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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 187 |
| Acc36I | ACCTGC | 1 cut(s) | 187 |
| AciI | CCGC | 4 cut(s) | 38, 74, 89, 108 |
| AclWI | GGATC | 2 cut(s) | 48, 108 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 142, 196 |
| AluBI | AGCT | 1 cut(s) | 121 |
| AluI | AGCT | 1 cut(s) | 121 |
| Alw21I | GWGCWC | 1 cut(s) | 159 |
| AlwI | GGATC | 2 cut(s) | 48, 108 |
| AoxI | GGCC | 2 cut(s) | 180, 186 |
| AspS9I | GGNCC | 2 cut(s) | 133, 187 |
| AsuC2I | CCSGG | 1 cut(s) | 93 |
| AvaII | GGWCC | 1 cut(s) | 133 |
| Bbv12I | GWGCWC | 1 cut(s) | 159 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BccI | CCATC | 1 cut(s) | 66 |
| BcnI | CCSGG | 1 cut(s) | 93 |
| BfuAI | ACCTGC | 1 cut(s) | 187 |
| BisI | GCNGC | 1 cut(s) | 108 |
| BlsI | GCNGC | 1 cut(s) | 109 |
| Bme1390I | CCNGG | 1 cut(s) | 93 |
| Bme18I | GGWCC | 1 cut(s) | 133 |
| BmgT120I | GGNCC | 2 cut(s) | 133, 187 |
| BmrFI | CCNGG | 1 cut(s) | 93 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 1 cut(s) | 93 |
| BsaJI | CCNNGG | 1 cut(s) | 183 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 142, 196 |
| BseDI | CCNNGG | 1 cut(s) | 183 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 142, 196 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseYI | CCCAGC | 1 cut(s) | 70 |
| BshFI | GGCC | 2 cut(s) | 182, 188 |
| BsiHKAI | GWGCWC | 1 cut(s) | 159 |
| BsiSI | CCGG | 1 cut(s) | 92 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 142, 196 |
| BsnI | GGCC | 2 cut(s) | 182, 188 |
| Bsp1286I | GDGCHC | 1 cut(s) | 159 |
| Bsp143I | GATC | 2 cut(s) | 40, 100 |
| BspACI | CCGC | 4 cut(s) | 38, 74, 89, 108 |
| BspANI | GGCC | 2 cut(s) | 182, 188 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspMI | ACCTGC | 1 cut(s) | 187 |
| BspPI | GGATC | 2 cut(s) | 48, 108 |
| BssECI | CCNNGG | 1 cut(s) | 183 |
| BssMI | GATC | 2 cut(s) | 40, 100 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstC8I | GCNNGC | 3 cut(s) | 76, 89, 123 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 140 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstKTI | GATC | 2 cut(s) | 43, 103 |
| BstMBI | GATC | 2 cut(s) | 40, 100 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 84 |
| BstSCI | CCNGG | 1 cut(s) | 91 |
| BstX2I | RGATCY | 1 cut(s) | 100 |
| BstYI | RGATCY | 1 cut(s) | 100 |
| BsuRI | GGCC | 2 cut(s) | 182, 188 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BveI | ACCTGC | 1 cut(s) | 187 |
| Cac8I | GCNNGC | 3 cut(s) | 76, 89, 123 |
| Cfr13I | GGNCC | 2 cut(s) | 133, 187 |
| CviJI | RGCY | 3 cut(s) | 121, 182, 188 |
| CviKI_1 | RGCY | 3 cut(s) | 121, 182, 188 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 2 cut(s) | 42, 102 |
| DpnII | GATC | 2 cut(s) | 40, 100 |
| Eco47I | GGWCC | 1 cut(s) | 133 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 140 |
| FaiI | YATR | 2 cut(s) | 113, 117 |
| FauI | CCCGC | 2 cut(s) | 67, 96 |
| Fnu4HI | GCNGC | 1 cut(s) | 108 |
| FokI | GGATG | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 1 cut(s) | 108 |
| GluI | GCNGC | 1 cut(s) | 108 |
| GsaI | CCCAGC | 1 cut(s) | 74 |
| HaeIII | GGCC | 2 cut(s) | 182, 188 |
| HapII | CCGG | 1 cut(s) | 92 |
| HpaII | CCGG | 1 cut(s) | 92 |
| Hpy166II | GTNNAC | 2 cut(s) | 133, 202 |
| Hpy188III | TCNNGA | 1 cut(s) | 98 |
| Hpy8I | GTNNAC | 2 cut(s) | 133, 202 |
| HpyAV | CCTTC | 1 cut(s) | 172 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 84 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| Kzo9I | GATC | 2 cut(s) | 40, 100 |
| LmnI | GCTCC | 1 cut(s) | 154 |
| LpnPI | CCDG | 5 cut(s) | 83, 84, 105, 107, 182 |
| MalI | GATC | 2 cut(s) | 42, 102 |
| MboI | GATC | 2 cut(s) | 40, 100 |
| MflI | RGATCY | 1 cut(s) | 100 |
| MhlI | GDGCHC | 1 cut(s) | 159 |
| MnlI | CCTC | 4 cut(s) | 8, 22, 146, 178 |
| MslI | CAYNNNNRTG | 1 cut(s) | 209 |
| MspA1I | CMGCKG | 1 cut(s) | 74 |
| MspI | CCGG | 1 cut(s) | 92 |
| MspR9I | CCNGG | 1 cut(s) | 93 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 84 |
| NciI | CCSGG | 1 cut(s) | 93 |
| NdeII | GATC | 2 cut(s) | 40, 100 |
| NmeAIII | GCCGAG | 1 cut(s) | 208 |
| PaqCI | CACCTGC | 1 cut(s) | 187 |
| PkrI | GCNGC | 1 cut(s) | 109 |
| PspFI | CCCAGC | 1 cut(s) | 70 |
| PspPI | GGNCC | 2 cut(s) | 133, 187 |
| PsuI | RGATCY | 1 cut(s) | 100 |
| RseI | CAYNNNNRTG | 1 cut(s) | 209 |
| SatI | GCNGC | 1 cut(s) | 108 |
| Sau3AI | GATC | 2 cut(s) | 40, 100 |
| Sau96I | GGNCC | 2 cut(s) | 133, 187 |
| ScrFI | CCNGG | 1 cut(s) | 93 |
| SduI | GDGCHC | 1 cut(s) | 159 |
| SetI | ASST | 3 cut(s) | 123, 138, 201 |
| SinI | GGWCC | 1 cut(s) | 133 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 209 |
| SsiI | CCGC | 4 cut(s) | 38, 74, 89, 108 |
| StyD4I | CCNGG | 1 cut(s) | 91 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TauI | GCSGC | 1 cut(s) | 110 |
| VpaK11BI | GGWCC | 1 cut(s) | 133 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 140 |