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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 259 |
| Acc36I | ACCTGC | 1 cut(s) | 259 |
| AccB1I | GGYRCC | 1 cut(s) | 501 |
| AccBSI | CCGCTC | 1 cut(s) | 527 |
| AccII | CGCG | 3 cut(s) | 74, 231, 352 |
| AclI | AACGTT | 1 cut(s) | 99 |
| AclWI | GGATC | 2 cut(s) | 48, 180 |
| AcoI | YGGCCR | 1 cut(s) | 273 |
| AcsI | RAATTY | 1 cut(s) | 290 |
| AcyI | GRCGYC | 1 cut(s) | 502 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 268, 380, 494 |
| AgsI | TTSAA | 1 cut(s) | 295 |
| AjnI | CCWGG | 2 cut(s) | 168, 415 |
| AluBI | AGCT | 3 cut(s) | 193, 326, 398 |
| AluI | AGCT | 3 cut(s) | 193, 326, 398 |
| Alw21I | GWGCWC | 2 cut(s) | 243, 359 |
| Alw26I | GTCTC | 1 cut(s) | 427 |
| AlwI | GGATC | 2 cut(s) | 48, 180 |
| AoxI | GGCC | 4 cut(s) | 252, 258, 273, 423 |
| ApaI | GGGCCC | 1 cut(s) | 427 |
| ApeKI | GCWGC | 1 cut(s) | 131 |
| ApoI | RAATTY | 1 cut(s) | 290 |
| AspLEI | GCGC | 2 cut(s) | 337, 504 |
| AspS9I | GGNCC | 3 cut(s) | 259, 423, 424 |
| AsuHPI | GGTGA | 1 cut(s) | 545 |
| BaeGI | GKGCMC | 1 cut(s) | 427 |
| BalI | TGGCCA | 1 cut(s) | 275 |
| BanI | GGYRCC | 1 cut(s) | 501 |
| BanII | GRGCYC | 1 cut(s) | 427 |
| Bbv12I | GWGCWC | 2 cut(s) | 243, 359 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 1 cut(s) | 143 |
| BccI | CCATC | 1 cut(s) | 307 |
| BciT130I | CCWGG | 2 cut(s) | 170, 417 |
| BcoDI | GTCTC | 1 cut(s) | 427 |
| BfaI | CTAG | 2 cut(s) | 453, 559 |
| BfmI | CTRYAG | 1 cut(s) | 490 |
| BfoI | RGCGCY | 1 cut(s) | 505 |
| BfuAI | ACCTGC | 1 cut(s) | 259 |
| BisI | GCNGC | 2 cut(s) | 75, 132 |
| BlsI | GCNGC | 2 cut(s) | 76, 133 |
| Bme1390I | CCNGG | 2 cut(s) | 170, 417 |
| BmgT120I | GGNCC | 3 cut(s) | 259, 423, 424 |
| BmiI | GGNNCC | 2 cut(s) | 425, 503 |
| BmrFI | CCNGG | 2 cut(s) | 170, 417 |
| BmrI | ACTGGG | 1 cut(s) | 83 |
| BmuI | ACTGGG | 1 cut(s) | 83 |
| BpmI | CTGGAG | 2 cut(s) | 399, 451 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BsaHI | GRCGYC | 1 cut(s) | 502 |
| BsaJI | CCNNGG | 2 cut(s) | 72, 255 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 268, 380, 494 |
| Bse118I | RCCGGY | 1 cut(s) | 380 |
| Bse1I | ACTGG | 1 cut(s) | 89 |
| BseBI | CCWGG | 2 cut(s) | 170, 417 |
| BseDI | CCNNGG | 2 cut(s) | 72, 255 |
| BseGI | GGATG | 1 cut(s) | 466 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 268, 380, 494 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseNI | ACTGG | 1 cut(s) | 89 |
| BseRI | GAGGAG | 3 cut(s) | 44, 169, 263 |
| BseSI | GKGCMC | 1 cut(s) | 427 |
| BseXI | GCAGC | 1 cut(s) | 143 |
| BseYI | CCCAGC | 1 cut(s) | 331 |
