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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 8 cut(s) | 120, 130, 144, 156, 183, 208, 231, 254 |
| AclWI | GGATC | 1 cut(s) | 231 |
| AcoI | YGGCCR | 2 cut(s) | 3, 30 |
| AfaI | GTAC | 2 cut(s) | 64, 239 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 43, 214, 265 |
| AgsI | TTSAA | 2 cut(s) | 74, 163 |
| AhlI | ACTAGT | 1 cut(s) | 65 |
| AjnI | CCWGG | 1 cut(s) | 219 |
| AluBI | AGCT | 1 cut(s) | 244 |
| AluI | AGCT | 1 cut(s) | 244 |
| AlwI | GGATC | 1 cut(s) | 231 |
| AoxI | GGCC | 4 cut(s) | 3, 30, 217, 309 |
| ApeKI | GCWGC | 2 cut(s) | 201, 204 |
| AspS9I | GGNCC | 4 cut(s) | 122, 217, 256, 310 |
| AsuC2I | CCSGG | 2 cut(s) | 180, 216 |
| AsuHPI | GGTGA | 1 cut(s) | 197 |
| AvaII | GGWCC | 2 cut(s) | 122, 256 |
| BalI | TGGCCA | 1 cut(s) | 32 |
| BbvCI | CCTCAGC | 1 cut(s) | 306 |
| BbvI | GCAGC | 2 cut(s) | 213, 216 |
| BccI | CCATC | 2 cut(s) | 141, 156 |
| BciT130I | CCWGG | 1 cut(s) | 221 |
| BcnI | CCSGG | 2 cut(s) | 180, 216 |
| BcuI | ACTAGT | 1 cut(s) | 65 |
| BfaI | CTAG | 1 cut(s) | 66 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 216 |
| BisI | GCNGC | 3 cut(s) | 202, 205, 208 |
| BlsI | GCNGC | 3 cut(s) | 203, 206, 209 |
| BmcAI | AGTACT | 1 cut(s) | 64 |
| Bme1390I | CCNGG | 3 cut(s) | 180, 216, 221 |
| Bme18I | GGWCC | 2 cut(s) | 122, 256 |
| BmgT120I | GGNCC | 4 cut(s) | 122, 217, 256, 310 |
| BmrFI | CCNGG | 3 cut(s) | 180, 216, 221 |
| Bpu10I | CCTNAGC | 1 cut(s) | 306 |
| BpuMI | CCSGG | 2 cut(s) | 180, 216 |
| BsaJI | CCNNGG | 1 cut(s) | 178 |
| BsaWI | WCCGGW | 1 cut(s) | 43 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 43, 214, 265 |
| Bse1I | ACTGG | 2 cut(s) | 125, 255 |
| BseBI | CCWGG | 1 cut(s) | 221 |
| BseDI | CCNNGG | 1 cut(s) | 178 |
| BseGI | GGATG | 2 cut(s) | 133, 148 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 43, 214, 265 |
| BseMII | CTCAG | 1 cut(s) | 297 |
| BseNI | ACTGG | 2 cut(s) | 125, 255 |
| BseRI | GAGGAG | 1 cut(s) | 302 |
| BseXI | GCAGC | 2 cut(s) | 213, 216 |
| BseYI | CCCAGC | 2 cut(s) | 152, 169 |
| BshFI | GGCC | 4 cut(s) | 5, 32, 219, 311 |
| BsiSI | CCGG | 4 cut(s) | 6, 44, 180, 215 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 43, 214, 265 |
| BsnI | GGCC | 4 cut(s) | 5, 32, 219, 311 |
| Bsp143I | GATC | 1 cut(s) | 223 |
| BspACI | CCGC | 8 cut(s) | 120, 130, 144, 156, 183, 208, 231, 254 |
| BspANI | GGCC | 4 cut(s) | 5, 32, 219, 311 |
| BspCNI | CTCAG | 1 cut(s) | 298 |
| BspPI | GGATC | 1 cut(s) | 231 |
| BsrI | ACTGG | 2 cut(s) | 125, 255 |
| BssECI | CCNNGG | 1 cut(s) | 178 |
| BssMI | GATC | 1 cut(s) | 223 |
| Bst2UI | CCWGG | 1 cut(s) | 221 |
| Bst6I | CTCTTC | 3 cut(s) | 69, 105, 279 |
| BstC8I | GCNNGC | 1 cut(s) | 246 |
| BstDEI | CTNAG | 1 cut(s) | 306 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 2 cut(s) | 133, 148 |
| BstKTI | GATC | 1 cut(s) | 226 |
| BstMBI | GATC | 1 cut(s) | 223 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 207, 216 |
| BstNI | CCWGG | 1 cut(s) | 221 |
| BstSCI | CCNGG | 3 cut(s) | 178, 214, 219 |
| BstV1I | GCAGC | 2 cut(s) | 213, 216 |
| BstX2I | RGATCY | 1 cut(s) | 223 |
| BstYI | RGATCY | 1 cut(s) | 223 |
| BsuRI | GGCC | 4 cut(s) | 5, 32, 219, 311 |
| BtsCI | GGATG | 2 cut(s) | 133, 148 |
| BtsIMutI | CAGTG | 1 cut(s) | 248 |
| Cac8I | GCNNGC | 1 cut(s) | 246 |
