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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 2 cut(s) | 498, 608 |
| AccII | CGCG | 2 cut(s) | 213, 334 |
| AciI | CCGC | 9 cut(s) | 38, 48, 125, 139, 334, 366, 485, 496, 608 |
| AclI | AACGTT | 2 cut(s) | 81, 99 |
| AclWI | GGATC | 1 cut(s) | 48 |
| AcsI | RAATTY | 1 cut(s) | 272 |
| AfaI | GTAC | 4 cut(s) | 121, 170, 375, 402 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 131, 145, 196, 250, 362, 638 |
| AgsI | TTSAA | 1 cut(s) | 409 |
| AluBI | AGCT | 4 cut(s) | 175, 317, 421, 548 |
| AluI | AGCT | 4 cut(s) | 175, 317, 421, 548 |
| Alw21I | GWGCWC | 2 cut(s) | 112, 341 |
| Alw26I | GTCTC | 1 cut(s) | 577 |
| Alw44I | GTGCAC | 1 cut(s) | 108 |
| AlwI | GGATC | 1 cut(s) | 48 |
| AoxI | GGCC | 3 cut(s) | 234, 240, 510 |
| ApaLI | GTGCAC | 1 cut(s) | 108 |
| ApeKI | GCWGC | 2 cut(s) | 135, 463 |
| ApoI | RAATTY | 1 cut(s) | 272 |
| AspA2I | CCTAGG | 1 cut(s) | 539 |
| AspLEI | GCGC | 3 cut(s) | 213, 295, 348 |
| AspS9I | GGNCC | 4 cut(s) | 241, 434, 510, 573 |
| AsuC2I | CCSGG | 2 cut(s) | 146, 639 |
| AsuHPI | GGTGA | 1 cut(s) | 352 |
| AvaII | GGWCC | 2 cut(s) | 434, 573 |
| AvrII | CCTAGG | 1 cut(s) | 539 |
| BaeGI | GKGCMC | 1 cut(s) | 112 |
| Bbv12I | GWGCWC | 2 cut(s) | 112, 341 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 2 cut(s) | 147, 475 |
| BccI | CCATC | 2 cut(s) | 365, 573 |
| BcnI | CCSGG | 2 cut(s) | 146, 639 |
| BcoDI | GTCTC | 1 cut(s) | 577 |
| BfaI | CTAG | 3 cut(s) | 182, 342, 540 |
| BfoI | RGCGCY | 1 cut(s) | 296 |
| BisI | GCNGC | 5 cut(s) | 136, 139, 464, 485, 496 |
| BlnI | CCTAGG | 1 cut(s) | 539 |
| BlsI | GCNGC | 5 cut(s) | 137, 140, 465, 486, 497 |
| Bme1390I | CCNGG | 2 cut(s) | 146, 639 |
| Bme18I | GGWCC | 2 cut(s) | 434, 573 |
| BmgT120I | GGNCC | 4 cut(s) | 241, 434, 510, 573 |
| BmiI | GGNNCC | 2 cut(s) | 574, 575 |
| BmrFI | CCNGG | 2 cut(s) | 146, 639 |
| BmsI | GCATC | 2 cut(s) | 571, 601 |
| BpmI | CTGGAG | 1 cut(s) | 624 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 2 cut(s) | 146, 639 |
| BsaJI | CCNNGG | 4 cut(s) | 144, 237, 519, 539 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 461, 491 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 131, 145, 196, 250, 362, 638 |
| Bse118I | RCCGGY | 1 cut(s) | 362 |
| Bse1I | ACTGG | 1 cut(s) | 607 |
| BseDI | CCNNGG | 4 cut(s) | 144, 237, 519, 539 |
| BseGI | GGATG | 2 cut(s) | 562, 616 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 131, 145, 196, 250, 362, 638 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseNI | ACTGG | 1 cut(s) | 607 |
