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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 281 |
| Acc36I | ACCTGC | 2 cut(s) | 281, 374 |
| AccB1I | GGYRCC | 1 cut(s) | 197 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 428 |
| AccBSI | CCGCTC | 1 cut(s) | 322 |
| AccI | GTMKAC | 1 cut(s) | 176 |
| AccII | CGCG | 3 cut(s) | 70, 158, 564 |
| AclWI | GGATC | 4 cut(s) | 148, 345, 455, 560 |
| AcuI | CTGAAG | 1 cut(s) | 28 |
| AcyI | GRCGYC | 2 cut(s) | 198, 513 |
| AfaI | GTAC | 3 cut(s) | 191, 214, 226 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 57, 186, 337, 364, 370, 428 |
| AjnI | CCWGG | 7 cut(s) | 113, 136, 165, 200, 239, 330, 363 |
| AluBI | AGCT | 1 cut(s) | 425 |
| AluI | AGCT | 1 cut(s) | 425 |
| Alw26I | GTCTC | 2 cut(s) | 465, 492 |
| AlwI | GGATC | 4 cut(s) | 148, 345, 455, 560 |
| AoxI | GGCC | 5 cut(s) | 134, 204, 372, 394, 548 |
| ApeKI | GCWGC | 3 cut(s) | 118, 121, 160 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 39, 71 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 304 |
| AspLEI | GCGC | 4 cut(s) | 72, 160, 200, 370 |
| AspS9I | GGNCC | 4 cut(s) | 204, 258, 340, 549 |
| AsuC2I | CCSGG | 5 cut(s) | 97, 465, 483, 510, 547 |
| AsuHPI | GGTGA | 2 cut(s) | 114, 360 |
| AvaII | GGWCC | 2 cut(s) | 258, 340 |
| BanI | GGYRCC | 1 cut(s) | 197 |
| BbvI | GCAGC | 3 cut(s) | 130, 133, 172 |
| BccI | CCATC | 1 cut(s) | 39 |
| BciT130I | CCWGG | 7 cut(s) | 115, 138, 167, 202, 241, 332, 365 |
| BcnI | CCSGG | 5 cut(s) | 97, 465, 483, 510, 547 |
| BcoDI | GTCTC | 2 cut(s) | 465, 492 |
| BfmI | CTRYAG | 1 cut(s) | 570 |
| BfoI | RGCGCY | 1 cut(s) | 201 |
| BfuAI | ACCTGC | 2 cut(s) | 281, 374 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 133 |
| BisI | GCNGC | 5 cut(s) | 119, 122, 125, 161, 562 |
| BlsI | GCNGC | 5 cut(s) | 120, 123, 126, 162, 563 |
| Bme18I | GGWCC | 2 cut(s) | 258, 340 |
| BmgT120I | GGNCC | 4 cut(s) | 204, 258, 340, 549 |
| BmiI | GGNNCC | 4 cut(s) | 199, 259, 525, 551 |
| BmsI | GCATC | 3 cut(s) | 401, 427, 495 |
| BoxI | GACNNNNGTC | 2 cut(s) | 477, 504 |
| BpmI | CTGGAG | 1 cut(s) | 468 |
| BpuMI | CCSGG | 5 cut(s) | 97, 465, 483, 510, 547 |
| BsaHI | GRCGYC | 2 cut(s) | 198, 513 |
| BsaJI | CCNNGG | 5 cut(s) | 95, 201, 240, 331, 363 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 441, 471 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 57, 186, 337, 364, 370, 428 |
| Bse118I | RCCGGY | 2 cut(s) | 186, 206 |
| Bse1I | ACTGG | 1 cut(s) | 451 |
| BseBI | CCWGG | 7 cut(s) | 115, 138, 167, 202, 241, 332, 365 |
| BseDI | CCNNGG | 5 cut(s) | 95, 201, 240, 331, 363 |
| BseGI | GGATG | 2 cut(s) | 31, 442 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 57, 186, 337, 364, 370, 428 |
| BseMII | CTCAG | 2 cut(s) | 158, 393 |
| BseNI | ACTGG | 1 cut(s) | 451 |
| BseXI | GCAGC | 3 cut(s) | 130, 133, 172 |
| BseYI | CCCAGC | 2 cut(s) | 37, 421 |
