transposition, RNA-mediated
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 589 |
| AccB1I | GGYRCC | 3 cut(s) | 232, 589, 869 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 820 |
| AccBSI | CCGCTC | 1 cut(s) | 714 |
| AccI | GTMKAC | 1 cut(s) | 568 |
| AccII | CGCG | 1 cut(s) | 120 |
| AclI | AACGTT | 1 cut(s) | 221 |
| AclWI | GGATC | 7 cut(s) | 103, 161, 187, 411, 847, 992, 1273 |
| AcoI | YGGCCR | 2 cut(s) | 321, 1151 |
| AcuI | CTGAAG | 2 cut(s) | 521, 756 |
| AcvI | CACGTG | 1 cut(s) | 1171 |
| AcyI | GRCGYC | 5 cut(s) | 233, 294, 333, 870, 1056 |
| AdeI | CACNNNGTG | 1 cut(s) | 1065 |
| AfaI | GTAC | 5 cut(s) | 19, 31, 63, 386, 591 |
| AgsI | TTSAA | 3 cut(s) | 218, 254, 1024 |
| AjiI | CACGTC | 1 cut(s) | 1139 |
| AjnI | CCWGG | 3 cut(s) | 69, 910, 1261 |
| Alw26I | GTCTC | 1 cut(s) | 1111 |
| AlwI | GGATC | 7 cut(s) | 103, 161, 187, 411, 847, 992, 1273 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 850 |
| AoxI | GGCC | 6 cut(s) | 4, 321, 364, 456, 914, 1151 |
| ApaI | GGGCCC | 1 cut(s) | 918 |
| ApeKI | GCWGC | 3 cut(s) | 351, 926, 1180 |
| Asp718I | GGTACC | 1 cut(s) | 589 |
| AspLEI | GCGC | 8 cut(s) | 122, 235, 535, 578, 754, 762, 872, 880 |
| AspS9I | GGNCC | 9 cut(s) | 4, 130, 364, 457, 650, 769, 914, 915, 1211 |
| AsuC2I | CCSGG | 5 cut(s) | 320, 330, 363, 573, 857 |
| AsuHPI | GGTGA | 5 cut(s) | 85, 90, 101, 913, 1024 |
| AvaII | GGWCC | 4 cut(s) | 130, 650, 769, 1211 |
| BaeGI | GKGCMC | 1 cut(s) | 918 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 573, 606 |
| BalI | TGGCCA | 1 cut(s) | 1153 |
| BanI | GGYRCC | 3 cut(s) | 232, 589, 869 |
| BanII | GRGCYC | 1 cut(s) | 918 |
| BbrPI | CACGTG | 1 cut(s) | 1171 |
| BbvCI | CCTCAGC | 2 cut(s) | 86, 453 |
| BbvI | GCAGC | 3 cut(s) | 363, 913, 1192 |
| BccI | CCATC | 2 cut(s) | 280, 640 |
| BceAI | ACGGC | 2 cut(s) | 220, 1000 |
| BciT130I | CCWGG | 3 cut(s) | 71, 912, 1263 |
| BciVI | GTATCC | 1 cut(s) | 1200 |
| BcnI | CCSGG | 5 cut(s) | 320, 330, 363, 573, 857 |
| BcoDI | GTCTC | 1 cut(s) | 1111 |
| BfaI | CTAG | 4 cut(s) | 558, 677, 723, 1109 |
| BfmI | CTRYAG | 2 cut(s) | 288, 1178 |
| BfoI | RGCGCY | 4 cut(s) | 236, 579, 873, 881 |
| BfuI | GTATCC | 1 cut(s) | 1200 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 363 |
| BglII | AGATCT | 1 cut(s) | 370 |
| BisI | GCNGC | 6 cut(s) | 123, 352, 355, 701, 927, 1181 |
