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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 2 cut(s) | 435, 547 |
| AccII | CGCG | 1 cut(s) | 271 |
| AclI | AACGTT | 1 cut(s) | 78 |
| AclWI | GGATC | 1 cut(s) | 48 |
| AcoI | YGGCCR | 2 cut(s) | 72, 419 |
| AcsI | RAATTY | 1 cut(s) | 209 |
| AcuI | CTGAAG | 2 cut(s) | 48, 242 |
| AdeI | CACNNNGTG | 1 cut(s) | 136 |
| AfaI | GTAC | 1 cut(s) | 312 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 187 |
| AgsI | TTSAA | 1 cut(s) | 346 |
| AluBI | AGCT | 4 cut(s) | 112, 160, 358, 487 |
| AluI | AGCT | 4 cut(s) | 112, 160, 358, 487 |
| Alw21I | GWGCWC | 2 cut(s) | 162, 278 |
| Alw26I | GTCTC | 1 cut(s) | 516 |
| AlwI | GGATC | 1 cut(s) | 48 |
| AoxI | GGCC | 4 cut(s) | 72, 171, 419, 447 |
| ApeKI | GCWGC | 1 cut(s) | 553 |
| ApoI | RAATTY | 1 cut(s) | 209 |
| AspA2I | CCTAGG | 1 cut(s) | 476 |
| AspLEI | GCGC | 1 cut(s) | 232 |
| AspS9I | GGNCC | 4 cut(s) | 91, 371, 447, 512 |
| AsuHPI | GGTGA | 1 cut(s) | 148 |
| AvaII | GGWCC | 3 cut(s) | 91, 371, 512 |
| AvrII | CCTAGG | 1 cut(s) | 476 |
| BalI | TGGCCA | 1 cut(s) | 74 |
| BanII | GRGCYC | 1 cut(s) | 162 |
| Bbv12I | GWGCWC | 2 cut(s) | 162, 278 |
| BccI | CCATC | 1 cut(s) | 512 |
| BceAI | ACGGC | 1 cut(s) | 434 |
| BclI | TGATCA | 1 cut(s) | 151 |
| BcoDI | GTCTC | 1 cut(s) | 516 |
| BfaI | CTAG | 4 cut(s) | 119, 279, 353, 477 |
| BfoI | RGCGCY | 1 cut(s) | 233 |
| BisI | GCNGC | 2 cut(s) | 433, 554 |
| BlnI | CCTAGG | 1 cut(s) | 476 |
| BlsI | GCNGC | 2 cut(s) | 434, 555 |
| Bme18I | GGWCC | 3 cut(s) | 91, 371, 512 |
| BmgT120I | GGNCC | 4 cut(s) | 91, 371, 447, 512 |
| BmiI | GGNNCC | 3 cut(s) | 372, 513, 514 |
| BmsI | GCATC | 3 cut(s) | 321, 510, 540 |
| BpmI | CTGGAG | 1 cut(s) | 563 |
| BsaJI | CCNNGG | 2 cut(s) | 69, 476 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 398, 428 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 187 |
| Bse1I | ACTGG | 1 cut(s) | 546 |
| BseDI | CCNNGG | 2 cut(s) | 69, 476 |
| BseGI | GGATG | 2 cut(s) | 501, 555 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 187 |
| BseMII | CTCAG | 2 cut(s) | 175, 447 |
| BseNI | ACTGG | 1 cut(s) | 546 |
| Bsh1236I | CGCG | 1 cut(s) | 271 |
| BshFI | GGCC | 4 cut(s) | 74, 173, 421, 449 |
| BsiHKAI | GWGCWC | 2 cut(s) | 162, 278 |
| BslFI | GGGAC | 3 cut(s) | 384, 458, 525 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 187 |
| BsmAI | GTCTC | 1 cut(s) | 516 |
