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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 334 |
| Acc36I | ACCTGC | 1 cut(s) | 25 |
| AciI | CCGC | 3 cut(s) | 15, 167, 231 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AclWI | GGATC | 1 cut(s) | 231 |
| AcsI | RAATTY | 1 cut(s) | 341 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AjnI | CCWGG | 4 cut(s) | 133, 147, 159, 196 |
| AluBI | AGCT | 1 cut(s) | 244 |
| AluI | AGCT | 1 cut(s) | 244 |
| AlwI | GGATC | 1 cut(s) | 231 |
| AoxI | GGCC | 2 cut(s) | 189, 309 |
| ApeKI | GCWGC | 1 cut(s) | 201 |
| ApoI | RAATTY | 1 cut(s) | 341 |
| AspLEI | GCGC | 2 cut(s) | 294, 364 |
| AspS9I | GGNCC | 3 cut(s) | 163, 190, 310 |
| AsuHPI | GGTGA | 1 cut(s) | 220 |
| AvaII | GGWCC | 1 cut(s) | 163 |
| BaeGI | GKGCMC | 2 cut(s) | 112, 211 |
| BanII | GRGCYC | 1 cut(s) | 155 |
| BauI | CACGAG | 1 cut(s) | 71 |
| BbvCI | CCTCAGC | 1 cut(s) | 306 |
| BbvI | GCAGC | 1 cut(s) | 213 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 4 cut(s) | 135, 149, 161, 198 |
| BfoI | RGCGCY | 1 cut(s) | 365 |
| BfuAI | ACCTGC | 1 cut(s) | 25 |
| BisI | GCNGC | 1 cut(s) | 202 |
| BlsI | GCNGC | 1 cut(s) | 203 |
| Bme1390I | CCNGG | 4 cut(s) | 135, 149, 161, 198 |
| Bme18I | GGWCC | 1 cut(s) | 163 |
| BmgT120I | GGNCC | 3 cut(s) | 163, 190, 310 |
| BmiI | GGNNCC | 2 cut(s) | 144, 165 |
| BmrFI | CCNGG | 4 cut(s) | 135, 149, 161, 198 |
| BpmI | CTGGAG | 1 cut(s) | 386 |
| Bpu10I | CCTNAGC | 1 cut(s) | 306 |
| BpuEI | CTTGAG | 1 cut(s) | 12 |
| BsaJI | CCNNGG | 4 cut(s) | 133, 147, 148, 159 |
| BsaWI | WCCGGW | 1 cut(s) | 124 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BseBI | CCWGG | 4 cut(s) | 135, 149, 161, 198 |
| BseDI | CCNNGG | 4 cut(s) | 133, 147, 148, 159 |
| BseGI | GGATG | 2 cut(s) | 58, 155 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BseMII | CTCAG | 1 cut(s) | 297 |
| BseRI | GAGGAG | 1 cut(s) | 200 |
| BseSI | GKGCMC | 2 cut(s) | 112, 211 |
| BseXI | GCAGC | 1 cut(s) | 213 |
| BshFI | GGCC | 2 cut(s) | 191, 311 |
| BsiSI | CCGG | 1 cut(s) | 125 |
| BslFI | GGGAC | 1 cut(s) | 149 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BsmFI | GGGAC | 1 cut(s) | 149 |
| BsnI | GGCC | 2 cut(s) | 191, 311 |
| Bsp1286I | GDGCHC | 3 cut(s) | 112, 155, 211 |
| Bsp143I | GATC | 2 cut(s) | 40, 223 |
| BspACI | CCGC | 3 cut(s) | 15, 167, 231 |
| BspANI | GGCC | 2 cut(s) | 191, 311 |
| BspCNI | CTCAG | 1 cut(s) | 298 |
| BspLI | GGNNCC | 2 cut(s) | 144, 165 |
| BspMI | ACCTGC | 1 cut(s) | 25 |
| BspPI | GGATC | 1 cut(s) | 231 |
| BssECI | CCNNGG | 4 cut(s) | 133, 147, 148, 159 |
| BssMI | GATC | 2 cut(s) | 40, 223 |
| BssSI | CACGAG | 1 cut(s) | 71 |
