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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 218 |
| Acc65I | GGTACC | 2 cut(s) | 203, 230 |
| AccB1I | GGYRCC | 2 cut(s) | 203, 230 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 434 |
| AccBSI | CCGCTC | 1 cut(s) | 328 |
| AccI | GTMKAC | 1 cut(s) | 182 |
| AciI | CCGC | 7 cut(s) | 15, 136, 270, 315, 326, 360, 389 |
| AclWI | GGATC | 1 cut(s) | 25 |
| AcuI | CTGAAG | 2 cut(s) | 88, 370 |
| AfaI | GTAC | 2 cut(s) | 205, 232 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 192, 332, 434 |
| AflIII | ACRYGT | 1 cut(s) | 99 |
| AjnI | CCWGG | 2 cut(s) | 171, 206 |
| AluBI | AGCT | 1 cut(s) | 5 |
| AluI | AGCT | 1 cut(s) | 5 |
| AlwI | GGATC | 1 cut(s) | 25 |
| AoxI | GGCC | 3 cut(s) | 130, 210, 378 |
| Asp718I | GGTACC | 2 cut(s) | 203, 230 |
| AspLEI | GCGC | 2 cut(s) | 55, 376 |
| AspS9I | GGNCC | 2 cut(s) | 210, 264 |
| AsuC2I | CCSGG | 1 cut(s) | 386 |
| AsuNHI | GCTAGC | 1 cut(s) | 5 |
| AvaII | GGWCC | 1 cut(s) | 264 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 187, 220 |
| BanI | GGYRCC | 2 cut(s) | 203, 230 |
| BbsI | GAAGAC | 1 cut(s) | 126 |
| BceAI | ACGGC | 1 cut(s) | 365 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 405, 439 |
| BciT130I | CCWGG | 2 cut(s) | 173, 208 |
| BcnI | CCSGG | 1 cut(s) | 386 |
| BfaI | CTAG | 2 cut(s) | 6, 337 |
| BfuAI | ACCTGC | 1 cut(s) | 218 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 386 |
| Bme1390I | CCNGG | 3 cut(s) | 173, 208, 386 |
| Bme18I | GGWCC | 1 cut(s) | 264 |
| BmgT120I | GGNCC | 2 cut(s) | 210, 264 |
| BmiI | GGNNCC | 3 cut(s) | 205, 232, 265 |
| BmrFI | CCNGG | 3 cut(s) | 173, 208, 386 |
| BmsI | GCATC | 3 cut(s) | 214, 407, 433 |
| BmtI | GCTAGC | 1 cut(s) | 9 |
| BpiI | GAAGAC | 1 cut(s) | 126 |
| BpmI | CTGGAG | 1 cut(s) | 147 |
| BpuMI | CCSGG | 1 cut(s) | 386 |
| BsaAI | YACGTR | 1 cut(s) | 165 |
| BsaJI | CCNNGG | 2 cut(s) | 207, 369 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 192, 332, 434 |
| Bse118I | RCCGGY | 2 cut(s) | 192, 212 |
| Bse1I | ACTGG | 2 cut(s) | 16, 457 |
| BseBI | CCWGG | 2 cut(s) | 173, 208 |
| BseDI | CCNNGG | 2 cut(s) | 207, 369 |
| BseGI | GGATG | 1 cut(s) | 448 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 192, 332, 434 |
| BseMII | CTCAG | 1 cut(s) | 238 |
| BseNI | ACTGG | 2 cut(s) | 16, 457 |
| BseRI | GAGGAG | 2 cut(s) | 60, 63 |
| BseYI | CCCAGC | 2 cut(s) | 143, 186 |
| BshFI | GGCC | 3 cut(s) | 132, 212, 380 |
| BshNI | GGYRCC | 2 cut(s) | 203, 230 |
| BsiSI | CCGG | 3 cut(s) | 193, 213, 386 |
| BslFI | GGGAC | 2 cut(s) | 277, 368 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 192, 332, 434 |
| BsmFI | GGGAC | 2 cut(s) | 277, 368 |
| BsnI | GGCC | 3 cut(s) | 132, 212, 380 |
| Bsp143I | GATC | 1 cut(s) | 17 |
| Bsp19I | CCATGG | 1 cut(s) | 369 |
| BspACI | CCGC | 7 cut(s) | 15, 136, 270, 315, 326, 360, 389 |
| BspANI | GGCC | 3 cut(s) | 132, 212, 380 |
| BspCNI | CTCAG | 1 cut(s) | 239 |
| BspHI | TCATGA | 1 cut(s) | 94 |
| BspLI | GGNNCC | 3 cut(s) | 205, 232, 265 |