| BsmFI | GGGAC | 3 cut(s) | 384, 458, 525 |
| BsnI | GGCC | 4 cut(s) | 74, 173, 421, 449 |
| Bsp1286I | GDGCHC | 2 cut(s) | 162, 278 |
| Bsp143I | GATC | 2 cut(s) | 40, 151 |
| Bsp19I | CCATGG | 1 cut(s) | 69 |
| BspANI | GGCC | 4 cut(s) | 74, 173, 421, 449 |
| BspCNI | CTCAG | 2 cut(s) | 174, 448 |
| BspFNI | CGCG | 1 cut(s) | 271 |
| BspLI | GGNNCC | 3 cut(s) | 372, 513, 514 |
| BspPI | GGATC | 1 cut(s) | 48 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BsrBI | CCGCTC | 2 cut(s) | 435, 547 |
| BsrI | ACTGG | 1 cut(s) | 546 |
| BssECI | CCNNGG | 2 cut(s) | 69, 476 |
| BssMI | GATC | 2 cut(s) | 40, 151 |
| BssT1I | CCWWGG | 2 cut(s) | 69, 476 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 342 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 231 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 114, 430 |
| BstDEI | CTNAG | 2 cut(s) | 161, 456 |
| BstDSI | CCRYGG | 1 cut(s) | 69 |
| BstF5I | GGATG | 2 cut(s) | 501, 555 |
| BstFNI | CGCG | 1 cut(s) | 271 |
| BstH2I | RGCGCY | 1 cut(s) | 233 |
| BstHHI | GCGC | 1 cut(s) | 232 |
| BstKTI | GATC | 2 cut(s) | 43, 154 |
| BstMAI | GTCTC | 1 cut(s) | 516 |
| BstMBI | GATC | 2 cut(s) | 40, 151 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 95, 262, 282, 493, 553 |
| BstNSI | RCATGY | 1 cut(s) | 428 |
| BstUI | CGCG | 1 cut(s) | 271 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 70, 307, 541 |
| BsuRI | GGCC | 4 cut(s) | 74, 173, 421, 449 |
| BtgI | CCRYGG | 1 cut(s) | 69 |
| BtsCI | GGATG | 2 cut(s) | 501, 555 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 114, 430 |
| CfoI | GCGC | 1 cut(s) | 232 |
| Cfr13I | GGNCC | 4 cut(s) | 91, 371, 447, 512 |
| Csp6I | GTAC | 1 cut(s) | 311 |
| CviAII | CATG | 2 cut(s) | 70, 425 |
| CviQI | GTAC | 1 cut(s) | 311 |
| DdeI | CTNAG | 2 cut(s) | 161, 456 |
| DpnI | GATC | 2 cut(s) | 42, 153 |
| DpnII | GATC | 2 cut(s) | 40, 151 |
| DraIII | CACNNNGTG | 1 cut(s) | 136 |
| EaeI | YGGCCR | 2 cut(s) | 72, 419 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 231 |
| EarI | CTCTTC | 2 cut(s) | 24, 231 |
| EciI | GGCGGA | 1 cut(s) | 53 |
| Ecl136II | GAGCTC | 1 cut(s) | 160 |
| Eco130I | CCWWGG | 2 cut(s) | 69, 476 |
| Eco24I | GRGCYC | 1 cut(s) | 162 |
| Eco47I | GGWCC | 3 cut(s) | 91, 371, 512 |
| Eco53kI | GAGCTC | 1 cut(s) | 160 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 242 |
| EcoICRI | GAGCTC | 1 cut(s) | 160 |