| Bsh1236I | CGCG | 3 cut(s) | 74, 231, 352 |
| BshFI | GGCC | 4 cut(s) | 254, 260, 275, 425 |
| BshNI | GGYRCC | 1 cut(s) | 501 |
| BsiHKAI | GWGCWC | 2 cut(s) | 243, 359 |
| BsiSI | CCGG | 1 cut(s) | 381 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 268, 380, 494 |
| BsmAI | GTCTC | 1 cut(s) | 427 |
| BsnI | GGCC | 4 cut(s) | 254, 260, 275, 425 |
| Bsp120I | GGGCCC | 1 cut(s) | 423 |
| Bsp1286I | GDGCHC | 3 cut(s) | 243, 359, 427 |
| Bsp143I | GATC | 2 cut(s) | 40, 172 |
| BspANI | GGCC | 4 cut(s) | 254, 260, 275, 425 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspFNI | CGCG | 3 cut(s) | 74, 231, 352 |
| BspLI | GGNNCC | 2 cut(s) | 425, 503 |
| BspMAI | CTGCAG | 1 cut(s) | 494 |
| BspMI | ACCTGC | 1 cut(s) | 259 |
| BspPI | GGATC | 2 cut(s) | 48, 180 |
| BspT107I | GGYRCC | 1 cut(s) | 501 |
| BsrBI | CCGCTC | 1 cut(s) | 527 |
| BsrFI | RCCGGY | 1 cut(s) | 380 |
| BsrI | ACTGG | 1 cut(s) | 89 |
| BssAI | RCCGGY | 1 cut(s) | 380 |
| BssECI | CCNNGG | 2 cut(s) | 72, 255 |
| BssMI | GATC | 2 cut(s) | 40, 172 |
| BssNI | GRCGYC | 1 cut(s) | 502 |
| Bst2UI | CCWGG | 2 cut(s) | 170, 417 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 155 |
| BstACI | GRCGYC | 1 cut(s) | 502 |
| BstC8I | GCNNGC | 3 cut(s) | 195, 427, 500 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstDSI | CCRYGG | 1 cut(s) | 72 |
| BstF5I | GGATG | 1 cut(s) | 466 |
| BstFNI | CGCG | 3 cut(s) | 74, 231, 352 |
| BstH2I | RGCGCY | 1 cut(s) | 505 |
| BstHHI | GCGC | 2 cut(s) | 337, 504 |
| BstKTI | GATC | 2 cut(s) | 43, 175 |
| BstMAI | GTCTC | 1 cut(s) | 427 |
| BstMBI | GATC | 2 cut(s) | 40, 172 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 137, 156, 165, 317, 332, 343, 404, 426, 473 |
| BstNI | CCWGG | 2 cut(s) | 170, 417 |
| BstSCI | CCNGG | 2 cut(s) | 168, 415 |
| BstSFI | CTRYAG | 1 cut(s) | 490 |
| BstSLI | GKGCMC | 1 cut(s) | 427 |
| BstUI | CGCG | 3 cut(s) | 74, 231, 352 |
| BstV1I | GCAGC | 1 cut(s) | 143 |
| BstX2I | RGATCY | 1 cut(s) | 172 |
| BstYI | RGATCY | 1 cut(s) | 172 |
| BsuRI | GGCC | 4 cut(s) | 254, 260, 275, 425 |
| BtgI | CCRYGG | 1 cut(s) | 72 |
| BtgZI | GCGATG | 1 cut(s) | 326 |
| BtsCI | GGATG | 1 cut(s) | 466 |
| BtsIMutI | CAGTG | 1 cut(s) | 96 |
| BveI | ACCTGC | 1 cut(s) | 259 |
| Cac8I | GCNNGC | 3 cut(s) | 195, 427, 500 |
| CfoI | GCGC | 2 cut(s) | 337, 504 |
| Cfr10I | RCCGGY | 1 cut(s) | 380 |
| Cfr13I | GGNCC | 3 cut(s) | 259, 423, 424 |
| Cfr42I | CCGCGG | 1 cut(s) | 75 |
| CseI | GACGC | 1 cut(s) | 523 |
| CviJI | RGCY | 9 cut(s) | 77, 168, 193, 254, 260, 275, 326, 398, 425 |
