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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 292 |
| Acc36I | ACCTGC | 1 cut(s) | 292 |
| AccII | CGCG | 2 cut(s) | 74, 264 |
| AciI | CCGC | 4 cut(s) | 38, 72, 74, 149 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AclWI | GGATC | 2 cut(s) | 48, 213 |
| AcoI | YGGCCR | 1 cut(s) | 306 |
| AcsI | RAATTY | 1 cut(s) | 323 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 121, 247, 301 |
| AgsI | TTSAA | 2 cut(s) | 100, 328 |
| AjnI | CCWGG | 1 cut(s) | 141 |
| AluBI | AGCT | 1 cut(s) | 334 |
| AluI | AGCT | 1 cut(s) | 334 |
| AlwI | GGATC | 2 cut(s) | 48, 213 |
| AoxI | GGCC | 3 cut(s) | 199, 291, 306 |
| ApeKI | GCWGC | 1 cut(s) | 183 |
| ApoI | RAATTY | 1 cut(s) | 323 |
| AspLEI | GCGC | 3 cut(s) | 264, 276, 345 |
| AspS9I | GGNCC | 2 cut(s) | 145, 292 |
| AsuC2I | CCSGG | 1 cut(s) | 203 |
| AvaII | GGWCC | 1 cut(s) | 145 |
| BalI | TGGCCA | 1 cut(s) | 308 |
| BbsI | GAAGAC | 1 cut(s) | 290 |
| BbvI | GCAGC | 1 cut(s) | 195 |
| BccI | CCATC | 2 cut(s) | 66, 202 |
| BciT130I | CCWGG | 1 cut(s) | 143 |
| BcnI | CCSGG | 1 cut(s) | 203 |
| BfaI | CTAG | 2 cut(s) | 117, 364 |
| BfmI | CTRYAG | 1 cut(s) | 10 |
| BfoI | RGCGCY | 1 cut(s) | 346 |
| BfuAI | ACCTGC | 1 cut(s) | 292 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 198 |
| BisI | GCNGC | 1 cut(s) | 184 |
| BlsI | GCNGC | 1 cut(s) | 185 |
| Bme1390I | CCNGG | 2 cut(s) | 143, 203 |
| Bme18I | GGWCC | 1 cut(s) | 145 |
| BmgT120I | GGNCC | 2 cut(s) | 145, 292 |
| BmiI | GGNNCC | 2 cut(s) | 126, 147 |
| BmrFI | CCNGG | 2 cut(s) | 143, 203 |
| BpiI | GAAGAC | 1 cut(s) | 290 |
| BpmI | CTGGAG | 2 cut(s) | 90, 188 |
| BpuMI | CCSGG | 1 cut(s) | 203 |
| BsaJI | CCNNGG | 4 cut(s) | 72, 130, 141, 288 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 121, 247, 301 |
| Bse1I | ACTGG | 2 cut(s) | 107, 171 |
| BseBI | CCWGG | 1 cut(s) | 143 |
| BseDI | CCNNGG | 4 cut(s) | 72, 130, 141, 288 |
| BseGI | GGATG | 2 cut(s) | 58, 137 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 121, 247, 301 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseNI | ACTGG | 2 cut(s) | 107, 171 |
| BseXI | GCAGC | 1 cut(s) | 195 |
| BsgI | GTGCAG | 1 cut(s) | 147 |
| Bsh1236I | CGCG | 2 cut(s) | 74, 264 |
| BshFI | GGCC | 3 cut(s) | 201, 293, 308 |
| BsiSI | CCGG | 1 cut(s) | 202 |
| BslFI | GGGAC | 1 cut(s) | 131 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 121, 247, 301 |
| BsmFI | GGGAC | 1 cut(s) | 131 |
| BsnI | GGCC | 3 cut(s) | 201, 293, 308 |
| Bsp143I | GATC | 2 cut(s) | 40, 205 |
| BspACI | CCGC | 4 cut(s) | 38, 72, 74, 149 |
| BspANI | GGCC | 3 cut(s) | 201, 293, 308 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspFNI | CGCG | 2 cut(s) | 74, 264 |
| BspLI | GGNNCC | 2 cut(s) | 126, 147 |
| BspMI | ACCTGC | 1 cut(s) | 292 |
| BspPI | GGATC | 2 cut(s) | 48, 213 |
| BspQI | GCTCTTC | 1 cut(s) | 264 |
| BsrI | ACTGG | 2 cut(s) | 107, 171 |
| BssECI | CCNNGG | 4 cut(s) | 72, 130, 141, 288 |
| BssMI | GATC | 2 cut(s) | 40, 205 |
| BssT1I | CCWWGG | 1 cut(s) | 130 |