| EcoO109I | RGGNCCY | 1 cut(s) | 512 |
| EcoT14I | CCWWGG | 2 cut(s) | 69, 476 |
| EcoT38I | GRGCYC | 1 cut(s) | 162 |
| ErhI | CCWWGG | 2 cut(s) | 69, 476 |
| FaeI | CATG | 2 cut(s) | 73, 428 |
| FaiI | YATR | 8 cut(s) | 71, 104, 108, 326, 361, 426, 467, 536 |
| FaqI | GGGAC | 3 cut(s) | 384, 458, 525 |
| FatI | CATG | 2 cut(s) | 69, 424 |
| FauI | CCCGC | 2 cut(s) | 8, 489 |
| FbaI | TGATCA | 1 cut(s) | 151 |
| Fnu4HI | GCNGC | 2 cut(s) | 433, 554 |
| FokI | GGATG | 2 cut(s) | 488, 562 |
| FriOI | GRGCYC | 1 cut(s) | 162 |
| Fsp4HI | GCNGC | 2 cut(s) | 433, 554 |
| FspBI | CTAG | 4 cut(s) | 119, 279, 353, 477 |
| GlaI | GCGC | 1 cut(s) | 231 |
| GluI | GCNGC | 2 cut(s) | 433, 554 |
| GsuI | CTGGAG | 1 cut(s) | 563 |
| HaeII | RGCGCY | 1 cut(s) | 233 |
| HaeIII | GGCC | 4 cut(s) | 74, 173, 421, 449 |
| HhaI | GCGC | 1 cut(s) | 232 |
| Hin1II | CATG | 2 cut(s) | 73, 428 |
| Hin6I | GCGC | 1 cut(s) | 230 |
| HinP1I | GCGC | 1 cut(s) | 230 |
| HincII | GTYRAC | 1 cut(s) | 290 |
| HindII | GTYRAC | 1 cut(s) | 290 |
| HinfI | GANTC | 1 cut(s) | 413 |
| HphI | GGTGA | 1 cut(s) | 148 |
| Hpy166II | GTNNAC | 2 cut(s) | 290, 313 |
| Hpy188I | TCNGA | 1 cut(s) | 164 |
| Hpy188III | TCNNGA | 3 cut(s) | 155, 380, 505 |
| Hpy8I | GTNNAC | 2 cut(s) | 290, 313 |
| HpyAV | CCTTC | 2 cut(s) | 220, 502 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 342 |
| HpyCH4IV | ACGT | 1 cut(s) | 78 |
| HpyCH4V | TGCA | 2 cut(s) | 334, 553 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 95, 262, 282, 493, 553 |
| HpyF3I | CTNAG | 2 cut(s) | 161, 456 |
| HpySE526I | ACGT | 1 cut(s) | 78 |
| Hsp92II | CATG | 2 cut(s) | 73, 428 |
| HspAI | GCGC | 1 cut(s) | 230 |
| KflI | GGGWCCC | 1 cut(s) | 512 |
| Ksp22I | TGATCA | 1 cut(s) | 151 |
| Kzo9I | GATC | 2 cut(s) | 40, 151 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 6 cut(s) | 157, 262, 273, 282, 400, 544 |
| LweI | GCATC | 3 cut(s) | 321, 510, 540 |
| MaeI | CTAG | 4 cut(s) | 119, 279, 353, 477 |
| MaeII | ACGT | 1 cut(s) | 78 |
| MaeIII | GTNAC | 1 cut(s) | 414 |
| MalI | GATC | 2 cut(s) | 42, 153 |
| MbiI | CCGCTC | 2 cut(s) | 435, 547 |
| MboI | GATC | 2 cut(s) | 40, 151 |
| MboII | GAAGA | 3 cut(s) | 41, 235, 248 |
| MhlI | GDGCHC | 2 cut(s) | 162, 278 |
| MlsI | TGGCCA | 1 cut(s) | 74 |
| MluCI | AATT | 2 cut(s) | 209, 557 |
| MluNI | TGGCCA | 1 cut(s) | 74 |
| MlyI | GAGTC | 1 cut(s) | 422 |
| MmeI | TCCRAC | 1 cut(s) | 378 |