| CviKI_1 | RGCY | 9 cut(s) | 77, 168, 193, 254, 260, 275, 326, 398, 425 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DinI | GGCGCC | 1 cut(s) | 503 |
| DpnI | GATC | 2 cut(s) | 42, 174 |
| DpnII | GATC | 2 cut(s) | 40, 172 |
| EaeI | YGGCCR | 1 cut(s) | 273 |
| EciI | GGCGGA | 1 cut(s) | 218 |
| Eco24I | GRGCYC | 1 cut(s) | 427 |
| EcoO109I | RGGNCCY | 1 cut(s) | 423 |
| EcoRII | CCWGG | 2 cut(s) | 168, 415 |
| EcoT38I | GRGCYC | 1 cut(s) | 427 |
| EgeI | GGCGCC | 1 cut(s) | 503 |
| EheI | GGCGCC | 1 cut(s) | 503 |
| FaiI | YATR | 4 cut(s) | 185, 189, 213, 245 |
| FauI | CCCGC | 3 cut(s) | 8, 79, 434 |
| Fnu4HI | GCNGC | 2 cut(s) | 75, 132 |
| FokI | GGATG | 1 cut(s) | 473 |
| FriOI | GRGCYC | 1 cut(s) | 427 |
| Fsp4HI | GCNGC | 2 cut(s) | 75, 132 |
| FspBI | CTAG | 2 cut(s) | 453, 559 |
| GlaI | GCGC | 2 cut(s) | 336, 503 |
| GluI | GCNGC | 2 cut(s) | 75, 132 |
| GsaI | CCCAGC | 1 cut(s) | 335 |
| GsuI | CTGGAG | 2 cut(s) | 399, 451 |
| HaeII | RGCGCY | 1 cut(s) | 505 |
| HaeIII | GGCC | 4 cut(s) | 254, 260, 275, 425 |
| HapII | CCGG | 1 cut(s) | 381 |
| HgaI | GACGC | 1 cut(s) | 523 |
| HhaI | GCGC | 2 cut(s) | 337, 504 |
| Hin1I | GRCGYC | 1 cut(s) | 502 |
| Hin6I | GCGC | 2 cut(s) | 335, 502 |
| HinP1I | GCGC | 2 cut(s) | 335, 502 |
| HincII | GTYRAC | 2 cut(s) | 97, 371 |
| HindII | GTYRAC | 2 cut(s) | 97, 371 |
| HinfI | GANTC | 1 cut(s) | 410 |
| HpaI | GTTAAC | 1 cut(s) | 97 |
| HpaII | CCGG | 1 cut(s) | 381 |
| HphI | GGTGA | 1 cut(s) | 545 |
| Hpy166II | GTNNAC | 2 cut(s) | 97, 371 |
| Hpy188I | TCNGA | 1 cut(s) | 126 |
| Hpy188III | TCNNGA | 1 cut(s) | 538 |
| Hpy8I | GTNNAC | 2 cut(s) | 97, 371 |
| HpyAV | CCTTC | 2 cut(s) | 68, 217 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 155 |
| HpyCH4IV | ACGT | 2 cut(s) | 99, 364 |
| HpyCH4V | TGCA | 1 cut(s) | 492 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 137, 156, 165, 317, 332, 343, 404, 426, 473 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| HpySE526I | ACGT | 2 cut(s) | 99, 364 |
| Hsp92I | GRCGYC | 1 cut(s) | 502 |
| HspAI | GCGC | 2 cut(s) | 335, 502 |
| KasI | GGCGCC | 1 cut(s) | 501 |
| KspAI | GTTAAC | 1 cut(s) | 97 |
| KspI | CCGCGG | 1 cut(s) | 75 |
| Kzo9I | GATC | 2 cut(s) | 40, 172 |
| LmnI | GCTCC | 2 cut(s) | 354, 395 |
| Lsp1109I | GCAGC | 1 cut(s) | 143 |
| MaeI | CTAG | 2 cut(s) | 453, 559 |
| MaeII | ACGT | 2 cut(s) | 99, 364 |
| MalI | GATC | 2 cut(s) | 42, 174 |
| MbiI | CCGCTC | 1 cut(s) | 527 |
| MboI | GATC | 2 cut(s) | 40, 172 |
| MboII | GAAGA | 1 cut(s) | 232 |
| MflI | RGATCY | 1 cut(s) | 172 |
| MhlI | GDGCHC | 3 cut(s) | 243, 359, 427 |
| MlsI | TGGCCA | 1 cut(s) | 275 |
| MluCI | AATT | 2 cut(s) | 290, 468 |