| Bst2BI | CACGAG | 1 cut(s) | 71 |
| Bst2UI | CCWGG | 4 cut(s) | 135, 149, 161, 198 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 94 |
| BstC8I | GCNNGC | 2 cut(s) | 246, 284 |
| BstDEI | CTNAG | 1 cut(s) | 306 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 2 cut(s) | 58, 155 |
| BstH2I | RGCGCY | 1 cut(s) | 365 |
| BstHHI | GCGC | 2 cut(s) | 294, 364 |
| BstKTI | GATC | 2 cut(s) | 43, 226 |
| BstMBI | GATC | 2 cut(s) | 40, 223 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 368 |
| BstNI | CCWGG | 4 cut(s) | 135, 149, 161, 198 |
| BstSCI | CCNGG | 4 cut(s) | 133, 147, 159, 196 |
| BstSLI | GKGCMC | 2 cut(s) | 112, 211 |
| BstV1I | GCAGC | 1 cut(s) | 213 |
| BstX2I | RGATCY | 1 cut(s) | 223 |
| BstYI | RGATCY | 1 cut(s) | 223 |
| BsuRI | GGCC | 2 cut(s) | 191, 311 |
| BtsCI | GGATG | 2 cut(s) | 58, 155 |
| BtsI | GCAGTG | 1 cut(s) | 211 |
| BtsIMutI | CAGTG | 1 cut(s) | 211 |
| BveI | ACCTGC | 1 cut(s) | 25 |
| Cac8I | GCNNGC | 2 cut(s) | 246, 284 |
| CfoI | GCGC | 2 cut(s) | 294, 364 |
| Cfr13I | GGNCC | 3 cut(s) | 163, 190, 310 |
| CseI | GACGC | 1 cut(s) | 337 |
| CviJI | RGCY | 7 cut(s) | 77, 153, 191, 244, 254, 305, 311 |
| CviKI_1 | RGCY | 7 cut(s) | 77, 153, 191, 244, 254, 305, 311 |
| DdeI | CTNAG | 1 cut(s) | 306 |
| DpnI | GATC | 2 cut(s) | 42, 225 |
| DpnII | GATC | 2 cut(s) | 40, 223 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 334 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 334 |
| Eco24I | GRGCYC | 1 cut(s) | 155 |
| Eco47I | GGWCC | 1 cut(s) | 163 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoO109I | RGGNCCY | 1 cut(s) | 163 |
| EcoRII | CCWGG | 4 cut(s) | 133, 147, 159, 196 |
| EcoT38I | GRGCYC | 1 cut(s) | 155 |
| FaiI | YATR | 3 cut(s) | 236, 240, 388 |
| FaqI | GGGAC | 1 cut(s) | 149 |
| FauI | CCCGC | 2 cut(s) | 8, 174 |
| Fnu4HI | GCNGC | 1 cut(s) | 202 |
| FokI | GGATG | 2 cut(s) | 45, 142 |
| FriOI | GRGCYC | 1 cut(s) | 155 |
| Fsp4HI | GCNGC | 1 cut(s) | 202 |
| GlaI | GCGC | 2 cut(s) | 293, 363 |
| GluI | GCNGC | 1 cut(s) | 202 |
| GsuI | CTGGAG | 1 cut(s) | 386 |
| HaeII | RGCGCY | 1 cut(s) | 365 |
| HaeIII | GGCC | 2 cut(s) | 191, 311 |
| HapII | CCGG | 1 cut(s) | 125 |
| HgaI | GACGC | 1 cut(s) | 337 |
| HhaI | GCGC | 2 cut(s) | 294, 364 |
| Hin6I | GCGC | 2 cut(s) | 292, 362 |
| HinP1I | GCGC | 2 cut(s) | 292, 362 |
| HincII | GTYRAC | 3 cut(s) | 85, 97, 130 |
| HindII | GTYRAC | 3 cut(s) | 85, 97, 130 |
| HpaI | GTTAAC | 2 cut(s) | 85, 97 |
| HpaII | CCGG | 1 cut(s) | 125 |
| HphI | GGTGA | 1 cut(s) | 220 |
| Hpy166II | GTNNAC | 3 cut(s) | 85, 97, 130 |
| Hpy188III | TCNNGA | 2 cut(s) | 29, 221 |
| Hpy8I | GTNNAC | 3 cut(s) | 85, 97, 130 |
| Hpy99I | CGWCG | 3 cut(s) | 94, 331, 350 |