| Bsh1236I | CGCG | 3 cut(s) | 70, 158, 564 |
| Bsh1285I | CGRYCG | 1 cut(s) | 211 |
| BshFI | GGCC | 5 cut(s) | 136, 206, 374, 396, 550 |
| BshNI | GGYRCC | 1 cut(s) | 197 |
| BsiEI | CGRYCG | 1 cut(s) | 211 |
| BsiSI | CCGG | 7 cut(s) | 97, 187, 207, 464, 483, 510, 547 |
| BslFI | GGGAC | 3 cut(s) | 271, 465, 492 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 57, 186, 337, 364, 370, 428 |
| BsmAI | GTCTC | 2 cut(s) | 465, 492 |
| BsmFI | GGGAC | 3 cut(s) | 271, 465, 492 |
| BsmI | GAATGC | 2 cut(s) | 54, 332 |
| BsnI | GGCC | 5 cut(s) | 136, 206, 374, 396, 550 |
| Bsp143I | GATC | 4 cut(s) | 140, 350, 460, 565 |
| BspANI | GGCC | 5 cut(s) | 136, 206, 374, 396, 550 |
| BspCNI | CTCAG | 2 cut(s) | 157, 392 |
| BspFNI | CGCG | 3 cut(s) | 70, 158, 564 |
| BspLI | GGNNCC | 4 cut(s) | 199, 259, 525, 551 |
| BspMAI | CTGCAG | 1 cut(s) | 574 |
| BspMI | ACCTGC | 2 cut(s) | 281, 374 |
| BspPI | GGATC | 4 cut(s) | 148, 345, 455, 560 |
| BspQI | GCTCTTC | 1 cut(s) | 4 |
| BspT107I | GGYRCC | 1 cut(s) | 197 |
| BsrBI | CCGCTC | 1 cut(s) | 322 |
| BsrFI | RCCGGY | 2 cut(s) | 186, 206 |
| BsrI | ACTGG | 1 cut(s) | 451 |
| BssAI | RCCGGY | 2 cut(s) | 186, 206 |
| BssECI | CCNNGG | 5 cut(s) | 95, 201, 240, 331, 363 |
| BssMI | GATC | 4 cut(s) | 140, 350, 460, 565 |
| BssNI | GRCGYC | 2 cut(s) | 198, 513 |
| Bst2UI | CCWGG | 7 cut(s) | 115, 138, 167, 202, 241, 332, 365 |
| Bst6I | CTCTTC | 1 cut(s) | 4 |
| BstACI | GRCGYC | 2 cut(s) | 198, 513 |
| BstC8I | GCNNGC | 4 cut(s) | 16, 150, 372, 574 |
| BstDEI | CTNAG | 3 cut(s) | 144, 379, 493 |
| BstF5I | GGATG | 2 cut(s) | 31, 442 |
| BstFNI | CGCG | 3 cut(s) | 70, 158, 564 |
| BstH2I | RGCGCY | 1 cut(s) | 201 |
| BstHHI | GCGC | 4 cut(s) | 72, 160, 200, 370 |
| BstKTI | GATC | 4 cut(s) | 143, 353, 463, 568 |
| BstMAI | GTCTC | 2 cut(s) | 465, 492 |
| BstMBI | GATC | 4 cut(s) | 140, 350, 460, 565 |
| BstMCI | CGRYCG | 1 cut(s) | 211 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 124, 133, 227, 380, 402, 411 |
| BstNI | CCWGG | 7 cut(s) | 115, 138, 167, 202, 241, 332, 365 |
| BstNSI | RCATGY | 1 cut(s) | 225 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 477, 504 |
| BstSFI | CTRYAG | 1 cut(s) | 570 |
| BstUI | CGCG | 3 cut(s) | 70, 158, 564 |
| BstV1I | GCAGC | 3 cut(s) | 130, 133, 172 |
| BstX2I | RGATCY | 1 cut(s) | 140 |
| BstYI | RGATCY | 1 cut(s) | 140 |
| BsuRI | GGCC | 5 cut(s) | 136, 206, 374, 396, 550 |
| BtsCI | GGATG | 2 cut(s) | 31, 442 |
| BveI | ACCTGC | 2 cut(s) | 281, 374 |
| Cac8I | GCNNGC | 4 cut(s) | 16, 150, 372, 574 |
| CfoI | GCGC | 4 cut(s) | 72, 160, 200, 370 |
| Cfr10I | RCCGGY | 2 cut(s) | 186, 206 |
| Cfr13I | GGNCC | 4 cut(s) | 204, 258, 340, 549 |
| CseI | GACGC | 1 cut(s) | 502 |
| Csp6I | GTAC | 3 cut(s) | 190, 213, 225 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 226, 261 |
| CviAII | CATG | 1 cut(s) | 222 |
| CviQI | GTAC | 3 cut(s) | 190, 213, 225 |
