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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 208 |
| AatII | GACGTC | 1 cut(s) | 1187 |
| Acc36I | ACCTGC | 2 cut(s) | 835, 1072 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 889, 1126 |
| AccBSI | CCGCTC | 2 cut(s) | 783, 1020 |
| AccI | GTMKAC | 1 cut(s) | 637 |
| AccII | CGCG | 2 cut(s) | 353, 619 |
| AclWI | GGATC | 9 cut(s) | 138, 404, 447, 480, 806, 1043, 1153, 1244, 1298 |
| AcoI | YGGCCR | 3 cut(s) | 387, 540, 585 |
| AcsI | RAATTY | 1 cut(s) | 53 |
| AcuI | CTGAAG | 3 cut(s) | 99, 218, 327 |
| AcyI | GRCGYC | 3 cut(s) | 360, 939, 1184 |
| AfaI | GTAC | 4 cut(s) | 455, 687, 790, 822 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 356, 535, 647, 798, 825, 889, 1035, 1062, 1126 |
| AflIII | ACRYGT | 1 cut(s) | 554 |
| AgsI | TTSAA | 3 cut(s) | 59, 172, 188 |
| AjnI | CCWGG | 7 cut(s) | 412, 435, 661, 700, 791, 924, 1028 |
| AluBI | AGCT | 3 cut(s) | 40, 886, 1123 |
| AluI | AGCT | 3 cut(s) | 40, 886, 1123 |
| Alw21I | GWGCWC | 2 cut(s) | 18, 42 |
| Alw26I | GTCTC | 2 cut(s) | 926, 1163 |
| AlwI | GGATC | 9 cut(s) | 138, 404, 447, 480, 806, 1043, 1153, 1244, 1298 |
| Ama87I | CYCGRG | 1 cut(s) | 784 |
| ApeKI | GCWGC | 3 cut(s) | 417, 420, 621 |
| ApoI | RAATTY | 1 cut(s) | 53 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 765 |
| AspA2I | CCTAGG | 2 cut(s) | 824, 1061 |
| AspLEI | GCGC | 7 cut(s) | 259, 510, 621, 626, 647, 831, 1068 |
| AspS9I | GGNCC | 4 cut(s) | 665, 719, 983, 1220 |
| AsuC2I | CCSGG | 7 cut(s) | 386, 396, 599, 981, 1163, 1218, 1234 |
| AsuHPI | GGTGA | 4 cut(s) | 413, 439, 821, 1058 |
| AvaI | CYCGRG | 1 cut(s) | 784 |
| AvaII | GGWCC | 1 cut(s) | 719 |
| AvrII | CCTAGG | 2 cut(s) | 824, 1061 |
| BaeGI | GKGCMC | 2 cut(s) | 140, 373 |
| BanII | GRGCYC | 1 cut(s) | 42 |
| BbsI | GAAGAC | 1 cut(s) | 206 |
| Bbv12I | GWGCWC | 2 cut(s) | 18, 42 |
| BbvI | GCAGC | 3 cut(s) | 429, 432, 633 |
| BccI | CCATC | 1 cut(s) | 338 |
| BceAI | ACGGC | 2 cut(s) | 234, 1232 |
| BciT130I | CCWGG | 7 cut(s) | 414, 437, 663, 702, 793, 926, 1030 |
| BcnI | CCSGG | 7 cut(s) | 386, 396, 599, 981, 1163, 1218, 1234 |
| BcoDI | GTCTC | 2 cut(s) | 926, 1163 |
| BfaI | CTAG | 3 cut(s) | 176, 825, 1062 |
| BfoI | RGCGCY | 2 cut(s) | 260, 648 |
| BfuAI | ACCTGC | 2 cut(s) | 835, 1072 |
| BglI | GCCNNNNNGGC | 4 cut(s) | 251, 432, 841, 1078 |
| BisI | GCNGC | 7 cut(s) | 255, 418, 421, 424, 543, 585, 622 |
| BlnI | CCTAGG | 2 cut(s) | 824, 1061 |
| BlsI | GCNGC | 7 cut(s) | 256, 419, 422, 425, 544, 586, 623 |