| BlsI | GCNGC | 6 cut(s) | 124, 353, 356, 702, 928, 1182 |
| Bme1390I | CCNGG | 8 cut(s) | 71, 320, 330, 363, 573, 857, 912, 1263 |
| Bme18I | GGWCC | 4 cut(s) | 130, 650, 769, 1211 |
| BmgBI | CACGTC | 1 cut(s) | 1139 |
| BmgT120I | GGNCC | 9 cut(s) | 4, 130, 364, 457, 650, 769, 914, 915, 1211 |
| BmiI | GGNNCC | 6 cut(s) | 234, 591, 627, 651, 871, 916 |
| BmrFI | CCNGG | 8 cut(s) | 71, 320, 330, 363, 573, 857, 912, 1263 |
| BmsI | GCATC | 5 cut(s) | 99, 600, 819, 1183, 1192 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 140, 172 |
| Bpu10I | CCTNAGC | 2 cut(s) | 86, 453 |
| BpuEI | CTTGAG | 2 cut(s) | 142, 1239 |
| BpuMI | CCSGG | 5 cut(s) | 320, 330, 363, 573, 857 |
| Bsa29I | ATCGAT | 1 cut(s) | 165 |
| BsaAI | YACGTR | 2 cut(s) | 551, 1171 |
| BsaHI | GRCGYC | 5 cut(s) | 233, 294, 333, 870, 1056 |
| BsaJI | CCNNGG | 6 cut(s) | 126, 318, 328, 755, 911, 1261 |
| BsaWI | WCCGGW | 1 cut(s) | 176 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1108, 1138 |
| Bse118I | RCCGGY | 1 cut(s) | 797 |
| Bse1I | ACTGG | 2 cut(s) | 402, 627 |
| BseBI | CCWGG | 3 cut(s) | 71, 912, 1263 |
| BseCI | ATCGAT | 1 cut(s) | 165 |
| BseDI | CCNNGG | 6 cut(s) | 126, 318, 328, 755, 911, 1261 |
| BseGI | GGATG | 3 cut(s) | 272, 651, 834 |
| BseMII | CTCAG | 4 cut(s) | 100, 444, 624, 899 |
| BseNI | ACTGG | 2 cut(s) | 402, 627 |
| BseRI | GAGGAG | 2 cut(s) | 446, 1260 |
| BseSI | GKGCMC | 1 cut(s) | 918 |
| BseXI | GCAGC | 3 cut(s) | 363, 913, 1192 |
| BseYI | CCCAGC | 3 cut(s) | 283, 511, 874 |
| Bsh1236I | CGCG | 1 cut(s) | 120 |
| BshFI | GGCC | 6 cut(s) | 6, 323, 366, 458, 916, 1153 |
| BshNI | GGYRCC | 3 cut(s) | 232, 589, 869 |
| BshVI | ATCGAT | 1 cut(s) | 165 |
| BsiSI | CCGG | 7 cut(s) | 177, 320, 330, 362, 573, 798, 856 |
| BslFI | GGGAC | 3 cut(s) | 663, 1108, 1125 |
| BsmAI | GTCTC | 1 cut(s) | 1111 |
| BsmFI | GGGAC | 3 cut(s) | 663, 1108, 1125 |
| BsnI | GGCC | 6 cut(s) | 6, 323, 366, 458, 916, 1153 |
| Bsp120I | GGGCCC | 1 cut(s) | 914 |
| Bsp1286I | GDGCHC | 1 cut(s) | 918 |
| Bsp143I | GATC | 9 cut(s) | 95, 106, 166, 179, 370, 403, 852, 997, 1265 |
| BspANI | GGCC | 6 cut(s) | 6, 323, 366, 458, 916, 1153 |
| BspCNI | CTCAG | 4 cut(s) | 99, 445, 625, 898 |
| BspDI | ATCGAT | 1 cut(s) | 165 |
| BspFNI | CGCG | 1 cut(s) | 120 |
| BspLI | GGNNCC | 6 cut(s) | 234, 591, 627, 651, 871, 916 |
| BspMAI | CTGCAG | 1 cut(s) | 1182 |
| BspPI | GGATC | 7 cut(s) | 103, 161, 187, 411, 847, 992, 1273 |