| BseSI | GKGCMC | 1 cut(s) | 112 |
| BseXI | GCAGC | 2 cut(s) | 147, 475 |
| BseYI | CCCAGC | 1 cut(s) | 313 |
| Bsh1236I | CGCG | 2 cut(s) | 213, 334 |
| BshFI | GGCC | 3 cut(s) | 236, 242, 512 |
| BsiHKAI | GWGCWC | 2 cut(s) | 112, 341 |
| BsiSI | CCGG | 3 cut(s) | 146, 363, 638 |
| BslFI | GGGAC | 2 cut(s) | 521, 586 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 131, 145, 196, 250, 362, 638 |
| BsmAI | GTCTC | 1 cut(s) | 577 |
| BsmFI | GGGAC | 2 cut(s) | 521, 586 |
| BsnI | GGCC | 3 cut(s) | 236, 242, 512 |
| Bsp1286I | GDGCHC | 2 cut(s) | 112, 341 |
| Bsp143I | GATC | 2 cut(s) | 40, 75 |
| BspACI | CCGC | 9 cut(s) | 38, 48, 125, 139, 334, 366, 485, 496, 608 |
| BspANI | GGCC | 3 cut(s) | 236, 242, 512 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspFNI | CGCG | 2 cut(s) | 213, 334 |
| BspLI | GGNNCC | 2 cut(s) | 574, 575 |
| BspPI | GGATC | 1 cut(s) | 48 |
| BsrBI | CCGCTC | 2 cut(s) | 498, 608 |
| BsrFI | RCCGGY | 1 cut(s) | 362 |
| BsrI | ACTGG | 1 cut(s) | 607 |
| BssAI | RCCGGY | 1 cut(s) | 362 |
| BssECI | CCNNGG | 4 cut(s) | 144, 237, 519, 539 |
| BssMI | GATC | 2 cut(s) | 40, 75 |
| BssT1I | CCWWGG | 1 cut(s) | 539 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 405 |
| Bst6I | CTCTTC | 1 cut(s) | 294 |
| BstC8I | GCNNGC | 3 cut(s) | 177, 215, 546 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 194 |
| BstF5I | GGATG | 2 cut(s) | 562, 616 |
| BstFNI | CGCG | 2 cut(s) | 213, 334 |
| BstH2I | RGCGCY | 1 cut(s) | 296 |
| BstHHI | GCGC | 3 cut(s) | 213, 295, 348 |
| BstKTI | GATC | 2 cut(s) | 43, 78 |
| BstMAI | GTCTC | 1 cut(s) | 577 |
| BstMBI | GATC | 2 cut(s) | 40, 75 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 314, 345, 352, 554 |
| BstNSI | RCATGY | 1 cut(s) | 536 |
| BstSCI | CCNGG | 2 cut(s) | 144, 637 |
| BstSLI | GKGCMC | 1 cut(s) | 112 |
| BstUI | CGCG | 2 cut(s) | 213, 334 |
| BstV1I | GCAGC | 2 cut(s) | 147, 475 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 602 |
| BsuRI | GGCC | 3 cut(s) | 236, 242, 512 |
| BtsCI | GGATG | 2 cut(s) | 562, 616 |
| Cac8I | GCNNGC | 3 cut(s) | 177, 215, 546 |
| CfoI | GCGC | 3 cut(s) | 213, 295, 348 |
| Cfr10I | RCCGGY | 1 cut(s) | 362 |
| Cfr13I | GGNCC | 4 cut(s) | 241, 434, 510, 573 |
| Csp6I | GTAC | 4 cut(s) | 120, 169, 374, 401 |
| CviAII | CATG | 1 cut(s) | 533 |
| CviQI | GTAC | 4 cut(s) | 120, 169, 374, 401 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 2 cut(s) | 42, 77 |
| DpnII | GATC | 2 cut(s) | 40, 75 |
| Eam1104I | CTCTTC | 1 cut(s) | 294 |
| EarI | CTCTTC | 1 cut(s) | 294 |
| EciI | GGCGGA | 1 cut(s) | 381 |