| Bme18I | GGWCC | 1 cut(s) | 719 |
| BmeT110I | CYCGRG | 1 cut(s) | 784 |
| BmgT120I | GGNCC | 4 cut(s) | 665, 719, 983, 1220 |
| BmiI | GGNNCC | 6 cut(s) | 85, 720, 959, 985, 1196, 1222 |
| BmsI | GCATC | 6 cut(s) | 648, 862, 888, 1099, 1125, 1185 |
| BoxI | GACNNNNGTC | 1 cut(s) | 938 |
| BpiI | GAAGAC | 1 cut(s) | 206 |
| BpmI | CTGGAG | 2 cut(s) | 929, 1166 |
| BpuMI | CCSGG | 7 cut(s) | 386, 396, 599, 981, 1163, 1218, 1234 |
| BsaAI | YACGTR | 1 cut(s) | 453 |
| BsaHI | GRCGYC | 3 cut(s) | 360, 939, 1184 |
| BsaJI | CCNNGG | 7 cut(s) | 394, 662, 701, 792, 824, 1029, 1061 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 119, 149, 902, 932, 1139, 1169 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 356, 535, 647, 798, 825, 889, 1035, 1062, 1126 |
| Bse118I | RCCGGY | 3 cut(s) | 647, 667, 1103 |
| Bse1I | ACTGG | 3 cut(s) | 471, 912, 1149 |
| BseBI | CCWGG | 7 cut(s) | 414, 437, 663, 702, 793, 926, 1030 |
| BseDI | CCNNGG | 7 cut(s) | 394, 662, 701, 792, 824, 1029, 1061 |
| BseGI | GGATG | 4 cut(s) | 330, 903, 1140, 1176 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 356, 535, 647, 798, 825, 889, 1035, 1062, 1126 |
| BseMII | CTCAG | 3 cut(s) | 44, 795, 1032 |
| BseNI | ACTGG | 3 cut(s) | 471, 912, 1149 |
| BseRI | GAGGAG | 2 cut(s) | 149, 152 |
| BseSI | GKGCMC | 2 cut(s) | 140, 373 |
| BseXI | GCAGC | 3 cut(s) | 429, 432, 633 |
| BseYI | CCCAGC | 4 cut(s) | 264, 641, 882, 1119 |
| Bsh1236I | CGCG | 2 cut(s) | 353, 619 |
| Bsh1285I | CGRYCG | 2 cut(s) | 357, 595 |
| BsiEI | CGRYCG | 2 cut(s) | 357, 595 |
| BsiHKAI | GWGCWC | 2 cut(s) | 18, 42 |
| BsiHKCI | CYCGRG | 1 cut(s) | 784 |
| BsiWI | CGTACG | 1 cut(s) | 453 |
| BslFI | GGGAC | 2 cut(s) | 732, 926 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 356, 535, 647, 798, 825, 889, 1035, 1062, 1126 |
| BsmAI | GTCTC | 2 cut(s) | 926, 1163 |
| BsmBI | CGTCTC | 2 cut(s) | 926, 1163 |
| BsmFI | GGGAC | 2 cut(s) | 732, 926 |
| BsmI | GAATGC | 1 cut(s) | 1030 |
| BsoBI | CYCGRG | 1 cut(s) | 784 |
| Bsp1286I | GDGCHC | 4 cut(s) | 18, 42, 140, 373 |
| BspCNI | CTCAG | 3 cut(s) | 43, 796, 1033 |
| BspFNI | CGCG | 2 cut(s) | 353, 619 |
| BspHI | TCATGA | 1 cut(s) | 549 |
| BspLI | GGNNCC | 6 cut(s) | 85, 720, 959, 985, 1196, 1222 |
| BspMI | ACCTGC | 2 cut(s) | 835, 1072 |
| BspPI | GGATC | 9 cut(s) | 138, 404, 447, 480, 806, 1043, 1153, 1244, 1298 |
| BspQI | GCTCTTC | 1 cut(s) | 303 |
| BsrBI | CCGCTC | 2 cut(s) | 783, 1020 |
| BsrFI | RCCGGY | 3 cut(s) | 647, 667, 1103 |