| Bst2UI | CCWGG | 1 cut(s) | 143 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 1 cut(s) | 264 |
| BstC8I | GCNNGC | 1 cut(s) | 36 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstDSI | CCRYGG | 1 cut(s) | 72 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 245 |
| BstF5I | GGATG | 2 cut(s) | 58, 137 |
| BstFNI | CGCG | 2 cut(s) | 74, 264 |
| BstH2I | RGCGCY | 1 cut(s) | 346 |
| BstHHI | GCGC | 3 cut(s) | 264, 276, 345 |
| BstKTI | GATC | 2 cut(s) | 43, 208 |
| BstMBI | GATC | 2 cut(s) | 40, 205 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 170, 189, 198, 340, 349 |
| BstNI | CCWGG | 1 cut(s) | 143 |
| BstSCI | CCNGG | 2 cut(s) | 141, 201 |
| BstSFI | CTRYAG | 1 cut(s) | 10 |
| BstUI | CGCG | 2 cut(s) | 74, 264 |
| BstV1I | GCAGC | 1 cut(s) | 195 |
| BstV2I | GAAGAC | 1 cut(s) | 290 |
| BstX2I | RGATCY | 1 cut(s) | 205 |
| BstYI | RGATCY | 1 cut(s) | 205 |
| BsuRI | GGCC | 3 cut(s) | 201, 293, 308 |
| BtgI | CCRYGG | 1 cut(s) | 72 |
| BtsCI | GGATG | 2 cut(s) | 58, 137 |
| BtsIMutI | CAGTG | 1 cut(s) | 164 |
| BveI | ACCTGC | 1 cut(s) | 292 |
| Cac8I | GCNNGC | 1 cut(s) | 36 |
| CfoI | GCGC | 3 cut(s) | 264, 276, 345 |
| Cfr13I | GGNCC | 2 cut(s) | 145, 292 |
| Cfr42I | CCGCGG | 1 cut(s) | 75 |
| CviAII | CATG | 1 cut(s) | 121 |
| CviJI | RGCY | 5 cut(s) | 135, 201, 293, 308, 334 |
| CviKI_1 | RGCY | 5 cut(s) | 135, 201, 293, 308, 334 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 2 cut(s) | 42, 207 |
| DpnII | GATC | 2 cut(s) | 40, 205 |
| EaeI | YGGCCR | 1 cut(s) | 306 |
| Eam1104I | CTCTTC | 1 cut(s) | 264 |
| EarI | CTCTTC | 1 cut(s) | 264 |
| Eco130I | CCWWGG | 1 cut(s) | 130 |
| Eco47I | GGWCC | 1 cut(s) | 145 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 245 |
| EcoO109I | RGGNCCY | 1 cut(s) | 145 |
| EcoRII | CCWGG | 1 cut(s) | 141 |
| EcoT14I | CCWWGG | 1 cut(s) | 130 |
| ErhI | CCWWGG | 1 cut(s) | 130 |
| FaeI | CATG | 1 cut(s) | 124 |
| FaiI | YATR | 3 cut(s) | 122, 222, 354 |
| FaqI | GGGAC | 1 cut(s) | 131 |
| FatI | CATG | 1 cut(s) | 120 |
| FauI | CCCGC | 2 cut(s) | 79, 156 |
| Fnu4HI | GCNGC | 1 cut(s) | 184 |
| FokI | GGATG | 2 cut(s) | 45, 124 |
| Fsp4HI | GCNGC | 1 cut(s) | 184 |
| FspBI | CTAG | 2 cut(s) | 117, 364 |
| GlaI | GCGC | 3 cut(s) | 263, 275, 344 |
| GluI | GCNGC | 1 cut(s) | 184 |
| GsuI | CTGGAG | 2 cut(s) | 90, 188 |
| HaeII | RGCGCY | 1 cut(s) | 346 |
| HaeIII | GGCC | 3 cut(s) | 201, 293, 308 |
| HapII | CCGG | 1 cut(s) | 202 |
| HhaI | GCGC | 3 cut(s) | 264, 276, 345 |
| Hin1II | CATG | 1 cut(s) | 124 |
| Hin6I | GCGC | 3 cut(s) | 262, 274, 343 |
| HinP1I | GCGC | 3 cut(s) | 262, 274, 343 |
| HincII | GTYRAC | 4 cut(s) | 79, 85, 112, 228 |
| HindII | GTYRAC | 4 cut(s) | 79, 85, 112, 228 |
| HpaI | GTTAAC | 3 cut(s) | 79, 85, 112 |
| HpaII | CCGG | 1 cut(s) | 202 |
| Hpy166II | GTNNAC | 4 cut(s) | 79, 85, 112, 228 |
| Hpy8I | GTNNAC | 4 cut(s) | 79, 85, 112, 228 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 1 cut(s) | 81 |
| HpyCH4V | TGCA | 1 cut(s) | 164 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 170, 189, 198, 340, 349 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| HpySE526I | ACGT | 1 cut(s) | 81 |
