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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 409, 898 |
| Acc36I | ACCTGC | 5 cut(s) | 14, 153, 409, 621, 898 |
| AccB1I | GGYRCC | 4 cut(s) | 194, 662, 1164, 1172 |
| AccBSI | CCGCTC | 2 cut(s) | 1047, 1107 |
| AclWI | GGATC | 6 cut(s) | 168, 363, 636, 852, 1080, 1093 |
| AcoI | YGGCCR | 6 cut(s) | 18, 164, 423, 632, 912, 1234 |
| AcyI | GRCGYC | 2 cut(s) | 195, 663 |
| AfaI | GTAC | 1 cut(s) | 505 |
| AgsI | TTSAA | 1 cut(s) | 1183 |
| AjnI | CCWGG | 8 cut(s) | 20, 42, 155, 313, 623, 802, 1200, 1205 |
| AleI | CACNNNNGTG | 2 cut(s) | 431, 920 |
| AluBI | AGCT | 5 cut(s) | 97, 343, 565, 832, 1179 |
| AluI | AGCT | 5 cut(s) | 97, 343, 565, 832, 1179 |
| Alw26I | GTCTC | 3 cut(s) | 281, 770, 1007 |
| AlwI | GGATC | 6 cut(s) | 168, 363, 636, 852, 1080, 1093 |
| Ama87I | CYCGRG | 1 cut(s) | 1018 |
| ApeKI | GCWGC | 3 cut(s) | 303, 792, 1127 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1063 |
| AspS9I | GGNCC | 8 cut(s) | 46, 130, 142, 409, 598, 610, 898, 1209 |
| AsuC2I | CCSGG | 6 cut(s) | 279, 482, 768, 971, 1152, 1170 |
| AsuHPI | GGTGA | 7 cut(s) | 52, 253, 296, 520, 742, 785, 1208 |
| AvaI | CYCGRG | 1 cut(s) | 1018 |
| AvaII | GGWCC | 5 cut(s) | 130, 142, 598, 610, 1209 |
| BalI | TGGCCA | 2 cut(s) | 425, 914 |
| BamHI | GGATCC | 1 cut(s) | 1085 |
| BanI | GGYRCC | 4 cut(s) | 194, 662, 1164, 1172 |
| BanII | GRGCYC | 1 cut(s) | 43 |
| BbvI | GCAGC | 3 cut(s) | 315, 804, 1139 |
| BccI | CCATC | 2 cut(s) | 221, 710 |
| BceAI | ACGGC | 2 cut(s) | 650, 1221 |
| BciT130I | CCWGG | 8 cut(s) | 22, 44, 157, 315, 625, 804, 1202, 1207 |
| BcnI | CCSGG | 6 cut(s) | 279, 482, 768, 971, 1152, 1170 |
| BcoDI | GTCTC | 3 cut(s) | 281, 770, 1007 |
| BfaI | CTAG | 1 cut(s) | 36 |
| BfoI | RGCGCY | 2 cut(s) | 198, 666 |
| BfuAI | ACCTGC | 5 cut(s) | 14, 153, 409, 621, 898 |
| BglI | GCCNNNNNGGC | 5 cut(s) | 26, 315, 482, 804, 971 |
| Bme18I | GGWCC | 5 cut(s) | 130, 142, 598, 610, 1209 |
| BmeT110I | CYCGRG | 1 cut(s) | 1018 |
| BmgT120I | GGNCC | 8 cut(s) | 46, 130, 142, 409, 598, 610, 898, 1209 |
| BmiI | GGNNCC | 6 cut(s) | 196, 664, 677, 1087, 1166, 1174 |
| BmsI | GCATC | 5 cut(s) | 192, 252, 660, 741, 1114 |
| BpmI | CTGGAG | 2 cut(s) | 297, 786 |
| Bpu10I | CCTNAGC | 2 cut(s) | 179, 647 |
| BpuEI | CTTGAG | 1 cut(s) | 1031 |
| BpuMI | CCSGG | 6 cut(s) | 279, 482, 768, 971, 1152, 1170 |
| BsaHI | GRCGYC | 2 cut(s) | 195, 663 |
| BsaI | GGTCTC | 1 cut(s) | 1007 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 114, 144, 582, 612 |
| Bse118I | RCCGGY | 1 cut(s) | 1092 |
| Bse1I | ACTGG | 1 cut(s) | 1120 |
| BseBI | CCWGG | 8 cut(s) | 22, 44, 157, 315, 625, 804, 1202, 1207 |