| BspT107I | GGYRCC | 3 cut(s) | 232, 589, 869 |
| BsrBI | CCGCTC | 1 cut(s) | 714 |
| BsrFI | RCCGGY | 1 cut(s) | 797 |
| BsrI | ACTGG | 2 cut(s) | 402, 627 |
| BssAI | RCCGGY | 1 cut(s) | 797 |
| BssECI | CCNNGG | 6 cut(s) | 126, 318, 328, 755, 911, 1261 |
| BssMI | GATC | 9 cut(s) | 95, 106, 166, 179, 370, 403, 852, 997, 1265 |
| BssNI | GRCGYC | 5 cut(s) | 233, 294, 333, 870, 1056 |
| BssT1I | CCWWGG | 1 cut(s) | 755 |
| Bst2UI | CCWGG | 3 cut(s) | 71, 912, 1263 |
| Bst4CI | ACNGT | 3 cut(s) | 134, 468, 546 |
| Bst6I | CTCTTC | 1 cut(s) | 497 |
| BstACI | GRCGYC | 5 cut(s) | 233, 294, 333, 870, 1056 |
| BstBAI | YACGTR | 2 cut(s) | 551, 1171 |
| BstC8I | GCNNGC | 7 cut(s) | 359, 393, 580, 600, 750, 799, 939 |
| BstDEI | CTNAG | 4 cut(s) | 86, 453, 633, 885 |
| BstDSI | CCRYGG | 1 cut(s) | 126 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1200 |
| BstF5I | GGATG | 3 cut(s) | 272, 651, 834 |
| BstFNI | CGCG | 1 cut(s) | 120 |
| BstH2I | RGCGCY | 4 cut(s) | 236, 579, 873, 881 |
| BstHHI | GCGC | 8 cut(s) | 122, 235, 535, 578, 754, 762, 872, 880 |
| BstKTI | GATC | 9 cut(s) | 98, 109, 169, 182, 373, 406, 855, 1000, 1268 |
| BstMAI | GTCTC | 1 cut(s) | 1111 |
| BstMBI | GATC | 9 cut(s) | 95, 106, 166, 179, 370, 403, 852, 997, 1265 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 75, 300, 363, 530, 794, 803, 1180 |
| BstNI | CCWGG | 3 cut(s) | 71, 912, 1263 |
| BstNSI | RCATGY | 1 cut(s) | 1101 |
| BstSCI | CCNGG | 8 cut(s) | 69, 318, 328, 361, 571, 855, 910, 1261 |
| BstSFI | CTRYAG | 2 cut(s) | 288, 1178 |
| BstSLI | GKGCMC | 1 cut(s) | 918 |
| BstUI | CGCG | 1 cut(s) | 120 |
| BstV1I | GCAGC | 3 cut(s) | 363, 913, 1192 |
| BstX2I | RGATCY | 3 cut(s) | 370, 403, 1265 |
| BstYI | RGATCY | 3 cut(s) | 370, 403, 1265 |
| Bsu15I | ATCGAT | 1 cut(s) | 165 |
| BsuI | GTATCC | 1 cut(s) | 1200 |
| BsuRI | GGCC | 6 cut(s) | 6, 323, 366, 458, 916, 1153 |
| BsuTUI | ATCGAT | 1 cut(s) | 165 |
| BtgI | CCRYGG | 1 cut(s) | 126 |
| BtrI | CACGTC | 1 cut(s) | 1139 |
| BtsCI | GGATG | 3 cut(s) | 272, 651, 834 |
| BtsI | GCAGTG | 1 cut(s) | 695 |
| BtsIMutI | CAGTG | 3 cut(s) | 395, 620, 695 |
| Cac8I | GCNNGC | 7 cut(s) | 359, 393, 580, 600, 750, 799, 939 |
| CaiI | CAGNNNCTG | 1 cut(s) | 850 |
| CfoI | GCGC | 8 cut(s) | 122, 235, 535, 578, 754, 762, 872, 880 |
| Cfr10I | RCCGGY | 1 cut(s) | 797 |
