 337 |
| CviQI | GTAC | 2 cut(s) | 417, 452 |
| DpnI | GATC | 2 cut(s) | 153, 175 |
| DpnII | GATC | 2 cut(s) | 151, 173 |
| EaeI | YGGCCR | 1 cut(s) | 333 |
| EciI | GGCGGA | 1 cut(s) | 278 |
| Eco130I | CCWWGG | 1 cut(s) | 315 |
| Eco47I | GGWCC | 3 cut(s) | 183, 232, 265 |
| Eco57I | CTGAAG | 1 cut(s) | 383 |
| Eco88I | CYCGRG | 1 cut(s) | 201 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 272 |
| EcoO109I | RGGNCCY | 1 cut(s) | 232 |
| EcoRII | CCWGG | 4 cut(s) | 179, 223, 228, 294 |
| EcoT14I | CCWWGG | 1 cut(s) | 315 |
| ErhI | CCWWGG | 1 cut(s) | 315 |
| Esp3I | CGTCTC | 1 cut(s) | 101 |
| FaeI | CATG | 2 cut(s) | 247, 340 |
| FaiI | YATR | 5 cut(s) | 245, 249, 273, 338, 467 |
| FatI | CATG | 2 cut(s) | 243, 336 |
| FauI | CCCGC | 1 cut(s) | 463 |
| FblI | GTMKAC | 1 cut(s) | 430 |
| Fnu4HI | GCNGC | 2 cut(s) | 35, 48 |
| FokI | GGATG | 1 cut(s) | 93 |
| Fsp4HI | GCNGC | 2 cut(s) | 35, 48 |
| FspBI | CTAG | 2 cut(s) | 137, 420 |
| GlaI | GCGC | 3 cut(s) | 51, 302, 372 |
| GluI | GCNGC | 2 cut(s) | 35, 48 |
| HaeII | RGCGCY | 1 cut(s) | 374 |
| HaeIII | GGCC | 5 cut(s) | 179, 228, 314, 320, 335 |
| HapII | CCGG | 5 cut(s) | 11, 147, 157, 166, 186 |
| HhaI | GCGC | 3 cut(s) | 52, 303, 373 |
| Hin1II | CATG | 2 cut(s) | 247, 340 |
| Hin6I | GCGC | 3 cut(s) | 50, 301, 371 |
| HinP1I | GCGC | 3 cut(s) | 50, 301, 371 |
| HincII | GTYRAC | 1 cut(s) | 388 |
| HindII | GTYRAC | 1 cut(s) | 388 |
| HinfI | GANTC | 3 cut(s) | 17, 112, 351 |
| HpaII | CCGG | 5 cut(s) | 11, 147, 157, 166, 186 |
| Hpy166II | GTNNAC | 4 cut(s) | 57, 382, 388, 431 |
| Hpy188I | TCNGA | 2 cut(s) | 22, 173 |
| Hpy188III | TCNNGA | 1 cut(s) | 348 |
| Hpy8I | GTNNAC | 4 cut(s) | 57, 382, 388, 431 |
| Hpy99I | CGWCG | 1 cut(s) | 359 |
| HpyAV | CCTTC | 2 cut(s) | 94, 361 |
| HpyCH4III | ACNGT | 1 cut(s) | 61 |
| HpyCH4IV | ACGT | 1 cut(s) | 384 |
| HpyCH4V | TGCA | 4 cut(s) | 37, 57, 124, 397 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 162, 216, 225, 403 |
| HpySE526I | ACGT | 1 cut(s) | 384 |
| Hsp92II | CATG | 2 cut(s) | 247, 340 |
| HspAI | GCGC | 3 cut(s) | 50, 301, 371 |
| KpnI | GGTACC | 1 cut(s) | 455 |
| Kzo9I | GATC | 2 cut(s) | 151, 173 |
| LmnI | GCTCC | 2 cut(s) | 286, 298 |
| Lsp1109I | GCAGC | 2 cut(s) | 21, 34 |
| LweI | GCATC | 1 cut(s) | 71 |
| MaeI | CTAG | 2 cut(s) | 137, 420 |
| MaeII | ACGT | 1 cut(s) | 384 |
| MaeIII | GTNAC | 1 cut(s) | 290 |
| MalI | GATC | 2 cut(s) | 153, 175 |
| MboI | GATC | 2 cut(s) | 151, 173 |
| MboII | GAAGA | 2 cut(s) | 376, 456 |
| MhlI | GDGCHC | 1 cut(s) | 59 |
| MlsI | TGGCCA | 1 cut(s) | 335 |
| MluNI | TGGCCA | 1 cut(s) | 335 |
| MlyI | GAGTC | 2 cut(s) | 26, 121 |
| MmeI | TCCRAC | 1 cut(s) | 151 |
| MnlI | CCTC | 5 cut(s) | 71, 97, 133, 190, 278 |