| BseGI | GGATG | 3 cut(s) | 109, 577, 1129 |
| BseMII | CTCAG | 3 cut(s) | 170, 638, 1064 |
| BseNI | ACTGG | 1 cut(s) | 1120 |
| BsePI | GCGCGC | 2 cut(s) | 252, 741 |
| BseXI | GCAGC | 3 cut(s) | 315, 804, 1139 |
| BseYI | CCCAGC | 2 cut(s) | 232, 721 |
| BsgI | GTGCAG | 1 cut(s) | 981 |
| BshNI | GGYRCC | 4 cut(s) | 194, 662, 1164, 1172 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1018 |
| BsiSI | CCGG | 7 cut(s) | 279, 482, 768, 971, 1093, 1151, 1170 |
| BslFI | GGGAC | 3 cut(s) | 421, 481, 910 |
| BsmAI | GTCTC | 3 cut(s) | 281, 770, 1007 |
| BsmBI | CGTCTC | 2 cut(s) | 281, 770 |
| BsmFI | GGGAC | 3 cut(s) | 421, 481, 910 |
| Bso31I | GGTCTC | 1 cut(s) | 1007 |
| BsoBI | CYCGRG | 1 cut(s) | 1018 |
| Bsp1286I | GDGCHC | 1 cut(s) | 43 |
| BspCNI | CTCAG | 3 cut(s) | 171, 639, 1063 |
| BspLI | GGNNCC | 6 cut(s) | 196, 664, 677, 1087, 1166, 1174 |
| BspMI | ACCTGC | 5 cut(s) | 14, 153, 409, 621, 898 |
| BspPI | GGATC | 6 cut(s) | 168, 363, 636, 852, 1080, 1093 |
| BspT107I | GGYRCC | 4 cut(s) | 194, 662, 1164, 1172 |
| BspTNI | GGTCTC | 1 cut(s) | 1007 |
| BsrBI | CCGCTC | 2 cut(s) | 1047, 1107 |
| BsrFI | RCCGGY | 1 cut(s) | 1092 |
| BsrI | ACTGG | 1 cut(s) | 1120 |
| BssAI | RCCGGY | 1 cut(s) | 1092 |
| BssHII | GCGCGC | 2 cut(s) | 252, 741 |
| BssNI | GRCGYC | 2 cut(s) | 195, 663 |
| BssT1I | CCWWGG | 1 cut(s) | 511 |
| Bst2UI | CCWGG | 8 cut(s) | 22, 44, 157, 315, 625, 804, 1202, 1207 |
| BstACI | GRCGYC | 2 cut(s) | 195, 663 |
| BstC8I | GCNNGC | 6 cut(s) | 27, 254, 345, 743, 834, 1094 |
| BstDEI | CTNAG | 3 cut(s) | 179, 647, 1050 |
| BstF5I | GGATG | 3 cut(s) | 109, 577, 1129 |
| BstH2I | RGCGCY | 2 cut(s) | 198, 666 |
| BstMAI | GTCTC | 3 cut(s) | 281, 770, 1007 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 26, 249, 315, 482, 738, 804, 971 |
| BstNI | CCWGG | 8 cut(s) | 22, 44, 157, 315, 625, 804, 1202, 1207 |
| BstV1I | GCAGC | 3 cut(s) | 315, 804, 1139 |
| BstX2I | RGATCY | 3 cut(s) | 355, 844, 1085 |
| BstYI | RGATCY | 3 cut(s) | 355, 844, 1085 |
| BtgZI | GCGATG | 2 cut(s) | 199, 688 |
| BtsCI | GGATG | 3 cut(s) | 109, 577, 1129 |
| BtsI | GCAGTG | 1 cut(s) | 993 |
| BtsIMutI | CAGTG | 1 cut(s) | 993 |
| BveI | ACCTGC | 5 cut(s) | 14, 153, 409, 621, 898 |
| Cac8I | GCNNGC | 6 cut(s) | 27, 254, 345, 743, 834, 1094 |
| Cfr10I | RCCGGY | 1 cut(s) | 1092 |
| Cfr13I | GGNCC | 8 cut(s) | 46, 130, 142, 409, 598, 610, 898, 1209 |
| CpoI | CGGWCCG | 2 cut(s) | 130, 598 |
| CseI | GACGC | 3 cut(s) | 75, 543, 1186 |
| Csp6I | GTAC | 1 cut(s) | 504 |
| CspI | CGGWCCG | 2 cut(s) | 130, 598 |
| CviQI | GTAC | 1 cut(s) | 504 |
| DdeI | CTNAG | 3 cut(s) | 179, 647, 1050 |
| DinI | GGCGCC | 2 cut(s) | 196, 664 |