| Cfr13I | GGNCC | 9 cut(s) | 4, 130, 364, 457, 650, 769, 914, 915, 1211 |
| ClaI | ATCGAT | 1 cut(s) | 165 |
| CpoI | CGGWCCG | 1 cut(s) | 130 |
| CseI | GACGC | 5 cut(s) | 126, 283, 322, 517, 1064 |
| Csp6I | GTAC | 5 cut(s) | 18, 30, 62, 385, 590 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 31 |
| CspI | CGGWCCG | 1 cut(s) | 130 |
| CviAII | CATG | 3 cut(s) | 1012, 1098, 1121 |
| CviQI | GTAC | 5 cut(s) | 18, 30, 62, 385, 590 |
| DdeI | CTNAG | 4 cut(s) | 86, 453, 633, 885 |
| DinI | GGCGCC | 2 cut(s) | 234, 871 |
| DpnI | GATC | 9 cut(s) | 97, 108, 168, 181, 372, 405, 854, 999, 1267 |
| DpnII | GATC | 9 cut(s) | 95, 106, 166, 179, 370, 403, 852, 997, 1265 |
| DraIII | CACNNNGTG | 1 cut(s) | 1065 |
| EaeI | YGGCCR | 2 cut(s) | 321, 1151 |
| Eam1104I | CTCTTC | 1 cut(s) | 497 |
| EarI | CTCTTC | 1 cut(s) | 497 |
| EciI | GGCGGA | 8 cut(s) | 159, 416, 704, 735, 875, 1002, 1182, 1230 |
| Eco130I | CCWWGG | 1 cut(s) | 755 |
| Eco24I | GRGCYC | 1 cut(s) | 918 |
| Eco47I | GGWCC | 4 cut(s) | 130, 650, 769, 1211 |
| Eco57I | CTGAAG | 2 cut(s) | 521, 756 |
| Eco72I | CACGTG | 1 cut(s) | 1171 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1200 |
| EcoO109I | RGGNCCY | 1 cut(s) | 915 |
| EcoRII | CCWGG | 3 cut(s) | 69, 910, 1261 |
| EcoT14I | CCWWGG | 1 cut(s) | 755 |
| EcoT38I | GRGCYC | 1 cut(s) | 918 |
| EgeI | GGCGCC | 2 cut(s) | 234, 871 |
| EheI | GGCGCC | 2 cut(s) | 234, 871 |
| ErhI | CCWWGG | 1 cut(s) | 755 |
| FaeI | CATG | 3 cut(s) | 1015, 1101, 1124 |
| FaiI | YATR | 8 cut(s) | 56, 383, 411, 605, 957, 1013, 1099, 1122 |
| FaqI | GGGAC | 3 cut(s) | 663, 1108, 1125 |
| FatI | CATG | 3 cut(s) | 1011, 1097, 1120 |
| FauI | CCCGC | 2 cut(s) | 366, 1150 |
| FblI | GTMKAC | 1 cut(s) | 568 |
| Fnu4HI | GCNGC | 6 cut(s) | 123, 352, 355, 701, 927, 1181 |
| FokI | GGATG | 3 cut(s) | 259, 658, 841 |
| FriOI | GRGCYC | 1 cut(s) | 918 |
| Fsp4HI | GCNGC | 6 cut(s) | 123, 352, 355, 701, 927, 1181 |
| FspBI | CTAG | 4 cut(s) | 558, 677, 723, 1109 |
| GlaI | GCGC | 8 cut(s) | 121, 234, 534, 577, 753, 761, 871, 879 |
| GluI | GCNGC | 6 cut(s) | 123, 352, 355, 701, 927, 1181 |
| GsaI | CCCAGC | 3 cut(s) | 287, 515, 878 |
| HaeII | RGCGCY | 4 cut(s) | 236, 579, 873, 881 |
| HaeIII | GGCC | 6 cut(s) | 6, 323, 366, 458, 916, 1153 |
| HapII | CCGG | 7 cut(s) | 177, 320, 330, 362, 573, 798, 856 |
| HgaI | GACGC | 5 cut(s) | 126, 283, 322, 517, 1064 |