| Eco130I | CCWWGG | 1 cut(s) | 539 |
| Eco47I | GGWCC | 2 cut(s) | 434, 573 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 194 |
| EcoO109I | RGGNCCY | 1 cut(s) | 573 |
| EcoT14I | CCWWGG | 1 cut(s) | 539 |
| EcoT22I | ATGCAT | 1 cut(s) | 534 |
| ErhI | CCWWGG | 1 cut(s) | 539 |
| FaeI | CATG | 1 cut(s) | 536 |
| FaiI | YATR | 8 cut(s) | 167, 389, 393, 395, 489, 530, 534, 597 |
| FaqI | GGGAC | 2 cut(s) | 521, 586 |
| FatI | CATG | 1 cut(s) | 532 |
| FauI | CCCGC | 1 cut(s) | 55 |
| FauNDI | CATATG | 1 cut(s) | 393 |
| Fnu4HI | GCNGC | 5 cut(s) | 136, 139, 464, 485, 496 |
| FokI | GGATG | 2 cut(s) | 549, 623 |
| Fsp4HI | GCNGC | 5 cut(s) | 136, 139, 464, 485, 496 |
| FspBI | CTAG | 3 cut(s) | 182, 342, 540 |
| GlaI | GCGC | 3 cut(s) | 212, 294, 347 |
| GluI | GCNGC | 5 cut(s) | 136, 139, 464, 485, 496 |
| GsaI | CCCAGC | 1 cut(s) | 317 |
| GsuI | CTGGAG | 1 cut(s) | 624 |
| HaeII | RGCGCY | 1 cut(s) | 296 |
| HaeIII | GGCC | 3 cut(s) | 236, 242, 512 |
| HapII | CCGG | 3 cut(s) | 146, 363, 638 |
| HhaI | GCGC | 3 cut(s) | 213, 295, 348 |
| Hin1II | CATG | 1 cut(s) | 536 |
| Hin6I | GCGC | 3 cut(s) | 211, 293, 346 |
| HinP1I | GCGC | 3 cut(s) | 211, 293, 346 |
| HinfI | GANTC | 1 cut(s) | 476 |
| HpaII | CCGG | 3 cut(s) | 146, 363, 638 |
| HphI | GGTGA | 1 cut(s) | 352 |
| Hpy166II | GTNNAC | 3 cut(s) | 110, 122, 376 |
| Hpy188III | TCNNGA | 2 cut(s) | 443, 566 |
| Hpy8I | GTNNAC | 3 cut(s) | 110, 122, 376 |
| HpyAV | CCTTC | 1 cut(s) | 283 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 405 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 99 |
| HpyCH4V | TGCA | 6 cut(s) | 110, 151, 397, 463, 532, 614 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 314, 345, 352, 554 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| HpySE526I | ACGT | 2 cut(s) | 81, 99 |
| Hsp92II | CATG | 1 cut(s) | 536 |
| HspAI | GCGC | 3 cut(s) | 211, 293, 346 |
| KflI | GGGWCCC | 1 cut(s) | 573 |
| Kzo9I | GATC | 2 cut(s) | 40, 75 |
| LmnI | GCTCC | 2 cut(s) | 336, 605 |
| Lsp1109I | GCAGC | 2 cut(s) | 147, 475 |
| LweI | GCATC | 2 cut(s) | 571, 601 |
| MaeI | CTAG | 3 cut(s) | 182, 342, 540 |
| MaeII | ACGT | 2 cut(s) | 81, 99 |
| MaeIII | GTNAC | 1 cut(s) | 477 |
| MalI | GATC | 2 cut(s) | 42, 77 |
| MbiI | CCGCTC | 2 cut(s) | 498, 608 |
| MboI | GATC | 2 cut(s) | 40, 75 |
| MboII | GAAGA | 1 cut(s) | 311 |
| MhlI | GDGCHC | 2 cut(s) | 112, 341 |
| MluCI | AATT | 2 cut(s) | 272, 618 |
| MlyI | GAGTC | 1 cut(s) | 485 |
| Mph1103I | ATGCAT | 1 cut(s) | 534 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| MslI | CAYNNNNRTG | 2 cut(s) | 263, 392 |