| EaeI | YGGCCR | 6 cut(s) | 18, 164, 423, 632, 912, 1234 |
| EciI | GGCGGA | 6 cut(s) | 122, 199, 590, 688, 999, 1170 |
| Eco130I | CCWWGG | 1 cut(s) | 511 |
| Eco24I | GRGCYC | 1 cut(s) | 43 |
| Eco31I | GGTCTC | 1 cut(s) | 1007 |
| Eco47I | GGWCC | 5 cut(s) | 130, 142, 598, 610, 1209 |
| Eco88I | CYCGRG | 1 cut(s) | 1018 |
| EcoRII | CCWGG | 8 cut(s) | 20, 42, 155, 313, 623, 802, 1200, 1205 |
| EcoT14I | CCWWGG | 1 cut(s) | 511 |
| EcoT38I | GRGCYC | 1 cut(s) | 43 |
| EgeI | GGCGCC | 2 cut(s) | 196, 664 |
| EheI | GGCGCC | 2 cut(s) | 196, 664 |
| ErhI | CCWWGG | 1 cut(s) | 511 |
| Esp3I | CGTCTC | 2 cut(s) | 281, 770 |
| FaiI | YATR | 4 cut(s) | 337, 339, 826, 828 |
| FaqI | GGGAC | 3 cut(s) | 421, 481, 910 |
| FauI | CCCGC | 2 cut(s) | 506, 1052 |
| FokI | GGATG | 3 cut(s) | 96, 564, 1136 |
| FriOI | GRGCYC | 1 cut(s) | 43 |
| FspBI | CTAG | 1 cut(s) | 36 |
| GsaI | CCCAGC | 2 cut(s) | 236, 725 |
| GsuI | CTGGAG | 2 cut(s) | 297, 786 |
| HaeII | RGCGCY | 2 cut(s) | 198, 666 |
| HapII | CCGG | 7 cut(s) | 279, 482, 768, 971, 1093, 1151, 1170 |
| HgaI | GACGC | 3 cut(s) | 75, 543, 1186 |
| Hin1I | GRCGYC | 2 cut(s) | 195, 663 |
| HinfI | GANTC | 6 cut(s) | 106, 494, 574, 987, 1059, 1158 |
| HpaII | CCGG | 7 cut(s) | 279, 482, 768, 971, 1093, 1151, 1170 |
| HphI | GGTGA | 7 cut(s) | 52, 253, 296, 520, 742, 785, 1208 |
| Hpy166II | GTNNAC | 2 cut(s) | 383, 872 |
| Hpy188I | TCNGA | 1 cut(s) | 142 |
| Hpy188III | TCNNGA | 2 cut(s) | 90, 558 |
| Hpy8I | GTNNAC | 2 cut(s) | 383, 872 |
| HpyAV | CCTTC | 3 cut(s) | 5, 147, 1026 |
| HpyCH4V | TGCA | 2 cut(s) | 998, 1127 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 26, 249, 315, 482, 738, 804, 971 |
| HpyF3I | CTNAG | 3 cut(s) | 179, 647, 1050 |
| Hsp92I | GRCGYC | 2 cut(s) | 195, 663 |
| KasI | GGCGCC | 2 cut(s) | 194, 662 |
| KroI | GCCGGC | 1 cut(s) | 1092 |
| KroNI | GCCGGC | 1 cut(s) | 1094 |
| Lsp1109I | GCAGC | 3 cut(s) | 315, 804, 1139 |
| LweI | GCATC | 5 cut(s) | 192, 252, 660, 741, 1114 |
| MaeI | CTAG | 1 cut(s) | 36 |
| MbiI | CCGCTC | 2 cut(s) | 1047, 1107 |
| MboII | GAAGA | 2 cut(s) | 350, 839 |
| MflI | RGATCY | 3 cut(s) | 355, 844, 1085 |
| MhlI | GDGCHC | 1 cut(s) | 43 |
| MlsI | TGGCCA | 2 cut(s) | 425, 914 |
| MluCI | AATT | 1 cut(s) | 1225 |
| MluNI | TGGCCA | 2 cut(s) | 425, 914 |
| Mly113I | GGCGCC | 2 cut(s) | 195, 663 |
| MlyI | GAGTC | 2 cut(s) | 503, 996 |
| MmeI | TCCRAC | 2 cut(s) | 137, 1114 |
| Mox20I | TGGCCA | 2 cut(s) | 425, 914 |
| MroNI | GCCGGC | 1 cut(s) | 1092 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1063 |
| MscI | TGGCCA | 2 cut(s) | 425, 914 |
| MseI | TTAA | 3 cut(s) | 452, 941, 1146 |
| MslI | CAYNNNNRTG | 2 cut(s) | 431, 920 |
| Msp20I | TGGCCA | 2 cut(s) | 425, 914 |