| HhaI | GCGC | 8 cut(s) | 122, 235, 535, 578, 754, 762, 872, 880 |
| Hin1I | GRCGYC | 5 cut(s) | 233, 294, 333, 870, 1056 |
| Hin1II | CATG | 3 cut(s) | 1015, 1101, 1124 |
| Hin6I | GCGC | 8 cut(s) | 120, 233, 533, 576, 752, 760, 870, 878 |
| HinP1I | GCGC | 8 cut(s) | 120, 233, 533, 576, 752, 760, 870, 878 |
| HincII | GTYRAC | 4 cut(s) | 189, 225, 298, 569 |
| HindII | GTYRAC | 4 cut(s) | 189, 225, 298, 569 |
| HindIII | AAGCTT | 1 cut(s) | 142 |
| HinfI | GANTC | 3 cut(s) | 692, 863, 889 |
| HpaI | GTTAAC | 2 cut(s) | 189, 225 |
| HpaII | CCGG | 7 cut(s) | 177, 320, 330, 362, 573, 798, 856 |
| HphI | GGTGA | 5 cut(s) | 85, 90, 101, 913, 1024 |
| Hpy166II | GTNNAC | 8 cut(s) | 64, 189, 225, 298, 569, 664, 1009, 1214 |
| Hpy188I | TCNGA | 5 cut(s) | 95, 718, 888, 947, 997 |
| Hpy188III | TCNNGA | 3 cut(s) | 251, 906, 1256 |
| Hpy8I | GTNNAC | 8 cut(s) | 64, 189, 225, 298, 569, 664, 1009, 1214 |
| Hpy99I | CGWCG | 3 cut(s) | 120, 338, 1058 |
| HpyAV | CCTTC | 5 cut(s) | 442, 731, 928, 1012, 1030 |
| HpyCH4III | ACNGT | 3 cut(s) | 134, 468, 546 |
| HpyCH4IV | ACGT | 5 cut(s) | 221, 487, 550, 1138, 1170 |
| HpyCH4V | TGCA | 8 cut(s) | 613, 806, 832, 929, 937, 959, 1174, 1180 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 75, 300, 363, 530, 794, 803, 1180 |
| HpyF3I | CTNAG | 4 cut(s) | 86, 453, 633, 885 |
| HpySE526I | ACGT | 5 cut(s) | 221, 487, 550, 1138, 1170 |
| Hsp92I | GRCGYC | 5 cut(s) | 233, 294, 333, 870, 1056 |
| Hsp92II | CATG | 3 cut(s) | 1015, 1101, 1124 |
| HspAI | GCGC | 8 cut(s) | 120, 233, 533, 576, 752, 760, 870, 878 |
| KasI | GGCGCC | 2 cut(s) | 232, 869 |
| KpnI | GGTACC | 1 cut(s) | 593 |
| KroI | GCCGGC | 1 cut(s) | 797 |
| KroNI | GCCGGC | 1 cut(s) | 799 |
| KspAI | GTTAAC | 2 cut(s) | 189, 225 |
| Kzo9I | GATC | 9 cut(s) | 95, 106, 166, 179, 370, 403, 852, 997, 1265 |
| LmnI | GCTCC | 2 cut(s) | 515, 841 |
| Lsp1109I | GCAGC | 3 cut(s) | 363, 913, 1192 |
| LweI | GCATC | 5 cut(s) | 99, 600, 819, 1183, 1192 |
| MaeI | CTAG | 4 cut(s) | 558, 677, 723, 1109 |
| MaeII | ACGT | 5 cut(s) | 221, 487, 550, 1138, 1170 |
| MaeIII | GTNAC | 3 cut(s) | 483, 546, 1030 |
| MalI | GATC | 9 cut(s) | 97, 108, 168, 181, 372, 405, 854, 999, 1267 |
| MbiI | CCGCTC | 1 cut(s) | 714 |
| MboI | GATC | 9 cut(s) | 95, 106, 166, 179, 370, 403, 852, 997, 1265 |
| MboII | GAAGA | 3 cut(s) | 398, 514, 982 |
| MfeI | CAATTG | 1 cut(s) | 836 |
