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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 514 |
| Acc65I | GGTACC | 3 cut(s) | 4, 499, 526 |
| AccB1I | GGYRCC | 4 cut(s) | 4, 499, 526, 779 |
| AccBSI | CCGCTC | 2 cut(s) | 290, 613 |
| AccII | CGCG | 1 cut(s) | 460 |
| AclWI | GGATC | 2 cut(s) | 695, 832 |
| AcuI | CTGAAG | 1 cut(s) | 360 |
| AfaI | GTAC | 4 cut(s) | 6, 501, 516, 528 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 151, 322, 488 |
| AjnI | CCWGG | 4 cut(s) | 56, 467, 693, 820 |
| AluBI | AGCT | 5 cut(s) | 65, 142, 301, 409, 632 |
| AluI | AGCT | 5 cut(s) | 65, 142, 301, 409, 632 |
| Alw26I | GTCTC | 1 cut(s) | 596 |
| AlwI | GGATC | 2 cut(s) | 695, 832 |
| Ama87I | CYCGRG | 1 cut(s) | 72 |
| AoxI | GGCC | 4 cut(s) | 274, 360, 366, 479 |
| Asp718I | GGTACC | 3 cut(s) | 4, 499, 526 |
| AspLEI | GCGC | 4 cut(s) | 109, 351, 445, 672 |
| AspS9I | GGNCC | 6 cut(s) | 274, 367, 480, 506, 560, 679 |
| AsuC2I | CCSGG | 3 cut(s) | 237, 273, 504 |
| AsuHPI | GGTGA | 5 cut(s) | 254, 257, 277, 607, 823 |
| AvaI | CYCGRG | 1 cut(s) | 72 |
| AvaII | GGWCC | 3 cut(s) | 506, 560, 679 |
| BaeGI | GKGCMC | 1 cut(s) | 782 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 483, 516 |
| BanI | GGYRCC | 4 cut(s) | 4, 499, 526, 779 |
| BccI | CCATC | 3 cut(s) | 123, 550, 578 |
| BciT130I | CCWGG | 4 cut(s) | 58, 469, 695, 822 |
| BciVI | GTATCC | 1 cut(s) | 41 |
| BcnI | CCSGG | 3 cut(s) | 237, 273, 504 |
| BcoDI | GTCTC | 1 cut(s) | 596 |
| BfaI | CTAG | 3 cut(s) | 440, 633, 816 |
| BfmI | CTRYAG | 1 cut(s) | 66 |
| BfuAI | ACCTGC | 1 cut(s) | 514 |
| BfuI | GTATCC | 1 cut(s) | 41 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 130 |
| BglII | AGATCT | 1 cut(s) | 280 |
| BisI | GCNGC | 2 cut(s) | 110, 611 |
| BlsI | GCNGC | 2 cut(s) | 111, 612 |
| Bme1390I | CCNGG | 7 cut(s) | 58, 237, 273, 469, 504, 695, 822 |
| Bme18I | GGWCC | 3 cut(s) | 506, 560, 679 |
| BmeT110I | CYCGRG | 1 cut(s) | 72 |
| BmgT120I | GGNCC | 6 cut(s) | 274, 367, 480, 506, 560, 679 |
| BmiI | GGNNCC | 6 cut(s) | 6, 501, 507, 528, 561, 781 |
| BmrFI | CCNGG | 7 cut(s) | 58, 237, 273, 469, 504, 695, 822 |
| BmsI | GCATC | 5 cut(s) | 94, 142, 415, 510, 729 |
| BpmI | CTGGAG | 1 cut(s) | 770 |
| BpuMI | CCSGG | 3 cut(s) | 237, 273, 504 |
| BsaJI | CCNNGG | 4 cut(s) | 235, 363, 624, 665 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 151, 322, 488 |
| Bse118I | RCCGGY | 2 cut(s) | 477, 707 |
| Bse1I | ACTGG | 5 cut(s) | 312, 426, 493, 735, 753 |
| BseBI | CCWGG | 4 cut(s) | 58, 469, 695, 822 |
| BseDI | CCNNGG | 4 cut(s) | 235, 363, 624, 665 |
| BseGI | GGATG | 4 cut(s) | 430, 561, 570, 744 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 151, 322, 488 |
| BseMII | CTCAG | 2 cut(s) | 591, 636 |