| BspMI | ACCTGC | 1 cut(s) | 218 |
| BspOI | GCTAGC | 1 cut(s) | 9 |
| BspPI | GGATC | 1 cut(s) | 25 |
| BspT107I | GGYRCC | 2 cut(s) | 203, 230 |
| BsrBI | CCGCTC | 1 cut(s) | 328 |
| BsrFI | RCCGGY | 2 cut(s) | 192, 212 |
| BsrI | ACTGG | 2 cut(s) | 16, 457 |
| BssAI | RCCGGY | 2 cut(s) | 192, 212 |
| BssECI | CCNNGG | 2 cut(s) | 207, 369 |
| BssMI | GATC | 1 cut(s) | 17 |
| BssT1I | CCWWGG | 1 cut(s) | 369 |
| Bst2UI | CCWGG | 2 cut(s) | 173, 208 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 417 |
| BstBAI | YACGTR | 1 cut(s) | 165 |
| BstC8I | GCNNGC | 3 cut(s) | 7, 156, 378 |
| BstDEI | CTNAG | 1 cut(s) | 247 |
| BstDSI | CCRYGG | 1 cut(s) | 369 |
| BstF5I | GGATG | 1 cut(s) | 448 |
| BstHHI | GCGC | 2 cut(s) | 55, 376 |
| BstKTI | GATC | 1 cut(s) | 20 |
| BstMBI | GATC | 1 cut(s) | 17 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 144, 386, 408, 417 |
| BstNI | CCWGG | 2 cut(s) | 173, 208 |
| BstSCI | CCNGG | 3 cut(s) | 171, 206, 384 |
| BstV2I | GAAGAC | 1 cut(s) | 126 |
| BstX2I | RGATCY | 1 cut(s) | 17 |
| BstYI | RGATCY | 1 cut(s) | 17 |
| BsuRI | GGCC | 3 cut(s) | 132, 212, 380 |
| BtgI | CCRYGG | 1 cut(s) | 369 |
| BtsCI | GGATG | 1 cut(s) | 448 |
| BtsIMutI | CAGTG | 2 cut(s) | 9, 234 |
| BveI | ACCTGC | 1 cut(s) | 218 |
| Cac8I | GCNNGC | 3 cut(s) | 7, 156, 378 |
| CciI | TCATGA | 1 cut(s) | 94 |
| CfoI | GCGC | 2 cut(s) | 55, 376 |
| Cfr10I | RCCGGY | 2 cut(s) | 192, 212 |
| Cfr13I | GGNCC | 2 cut(s) | 210, 264 |
| CseI | GACGC | 1 cut(s) | 131 |
| Csp6I | GTAC | 2 cut(s) | 204, 231 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 232, 267 |
| CviAII | CATG | 2 cut(s) | 95, 370 |
| CviJI | RGCY | 9 cut(s) | 5, 66, 72, 87, 132, 212, 336, 380, 402 |
| CviKI_1 | RGCY | 9 cut(s) | 5, 66, 72, 87, 132, 212, 336, 380, 402 |
| CviQI | GTAC | 2 cut(s) | 204, 231 |
| DdeI | CTNAG | 1 cut(s) | 247 |
| DpnI | GATC | 1 cut(s) | 19 |
| DpnII | GATC | 1 cut(s) | 17 |
| EciI | GGCGGA | 2 cut(s) | 30, 349 |
| Eco130I | CCWWGG | 1 cut(s) | 369 |
| Eco47I | GGWCC | 1 cut(s) | 264 |
| Eco57I | CTGAAG | 2 cut(s) | 88, 370 |
| EcoRII | CCWGG | 2 cut(s) | 171, 206 |
| EcoT14I | CCWWGG | 1 cut(s) | 369 |
| ErhI | CCWWGG | 1 cut(s) | 369 |
| FaeI | CATG | 2 cut(s) | 98, 373 |
| FaiI | YATR | 5 cut(s) | 25, 96, 219, 276, 371 |
| FaqI | GGGAC | 2 cut(s) | 277, 368 |
| FatI | CATG | 2 cut(s) | 94, 369 |
| FauI | CCCGC | 2 cut(s) | 322, 382 |
| FblI | GTMKAC | 1 cut(s) | 182 |
| FokI | GGATG | 1 cut(s) | 455 |
| FspBI | CTAG | 2 cut(s) | 6, 337 |
| GlaI | GCGC | 2 cut(s) | 54, 375 |
| GsaI | CCCAGC | 2 cut(s) | 147, 190 |
| GsuI | CTGGAG | 1 cut(s) | 147 |
| HaeIII | GGCC | 3 cut(s) | 132, 212, 380 |
| HapII | CCGG | 3 cut(s) | 193, 213, 386 |
| HgaI | GACGC | 1 cut(s) | 131 |
| HhaI | GCGC | 2 cut(s) | 55, 376 |
| Hin1II | CATG | 2 cut(s) | 98, 373 |
| Hin6I | GCGC | 2 cut(s) | 53, 374 |
| HinP1I | GCGC | 2 cut(s) | 53, 374 |
| HincII | GTYRAC | 1 cut(s) | 183 |
| HindII | GTYRAC | 1 cut(s) | 183 |
| HinfI | GANTC | 1 cut(s) | 306 |
| HpaII | CCGG | 3 cut(s) | 193, 213, 386 |