| MflI | RGATCY | 3 cut(s) | 370, 403, 1265 |
| MhlI | GDGCHC | 1 cut(s) | 918 |
| MlsI | TGGCCA | 1 cut(s) | 1153 |
| MluCI | AATT | 5 cut(s) | 34, 342, 836, 930, 1224 |
| MluNI | TGGCCA | 1 cut(s) | 1153 |
| Mly113I | GGCGCC | 2 cut(s) | 233, 870 |
| MlyI | GAGTC | 1 cut(s) | 872 |
| MmeI | TCCRAC | 2 cut(s) | 63, 1244 |
| Mox20I | TGGCCA | 1 cut(s) | 1153 |
| MreI | CGCCGGCG | 1 cut(s) | 797 |
| MroNI | GCCGGC | 1 cut(s) | 797 |
| MscI | TGGCCA | 1 cut(s) | 1153 |
| MseI | TTAA | 2 cut(s) | 188, 224 |
| MslI | CAYNNNNRTG | 2 cut(s) | 380, 608 |
| Msp20I | TGGCCA | 1 cut(s) | 1153 |
| MspA1I | CMGCKG | 5 cut(s) | 208, 287, 775, 926, 1145 |
| MspI | CCGG | 7 cut(s) | 177, 320, 330, 362, 573, 798, 856 |
| MspR9I | CCNGG | 8 cut(s) | 71, 320, 330, 363, 573, 857, 912, 1263 |
| MunI | CAATTG | 1 cut(s) | 836 |
| MvaI | CCWGG | 3 cut(s) | 71, 912, 1263 |
| MvnI | CGCG | 1 cut(s) | 120 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 75, 300, 363, 530, 794, 803, 1180 |
| NaeI | GCCGGC | 1 cut(s) | 799 |
| NarI | GGCGCC | 2 cut(s) | 233, 870 |
| NciI | CCSGG | 5 cut(s) | 320, 330, 363, 573, 857 |
| NdeII | GATC | 9 cut(s) | 95, 106, 166, 179, 370, 403, 852, 997, 1265 |
| NgoMIV | GCCGGC | 1 cut(s) | 797 |
| NlaIII | CATG | 3 cut(s) | 1015, 1101, 1124 |
| NlaIV | GGNNCC | 6 cut(s) | 234, 591, 627, 651, 871, 916 |
| NmuCI | GTSAC | 3 cut(s) | 483, 546, 1030 |
| NspI | RCATGY | 1 cut(s) | 1101 |
| PdiI | GCCGGC | 1 cut(s) | 799 |
| PfeI | GAWTC | 2 cut(s) | 692, 889 |
| PflFI | GACNNNGTC | 1 cut(s) | 1122 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 820 |
| PkrI | GCNGC | 6 cut(s) | 124, 353, 356, 702, 928, 1182 |
| PleI | GAGTC | 1 cut(s) | 871 |
| PluTI | GGCGCC | 2 cut(s) | 236, 873 |
| PmaCI | CACGTG | 1 cut(s) | 1171 |
| PmlI | CACGTG | 1 cut(s) | 1171 |
| PpsI | GAGTC | 1 cut(s) | 871 |
| Ppu21I | YACGTR | 2 cut(s) | 551, 1171 |
| Psp1406I | AACGTT | 1 cut(s) | 221 |
| Psp6I | CCWGG | 3 cut(s) | 69, 910, 1261 |
| PspCI | CACGTG | 1 cut(s) | 1171 |
| PspFI | CCCAGC | 3 cut(s) | 283, 511, 874 |
| PspGI | CCWGG | 3 cut(s) | 69, 910, 1261 |
| PspN4I | GGNNCC | 6 cut(s) | 234, 591, 627, 651, 871, 916 |
| PspOMI | GGGCCC | 1 cut(s) | 914 |
| PspPI | GGNCC | 9 cut(s) | 4, 130, 364, 457, 650, 769, 914, 915, 1211 |
| PstI | CTGCAG | 1 cut(s) | 1182 |
| PstNI | CAGNNNCTG | 1 cut(s) | 850 |
| PsuI | RGATCY | 3 cut(s) | 370, 403, 1265 |
| PsyI | GACNNNGTC | 1 cut(s) | 1122 |
