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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 241 |
| Acc36I | ACCTGC | 1 cut(s) | 241 |
| AccBSI | CCGCTC | 1 cut(s) | 100 |
| AccII | CGCG | 1 cut(s) | 61 |
| AciI | CCGC | 7 cut(s) | 38, 46, 49, 59, 98, 116, 185 |
| AclWI | GGATC | 2 cut(s) | 37, 195 |
| AcoI | YGGCCR | 1 cut(s) | 255 |
| AcuI | CTGAAG | 1 cut(s) | 99 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 250 |
| AluBI | AGCT | 3 cut(s) | 22, 92, 175 |
| AluI | AGCT | 3 cut(s) | 22, 92, 175 |
| Alw26I | GTCTC | 1 cut(s) | 113 |
| AlwI | GGATC | 2 cut(s) | 37, 195 |
| AoxI | GGCC | 3 cut(s) | 234, 240, 255 |
| AspLEI | GCGC | 3 cut(s) | 58, 63, 225 |
| AspS9I | GGNCC | 1 cut(s) | 241 |
| AsuC2I | CCSGG | 1 cut(s) | 129 |
| AsuHPI | GGTGA | 3 cut(s) | 16, 27, 62 |
| BalI | TGGCCA | 1 cut(s) | 257 |
| BanII | GRGCYC | 1 cut(s) | 18 |
| BcnI | CCSGG | 1 cut(s) | 129 |
| BcoDI | GTCTC | 1 cut(s) | 113 |
| BfmI | CTRYAG | 1 cut(s) | 111 |
| BfuAI | ACCTGC | 1 cut(s) | 241 |
| BisI | GCNGC | 2 cut(s) | 49, 59 |
| BlsI | GCNGC | 2 cut(s) | 50, 60 |
| Bme1390I | CCNGG | 1 cut(s) | 129 |
| BmgT120I | GGNCC | 1 cut(s) | 241 |
| BmrFI | CCNGG | 1 cut(s) | 129 |
| BmsI | GCATC | 1 cut(s) | 92 |
| BpuMI | CCSGG | 1 cut(s) | 129 |
| BsaJI | CCNNGG | 1 cut(s) | 237 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 250 |
| Bse1I | ACTGG | 2 cut(s) | 151, 186 |
| BseDI | CCNNGG | 1 cut(s) | 237 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 250 |
| BseNI | ACTGG | 2 cut(s) | 151, 186 |
| BseYI | CCCAGC | 2 cut(s) | 18, 313 |
| Bsh1236I | CGCG | 1 cut(s) | 61 |
| Bsh1285I | CGRYCG | 1 cut(s) | 128 |
| BshFI | GGCC | 3 cut(s) | 236, 242, 257 |
| BsiEI | CGRYCG | 1 cut(s) | 128 |
| BsiSI | CCGG | 1 cut(s) | 128 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 250 |
| BsmAI | GTCTC | 1 cut(s) | 113 |
| BsnI | GGCC | 3 cut(s) | 236, 242, 257 |
| Bsp1286I | GDGCHC | 1 cut(s) | 18 |
| Bsp143I | GATC | 4 cut(s) | 32, 42, 67, 187 |
| BspACI | CCGC | 7 cut(s) | 38, 46, 49, 59, 98, 116, 185 |
| BspANI | GGCC | 3 cut(s) | 236, 242, 257 |
| BspFNI | CGCG | 1 cut(s) | 61 |
| BspMI | ACCTGC | 1 cut(s) | 241 |
| BspPI | GGATC | 2 cut(s) | 37, 195 |
| BsrBI | CCGCTC | 1 cut(s) | 100 |
| BsrI | ACTGG | 2 cut(s) | 151, 186 |
| BssECI | CCNNGG | 1 cut(s) | 237 |
| BssMI | GATC | 4 cut(s) | 32, 42, 67, 187 |