| DdeI | CTNAG | 3 cut(s) | 144, 379, 493 |
| DinI | GGCGCC | 1 cut(s) | 199 |
| DpnI | GATC | 4 cut(s) | 142, 352, 462, 567 |
| DpnII | GATC | 4 cut(s) | 140, 350, 460, 565 |
| Eam1104I | CTCTTC | 1 cut(s) | 4 |
| EarI | CTCTTC | 1 cut(s) | 4 |
| EciI | GGCGGA | 4 cut(s) | 312, 343, 392, 483 |
| Eco47I | GGWCC | 2 cut(s) | 258, 340 |
| Eco57I | CTGAAG | 1 cut(s) | 28 |
| EcoRII | CCWGG | 7 cut(s) | 113, 136, 165, 200, 239, 330, 363 |
| EcoT22I | ATGCAT | 1 cut(s) | 223 |
| EgeI | GGCGCC | 1 cut(s) | 199 |
| EheI | GGCGCC | 1 cut(s) | 199 |
| FaeI | CATG | 1 cut(s) | 225 |
| FaiI | YATR | 5 cut(s) | 223, 270, 360, 590, 592 |
| FaqI | GGGAC | 3 cut(s) | 271, 465, 492 |
| FatI | CATG | 1 cut(s) | 221 |
| FauI | CCCGC | 2 cut(s) | 35, 535 |
| FauNDI | CATATG | 1 cut(s) | 590 |
| FblI | GTMKAC | 1 cut(s) | 176 |
| Fnu4HI | GCNGC | 5 cut(s) | 119, 122, 125, 161, 562 |
| FokI | GGATG | 2 cut(s) | 18, 449 |
| Fsp4HI | GCNGC | 5 cut(s) | 119, 122, 125, 161, 562 |
| GlaI | GCGC | 4 cut(s) | 71, 159, 199, 369 |
| GluI | GCNGC | 5 cut(s) | 119, 122, 125, 161, 562 |
| GsaI | CCCAGC | 2 cut(s) | 41, 425 |
| GsuI | CTGGAG | 1 cut(s) | 468 |
| HaeII | RGCGCY | 1 cut(s) | 201 |
| HaeIII | GGCC | 5 cut(s) | 136, 206, 374, 396, 550 |
| HapII | CCGG | 7 cut(s) | 97, 187, 207, 464, 483, 510, 547 |
| HgaI | GACGC | 1 cut(s) | 502 |
| HhaI | GCGC | 4 cut(s) | 72, 160, 200, 370 |
| Hin1I | GRCGYC | 2 cut(s) | 198, 513 |
| Hin1II | CATG | 1 cut(s) | 225 |
| Hin6I | GCGC | 4 cut(s) | 70, 158, 198, 368 |
| HinP1I | GCGC | 4 cut(s) | 70, 158, 198, 368 |
| HincII | GTYRAC | 3 cut(s) | 65, 177, 517 |
| HindII | GTYRAC | 3 cut(s) | 65, 177, 517 |
| HinfI | GANTC | 2 cut(s) | 300, 399 |
| HpaI | GTTAAC | 1 cut(s) | 65 |
| HpaII | CCGG | 7 cut(s) | 97, 187, 207, 464, 483, 510, 547 |
| HphI | GGTGA | 2 cut(s) | 114, 360 |
| Hpy166II | GTNNAC | 3 cut(s) | 65, 177, 517 |
| Hpy188I | TCNGA | 4 cut(s) | 47, 60, 523, 582 |
| Hpy188III | TCNNGA | 3 cut(s) | 80, 250, 541 |
| Hpy8I | GTNNAC | 3 cut(s) | 65, 177, 517 |
| Hpy99I | CGWCG | 1 cut(s) | 411 |
| HpyAV | CCTTC | 1 cut(s) | 339 |
| HpyCH4V | TGCA | 5 cut(s) | 54, 221, 414, 440, 572 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 124, 133, 227, 380, 402, 411 |
| HpyF3I | CTNAG | 3 cut(s) | 144, 379, 493 |
| Hsp92I | GRCGYC | 2 cut(s) | 198, 513 |
| Hsp92II | CATG | 1 cut(s) | 225 |
| HspAI | GCGC | 4 cut(s) | 70, 158, 198, 368 |
| KasI | GGCGCC | 1 cut(s) | 197 |
| KspAI | GTTAAC | 1 cut(s) | 65 |
| Kzo9I | GATC | 4 cut(s) | 140, 350, 460, 565 |
| LguI | GCTCTTC | 1 cut(s) | 4 |
| LmnI | GCTCC | 1 cut(s) | 449 |
| Lsp1109I | GCAGC | 3 cut(s) | 130, 133, 172 |
| LweI | GCATC | 3 cut(s) | 401, 427, 495 |
| MalI | GATC | 4 cut(s) | 142, 352, 462, 567 |
| MbiI | CCGCTC | 1 cut(s) | 322 |
| MboI | GATC | 4 cut(s) | 140, 350, 460, 565 |
| MboII | GAAGA | 1 cut(s) | 21 |
| MflI | RGATCY | 1 cut(s) | 140 |