| MluNI | TGGCCA | 1 cut(s) | 275 |
| Mly113I | GGCGCC | 1 cut(s) | 502 |
| MmeI | TCCRAC | 1 cut(s) | 149 |
| Mox20I | TGGCCA | 1 cut(s) | 275 |
| MscI | TGGCCA | 1 cut(s) | 275 |
| MseI | TTAA | 4 cut(s) | 84, 96, 102, 471 |
| MslI | CAYNNNNRTG | 1 cut(s) | 281 |
| Msp20I | TGGCCA | 1 cut(s) | 275 |
| MspA1I | CMGCKG | 4 cut(s) | 74, 134, 407, 429 |
| MspI | CCGG | 1 cut(s) | 381 |
| MspR9I | CCNGG | 2 cut(s) | 170, 417 |
| MvaI | CCWGG | 2 cut(s) | 170, 417 |
| MvnI | CGCG | 3 cut(s) | 74, 231, 352 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 137, 156, 165, 317, 332, 343, 404, 426, 473 |
| NarI | GGCGCC | 1 cut(s) | 502 |
| NdeII | GATC | 2 cut(s) | 40, 172 |
| NlaIV | GGNNCC | 2 cut(s) | 425, 503 |
| NmeAIII | GCCGAG | 1 cut(s) | 280 |
| PaqCI | CACCTGC | 1 cut(s) | 259 |
| PfeI | GAWTC | 1 cut(s) | 410 |
| PflFI | GACNNNGTC | 1 cut(s) | 366 |
| PkrI | GCNGC | 2 cut(s) | 76, 133 |
| PluTI | GGCGCC | 1 cut(s) | 505 |
| Psp1406I | AACGTT | 1 cut(s) | 99 |
| Psp6I | CCWGG | 2 cut(s) | 168, 415 |
| PspFI | CCCAGC | 1 cut(s) | 331 |
| PspGI | CCWGG | 2 cut(s) | 168, 415 |
| PspN4I | GGNNCC | 2 cut(s) | 425, 503 |
| PspOMI | GGGCCC | 1 cut(s) | 423 |
| PspPI | GGNCC | 3 cut(s) | 259, 423, 424 |
| PstI | CTGCAG | 1 cut(s) | 494 |
| PsuI | RGATCY | 1 cut(s) | 172 |
| PsyI | GACNNNGTC | 1 cut(s) | 366 |
| RseI | CAYNNNNRTG | 1 cut(s) | 281 |
| SacII | CCGCGG | 1 cut(s) | 75 |
| SaqAI | TTAA | 4 cut(s) | 84, 96, 102, 471 |
| SatI | GCNGC | 2 cut(s) | 75, 132 |
| Sau3AI | GATC | 2 cut(s) | 40, 172 |
| Sau96I | GGNCC | 3 cut(s) | 259, 423, 424 |
| ScrFI | CCNGG | 2 cut(s) | 170, 417 |
| SduI | GDGCHC | 3 cut(s) | 243, 359, 427 |
| SetI | ASST | 6 cut(s) | 102, 195, 273, 328, 367, 400 |
| SfcI | CTRYAG | 1 cut(s) | 490 |
| SfoI | GGCGCC | 1 cut(s) | 503 |
| Sfr303I | CCGCGG | 1 cut(s) | 75 |
| SgrBI | CCGCGG | 1 cut(s) | 75 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 281 |
| Sse9I | AATT | 2 cut(s) | 290, 468 |
| SspDI | GGCGCC | 1 cut(s) | 501 |
| SspMI | CTAG | 2 cut(s) | 453, 559 |
| StyD4I | CCNGG | 2 cut(s) | 168, 415 |
| TaaI | ACNGT | 2 cut(s) | 11, 155 |
| TaiI | ACGT | 2 cut(s) | 102, 367 |
| TaqI | TCGA | 1 cut(s) | 106 |
| TasI | AATT | 2 cut(s) | 290, 468 |
| TauI | GCSGC | 1 cut(s) | 77 |
| TfiI | GAWTC | 1 cut(s) | 410 |
| Tru1I | TTAA | 4 cut(s) | 84, 96, 102, 471 |
| Tru9I | TTAA | 4 cut(s) | 84, 96, 102, 471 |
| TscAI | CASTG | 1 cut(s) | 96 |
| TseI | GCWGC | 1 cut(s) | 131 |
| TspDTI | ATGAA | 1 cut(s) | 312 |
| TspRI | CASTG | 1 cut(s) | 96 |
| Tth111I | GACNNNGTC | 1 cut(s) | 366 |
| XapI | RAATTY | 1 cut(s) | 290 |
| XspI | CTAG | 2 cut(s) | 453, 559 |