| BsrI | ACTGG | 3 cut(s) | 471, 912, 1149 |
| BssAI | RCCGGY | 3 cut(s) | 647, 667, 1103 |
| BssECI | CCNNGG | 7 cut(s) | 394, 662, 701, 792, 824, 1029, 1061 |
| BssNI | GRCGYC | 3 cut(s) | 360, 939, 1184 |
| BssT1I | CCWWGG | 2 cut(s) | 824, 1061 |
| Bst2UI | CCWGG | 7 cut(s) | 414, 437, 663, 702, 793, 926, 1030 |
| Bst4CI | ACNGT | 2 cut(s) | 75, 208 |
| Bst6I | CTCTTC | 3 cut(s) | 118, 183, 303 |
| BstACI | GRCGYC | 3 cut(s) | 360, 939, 1184 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 872 |
| BstBAI | YACGTR | 1 cut(s) | 453 |
| BstC8I | GCNNGC | 7 cut(s) | 611, 649, 833, 868, 1070, 1105, 1245 |
| BstDEI | CTNAG | 4 cut(s) | 30, 94, 804, 1041 |
| BstF5I | GGATG | 4 cut(s) | 330, 903, 1140, 1176 |
| BstFNI | CGCG | 2 cut(s) | 353, 619 |
| BstH2I | RGCGCY | 2 cut(s) | 260, 648 |
| BstHHI | GCGC | 7 cut(s) | 259, 510, 621, 626, 647, 831, 1068 |
| BstMAI | GTCTC | 2 cut(s) | 926, 1163 |
| BstMCI | CGRYCG | 2 cut(s) | 357, 595 |
| BstNI | CCWGG | 7 cut(s) | 414, 437, 663, 702, 793, 926, 1030 |
| BstNSI | RCATGY | 1 cut(s) | 686 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 938 |
| BstSLI | GKGCMC | 2 cut(s) | 140, 373 |
| BstUI | CGCG | 2 cut(s) | 353, 619 |
| BstV1I | GCAGC | 3 cut(s) | 429, 432, 633 |
| BstV2I | GAAGAC | 1 cut(s) | 206 |
| BstX2I | RGATCY | 5 cut(s) | 143, 409, 439, 472, 1236 |
| BstYI | RGATCY | 5 cut(s) | 143, 409, 439, 472, 1236 |
| BtgZI | GCGATG | 2 cut(s) | 885, 1122 |
| BtsCI | GGATG | 4 cut(s) | 330, 903, 1140, 1176 |
| BtsIMutI | CAGTG | 1 cut(s) | 464 |
| BveI | ACCTGC | 2 cut(s) | 835, 1072 |
| Cac8I | GCNNGC | 7 cut(s) | 611, 649, 833, 868, 1070, 1105, 1245 |
| CciI | TCATGA | 1 cut(s) | 549 |
| CfoI | GCGC | 7 cut(s) | 259, 510, 621, 626, 647, 831, 1068 |
| Cfr10I | RCCGGY | 3 cut(s) | 647, 667, 1103 |
| Cfr13I | GGNCC | 4 cut(s) | 665, 719, 983, 1220 |
| CseI | GACGC | 2 cut(s) | 349, 928 |
| Csp6I | GTAC | 4 cut(s) | 454, 686, 789, 821 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 687, 722 |
| CviAII | CATG | 4 cut(s) | 280, 550, 683, 1318 |
| CviQI | GTAC | 4 cut(s) | 454, 686, 789, 821 |
| DdeI | CTNAG | 4 cut(s) | 30, 94, 804, 1041 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 208 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 208 |
| EaeI | YGGCCR | 3 cut(s) | 387, 540, 585 |
| Eam1104I | CTCTTC | 3 cut(s) | 118, 183, 303 |
| EarI | CTCTTC | 3 cut(s) | 118, 183, 303 |
| Ecl136II | GAGCTC | 1 cut(s) | 40 |
| Eco130I | CCWWGG | 2 cut(s) | 824, 1061 |
| Eco24I | GRGCYC | 1 cut(s) | 42 |
| Eco47I | GGWCC | 1 cut(s) | 719 |