| Mly113I | GGCGCC | 1 cut(s) | 198 |
| MmeI | TCCRAC | 2 cut(s) | 70, 340 |
| MnlI | CCTC | 4 cut(s) | 53, 98, 283, 448 |
| Mph1103I | ATGCAT | 1 cut(s) | 223 |
| MroXI | GAANNNNTTC | 1 cut(s) | 304 |
| MseI | TTAA | 1 cut(s) | 64 |
| MspA1I | CMGCKG | 1 cut(s) | 230 |
| MspI | CCGG | 7 cut(s) | 97, 187, 207, 464, 483, 510, 547 |
| Mva1269I | GAATGC | 2 cut(s) | 54, 332 |
| MvaI | CCWGG | 7 cut(s) | 115, 138, 167, 202, 241, 332, 365 |
| MvnI | CGCG | 3 cut(s) | 70, 158, 564 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 124, 133, 227, 380, 402, 411 |
| NarI | GGCGCC | 1 cut(s) | 198 |
| NciI | CCSGG | 5 cut(s) | 97, 465, 483, 510, 547 |
| NdeI | CATATG | 1 cut(s) | 590 |
| NdeII | GATC | 4 cut(s) | 140, 350, 460, 565 |
| NlaIII | CATG | 1 cut(s) | 225 |
| NlaIV | GGNNCC | 4 cut(s) | 199, 259, 525, 551 |
| NsiI | ATGCAT | 1 cut(s) | 223 |
| NspI | RCATGY | 1 cut(s) | 225 |
| PaqCI | CACCTGC | 1 cut(s) | 281 |
| PciSI | GCTCTTC | 1 cut(s) | 4 |
| PctI | GAATGC | 2 cut(s) | 54, 332 |
| PdmI | GAANNNNTTC | 1 cut(s) | 304 |
| PfeI | GAWTC | 2 cut(s) | 300, 399 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 428 |
| PkrI | GCNGC | 5 cut(s) | 120, 123, 126, 162, 563 |
| PluTI | GGCGCC | 1 cut(s) | 201 |
| PshAI | GACNNNNGTC | 2 cut(s) | 477, 504 |
| Psp6I | CCWGG | 7 cut(s) | 113, 136, 165, 200, 239, 330, 363 |
| PspFI | CCCAGC | 2 cut(s) | 37, 421 |
| PspGI | CCWGG | 7 cut(s) | 113, 136, 165, 200, 239, 330, 363 |
| PspN4I | GGNNCC | 4 cut(s) | 199, 259, 525, 551 |
| PspPI | GGNCC | 4 cut(s) | 204, 258, 340, 549 |
| PstI | CTGCAG | 1 cut(s) | 574 |
| PsuI | RGATCY | 1 cut(s) | 140 |
| RsaI | GTAC | 3 cut(s) | 191, 214, 226 |
| RsaNI | GTAC | 3 cut(s) | 190, 213, 225 |
| SalI | GTCGAC | 1 cut(s) | 175 |
| SapI | GCTCTTC | 1 cut(s) | 4 |
| SaqAI | TTAA | 1 cut(s) | 64 |
| SatI | GCNGC | 5 cut(s) | 119, 122, 125, 161, 562 |
| Sau3AI | GATC | 4 cut(s) | 140, 350, 460, 565 |
| Sau96I | GGNCC | 4 cut(s) | 204, 258, 340, 549 |
| SbfI | CCTGCAGG | 1 cut(s) | 574 |
| SdaI | CCTGCAGG | 1 cut(s) | 574 |
| SetI | ASST | 9 cut(s) | 242, 275, 295, 350, 388, 427, 459, 479, 506 |
| SfaNI | GCATC | 3 cut(s) | 401, 427, 495 |
| SfcI | CTRYAG | 1 cut(s) | 570 |
| SfoI | GGCGCC | 1 cut(s) | 199 |
| SinI | GGWCC | 2 cut(s) | 258, 340 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 574 |
| SspDI | GGCGCC | 1 cut(s) | 197 |
| TaqI | TCGA | 2 cut(s) | 176, 324 |
| TaqII | GACCGA | 2 cut(s) | 357, 599 |
| TauI | GCSGC | 2 cut(s) | 127, 564 |
| TfiI | GAWTC | 2 cut(s) | 300, 399 |
| Tru1I | TTAA | 1 cut(s) | 64 |
| Tru9I | TTAA | 1 cut(s) | 64 |
| TseI | GCWGC | 3 cut(s) | 118, 121, 160 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 428 |
| VpaK11BI | GGWCC | 2 cut(s) | 258, 340 |
| XceI | RCATGY | 1 cut(s) | 225 |
| XmiI | GTMKAC | 1 cut(s) | 176 |
| XmnI | GAANNNNTTC | 1 cut(s) | 304 |
| Zsp2I | ATGCAT | 1 cut(s) | 223 |