| BseNI | ACTGG | 5 cut(s) | 312, 426, 493, 735, 753 |
| BseRI | GAGGAG | 1 cut(s) | 359 |
| BseSI | GKGCMC | 1 cut(s) | 782 |
| BseYI | CCCAGC | 2 cut(s) | 151, 226 |
| Bsh1236I | CGCG | 1 cut(s) | 460 |
| BshFI | GGCC | 4 cut(s) | 276, 362, 368, 481 |
| BshNI | GGYRCC | 4 cut(s) | 4, 499, 526, 779 |
| BsiHKCI | CYCGRG | 1 cut(s) | 72 |
| BsiSI | CCGG | 5 cut(s) | 237, 272, 478, 503, 708 |
| BslFI | GGGAC | 3 cut(s) | 157, 519, 573 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 151, 322, 488 |
| BsmAI | GTCTC | 1 cut(s) | 596 |
| BsmFI | GGGAC | 3 cut(s) | 157, 519, 573 |
| BsnI | GGCC | 4 cut(s) | 276, 362, 368, 481 |
| BsoBI | CYCGRG | 1 cut(s) | 72 |
| Bsp1286I | GDGCHC | 1 cut(s) | 782 |
| Bsp143I | GATC | 5 cut(s) | 93, 195, 280, 700, 824 |
| Bsp68I | TCGCGA | 1 cut(s) | 460 |
| BspANI | GGCC | 4 cut(s) | 276, 362, 368, 481 |
| BspCNI | CTCAG | 2 cut(s) | 592, 637 |
| BspFNI | CGCG | 1 cut(s) | 460 |
| BspLI | GGNNCC | 6 cut(s) | 6, 501, 507, 528, 561, 781 |
| BspMI | ACCTGC | 1 cut(s) | 514 |
| BspPI | GGATC | 2 cut(s) | 695, 832 |
| BspT107I | GGYRCC | 4 cut(s) | 4, 499, 526, 779 |
| BsrBI | CCGCTC | 2 cut(s) | 290, 613 |
| BsrFI | RCCGGY | 2 cut(s) | 477, 707 |
| BsrI | ACTGG | 5 cut(s) | 312, 426, 493, 735, 753 |
| BssAI | RCCGGY | 2 cut(s) | 477, 707 |
| BssECI | CCNNGG | 4 cut(s) | 235, 363, 624, 665 |
| BssMI | GATC | 5 cut(s) | 93, 195, 280, 700, 824 |
| BssT1I | CCWWGG | 1 cut(s) | 665 |
| Bst2UI | CCWGG | 4 cut(s) | 58, 469, 695, 822 |
| Bst6I | CTCTTC | 1 cut(s) | 336 |
| BstC8I | GCNNGC | 6 cut(s) | 63, 303, 452, 479, 660, 849 |
| BstDEI | CTNAG | 3 cut(s) | 254, 600, 645 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 320 |
| BstF5I | GGATG | 4 cut(s) | 430, 561, 570, 744 |
| BstFNI | CGCG | 1 cut(s) | 460 |
| BstHHI | GCGC | 4 cut(s) | 109, 351, 445, 672 |
| BstKTI | GATC | 5 cut(s) | 96, 198, 283, 703, 827 |
| BstMAI | GTCTC | 1 cut(s) | 596 |
| BstMBI | GATC | 5 cut(s) | 93, 195, 280, 700, 824 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 62, 130, 139, 148, 440, 451, 529 |
| BstNI | CCWGG | 4 cut(s) | 58, 469, 695, 822 |
| BstSCI | CCNGG | 7 cut(s) | 56, 235, 271, 467, 502, 693, 820 |
| BstSFI | CTRYAG | 1 cut(s) | 66 |
| BstSLI | GKGCMC | 1 cut(s) | 782 |
| BstUI | CGCG | 1 cut(s) | 460 |
| BstX2I | RGATCY | 2 cut(s) | 280, 700 |
| BstYI | RGATCY | 2 cut(s) | 280, 700 |
| BsuI | GTATCC | 1 cut(s) | 41 |
| BsuRI | GGCC | 4 cut(s) | 276, 362, 368, 481 |
| BtsCI | GGATG | 4 cut(s) | 430, 561, 570, 744 |
| BtsI | GCAGTG | 2 cut(s) | 605, 718 |
| BtsIMutI | CAGTG | 5 cut(s) | 305, 419, 486, 605, 718 |
| BtuMI | TCGCGA | 1 cut(s) | 460 |
| BveI | ACCTGC | 1 cut(s) | 514 |
| Cac8I | GCNNGC | 6 cut(s) | 63, 303, 452, 479, 660, 849 |
| CfoI | GCGC | 4 cut(s) | 109, 351, 445, 672 |
