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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 439, 466 |
| AccB1I | GGYRCC | 2 cut(s) | 439, 466 |
| AccI | GTMKAC | 1 cut(s) | 418 |
| AccII | CGCG | 1 cut(s) | 74 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AcoI | YGGCCR | 1 cut(s) | 321 |
| AfaI | GTAC | 2 cut(s) | 441, 468 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 139, 262, 428, 573 |
| AgsI | TTSAA | 2 cut(s) | 118, 475 |
| AjnI | CCWGG | 6 cut(s) | 147, 159, 211, 216, 407, 572 |
| AluBI | AGCT | 4 cut(s) | 201, 230, 241, 487 |
| AluI | AGCT | 4 cut(s) | 201, 230, 241, 487 |
| Alw21I | GWGCWC | 2 cut(s) | 112, 407 |
| Alw44I | GTGCAC | 1 cut(s) | 108 |
| AoxI | GGCC | 4 cut(s) | 214, 306, 321, 499 |
| ApaLI | GTGCAC | 1 cut(s) | 108 |
| ApeKI | GCWGC | 3 cut(s) | 198, 227, 569 |
| Asp718I | GGTACC | 2 cut(s) | 439, 466 |
| AspLEI | GCGC | 1 cut(s) | 385 |
| AspS9I | GGNCC | 4 cut(s) | 163, 220, 253, 500 |
| AvaII | GGWCC | 3 cut(s) | 163, 220, 253 |
| BaeGI | GKGCMC | 1 cut(s) | 112 |
| BalI | TGGCCA | 1 cut(s) | 323 |
| BanI | GGYRCC | 2 cut(s) | 439, 466 |
| Bbv12I | GWGCWC | 2 cut(s) | 112, 407 |
| BbvCI | CCTCAGC | 1 cut(s) | 303 |
| BbvI | GCAGC | 3 cut(s) | 210, 239, 581 |
| BccI | CCATC | 2 cut(s) | 66, 487 |
| BciT130I | CCWGG | 6 cut(s) | 149, 161, 213, 218, 409, 574 |
| BciVI | GTATCC | 1 cut(s) | 145 |
| BfaI | CTAG | 4 cut(s) | 89, 135, 194, 579 |
| BfmI | CTRYAG | 1 cut(s) | 177 |
| BfuI | GTATCC | 1 cut(s) | 145 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 213 |
| BisI | GCNGC | 3 cut(s) | 199, 228, 570 |
| BlsI | GCNGC | 3 cut(s) | 200, 229, 571 |
| Bme1390I | CCNGG | 6 cut(s) | 149, 161, 213, 218, 409, 574 |
| Bme18I | GGWCC | 3 cut(s) | 163, 220, 253 |
| BmgT120I | GGNCC | 4 cut(s) | 163, 220, 253, 500 |
| BmiI | GGNNCC | 4 cut(s) | 144, 165, 441, 468 |
| BmrFI | CCNGG | 6 cut(s) | 149, 161, 213, 218, 409, 574 |
| BpmI | CTGGAG | 1 cut(s) | 383 |
| Bpu10I | CCTNAGC | 1 cut(s) | 303 |
| BsaJI | CCNNGG | 3 cut(s) | 147, 159, 573 |
| BsaWI | WCCGGW | 1 cut(s) | 124 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 139, 262, 428, 573 |
| Bse118I | RCCGGY | 1 cut(s) | 428 |
| Bse1I | ACTGG | 1 cut(s) | 43 |
| BseBI | CCWGG | 6 cut(s) | 149, 161, 213, 218, 409, 574 |
| BseDI | CCNNGG | 3 cut(s) | 147, 159, 573 |
| BseGI | GGATG | 2 cut(s) | 58, 479 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 139, 262, 428, 573 |
| BseMII | CTCAG | 1 cut(s) | 294 |
| BseNI | ACTGG | 1 cut(s) | 43 |
| BseSI | GKGCMC | 1 cut(s) | 112 |
| BseXI | GCAGC | 3 cut(s) | 210, 239, 581 |
| BseYI | CCCAGC | 1 cut(s) | 379 |
| Bsh1236I | CGCG | 1 cut(s) | 74 |
| BshFI | GGCC | 4 cut(s) | 216, 308, 323, 501 |
| BshNI | GGYRCC | 2 cut(s) | 439, 466 |
| BsiHKAI | GWGCWC | 2 cut(s) | 112, 407 |
| BsiSI | CCGG | 2 cut(s) | 125, 429 |
| BslFI | GGGAC | 1 cut(s) | 149 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 139, 262, 428, 573 |
| BsmFI | GGGAC | 1 cut(s) | 149 |
| BsnI | GGCC | 4 cut(s) | 216, 308, 323, 501 |
| Bsp1286I | GDGCHC | 2 cut(s) | 112, 407 |
| BspANI | GGCC | 4 cut(s) | 216, 308, 323, 501 |
| BspCNI | CTCAG | 1 cut(s) | 295 |
| BspFNI | CGCG | 1 cut(s) | 74 |
| BspLI | GGNNCC | 4 cut(s) | 144, 165, 441, 468 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 2 cut(s) | 439, 466 |