| BssT1I | CCWWGG | 1 cut(s) | 237 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 138 |
| BstC8I | GCNNGC | 2 cut(s) | 177, 202 |
| BstFNI | CGCG | 1 cut(s) | 61 |
| BstHHI | GCGC | 3 cut(s) | 58, 63, 225 |
| BstKTI | GATC | 4 cut(s) | 35, 45, 70, 190 |
| BstMAI | GTCTC | 1 cut(s) | 113 |
| BstMBI | GATC | 4 cut(s) | 32, 42, 67, 187 |
| BstMCI | CGRYCG | 1 cut(s) | 128 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 89, 138, 314 |
| BstSCI | CCNGG | 1 cut(s) | 127 |
| BstSFI | CTRYAG | 1 cut(s) | 111 |
| BstUI | CGCG | 1 cut(s) | 61 |
| BstX2I | RGATCY | 1 cut(s) | 187 |
| BstYI | RGATCY | 1 cut(s) | 187 |
| BsuRI | GGCC | 3 cut(s) | 236, 242, 257 |
| BtsIMutI | CAGTG | 1 cut(s) | 179 |
| BveI | ACCTGC | 1 cut(s) | 241 |
| Cac8I | GCNNGC | 2 cut(s) | 177, 202 |
| CfoI | GCGC | 3 cut(s) | 58, 63, 225 |
| Cfr13I | GGNCC | 1 cut(s) | 241 |
| CviAII | CATG | 2 cut(s) | 11, 53 |
| CviJI | RGCY | 7 cut(s) | 16, 22, 92, 175, 236, 242, 257 |
| CviKI_1 | RGCY | 7 cut(s) | 16, 22, 92, 175, 236, 242, 257 |
| DpnI | GATC | 4 cut(s) | 34, 44, 69, 189 |
| DpnII | GATC | 4 cut(s) | 32, 42, 67, 187 |
| EaeI | YGGCCR | 1 cut(s) | 255 |
| EciI | GGCGGA | 2 cut(s) | 35, 200 |
| Eco130I | CCWWGG | 1 cut(s) | 237 |
| Eco24I | GRGCYC | 1 cut(s) | 18 |
| Eco57I | CTGAAG | 1 cut(s) | 99 |
| EcoT14I | CCWWGG | 1 cut(s) | 237 |
| EcoT38I | GRGCYC | 1 cut(s) | 18 |
| ErhI | CCWWGG | 1 cut(s) | 237 |
| FaeI | CATG | 2 cut(s) | 14, 56 |
| FaiI | YATR | 4 cut(s) | 12, 54, 167, 171 |
| FatI | CATG | 2 cut(s) | 10, 52 |
| FauI | CCCGC | 1 cut(s) | 105 |
| Fnu4HI | GCNGC | 2 cut(s) | 49, 59 |
| FriOI | GRGCYC | 1 cut(s) | 18 |
| Fsp4HI | GCNGC | 2 cut(s) | 49, 59 |
| GlaI | GCGC | 3 cut(s) | 57, 62, 224 |
| GluI | GCNGC | 2 cut(s) | 49, 59 |
| GsaI | CCCAGC | 2 cut(s) | 22, 317 |
| HaeIII | GGCC | 3 cut(s) | 236, 242, 257 |
| HapII | CCGG | 1 cut(s) | 128 |
| HhaI | GCGC | 3 cut(s) | 58, 63, 225 |
| Hin1II | CATG | 2 cut(s) | 14, 56 |
| Hin6I | GCGC | 3 cut(s) | 56, 61, 223 |
| HinP1I | GCGC | 3 cut(s) | 56, 61, 223 |
| HindIII | AAGCTT | 1 cut(s) | 90 |
| HinfI | GANTC | 1 cut(s) | 121 |
| HpaII | CCGG | 1 cut(s) | 128 |
| HphI | GGTGA | 3 cut(s) | 16, 27, 62 |
| Hpy166II | GTNNAC | 1 cut(s) | 215 |
| Hpy188I | TCNGA | 1 cut(s) | 88 |
| Hpy188III | TCNNGA | 1 cut(s) | 152 |
| Hpy8I | GTNNAC | 1 cut(s) | 215 |
| HpyAV | CCTTC | 1 cut(s) | 226 |
| HpyCH4IV | ACGT | 1 cut(s) | 291 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 89, 138, 314 |