| Eco53kI | GAGCTC | 1 cut(s) | 40 |
| Eco57I | CTGAAG | 3 cut(s) | 99, 218, 327 |
| Eco88I | CYCGRG | 1 cut(s) | 784 |
| EcoICRI | GAGCTC | 1 cut(s) | 40 |
| EcoRII | CCWGG | 7 cut(s) | 412, 435, 661, 700, 791, 924, 1028 |
| EcoT14I | CCWWGG | 2 cut(s) | 824, 1061 |
| EcoT22I | ATGCAT | 1 cut(s) | 684 |
| EcoT38I | GRGCYC | 1 cut(s) | 42 |
| ErhI | CCWWGG | 2 cut(s) | 824, 1061 |
| Esp3I | CGTCTC | 2 cut(s) | 926, 1163 |
| FaeI | CATG | 3 cut(s) | 283, 553, 686 |
| FaqI | GGGAC | 2 cut(s) | 732, 926 |
| FatI | CATG | 3 cut(s) | 279, 549, 682 |
| FauI | CCCGC | 5 cut(s) | 287, 334, 358, 969, 1206 |
| FauNDI | CATATG | 1 cut(s) | 1261 |
| FblI | GTMKAC | 1 cut(s) | 637 |
| Fnu4HI | GCNGC | 7 cut(s) | 255, 418, 421, 424, 543, 585, 622 |
| FokI | GGATG | 4 cut(s) | 317, 910, 1147, 1163 |
| FriOI | GRGCYC | 1 cut(s) | 42 |
| Fsp4HI | GCNGC | 7 cut(s) | 255, 418, 421, 424, 543, 585, 622 |
| FspBI | CTAG | 3 cut(s) | 176, 825, 1062 |
| GlaI | GCGC | 7 cut(s) | 258, 509, 620, 625, 646, 830, 1067 |
| GluI | GCNGC | 7 cut(s) | 255, 418, 421, 424, 543, 585, 622 |
| GsaI | CCCAGC | 4 cut(s) | 268, 645, 886, 1123 |
| GsuI | CTGGAG | 2 cut(s) | 929, 1166 |
| HaeII | RGCGCY | 2 cut(s) | 260, 648 |
| HgaI | GACGC | 2 cut(s) | 349, 928 |
| HhaI | GCGC | 7 cut(s) | 259, 510, 621, 626, 647, 831, 1068 |
| Hin1I | GRCGYC | 3 cut(s) | 360, 939, 1184 |
| Hin1II | CATG | 3 cut(s) | 283, 553, 686 |
| Hin6I | GCGC | 7 cut(s) | 257, 508, 619, 624, 645, 829, 1066 |
| HinP1I | GCGC | 7 cut(s) | 257, 508, 619, 624, 645, 829, 1066 |
| HincII | GTYRAC | 2 cut(s) | 638, 1188 |
| HindII | GTYRAC | 2 cut(s) | 638, 1188 |
| HinfI | GANTC | 3 cut(s) | 761, 860, 1097 |
| HphI | GGTGA | 4 cut(s) | 413, 439, 821, 1058 |
| Hpy166II | GTNNAC | 5 cut(s) | 638, 969, 1188, 1206, 1315 |
| Hpy188I | TCNGA | 4 cut(s) | 33, 346, 957, 1194 |
| Hpy188III | TCNNGA | 6 cut(s) | 550, 711, 943, 975, 1180, 1212 |
| Hpy8I | GTNNAC | 5 cut(s) | 638, 969, 1188, 1206, 1315 |
| Hpy99I | CGWCG | 3 cut(s) | 208, 251, 1186 |
| HpyAV | CCTTC | 2 cut(s) | 178, 511 |
| HpyCH4III | ACNGT | 2 cut(s) | 75, 208 |
| HpyCH4IV | ACGT | 5 cut(s) | 231, 452, 556, 631, 1184 |
| HpyCH4V | TGCA | 8 cut(s) | 277, 682, 875, 901, 1112, 1138, 1243, 1265 |
| HpyF3I | CTNAG | 4 cut(s) | 30, 94, 804, 1041 |
| HpySE526I | ACGT | 5 cut(s) | 231, 452, 556, 631, 1184 |
| Hsp92I | GRCGYC | 3 cut(s) | 360, 939, 1184 |
| Hsp92II | CATG | 3 cut(s) | 283, 553, 686 |
| HspAI | GCGC | 7 cut(s) | 257, 508, 619, 624, 645, 829, 1066 |