| BsrFI | RCCGGY | 1 cut(s) | 428 |
| BsrI | ACTGG | 1 cut(s) | 43 |
| BssAI | RCCGGY | 1 cut(s) | 428 |
| BssECI | CCNNGG | 3 cut(s) | 147, 159, 573 |
| Bst2UI | CCWGG | 6 cut(s) | 149, 161, 213, 218, 409, 574 |
| Bst4CI | ACNGT | 2 cut(s) | 107, 471 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 243, 392, 430 |
| BstDEI | CTNAG | 1 cut(s) | 303 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 260 |
| BstF5I | GGATG | 2 cut(s) | 58, 479 |
| BstFNI | CGCG | 1 cut(s) | 74 |
| BstHHI | GCGC | 1 cut(s) | 385 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 204, 213, 380, 391, 575 |
| BstNI | CCWGG | 6 cut(s) | 149, 161, 213, 218, 409, 574 |
| BstSCI | CCNGG | 6 cut(s) | 147, 159, 211, 216, 407, 572 |
| BstSFI | CTRYAG | 1 cut(s) | 177 |
| BstSLI | GKGCMC | 1 cut(s) | 112 |
| BstUI | CGCG | 1 cut(s) | 74 |
| BstV1I | GCAGC | 3 cut(s) | 210, 239, 581 |
| BsuI | GTATCC | 1 cut(s) | 145 |
| BsuRI | GGCC | 4 cut(s) | 216, 308, 323, 501 |
| BtsCI | GGATG | 2 cut(s) | 58, 479 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 243, 392, 430 |
| CfoI | GCGC | 1 cut(s) | 385 |
| Cfr10I | RCCGGY | 1 cut(s) | 428 |
| Cfr13I | GGNCC | 4 cut(s) | 163, 220, 253, 500 |
| Csp6I | GTAC | 2 cut(s) | 440, 467 |
| CviAII | CATG | 1 cut(s) | 139 |
| CviQI | GTAC | 2 cut(s) | 440, 467 |
| DdeI | CTNAG | 1 cut(s) | 303 |
| EaeI | YGGCCR | 1 cut(s) | 321 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| Eco47I | GGWCC | 3 cut(s) | 163, 220, 253 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 260 |
| EcoO109I | RGGNCCY | 2 cut(s) | 163, 220 |
| EcoRII | CCWGG | 6 cut(s) | 147, 159, 211, 216, 407, 572 |
| FaeI | CATG | 1 cut(s) | 142 |
| FaiI | YATR | 5 cut(s) | 140, 233, 237, 455, 586 |
| FaqI | GGGAC | 1 cut(s) | 149 |
| FatI | CATG | 1 cut(s) | 138 |
| FauI | CCCGC | 5 cut(s) | 8, 79, 174, 451, 519 |
| FblI | GTMKAC | 1 cut(s) | 418 |
| Fnu4HI | GCNGC | 3 cut(s) | 199, 228, 570 |
| FokI | GGATG | 2 cut(s) | 45, 466 |
| Fsp4HI | GCNGC | 3 cut(s) | 199, 228, 570 |
| FspBI | CTAG | 4 cut(s) | 89, 135, 194, 579 |
| GlaI | GCGC | 1 cut(s) | 384 |
| GluI | GCNGC | 3 cut(s) | 199, 228, 570 |
| GsaI | CCCAGC | 1 cut(s) | 383 |
| GsuI | CTGGAG | 1 cut(s) | 383 |
| HaeIII | GGCC | 4 cut(s) | 216, 308, 323, 501 |
| HapII | CCGG | 2 cut(s) | 125, 429 |
| HhaI | GCGC | 1 cut(s) | 385 |
| Hin1II | CATG | 1 cut(s) | 142 |
| Hin6I | GCGC | 1 cut(s) | 383 |
| HinP1I | GCGC | 1 cut(s) | 383 |
| HincII | GTYRAC | 4 cut(s) | 85, 97, 130, 419 |
| HindII | GTYRAC | 4 cut(s) | 85, 97, 130, 419 |
| HinfI | GANTC | 2 cut(s) | 339, 542 |
| HpaI | GTTAAC | 2 cut(s) | 85, 97 |
| HpaII | CCGG | 2 cut(s) | 125, 429 |
| Hpy166II | GTNNAC | 6 cut(s) | 85, 97, 110, 130, 281, 419 |
| Hpy188I | TCNGA | 1 cut(s) | 484 |
| Hpy188III | TCNNGA | 1 cut(s) | 336 |
| Hpy8I | GTNNAC | 6 cut(s) | 85, 97, 110, 130, 281, 419 |
| HpyCH4III | ACNGT | 2 cut(s) | 107, 471 |
| HpyCH4IV | ACGT | 1 cut(s) | 81 |
| HpyCH4V | TGCA | 1 cut(s) | 110 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 204, 213, 380, 391, 575 |
| HpyF3I | CTNAG | 1 cut(s) | 303 |
| HpySE526I | ACGT | 1 cut(s) | 81 |
| Hsp92II | CATG | 1 cut(s) | 142 |
| HspAI | GCGC | 1 cut(s) | 383 |