| MspI | CCGG | 3 cut(s) | 146, 363, 638 |
| MspR9I | CCNGG | 2 cut(s) | 146, 639 |
| MvnI | CGCG | 2 cut(s) | 213, 334 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 314, 345, 352, 554 |
| NciI | CCSGG | 2 cut(s) | 146, 639 |
| NdeI | CATATG | 1 cut(s) | 393 |
| NdeII | GATC | 2 cut(s) | 40, 75 |
| NlaIII | CATG | 1 cut(s) | 536 |
| NlaIV | GGNNCC | 2 cut(s) | 574, 575 |
| NmeAIII | GCCGAG | 2 cut(s) | 153, 262 |
| NmuCI | GTSAC | 1 cut(s) | 477 |
| NsiI | ATGCAT | 1 cut(s) | 534 |
| NspI | RCATGY | 1 cut(s) | 536 |
| PkrI | GCNGC | 5 cut(s) | 137, 140, 465, 486, 497 |
| PleI | GAGTC | 1 cut(s) | 484 |
| PpsI | GAGTC | 1 cut(s) | 484 |
| PpuMI | RGGWCCY | 1 cut(s) | 573 |
| Psp1406I | AACGTT | 2 cut(s) | 81, 99 |
| Psp5II | RGGWCCY | 1 cut(s) | 573 |
| PspFI | CCCAGC | 1 cut(s) | 313 |
| PspN4I | GGNNCC | 2 cut(s) | 574, 575 |
| PspPI | GGNCC | 4 cut(s) | 241, 434, 510, 573 |
| PspPPI | RGGWCCY | 1 cut(s) | 573 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 372, 404 |
| RsaI | GTAC | 4 cut(s) | 121, 170, 375, 402 |
| RsaNI | GTAC | 4 cut(s) | 120, 169, 374, 401 |
| RseI | CAYNNNNRTG | 2 cut(s) | 263, 392 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 5 cut(s) | 136, 139, 464, 485, 496 |
| Sau3AI | GATC | 2 cut(s) | 40, 75 |
| Sau96I | GGNCC | 4 cut(s) | 241, 434, 510, 573 |
| SchI | GAGTC | 1 cut(s) | 485 |
| ScrFI | CCNGG | 2 cut(s) | 146, 639 |
| SduI | GDGCHC | 2 cut(s) | 112, 341 |
| SfaNI | GCATC | 2 cut(s) | 571, 601 |
| SgrAI | CRCCGGYG | 1 cut(s) | 362 |
| SinI | GGWCC | 2 cut(s) | 434, 573 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 263, 392 |
| Sse9I | AATT | 2 cut(s) | 272, 618 |
| SsiI | CCGC | 9 cut(s) | 38, 48, 125, 139, 334, 366, 485, 496, 608 |
| SspMI | CTAG | 3 cut(s) | 182, 342, 540 |
| StyD4I | CCNGG | 2 cut(s) | 144, 637 |
| StyI | CCWWGG | 1 cut(s) | 539 |
| TaaI | ACNGT | 2 cut(s) | 11, 405 |
| TaiI | ACGT | 2 cut(s) | 84, 102 |
| TaqI | TCGA | 1 cut(s) | 500 |
| TasI | AATT | 2 cut(s) | 272, 618 |
| TatI | WGTACW | 1 cut(s) | 119 |
| TauI | GCSGC | 3 cut(s) | 141, 487, 498 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TseFI | GTSAC | 1 cut(s) | 477 |
| TseI | GCWGC | 2 cut(s) | 135, 463 |
| Tsp45I | GTSAC | 1 cut(s) | 477 |
| VneI | GTGCAC | 1 cut(s) | 108 |
| VpaK11BI | GGWCC | 2 cut(s) | 434, 573 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 194 |
| XapI | RAATTY | 1 cut(s) | 272 |
| XceI | RCATGY | 1 cut(s) | 536 |
| XmaJI | CCTAGG | 1 cut(s) | 539 |
| XspI | CTAG | 3 cut(s) | 182, 342, 540 |
| Zsp2I | ATGCAT | 1 cut(s) | 534 |