| PvuII | CAGCTG | 1 cut(s) | 287 |
| RsaI | GTAC | 5 cut(s) | 19, 31, 63, 386, 591 |
| RsaNI | GTAC | 5 cut(s) | 18, 30, 62, 385, 590 |
| RseI | CAYNNNNRTG | 2 cut(s) | 380, 608 |
| Rsr2I | CGGWCCG | 1 cut(s) | 130 |
| RsrII | CGGWCCG | 1 cut(s) | 130 |
| SalI | GTCGAC | 1 cut(s) | 567 |
| SaqAI | TTAA | 2 cut(s) | 188, 224 |
| SatI | GCNGC | 6 cut(s) | 123, 352, 355, 701, 927, 1181 |
| Sau3AI | GATC | 9 cut(s) | 95, 106, 166, 179, 370, 403, 852, 997, 1265 |
| Sau96I | GGNCC | 9 cut(s) | 4, 130, 364, 457, 650, 769, 914, 915, 1211 |
| SchI | GAGTC | 1 cut(s) | 872 |
| ScrFI | CCNGG | 8 cut(s) | 71, 320, 330, 363, 573, 857, 912, 1263 |
| SduI | GDGCHC | 1 cut(s) | 918 |
| SfaNI | GCATC | 5 cut(s) | 99, 600, 819, 1183, 1192 |
| SfcI | CTRYAG | 2 cut(s) | 288, 1178 |
| SfoI | GGCGCC | 2 cut(s) | 234, 871 |
| SgrAI | CRCCGGYG | 1 cut(s) | 797 |
| SinI | GGWCC | 4 cut(s) | 130, 650, 769, 1211 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 380, 608 |
| SmlI | CTYRAG | 2 cut(s) | 157, 1254 |
| SmoI | CTYRAG | 2 cut(s) | 157, 1254 |
| Sse9I | AATT | 5 cut(s) | 34, 342, 836, 930, 1224 |
| SspDI | GGCGCC | 2 cut(s) | 232, 869 |
| SspMI | CTAG | 4 cut(s) | 558, 677, 723, 1109 |
| StyD4I | CCNGG | 8 cut(s) | 69, 318, 328, 361, 571, 855, 910, 1261 |
| StyI | CCWWGG | 1 cut(s) | 755 |
| TaaI | ACNGT | 3 cut(s) | 134, 468, 546 |
| TaiI | ACGT | 5 cut(s) | 224, 490, 553, 1141, 1173 |
| TaqI | TCGA | 3 cut(s) | 165, 336, 568 |
| TasI | AATT | 5 cut(s) | 34, 342, 836, 930, 1224 |
| TauI | GCSGC | 3 cut(s) | 125, 357, 703 |
| TfiI | GAWTC | 2 cut(s) | 692, 889 |
| Tru1I | TTAA | 2 cut(s) | 188, 224 |
| Tru9I | TTAA | 2 cut(s) | 188, 224 |
| TscAI | CASTG | 3 cut(s) | 402, 627, 702 |
| TseFI | GTSAC | 3 cut(s) | 483, 546, 1030 |
| TseI | GCWGC | 3 cut(s) | 351, 926, 1180 |
| Tsp45I | GTSAC | 3 cut(s) | 483, 546, 1030 |
| TspDTI | ATGAA | 2 cut(s) | 398, 510 |
| TspGWI | ACGGA | 1 cut(s) | 143 |
| TspRI | CASTG | 3 cut(s) | 402, 627, 702 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1122 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 820 |
| VpaK11BI | GGWCC | 4 cut(s) | 130, 650, 769, 1211 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1200 |
| XceI | RCATGY | 1 cut(s) | 1101 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 817 |
| XmiI | GTMKAC | 1 cut(s) | 568 |
| XspI | CTAG | 4 cut(s) | 558, 677, 723, 1109 |