| KpnI | GGTACC | 2 cut(s) | 443, 470 |
| KroI | GCCGGC | 1 cut(s) | 428 |
| KroNI | GCCGGC | 1 cut(s) | 430 |
| KspAI | GTTAAC | 2 cut(s) | 85, 97 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 569 |
| Lsp1109I | GCAGC | 3 cut(s) | 210, 239, 581 |
| MaeI | CTAG | 4 cut(s) | 89, 135, 194, 579 |
| MaeII | ACGT | 1 cut(s) | 81 |
| MaeIII | GTNAC | 1 cut(s) | 543 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MhlI | GDGCHC | 2 cut(s) | 112, 407 |
| MlsI | TGGCCA | 1 cut(s) | 323 |
| MluNI | TGGCCA | 1 cut(s) | 323 |
| MlyI | GAGTC | 1 cut(s) | 551 |
| MnlI | CCTC | 6 cut(s) | 178, 233, 266, 298, 358, 516 |
| Mox20I | TGGCCA | 1 cut(s) | 323 |
| MroNI | GCCGGC | 1 cut(s) | 428 |
| MscI | TGGCCA | 1 cut(s) | 323 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| Msp20I | TGGCCA | 1 cut(s) | 323 |
| MspA1I | CMGCKG | 2 cut(s) | 182, 201 |
| MspI | CCGG | 2 cut(s) | 125, 429 |
| MspR9I | CCNGG | 6 cut(s) | 149, 161, 213, 218, 409, 574 |
| MvaI | CCWGG | 6 cut(s) | 149, 161, 213, 218, 409, 574 |
| MvnI | CGCG | 1 cut(s) | 74 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 204, 213, 380, 391, 575 |
| NaeI | GCCGGC | 1 cut(s) | 430 |
| NgoMIV | GCCGGC | 1 cut(s) | 428 |
| NlaIII | CATG | 1 cut(s) | 142 |
| NlaIV | GGNNCC | 4 cut(s) | 144, 165, 441, 468 |
| NmeAIII | GCCGAG | 1 cut(s) | 169 |
| NmuCI | GTSAC | 1 cut(s) | 543 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PdiI | GCCGGC | 1 cut(s) | 430 |
| PfeI | GAWTC | 1 cut(s) | 339 |
| PkrI | GCNGC | 3 cut(s) | 200, 229, 571 |
| PleI | GAGTC | 1 cut(s) | 550 |
| PpsI | GAGTC | 1 cut(s) | 550 |
| PpuMI | RGGWCCY | 2 cut(s) | 163, 220 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| Psp5II | RGGWCCY | 2 cut(s) | 163, 220 |
| Psp6I | CCWGG | 6 cut(s) | 147, 159, 211, 216, 407, 572 |
| PspFI | CCCAGC | 1 cut(s) | 379 |
| PspGI | CCWGG | 6 cut(s) | 147, 159, 211, 216, 407, 572 |
| PspN4I | GGNNCC | 4 cut(s) | 144, 165, 441, 468 |
| PspPI | GGNCC | 4 cut(s) | 163, 220, 253, 500 |
| PspPPI | RGGWCCY | 2 cut(s) | 163, 220 |
| PvuII | CAGCTG | 1 cut(s) | 201 |
| RsaI | GTAC | 2 cut(s) | 441, 468 |
| RsaNI | GTAC | 2 cut(s) | 440, 467 |
| SalI | GTCGAC | 1 cut(s) | 417 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 3 cut(s) | 199, 228, 570 |
| Sau96I | GGNCC | 4 cut(s) | 163, 220, 253, 500 |
| SchI | GAGTC | 1 cut(s) | 551 |
| ScrFI | CCNGG | 6 cut(s) | 149, 161, 213, 218, 409, 574 |
| SduI | GDGCHC | 2 cut(s) | 112, 407 |
| SfcI | CTRYAG | 1 cut(s) | 177 |
| SinI | GGWCC | 3 cut(s) | 163, 220, 253 |
| SspMI | CTAG | 4 cut(s) | 89, 135, 194, 579 |
| StyD4I | CCNGG | 6 cut(s) | 147, 159, 211, 216, 407, 572 |
| TaaI | ACNGT | 2 cut(s) | 107, 471 |
| TaiI | ACGT | 1 cut(s) | 84 |
| TaqI | TCGA | 2 cut(s) | 342, 418 |
| TfiI | GAWTC | 1 cut(s) | 339 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TseFI | GTSAC | 1 cut(s) | 543 |
| TseI | GCWGC | 3 cut(s) | 198, 227, 569 |
| Tsp45I | GTSAC | 1 cut(s) | 543 |
| TspDTI | ATGAA | 1 cut(s) | 360 |
| VneI | GTGCAC | 1 cut(s) | 108 |
| VpaK11BI | GGWCC | 3 cut(s) | 163, 220, 253 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 260 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 316 |
| XmiI | GTMKAC | 1 cut(s) | 418 |
| XspI | CTAG | 4 cut(s) | 89, 135, 194, 579 |