| Cfr10I | RCCGGY | 2 cut(s) | 477, 707 |
| Cfr13I | GGNCC | 6 cut(s) | 274, 367, 480, 506, 560, 679 |
| Csp6I | GTAC | 4 cut(s) | 5, 500, 515, 527 |
| CviAII | CATG | 1 cut(s) | 121 |
| CviQI | GTAC | 4 cut(s) | 5, 500, 515, 527 |
| DdeI | CTNAG | 3 cut(s) | 254, 600, 645 |
| DpnI | GATC | 5 cut(s) | 95, 197, 282, 702, 826 |
| DpnII | GATC | 5 cut(s) | 93, 195, 280, 700, 824 |
| Eam1104I | CTCTTC | 1 cut(s) | 336 |
| EarI | CTCTTC | 1 cut(s) | 336 |
| EciI | GGCGGA | 1 cut(s) | 645 |
| Eco130I | CCWWGG | 1 cut(s) | 665 |
| Eco47I | GGWCC | 3 cut(s) | 506, 560, 679 |
| Eco57I | CTGAAG | 1 cut(s) | 360 |
| Eco88I | CYCGRG | 1 cut(s) | 72 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 320 |
| EcoRII | CCWGG | 4 cut(s) | 56, 467, 693, 820 |
| EcoT14I | CCWWGG | 1 cut(s) | 665 |
| ErhI | CCWWGG | 1 cut(s) | 665 |
| FaeI | CATG | 1 cut(s) | 124 |
| FaiI | YATR | 5 cut(s) | 122, 168, 216, 297, 845 |
| FaqI | GGGAC | 3 cut(s) | 157, 519, 573 |
| FatI | CATG | 1 cut(s) | 120 |
| Fnu4HI | GCNGC | 2 cut(s) | 110, 611 |
| FokI | GGATG | 4 cut(s) | 437, 557, 568, 751 |
| Fsp4HI | GCNGC | 2 cut(s) | 110, 611 |
| FspBI | CTAG | 3 cut(s) | 440, 633, 816 |
| GlaI | GCGC | 4 cut(s) | 108, 350, 444, 671 |
| GluI | GCNGC | 2 cut(s) | 110, 611 |
| GsaI | CCCAGC | 2 cut(s) | 155, 230 |
| GsuI | CTGGAG | 1 cut(s) | 770 |
| HaeIII | GGCC | 4 cut(s) | 276, 362, 368, 481 |
| HapII | CCGG | 5 cut(s) | 237, 272, 478, 503, 708 |
| HhaI | GCGC | 4 cut(s) | 109, 351, 445, 672 |
| Hin1II | CATG | 1 cut(s) | 124 |
| Hin6I | GCGC | 4 cut(s) | 107, 349, 443, 670 |
| HinP1I | GCGC | 4 cut(s) | 107, 349, 443, 670 |
| HinfI | GANTC | 2 cut(s) | 156, 773 |
| HpaII | CCGG | 5 cut(s) | 237, 272, 478, 503, 708 |
| HphI | GGTGA | 5 cut(s) | 254, 257, 277, 607, 823 |
| Hpy188I | TCNGA | 3 cut(s) | 138, 628, 857 |
| Hpy188III | TCNNGA | 2 cut(s) | 459, 811 |
| HpyAV | CCTTC | 3 cut(s) | 212, 326, 352 |
| HpyCH4IV | ACGT | 2 cut(s) | 397, 494 |
| HpyCH4V | TGCA | 6 cut(s) | 428, 523, 716, 724, 742, 847 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 62, 130, 139, 148, 440, 451, 529 |
| HpyF3I | CTNAG | 3 cut(s) | 254, 600, 645 |
| HpySE526I | ACGT | 2 cut(s) | 397, 494 |
| Hsp92II | CATG | 1 cut(s) | 124 |
| HspAI | GCGC | 4 cut(s) | 107, 349, 443, 670 |
| KpnI | GGTACC | 3 cut(s) | 8, 503, 530 |
| KroI | GCCGGC | 1 cut(s) | 477 |
| KroNI | GCCGGC | 1 cut(s) | 479 |
| Kzo9I | GATC | 5 cut(s) | 93, 195, 280, 700, 824 |
| LmnI | GCTCC | 4 cut(s) | 147, 346, 589, 751 |
| LweI | GCATC | 5 cut(s) | 94, 142, 415, 510, 729 |
| MaeI | CTAG | 3 cut(s) | 440, 633, 816 |
| MaeII | ACGT | 2 cut(s) | 397, 494 |
| MaeIII | GTNAC | 4 cut(s) | 37, 263, 393, 811 |
| MalI | GATC | 5 cut(s) | 95, 197, 282, 702, 826 |
| MbiI | CCGCTC | 2 cut(s) | 290, 613 |
| MboI | GATC | 5 cut(s) | 93, 195, 280, 700, 824 |