| Hsp92II | CATG | 1 cut(s) | 124 |
| HspAI | GCGC | 3 cut(s) | 262, 274, 343 |
| KspAI | GTTAAC | 3 cut(s) | 79, 85, 112 |
| KspI | CCGCGG | 1 cut(s) | 75 |
| Kzo9I | GATC | 2 cut(s) | 40, 205 |
| LguI | GCTCTTC | 1 cut(s) | 264 |
| LmnI | GCTCC | 1 cut(s) | 259 |
| LpnPI | CCDG | 9 cut(s) | 120, 128, 152, 155, 170, 183, 215, 287, 331 |
| Lsp1109I | GCAGC | 1 cut(s) | 195 |
| MaeI | CTAG | 2 cut(s) | 117, 364 |
| MaeII | ACGT | 1 cut(s) | 81 |
| MalI | GATC | 2 cut(s) | 42, 207 |
| MboI | GATC | 2 cut(s) | 40, 205 |
| MboII | GAAGA | 3 cut(s) | 278, 281, 295 |
| MflI | RGATCY | 1 cut(s) | 205 |
| MlsI | TGGCCA | 1 cut(s) | 308 |
| MluCI | AATT | 1 cut(s) | 323 |
| MluNI | TGGCCA | 1 cut(s) | 308 |
| MnlI | CCTC | 5 cut(s) | 10, 163, 251, 283, 342 |
| Mox20I | TGGCCA | 1 cut(s) | 308 |
| MscI | TGGCCA | 1 cut(s) | 308 |
| MseI | TTAA | 3 cut(s) | 78, 84, 111 |
| MslI | CAYNNNNRTG | 1 cut(s) | 314 |
| Msp20I | TGGCCA | 1 cut(s) | 308 |
| MspA1I | CMGCKG | 2 cut(s) | 74, 151 |
| MspI | CCGG | 1 cut(s) | 202 |
| MspR9I | CCNGG | 2 cut(s) | 143, 203 |
| MvaI | CCWGG | 1 cut(s) | 143 |
| MvnI | CGCG | 2 cut(s) | 74, 264 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 170, 189, 198, 340, 349 |
| NciI | CCSGG | 1 cut(s) | 203 |
| NdeII | GATC | 2 cut(s) | 40, 205 |
| NlaIII | CATG | 1 cut(s) | 124 |
| NlaIV | GGNNCC | 2 cut(s) | 126, 147 |
| PaqCI | CACCTGC | 1 cut(s) | 292 |
| PciSI | GCTCTTC | 1 cut(s) | 264 |
| PkrI | GCNGC | 1 cut(s) | 185 |
| PpuMI | RGGWCCY | 1 cut(s) | 145 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| Psp5II | RGGWCCY | 1 cut(s) | 145 |
| Psp6I | CCWGG | 1 cut(s) | 141 |
| PspGI | CCWGG | 1 cut(s) | 141 |
| PspN4I | GGNNCC | 2 cut(s) | 126, 147 |
| PspPI | GGNCC | 2 cut(s) | 145, 292 |
| PspPPI | RGGWCCY | 1 cut(s) | 145 |
| PsuI | RGATCY | 1 cut(s) | 205 |
| RseI | CAYNNNNRTG | 1 cut(s) | 314 |
| SacII | CCGCGG | 1 cut(s) | 75 |
| SapI | GCTCTTC | 1 cut(s) | 264 |
| SaqAI | TTAA | 3 cut(s) | 78, 84, 111 |
| SatI | GCNGC | 1 cut(s) | 184 |
| Sau3AI | GATC | 2 cut(s) | 40, 205 |
| Sau96I | GGNCC | 2 cut(s) | 145, 292 |
| ScrFI | CCNGG | 2 cut(s) | 143, 203 |
| SetI | ASST | 7 cut(s) | 84, 147, 221, 243, 306, 336, 365 |
| SfcI | CTRYAG | 1 cut(s) | 10 |
| Sfr303I | CCGCGG | 1 cut(s) | 75 |
| SgrBI | CCGCGG | 1 cut(s) | 75 |
| SinI | GGWCC | 1 cut(s) | 145 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 314 |
| Sse9I | AATT | 1 cut(s) | 323 |
| SsiI | CCGC | 4 cut(s) | 38, 72, 74, 149 |
| SspMI | CTAG | 2 cut(s) | 117, 364 |
| StyD4I | CCNGG | 2 cut(s) | 141, 201 |
| StyI | CCWWGG | 1 cut(s) | 130 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 1 cut(s) | 84 |
| TaqII | GACCGA | 1 cut(s) | 302 |
| TasI | AATT | 1 cut(s) | 323 |
| Tru1I | TTAA | 3 cut(s) | 78, 84, 111 |
| Tru9I | TTAA | 3 cut(s) | 78, 84, 111 |
| TscAI | CASTG | 1 cut(s) | 171 |
| TseI | GCWGC | 1 cut(s) | 183 |
| TspRI | CASTG | 1 cut(s) | 171 |
| VpaK11BI | GGWCC | 1 cut(s) | 145 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 245 |
| XapI | RAATTY | 1 cut(s) | 323 |
| XspI | CTAG | 2 cut(s) | 117, 364 |