| KroI | GCCGGC | 2 cut(s) | 647, 1103 |
| KroNI | GCCGGC | 2 cut(s) | 649, 1105 |
| LguI | GCTCTTC | 1 cut(s) | 303 |
| LmnI | GCTCC | 3 cut(s) | 505, 910, 1147 |
| Lsp1109I | GCAGC | 3 cut(s) | 429, 432, 633 |
| LweI | GCATC | 6 cut(s) | 648, 862, 888, 1099, 1125, 1185 |
| MaeI | CTAG | 3 cut(s) | 176, 825, 1062 |
| MaeII | ACGT | 5 cut(s) | 231, 452, 556, 631, 1184 |
| MaeIII | GTNAC | 1 cut(s) | 445 |
| MbiI | CCGCTC | 2 cut(s) | 783, 1020 |
| MboII | GAAGA | 6 cut(s) | 99, 105, 200, 211, 320, 467 |
| MfeI | CAATTG | 1 cut(s) | 183 |
| MflI | RGATCY | 5 cut(s) | 143, 409, 439, 472, 1236 |
| MhlI | GDGCHC | 4 cut(s) | 18, 42, 140, 373 |
| MluCI | AATT | 5 cut(s) | 21, 53, 183, 271, 566 |
| MmeI | TCCRAC | 1 cut(s) | 369 |
| Mph1103I | ATGCAT | 1 cut(s) | 684 |
| MreI | CGCCGGCG | 1 cut(s) | 1103 |
| MroNI | GCCGGC | 2 cut(s) | 647, 1103 |
| MroXI | GAANNNNTTC | 1 cut(s) | 765 |
| MseI | TTAA | 3 cut(s) | 270, 569, 920 |
| MslI | CAYNNNNRTG | 3 cut(s) | 47, 656, 677 |
| MspA1I | CMGCKG | 4 cut(s) | 294, 691, 964, 1201 |
| MteI | GCGCNGCGC | 1 cut(s) | 622 |
| MunI | CAATTG | 1 cut(s) | 183 |
| Mva1269I | GAATGC | 1 cut(s) | 1030 |
| MvaI | CCWGG | 7 cut(s) | 414, 437, 663, 702, 793, 926, 1030 |
| MvnI | CGCG | 2 cut(s) | 353, 619 |
| NaeI | GCCGGC | 2 cut(s) | 649, 1105 |
| NciI | CCSGG | 7 cut(s) | 386, 396, 599, 981, 1163, 1218, 1234 |
| NdeI | CATATG | 1 cut(s) | 1261 |
| NgoMIV | GCCGGC | 2 cut(s) | 647, 1103 |
| NlaIII | CATG | 3 cut(s) | 283, 553, 686 |
| NlaIV | GGNNCC | 6 cut(s) | 85, 720, 959, 985, 1196, 1222 |
| NmeAIII | GCCGAG | 2 cut(s) | 820, 1057 |
| NmuCI | GTSAC | 1 cut(s) | 445 |
| NsiI | ATGCAT | 1 cut(s) | 684 |
| NspI | RCATGY | 1 cut(s) | 686 |
| PaeR7I | CTCGAG | 1 cut(s) | 784 |
| PagI | TCATGA | 1 cut(s) | 549 |
| PciSI | GCTCTTC | 1 cut(s) | 303 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 224, 941, 1178 |
| PctI | GAATGC | 1 cut(s) | 1030 |
| PdiI | GCCGGC | 2 cut(s) | 649, 1105 |
| PdmI | GAANNNNTTC | 1 cut(s) | 765 |
| PfeI | GAWTC | 3 cut(s) | 761, 860, 1097 |
| Pfl23II | CGTACG | 1 cut(s) | 453 |
| PflFI | GACNNNGTC | 2 cut(s) | 208, 633 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 889, 1126 |
| PkrI | GCNGC | 7 cut(s) | 256, 419, 422, 425, 544, 586, 623 |
| Ple19I | CGATCG | 1 cut(s) | 357 |
| Ppu21I | YACGTR | 1 cut(s) | 453 |
| PshAI | GACNNNNGTC | 1 cut(s) | 938 |
| Psp124BI | GAGCTC | 1 cut(s) | 42 |
| Psp6I | CCWGG | 7 cut(s) | 412, 435, 661, 700, 791, 924, 1028 |
| PspFI | CCCAGC | 4 cut(s) | 264, 641, 882, 1119 |
