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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 155 |
| AgsI | TTSAA | 2 cut(s) | 20, 121 |
| AluBI | AGCT | 1 cut(s) | 52 |
| AluI | AGCT | 1 cut(s) | 52 |
| ApoI | RAATTY | 1 cut(s) | 155 |
| BccI | CCATC | 1 cut(s) | 21 |
| CviJI | RGCY | 2 cut(s) | 52, 67 |
| CviKI_1 | RGCY | 2 cut(s) | 52, 67 |
| DraI | TTTAAA | 1 cut(s) | 154 |
| FaiI | YATR | 3 cut(s) | 55, 135, 137 |
| HinfI | GANTC | 2 cut(s) | 74, 94 |
| Hpy188I | TCNGA | 2 cut(s) | 64, 148 |
| Hpy188III | TCNNGA | 1 cut(s) | 78 |
| Hpy99I | CGWCG | 1 cut(s) | 43 |
| HpyCH4IV | ACGT | 1 cut(s) | 38 |
| HpySE526I | ACGT | 1 cut(s) | 38 |
| LpnPI | CCDG | 2 cut(s) | 62, 81 |
| MaeII | ACGT | 1 cut(s) | 38 |
| MboII | GAAGA | 1 cut(s) | 138 |
| MluCI | AATT | 1 cut(s) | 155 |
| MlyI | GAGTC | 2 cut(s) | 83, 103 |
| MseI | TTAA | 1 cut(s) | 153 |
| PleI | GAGTC | 2 cut(s) | 82, 102 |
| PpsI | GAGTC | 2 cut(s) | 82, 102 |
| SaqAI | TTAA | 1 cut(s) | 153 |
| SchI | GAGTC | 2 cut(s) | 83, 103 |
| SetI | ASST | 2 cut(s) | 41, 54 |
| SgeI | CNNG | 8 cut(s) | 32, 61, 76, 80, 90, 105, 110, 127 |
| Sse9I | AATT | 1 cut(s) | 155 |
| TaiI | ACGT | 1 cut(s) | 41 |
| TaqI | TCGA | 2 cut(s) | 92, 108 |
| TasI | AATT | 1 cut(s) | 155 |
| Tru1I | TTAA | 1 cut(s) | 153 |
| Tru9I | TTAA | 1 cut(s) | 153 |
| TspDTI | ATGAA | 1 cut(s) | 93 |
| XapI | RAATTY | 1 cut(s) | 155 |