| MboII | GAAGA | 2 cut(s) | 205, 353 |
| MflI | RGATCY | 2 cut(s) | 280, 700 |
| MhlI | GDGCHC | 1 cut(s) | 782 |
| MluCI | AATT | 1 cut(s) | 82 |
| MlyI | GAGTC | 1 cut(s) | 782 |
| MmeI | TCCRAC | 1 cut(s) | 567 |
| MroNI | GCCGGC | 1 cut(s) | 477 |
| MspA1I | CMGCKG | 1 cut(s) | 532 |
| MspI | CCGG | 5 cut(s) | 237, 272, 478, 503, 708 |
| MspR9I | CCNGG | 7 cut(s) | 58, 237, 273, 469, 504, 695, 822 |
| MvaI | CCWGG | 4 cut(s) | 58, 469, 695, 822 |
| MvnI | CGCG | 1 cut(s) | 460 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 62, 130, 139, 148, 440, 451, 529 |
| NaeI | GCCGGC | 1 cut(s) | 479 |
| NciI | CCSGG | 3 cut(s) | 237, 273, 504 |
| NdeII | GATC | 5 cut(s) | 93, 195, 280, 700, 824 |
| NgoMIV | GCCGGC | 1 cut(s) | 477 |
| NlaIII | CATG | 1 cut(s) | 124 |
| NlaIV | GGNNCC | 6 cut(s) | 6, 501, 507, 528, 561, 781 |
| NmeAIII | GCCGAG | 1 cut(s) | 388 |
| NmuCI | GTSAC | 4 cut(s) | 37, 263, 393, 811 |
| NruI | TCGCGA | 1 cut(s) | 460 |
| PdiI | GCCGGC | 1 cut(s) | 479 |
| PfeI | GAWTC | 1 cut(s) | 156 |
| PkrI | GCNGC | 2 cut(s) | 111, 612 |
| PleI | GAGTC | 1 cut(s) | 781 |
| PpsI | GAGTC | 1 cut(s) | 781 |
| Psp6I | CCWGG | 4 cut(s) | 56, 467, 693, 820 |
| PspFI | CCCAGC | 2 cut(s) | 151, 226 |
| PspGI | CCWGG | 4 cut(s) | 56, 467, 693, 820 |
| PspN4I | GGNNCC | 6 cut(s) | 6, 501, 507, 528, 561, 781 |
| PspPI | GGNCC | 6 cut(s) | 274, 367, 480, 506, 560, 679 |
| PsuI | RGATCY | 2 cut(s) | 280, 700 |
| RruI | TCGCGA | 1 cut(s) | 460 |
| RsaI | GTAC | 4 cut(s) | 6, 501, 516, 528 |
| RsaNI | GTAC | 4 cut(s) | 5, 500, 515, 527 |
| SatI | GCNGC | 2 cut(s) | 110, 611 |
| Sau3AI | GATC | 5 cut(s) | 93, 195, 280, 700, 824 |
| Sau96I | GGNCC | 6 cut(s) | 274, 367, 480, 506, 560, 679 |
| SchI | GAGTC | 1 cut(s) | 782 |
| ScrFI | CCNGG | 7 cut(s) | 58, 237, 273, 469, 504, 695, 822 |
| SduI | GDGCHC | 1 cut(s) | 782 |
| SfaNI | GCATC | 5 cut(s) | 94, 142, 415, 510, 729 |
| SfcI | CTRYAG | 1 cut(s) | 66 |
| SinI | GGWCC | 3 cut(s) | 506, 560, 679 |
| Sse9I | AATT | 1 cut(s) | 82 |
| SspMI | CTAG | 3 cut(s) | 440, 633, 816 |
| StyD4I | CCNGG | 7 cut(s) | 56, 235, 271, 467, 502, 693, 820 |
| StyI | CCWWGG | 1 cut(s) | 665 |
| TaiI | ACGT | 2 cut(s) | 400, 497 |
| TaqI | TCGA | 3 cut(s) | 159, 180, 551 |
| TasI | AATT | 1 cut(s) | 82 |
| TauI | GCSGC | 2 cut(s) | 112, 613 |
| TfiI | GAWTC | 1 cut(s) | 156 |
| TscAI | CASTG | 5 cut(s) | 312, 426, 493, 612, 718 |
| TseFI | GTSAC | 4 cut(s) | 37, 263, 393, 811 |
| Tsp45I | GTSAC | 4 cut(s) | 37, 263, 393, 811 |
| TspGWI | ACGGA | 2 cut(s) | 60, 755 |
| TspRI | CASTG | 5 cut(s) | 312, 426, 493, 612, 718 |
| VpaK11BI | GGWCC | 3 cut(s) | 506, 560, 679 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 320 |
| XspI | CTAG | 3 cut(s) | 440, 633, 816 |