| MnlI | CCTC | 4 cut(s) | 104, 387, 400, 451 |
| Mox20I | TGGCCA | 1 cut(s) | 74 |
| MscI | TGGCCA | 1 cut(s) | 74 |
| MseI | TTAA | 1 cut(s) | 81 |
| MslI | CAYNNNNRTG | 2 cut(s) | 200, 329 |
| Msp20I | TGGCCA | 1 cut(s) | 74 |
| MspA1I | CMGCKG | 3 cut(s) | 89, 303, 496 |
| MvnI | CGCG | 1 cut(s) | 271 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 95, 262, 282, 493, 553 |
| NcoI | CCATGG | 1 cut(s) | 69 |
| NdeII | GATC | 2 cut(s) | 40, 151 |
| NlaIII | CATG | 2 cut(s) | 73, 428 |
| NlaIV | GGNNCC | 3 cut(s) | 372, 513, 514 |
| NmuCI | GTSAC | 1 cut(s) | 414 |
| NspI | RCATGY | 1 cut(s) | 428 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PkrI | GCNGC | 2 cut(s) | 434, 555 |
| PleI | GAGTC | 1 cut(s) | 421 |
| PpsI | GAGTC | 1 cut(s) | 421 |
| PpuMI | RGGWCCY | 1 cut(s) | 512 |
| Psp124BI | GAGCTC | 1 cut(s) | 162 |
| Psp1406I | AACGTT | 1 cut(s) | 78 |
| Psp5II | RGGWCCY | 1 cut(s) | 512 |
| PspN4I | GGNNCC | 3 cut(s) | 372, 513, 514 |
| PspPI | GGNCC | 4 cut(s) | 91, 371, 447, 512 |
| PspPPI | RGGWCCY | 1 cut(s) | 512 |
| RsaI | GTAC | 1 cut(s) | 312 |
| RsaNI | GTAC | 1 cut(s) | 311 |
| RseI | CAYNNNNRTG | 2 cut(s) | 200, 329 |
| SacI | GAGCTC | 1 cut(s) | 162 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 81 |
| SatI | GCNGC | 2 cut(s) | 433, 554 |
| Sau3AI | GATC | 2 cut(s) | 40, 151 |
| Sau96I | GGNCC | 4 cut(s) | 91, 371, 447, 512 |
| SchI | GAGTC | 1 cut(s) | 422 |
| SduI | GDGCHC | 2 cut(s) | 162, 278 |
| SetI | ASST | 9 cut(s) | 81, 96, 114, 138, 162, 192, 360, 379, 489 |
| SfaNI | GCATC | 3 cut(s) | 321, 510, 540 |
| SinI | GGWCC | 3 cut(s) | 91, 371, 512 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 200, 329 |
| Sse9I | AATT | 2 cut(s) | 209, 557 |
| SspMI | CTAG | 4 cut(s) | 119, 279, 353, 477 |
| SstI | GAGCTC | 1 cut(s) | 162 |
| StyI | CCWWGG | 2 cut(s) | 69, 476 |
| TaaI | ACNGT | 2 cut(s) | 11, 342 |
| TaiI | ACGT | 1 cut(s) | 81 |
| TasI | AATT | 2 cut(s) | 209, 557 |
| TauI | GCSGC | 1 cut(s) | 435 |
| Tru1I | TTAA | 1 cut(s) | 81 |
| Tru9I | TTAA | 1 cut(s) | 81 |
| TseFI | GTSAC | 1 cut(s) | 414 |
| TseI | GCWGC | 1 cut(s) | 553 |
| Tsp45I | GTSAC | 1 cut(s) | 414 |
| VpaK11BI | GGWCC | 3 cut(s) | 91, 371, 512 |
| XapI | RAATTY | 1 cut(s) | 209 |
| XceI | RCATGY | 1 cut(s) | 428 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 304, 357 |
| XmaJI | CCTAGG | 1 cut(s) | 476 |
| XspI | CTAG | 4 cut(s) | 119, 279, 353, 477 |