| MspA1I | CMGCKG | 5 cut(s) | 120, 236, 501, 725, 1070 |
| MspI | CCGG | 7 cut(s) | 279, 482, 768, 971, 1093, 1151, 1170 |
| MvaI | CCWGG | 8 cut(s) | 22, 44, 157, 315, 625, 804, 1202, 1207 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 26, 249, 315, 482, 738, 804, 971 |
| NaeI | GCCGGC | 1 cut(s) | 1094 |
| NarI | GGCGCC | 2 cut(s) | 195, 663 |
| NciI | CCSGG | 6 cut(s) | 279, 482, 768, 971, 1152, 1170 |
| NgoMIV | GCCGGC | 1 cut(s) | 1092 |
| NlaIV | GGNNCC | 6 cut(s) | 196, 664, 677, 1087, 1166, 1174 |
| NmeAIII | GCCGAG | 2 cut(s) | 430, 919 |
| OliI | CACNNNNGTG | 2 cut(s) | 431, 920 |
| PaqCI | CACCTGC | 2 cut(s) | 409, 898 |
| PasI | CCCWGGG | 1 cut(s) | 43 |
| PauI | GCGCGC | 2 cut(s) | 252, 741 |
| PdiI | GCCGGC | 1 cut(s) | 1094 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1063 |
| PfeI | GAWTC | 4 cut(s) | 106, 574, 1059, 1158 |
| PleI | GAGTC | 2 cut(s) | 502, 995 |
| PluTI | GGCGCC | 2 cut(s) | 198, 666 |
| PpsI | GAGTC | 2 cut(s) | 502, 995 |
| Psp6I | CCWGG | 8 cut(s) | 20, 42, 155, 313, 623, 802, 1200, 1205 |
| PspFI | CCCAGC | 2 cut(s) | 232, 721 |
| PspGI | CCWGG | 8 cut(s) | 20, 42, 155, 313, 623, 802, 1200, 1205 |
| PspN4I | GGNNCC | 6 cut(s) | 196, 664, 677, 1087, 1166, 1174 |
| PspPI | GGNCC | 8 cut(s) | 46, 130, 142, 409, 598, 610, 898, 1209 |
| PsuI | RGATCY | 3 cut(s) | 355, 844, 1085 |
| PteI | GCGCGC | 2 cut(s) | 252, 741 |
| RsaI | GTAC | 1 cut(s) | 505 |
| RsaNI | GTAC | 1 cut(s) | 504 |
| RseI | CAYNNNNRTG | 2 cut(s) | 431, 920 |
| Rsr2I | CGGWCCG | 2 cut(s) | 130, 598 |
| RsrII | CGGWCCG | 2 cut(s) | 130, 598 |
| SaqAI | TTAA | 3 cut(s) | 452, 941, 1146 |
| Sau96I | GGNCC | 8 cut(s) | 46, 130, 142, 409, 598, 610, 898, 1209 |
| SchI | GAGTC | 2 cut(s) | 503, 996 |
| SduI | GDGCHC | 1 cut(s) | 43 |
| SfaNI | GCATC | 5 cut(s) | 192, 252, 660, 741, 1114 |
| SfoI | GGCGCC | 2 cut(s) | 196, 664 |
| SinI | GGWCC | 5 cut(s) | 130, 142, 598, 610, 1209 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 431, 920 |
| SmlI | CTYRAG | 1 cut(s) | 1010 |
| SmoI | CTYRAG | 1 cut(s) | 1010 |
| Sse9I | AATT | 1 cut(s) | 1225 |
| SspDI | GGCGCC | 2 cut(s) | 194, 662 |
| SspMI | CTAG | 1 cut(s) | 36 |
| StyI | CCWWGG | 1 cut(s) | 511 |
| TaqI | TCGA | 1 cut(s) | 682 |
| TasI | AATT | 1 cut(s) | 1225 |
| TfiI | GAWTC | 4 cut(s) | 106, 574, 1059, 1158 |
| Tru1I | TTAA | 3 cut(s) | 452, 941, 1146 |
| Tru9I | TTAA | 3 cut(s) | 452, 941, 1146 |
| TscAI | CASTG | 1 cut(s) | 1000 |
| TseI | GCWGC | 3 cut(s) | 303, 792, 1127 |
| TspRI | CASTG | 1 cut(s) | 1000 |
| VpaK11BI | GGWCC | 5 cut(s) | 130, 142, 598, 610, 1209 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1063 |
| XspI | CTAG | 1 cut(s) | 36 |