| Mox20I | TGGCCA | 1 cut(s) | 335 |
| MscI | TGGCCA | 1 cut(s) | 335 |
| MslI | CAYNNNNRTG | 2 cut(s) | 341, 470 |
| Msp20I | TGGCCA | 1 cut(s) | 335 |
| MspI | CCGG | 5 cut(s) | 11, 147, 157, 166, 186 |
| MspR9I | CCNGG | 6 cut(s) | 167, 181, 187, 225, 230, 296 |
| MvaI | CCWGG | 4 cut(s) | 181, 225, 230, 296 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 162, 216, 225, 403 |
| NciI | CCSGG | 2 cut(s) | 167, 187 |
| NdeII | GATC | 2 cut(s) | 151, 173 |
| NlaIII | CATG | 2 cut(s) | 247, 340 |
| NlaIV | GGNNCC | 1 cut(s) | 453 |
| NmuCI | GTSAC | 1 cut(s) | 290 |
| PaeR7I | CTCGAG | 1 cut(s) | 201 |
| PfeI | GAWTC | 1 cut(s) | 351 |
| PkrI | GCNGC | 2 cut(s) | 36, 49 |
| PleI | GAGTC | 2 cut(s) | 25, 120 |
| PpsI | GAGTC | 2 cut(s) | 25, 120 |
| PpuMI | RGGWCCY | 1 cut(s) | 232 |
| Psp1406I | AACGTT | 1 cut(s) | 384 |
| Psp5II | RGGWCCY | 1 cut(s) | 232 |
| Psp6I | CCWGG | 4 cut(s) | 179, 223, 228, 294 |
| PspGI | CCWGG | 4 cut(s) | 179, 223, 228, 294 |
| PspN4I | GGNNCC | 1 cut(s) | 453 |
| PspPI | GGNCC | 4 cut(s) | 183, 232, 265, 319 |
| PspPPI | RGGWCCY | 1 cut(s) | 232 |
| PspXI | VCTCGAGB | 1 cut(s) | 201 |
| RsaI | GTAC | 2 cut(s) | 418, 453 |
| RsaNI | GTAC | 2 cut(s) | 417, 452 |
| RseI | CAYNNNNRTG | 2 cut(s) | 341, 470 |
| SatI | GCNGC | 2 cut(s) | 35, 48 |
| Sau3AI | GATC | 2 cut(s) | 151, 173 |
| Sau96I | GGNCC | 4 cut(s) | 183, 232, 265, 319 |
| ScaI | AGTACT | 1 cut(s) | 418 |
| SchI | GAGTC | 2 cut(s) | 26, 121 |
| ScrFI | CCNGG | 6 cut(s) | 167, 181, 187, 225, 230, 296 |
| SduI | GDGCHC | 1 cut(s) | 59 |
| SetI | ASST | 7 cut(s) | 36, 129, 144, 237, 270, 363, 387 |
| SfaNI | GCATC | 1 cut(s) | 71 |
| Sfr274I | CTCGAG | 1 cut(s) | 201 |
| SinI | GGWCC | 3 cut(s) | 183, 232, 265 |
| SlaI | CTCGAG | 1 cut(s) | 201 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 341, 470 |
| SmlI | CTYRAG | 1 cut(s) | 201 |
| SmoI | CTYRAG | 1 cut(s) | 201 |
| SsiI | CCGC | 3 cut(s) | 40, 263, 456 |
| SspMI | CTAG | 2 cut(s) | 137, 420 |
| StyD4I | CCNGG | 6 cut(s) | 165, 179, 185, 223, 228, 294 |
| StyI | CCWWGG | 1 cut(s) | 315 |
| TaaI | ACNGT | 1 cut(s) | 61 |
| TaiI | ACGT | 1 cut(s) | 387 |
| TaqI | TCGA | 3 cut(s) | 15, 202, 354 |
| TatI | WGTACW | 1 cut(s) | 416 |
| TfiI | GAWTC | 1 cut(s) | 351 |
| TscAI | CASTG | 1 cut(s) | 66 |
| TseFI | GTSAC | 1 cut(s) | 290 |
| TseI | GCWGC | 2 cut(s) | 34, 47 |
| Tsp45I | GTSAC | 1 cut(s) | 290 |
| TspRI | CASTG | 1 cut(s) | 66 |
| VneI | GTGCAC | 1 cut(s) | 55 |
| VpaK11BI | GGWCC | 3 cut(s) | 183, 232, 265 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 272 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 250 |
| XhoI | CTCGAG | 1 cut(s) | 201 |
| XmiI | GTMKAC | 1 cut(s) | 430 |
| XspI | CTAG | 2 cut(s) | 137, 420 |
| ZrmI | AGTACT | 1 cut(s) | 418 |