| PspGI | CCWGG | 7 cut(s) | 412, 435, 661, 700, 791, 924, 1028 |
| PspLI | CGTACG | 1 cut(s) | 453 |
| PspN4I | GGNNCC | 6 cut(s) | 85, 720, 959, 985, 1196, 1222 |
| PspPI | GGNCC | 4 cut(s) | 665, 719, 983, 1220 |
| PspXI | VCTCGAGB | 1 cut(s) | 784 |
| PsuI | RGATCY | 5 cut(s) | 143, 409, 439, 472, 1236 |
| PsyI | GACNNNGTC | 2 cut(s) | 208, 633 |
| PvuI | CGATCG | 1 cut(s) | 357 |
| RsaI | GTAC | 4 cut(s) | 455, 687, 790, 822 |
| RsaNI | GTAC | 4 cut(s) | 454, 686, 789, 821 |
| RseI | CAYNNNNRTG | 3 cut(s) | 47, 656, 677 |
| SacI | GAGCTC | 1 cut(s) | 42 |
| SalI | GTCGAC | 1 cut(s) | 636 |
| SapI | GCTCTTC | 1 cut(s) | 303 |
| SaqAI | TTAA | 3 cut(s) | 270, 569, 920 |
| SatI | GCNGC | 7 cut(s) | 255, 418, 421, 424, 543, 585, 622 |
| Sau96I | GGNCC | 4 cut(s) | 665, 719, 983, 1220 |
| SduI | GDGCHC | 4 cut(s) | 18, 42, 140, 373 |
| SfaNI | GCATC | 6 cut(s) | 648, 862, 888, 1099, 1125, 1185 |
| Sfr274I | CTCGAG | 1 cut(s) | 784 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1103 |
| SinI | GGWCC | 1 cut(s) | 719 |
| SlaI | CTCGAG | 1 cut(s) | 784 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 47, 656, 677 |
| SmlI | CTYRAG | 1 cut(s) | 784 |
| SmoI | CTYRAG | 1 cut(s) | 784 |
| Sse9I | AATT | 5 cut(s) | 21, 53, 183, 271, 566 |
| SspMI | CTAG | 3 cut(s) | 176, 825, 1062 |
| SstI | GAGCTC | 1 cut(s) | 42 |
| StyI | CCWWGG | 2 cut(s) | 824, 1061 |
| TaaI | ACNGT | 2 cut(s) | 75, 208 |
| TaiI | ACGT | 5 cut(s) | 234, 455, 559, 634, 1187 |
| TaqI | TCGA | 3 cut(s) | 637, 785, 1022 |
| TaqII | GACCGA | 1 cut(s) | 1316 |
| TasI | AATT | 5 cut(s) | 21, 53, 183, 271, 566 |
| TauI | GCSGC | 4 cut(s) | 257, 426, 545, 587 |
| TfiI | GAWTC | 3 cut(s) | 761, 860, 1097 |
| Tru1I | TTAA | 3 cut(s) | 270, 569, 920 |
| Tru9I | TTAA | 3 cut(s) | 270, 569, 920 |
| TscAI | CASTG | 1 cut(s) | 471 |
| TseFI | GTSAC | 1 cut(s) | 445 |
| TseI | GCWGC | 3 cut(s) | 417, 420, 621 |
| Tsp45I | GTSAC | 1 cut(s) | 445 |
| TspDTI | ATGAA | 2 cut(s) | 93, 467 |
| TspGWI | ACGGA | 1 cut(s) | 332 |
| TspRI | CASTG | 1 cut(s) | 471 |
| Tth111I | GACNNNGTC | 2 cut(s) | 208, 633 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 889, 1126 |
| VpaK11BI | GGWCC | 1 cut(s) | 719 |
| XapI | RAATTY | 1 cut(s) | 53 |
| XceI | RCATGY | 1 cut(s) | 686 |
| XhoI | CTCGAG | 1 cut(s) | 784 |
| XmaJI | CCTAGG | 2 cut(s) | 824, 1061 |
| XmiI | GTMKAC | 1 cut(s) | 637 |
| XmnI | GAANNNNTTC | 1 cut(s) | 765 |
| XspI | CTAG | 3 cut(s) | 176, 825, 1062 |
| ZraI | GACGTC | 1 cut(s) | 1185 |
| Zsp2I | ATGCAT | 1 cut(s) | 684 |