| Hpy166II | GTNNAC | 1 cut(s) | 183 |
| Hpy188I | TCNGA | 1 cut(s) | 332 |
| Hpy188III | TCNNGA | 1 cut(s) | 95 |
| Hpy8I | GTNNAC | 1 cut(s) | 183 |
| HpyAV | CCTTC | 1 cut(s) | 345 |
| HpyCH4IV | ACGT | 3 cut(s) | 41, 101, 164 |
| HpyCH4V | TGCA | 3 cut(s) | 227, 420, 446 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 144, 386, 408, 417 |
| HpyF3I | CTNAG | 1 cut(s) | 247 |
| HpySE526I | ACGT | 3 cut(s) | 41, 101, 164 |
| Hsp92II | CATG | 2 cut(s) | 98, 373 |
| HspAI | GCGC | 2 cut(s) | 53, 374 |
| KpnI | GGTACC | 2 cut(s) | 207, 234 |
| Kzo9I | GATC | 1 cut(s) | 17 |
| LmnI | GCTCC | 1 cut(s) | 50 |
| LweI | GCATC | 3 cut(s) | 214, 407, 433 |
| MaeI | CTAG | 2 cut(s) | 6, 337 |
| MaeII | ACGT | 3 cut(s) | 41, 101, 164 |
| MaeIII | GTNAC | 1 cut(s) | 160 |
| MalI | GATC | 1 cut(s) | 19 |
| MbiI | CCGCTC | 1 cut(s) | 328 |
| MboI | GATC | 1 cut(s) | 17 |
| MboII | GAAGA | 2 cut(s) | 12, 131 |
| MflI | RGATCY | 1 cut(s) | 17 |
| MmeI | TCCRAC | 1 cut(s) | 415 |
| MnlI | CCTC | 9 cut(s) | 38, 41, 77, 101, 122, 150, 293, 326, 337 |
| MslI | CAYNNNNRTG | 1 cut(s) | 222 |
| MspI | CCGG | 3 cut(s) | 193, 213, 386 |
| MspR9I | CCNGG | 3 cut(s) | 173, 208, 386 |
| MvaI | CCWGG | 2 cut(s) | 173, 208 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 144, 386, 408, 417 |
| NciI | CCSGG | 1 cut(s) | 386 |
| NcoI | CCATGG | 1 cut(s) | 369 |
| NdeII | GATC | 1 cut(s) | 17 |
| NheI | GCTAGC | 1 cut(s) | 5 |
| NlaIII | CATG | 2 cut(s) | 98, 373 |
| NlaIV | GGNNCC | 3 cut(s) | 205, 232, 265 |
| NmuCI | GTSAC | 1 cut(s) | 160 |
| PagI | TCATGA | 1 cut(s) | 94 |
| PfeI | GAWTC | 1 cut(s) | 306 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 434 |
| Ppu21I | YACGTR | 1 cut(s) | 165 |
| Psp6I | CCWGG | 2 cut(s) | 171, 206 |
| PspFI | CCCAGC | 2 cut(s) | 143, 186 |
| PspGI | CCWGG | 2 cut(s) | 171, 206 |
| PspN4I | GGNNCC | 3 cut(s) | 205, 232, 265 |
| PspPI | GGNCC | 2 cut(s) | 210, 264 |
| PsuI | RGATCY | 1 cut(s) | 17 |
| RsaI | GTAC | 2 cut(s) | 205, 232 |
| RsaNI | GTAC | 2 cut(s) | 204, 231 |
| RseI | CAYNNNNRTG | 1 cut(s) | 222 |
| SalI | GTCGAC | 1 cut(s) | 181 |
| Sau3AI | GATC | 1 cut(s) | 17 |
| Sau96I | GGNCC | 2 cut(s) | 210, 264 |
| ScrFI | CCNGG | 3 cut(s) | 173, 208, 386 |
| SetI | ASST | 6 cut(s) | 7, 44, 104, 167, 232, 248 |
| SfaNI | GCATC | 3 cut(s) | 214, 407, 433 |
| SinI | GGWCC | 1 cut(s) | 264 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 222 |
| SsiI | CCGC | 7 cut(s) | 15, 136, 270, 315, 326, 360, 389 |
| SspMI | CTAG | 2 cut(s) | 6, 337 |
| StyD4I | CCNGG | 3 cut(s) | 171, 206, 384 |
| StyI | CCWWGG | 1 cut(s) | 369 |
| TaiI | ACGT | 3 cut(s) | 44, 104, 167 |
| TaqI | TCGA | 1 cut(s) | 182 |
| TfiI | GAWTC | 1 cut(s) | 306 |
| TscAI | CASTG | 2 cut(s) | 16, 241 |
| TseFI | GTSAC | 1 cut(s) | 160 |
| Tsp45I | GTSAC | 1 cut(s) | 160 |
| TspDTI | ATGAA | 1 cut(s) | 12 |
| TspRI | CASTG | 2 cut(s) | 16, 241 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 434 |
| VpaK11BI | GGWCC | 1 cut(s) | 264 |
| XmiI | GTMKAC | 1 cut(s) | 182 |
| XspI | CTAG | 2 cut(s) | 6, 337 |