| HpySE526I | ACGT | 1 cut(s) | 291 |
| Hsp92II | CATG | 2 cut(s) | 14, 56 |
| HspAI | GCGC | 3 cut(s) | 56, 61, 223 |
| Kzo9I | GATC | 4 cut(s) | 32, 42, 67, 187 |
| LpnPI | CCDG | 9 cut(s) | 32, 43, 132, 141, 161, 167, 236, 281, 327 |
| LweI | GCATC | 1 cut(s) | 92 |
| MaeII | ACGT | 1 cut(s) | 291 |
| MalI | GATC | 4 cut(s) | 34, 44, 69, 189 |
| MbiI | CCGCTC | 1 cut(s) | 100 |
| MboI | GATC | 4 cut(s) | 32, 42, 67, 187 |
| MfeI | CAATTG | 1 cut(s) | 136 |
| MflI | RGATCY | 1 cut(s) | 187 |
| MhlI | GDGCHC | 1 cut(s) | 18 |
| MlsI | TGGCCA | 1 cut(s) | 257 |
| MluCI | AATT | 1 cut(s) | 136 |
| MluNI | TGGCCA | 1 cut(s) | 257 |
| MlyI | GAGTC | 1 cut(s) | 115 |
| MnlI | CCTC | 3 cut(s) | 83, 172, 264 |
| Mox20I | TGGCCA | 1 cut(s) | 257 |
| MscI | TGGCCA | 1 cut(s) | 257 |
| MslI | CAYNNNNRTG | 1 cut(s) | 263 |
| Msp20I | TGGCCA | 1 cut(s) | 257 |
| MspA1I | CMGCKG | 2 cut(s) | 40, 116 |
| MspI | CCGG | 1 cut(s) | 128 |
| MspR9I | CCNGG | 1 cut(s) | 129 |
| MteI | GCGCNGCGC | 1 cut(s) | 59 |
| MunI | CAATTG | 1 cut(s) | 136 |
| MvnI | CGCG | 1 cut(s) | 61 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 89, 138, 314 |
| NciI | CCSGG | 1 cut(s) | 129 |
| NdeII | GATC | 4 cut(s) | 32, 42, 67, 187 |
| NlaIII | CATG | 2 cut(s) | 14, 56 |
| PaqCI | CACCTGC | 1 cut(s) | 241 |
| PkrI | GCNGC | 2 cut(s) | 50, 60 |
| PleI | GAGTC | 1 cut(s) | 115 |
| PpsI | GAGTC | 1 cut(s) | 115 |
| PspFI | CCCAGC | 2 cut(s) | 18, 313 |
| PspPI | GGNCC | 1 cut(s) | 241 |
| PsuI | RGATCY | 1 cut(s) | 187 |
| RseI | CAYNNNNRTG | 1 cut(s) | 263 |
| SatI | GCNGC | 2 cut(s) | 49, 59 |
| Sau3AI | GATC | 4 cut(s) | 32, 42, 67, 187 |
| Sau96I | GGNCC | 1 cut(s) | 241 |
| SchI | GAGTC | 1 cut(s) | 115 |
| ScrFI | CCNGG | 1 cut(s) | 129 |
| SduI | GDGCHC | 1 cut(s) | 18 |
| SetI | ASST | 8 cut(s) | 24, 75, 94, 159, 177, 255, 275, 294 |
| SfaNI | GCATC | 1 cut(s) | 92 |
| SfcI | CTRYAG | 1 cut(s) | 111 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 263 |
| Sse9I | AATT | 1 cut(s) | 136 |
| SsiI | CCGC | 7 cut(s) | 38, 46, 49, 59, 98, 116, 185 |
| StyD4I | CCNGG | 1 cut(s) | 127 |
| StyI | CCWWGG | 1 cut(s) | 237 |
| TaiI | ACGT | 1 cut(s) | 294 |
| TaqI | TCGA | 3 cut(s) | 124, 269, 276 |
| TasI | AATT | 1 cut(s) | 136 |
| TauI | GCSGC | 2 cut(s) | 51, 61 |
| TscAI | CASTG | 1 cut(s) | 186 |
| TspRI | CASTG | 1 cut(s) | 186 |