| HpyAV | CCTTC | 1 cut(s) | 295 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 94 |
| HpyCH4IV | ACGT | 1 cut(s) | 81 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 368 |
| HpyF3I | CTNAG | 1 cut(s) | 306 |
| HpySE526I | ACGT | 1 cut(s) | 81 |
| HspAI | GCGC | 2 cut(s) | 292, 362 |
| KspAI | GTTAAC | 2 cut(s) | 85, 97 |
| Kzo9I | GATC | 2 cut(s) | 40, 223 |
| Lsp1109I | GCAGC | 1 cut(s) | 213 |
| MaeII | ACGT | 1 cut(s) | 81 |
| MalI | GATC | 2 cut(s) | 42, 225 |
| MboI | GATC | 2 cut(s) | 40, 223 |
| MflI | RGATCY | 1 cut(s) | 223 |
| MhlI | GDGCHC | 3 cut(s) | 112, 155, 211 |
| MluCI | AATT | 2 cut(s) | 216, 341 |
| MnlI | CCTC | 7 cut(s) | 59, 67, 178, 181, 269, 301, 361 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| MslI | CAYNNNNRTG | 1 cut(s) | 233 |
| MspA1I | CMGCKG | 1 cut(s) | 169 |
| MspI | CCGG | 1 cut(s) | 125 |
| MspR9I | CCNGG | 4 cut(s) | 135, 149, 161, 198 |
| MvaI | CCWGG | 4 cut(s) | 135, 149, 161, 198 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 368 |
| NdeII | GATC | 2 cut(s) | 40, 223 |
| NlaIV | GGNNCC | 2 cut(s) | 144, 165 |
| PasI | CCCWGGG | 1 cut(s) | 148 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 78 |
| PkrI | GCNGC | 1 cut(s) | 203 |
| PpuMI | RGGWCCY | 1 cut(s) | 163 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| Psp5II | RGGWCCY | 1 cut(s) | 163 |
| Psp6I | CCWGG | 4 cut(s) | 133, 147, 159, 196 |
| PspGI | CCWGG | 4 cut(s) | 133, 147, 159, 196 |
| PspN4I | GGNNCC | 2 cut(s) | 144, 165 |
| PspPI | GGNCC | 3 cut(s) | 163, 190, 310 |
| PspPPI | RGGWCCY | 1 cut(s) | 163 |
| PsuI | RGATCY | 1 cut(s) | 223 |
| RseI | CAYNNNNRTG | 1 cut(s) | 233 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 1 cut(s) | 202 |
| Sau3AI | GATC | 2 cut(s) | 40, 223 |
| Sau96I | GGNCC | 3 cut(s) | 163, 190, 310 |
| ScrFI | CCNGG | 4 cut(s) | 135, 149, 161, 198 |
| SduI | GDGCHC | 3 cut(s) | 112, 155, 211 |
| SetI | ASST | 6 cut(s) | 39, 84, 165, 199, 246, 261 |
| SinI | GGWCC | 1 cut(s) | 163 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 233 |
| SmlI | CTYRAG | 1 cut(s) | 27 |
| SmoI | CTYRAG | 1 cut(s) | 27 |
| Sse9I | AATT | 2 cut(s) | 216, 341 |
| SsiI | CCGC | 3 cut(s) | 15, 167, 231 |
| StyD4I | CCNGG | 4 cut(s) | 133, 147, 159, 196 |
| TaaI | ACNGT | 2 cut(s) | 11, 94 |
| TaiI | ACGT | 1 cut(s) | 84 |
| TaqI | TCGA | 1 cut(s) | 345 |
| TasI | AATT | 2 cut(s) | 216, 341 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TscAI | CASTG | 1 cut(s) | 211 |
| TseI | GCWGC | 1 cut(s) | 201 |
| TspRI | CASTG | 1 cut(s) | 211 |
| VpaK11BI | GGWCC | 1 cut(s) | 163 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XapI | RAATTY | 1 cut(s) | 341 |