| Cfr13I | GGNCC | 4 cut(s) | 122, 217, 256, 310 |
| Csp6I | GTAC | 2 cut(s) | 63, 238 |
| CviJI | RGCY | 8 cut(s) | 5, 32, 173, 207, 219, 244, 305, 311 |
| CviKI_1 | RGCY | 8 cut(s) | 5, 32, 173, 207, 219, 244, 305, 311 |
| CviQI | GTAC | 2 cut(s) | 63, 238 |
| DdeI | CTNAG | 1 cut(s) | 306 |
| DpnI | GATC | 1 cut(s) | 225 |
| DpnII | GATC | 1 cut(s) | 223 |
| EaeI | YGGCCR | 2 cut(s) | 3, 30 |
| Eam1104I | CTCTTC | 3 cut(s) | 69, 105, 279 |
| EarI | CTCTTC | 3 cut(s) | 69, 105, 279 |
| EciI | GGCGGA | 2 cut(s) | 119, 269 |
| Eco47I | GGWCC | 2 cut(s) | 122, 256 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoRII | CCWGG | 1 cut(s) | 219 |
| FaiI | YATR | 1 cut(s) | 236 |
| Fnu4HI | GCNGC | 3 cut(s) | 202, 205, 208 |
| FokI | GGATG | 2 cut(s) | 120, 135 |
| Fsp4HI | GCNGC | 3 cut(s) | 202, 205, 208 |
| FspBI | CTAG | 1 cut(s) | 66 |
| GluI | GCNGC | 3 cut(s) | 202, 205, 208 |
| GsaI | CCCAGC | 2 cut(s) | 156, 173 |
| HaeIII | GGCC | 4 cut(s) | 5, 32, 219, 311 |
| HapII | CCGG | 4 cut(s) | 6, 44, 180, 215 |
| HinfI | GANTC | 2 cut(s) | 40, 80 |
| HpaII | CCGG | 4 cut(s) | 6, 44, 180, 215 |
| HphI | GGTGA | 1 cut(s) | 197 |
| Hpy188I | TCNGA | 1 cut(s) | 85 |
| HpyAV | CCTTC | 3 cut(s) | 89, 269, 295 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 207, 216 |
| HpyF3I | CTNAG | 1 cut(s) | 306 |
| Kzo9I | GATC | 1 cut(s) | 223 |
| Lsp1109I | GCAGC | 2 cut(s) | 213, 216 |
| MaeI | CTAG | 1 cut(s) | 66 |
| MalI | GATC | 1 cut(s) | 225 |
| MboI | GATC | 1 cut(s) | 223 |
| MboII | GAAGA | 4 cut(s) | 86, 100, 122, 296 |
| MflI | RGATCY | 1 cut(s) | 223 |
| MlsI | TGGCCA | 1 cut(s) | 32 |
| MluNI | TGGCCA | 1 cut(s) | 32 |
| MnlI | CCTC | 4 cut(s) | 70, 181, 280, 301 |
| Mox20I | TGGCCA | 1 cut(s) | 32 |
| MscI | TGGCCA | 1 cut(s) | 32 |
| Msp20I | TGGCCA | 1 cut(s) | 32 |
| MspA1I | CMGCKG | 1 cut(s) | 156 |
| MspI | CCGG | 4 cut(s) | 6, 44, 180, 215 |
| MspR9I | CCNGG | 3 cut(s) | 180, 216, 221 |
| MvaI | CCWGG | 1 cut(s) | 221 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 207, 216 |
| NciI | CCSGG | 2 cut(s) | 180, 216 |
| NdeII | GATC | 1 cut(s) | 223 |
| PfeI | GAWTC | 2 cut(s) | 40, 80 |
| PkrI | GCNGC | 3 cut(s) | 203, 206, 209 |
| Psp6I | CCWGG | 1 cut(s) | 219 |
| PspFI | CCCAGC | 2 cut(s) | 152, 169 |
| PspGI | CCWGG | 1 cut(s) | 219 |
| PspPI | GGNCC | 4 cut(s) | 122, 217, 256, 310 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 8, 40 |
| PsuI | RGATCY | 1 cut(s) | 223 |
| RsaI | GTAC | 2 cut(s) | 64, 239 |
| RsaNI | GTAC | 2 cut(s) | 63, 238 |
| SatI | GCNGC | 3 cut(s) | 202, 205, 208 |
| Sau3AI | GATC | 1 cut(s) | 223 |
| Sau96I | GGNCC | 4 cut(s) | 122, 217, 256, 310 |
| ScaI | AGTACT | 1 cut(s) | 64 |
| ScrFI | CCNGG | 3 cut(s) | 180, 216, 221 |
| SetI | ASST | 2 cut(s) | 246, 261 |
| SinI | GGWCC | 2 cut(s) | 122, 256 |
| SpeI | ACTAGT | 1 cut(s) | 65 |
| SsiI | CCGC | 8 cut(s) | 120, 130, 144, 156, 183, 208, 231, 254 |
| SspMI | CTAG | 1 cut(s) | 66 |
| StyD4I | CCNGG | 3 cut(s) | 178, 214, 219 |
| TatI | WGTACW | 1 cut(s) | 62 |
| TauI | GCSGC | 1 cut(s) | 210 |
| TfiI | GAWTC | 2 cut(s) | 40, 80 |
| TscAI | CASTG | 1 cut(s) | 255 |
| TseI | GCWGC | 2 cut(s) | 201, 204 |
| TspRI | CASTG | 1 cut(s) | 255 |
| VpaK11BI | GGWCC | 2 cut(s) | 122, 256 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XspI | CTAG | 1 cut(s) | 66 |
| ZrmI | AGTACT | 1 cut(s) | 64 |