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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 310 |
| Acc36I | ACCTGC | 1 cut(s) | 310 |
| AccBSI | CCGCTC | 2 cut(s) | 201, 425 |
| AclI | AACGTT | 2 cut(s) | 81, 99 |
| AclWI | GGATC | 2 cut(s) | 35, 48 |
| AcoI | YGGCCR | 1 cut(s) | 324 |
| AcsI | RAATTY | 1 cut(s) | 341 |
| AfaI | GTAC | 1 cut(s) | 421 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 319, 479, 532 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AjnI | CCWGG | 2 cut(s) | 525, 558 |
| AluBI | AGCT | 3 cut(s) | 244, 440, 569 |
| AluI | AGCT | 3 cut(s) | 244, 440, 569 |
| AlwI | GGATC | 2 cut(s) | 35, 48 |
| Ama87I | CYCGRG | 1 cut(s) | 435 |
| AoxI | GGCC | 5 cut(s) | 150, 309, 324, 452, 571 |
| ApaI | GGGCCC | 1 cut(s) | 456 |
| ApoI | RAATTY | 1 cut(s) | 341 |
| AspLEI | GCGC | 3 cut(s) | 294, 364, 388 |
| AspS9I | GGNCC | 5 cut(s) | 194, 310, 452, 453, 535 |
| AvaI | CYCGRG | 1 cut(s) | 435 |
| AvaII | GGWCC | 2 cut(s) | 194, 535 |
| AxyI | CCTNAGG | 1 cut(s) | 147 |
| BaeGI | GKGCMC | 1 cut(s) | 456 |
| BalI | TGGCCA | 1 cut(s) | 326 |
| BamHI | GGATCC | 1 cut(s) | 40 |
| BanII | GRGCYC | 1 cut(s) | 456 |
| BccI | CCATC | 2 cut(s) | 52, 66 |
| BceAI | ACGGC | 1 cut(s) | 215 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 495, 529 |
| BciT130I | CCWGG | 2 cut(s) | 527, 560 |
| BfaI | CTAG | 3 cut(s) | 251, 575, 592 |
| BfmI | CTRYAG | 1 cut(s) | 441 |
| BfoI | RGCGCY | 1 cut(s) | 365 |
| BfuAI | ACCTGC | 1 cut(s) | 310 |
| BisI | GCNGC | 2 cut(s) | 208, 504 |
| BlsI | GCNGC | 2 cut(s) | 209, 505 |
| Bme1390I | CCNGG | 2 cut(s) | 527, 560 |
| Bme18I | GGWCC | 2 cut(s) | 194, 535 |
| BmeT110I | CYCGRG | 1 cut(s) | 435 |
| BmgT120I | GGNCC | 5 cut(s) | 194, 310, 452, 453, 535 |
| BmiI | GGNNCC | 2 cut(s) | 42, 454 |
| BmrFI | CCNGG | 2 cut(s) | 527, 560 |
| BmsI | GCATC | 1 cut(s) | 207 |
| BpmI | CTGGAG | 2 cut(s) | 386, 501 |
| BsaJI | CCNNGG | 3 cut(s) | 526, 538, 558 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 319, 479, 532 |
| Bse1I | ACTGG | 1 cut(s) | 125 |
| Bse21I | CCTNAGG | 1 cut(s) | 147 |
| BseBI | CCWGG | 2 cut(s) | 527, 560 |
| BseDI | CCNNGG | 3 cut(s) | 526, 538, 558 |
| BseGI | GGATG | 3 cut(s) | 44, 58, 155 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 319, 479, 532 |
| BseMII | CTCAG | 2 cut(s) | 18, 161 |
| BseNI | ACTGG | 1 cut(s) | 125 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseSI | GKGCMC | 1 cut(s) | 456 |
| BseYI | CCCAGC | 2 cut(s) | 134, 382 |
| BshFI | GGCC | 5 cut(s) | 152, 311, 326, 454, 573 |
| BsiHKCI | CYCGRG | 1 cut(s) | 435 |
| BslFI | GGGAC | 1 cut(s) | 452 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 319, 479, 532 |
| BsmFI | GGGAC | 1 cut(s) | 452 |
| BsnI | GGCC | 5 cut(s) | 152, 311, 326, 454, 573 |
| BsoBI | CYCGRG | 1 cut(s) | 435 |
| Bsp120I | GGGCCC | 1 cut(s) | 452 |
| Bsp1286I | GDGCHC | 1 cut(s) | 456 |
| Bsp143I | GATC | 2 cut(s) | 40, 75 |
| BspANI | GGCC | 5 cut(s) | 152, 311, 326, 454, 573 |
| BspCNI | CTCAG | 2 cut(s) | 19, 160 |
| BspLI | GGNNCC | 2 cut(s) | 42, 454 |
| BspMI | ACCTGC | 1 cut(s) | 310 |
| BspPI | GGATC | 2 cut(s) | 35, 48 |
| BsrBI | CCGCTC | 2 cut(s) | 201, 425 |
| BsrI | ACTGG | 1 cut(s) | 125 |
| BssECI | CCNNGG | 3 cut(s) | 526, 538, 558 |
| BssMI | GATC | 2 cut(s) | 40, 75 |
| Bst2UI | CCWGG | 2 cut(s) | 527, 560 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstC8I | GCNNGC | 2 cut(s) | 36, 571 |
| BstDEI | CTNAG | 2 cut(s) | 27, 147 |
| BstF5I | GGATG | 3 cut(s) | 44, 58, 155 |
| BstH2I | RGCGCY | 1 cut(s) | 365 |
| BstHHI | GCGC | 3 cut(s) | 294, 364, 388 |
| BstKTI | GATC | 2 cut(s) | 43, 78 |
| BstMBI | GATC | 2 cut(s) | 40, 75 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 207, 368, 383 |
| BstNI | CCWGG | 2 cut(s) | 527, 560 |
| BstSCI | CCNGG | 2 cut(s) | 525, 558 |
| BstSFI | CTRYAG | 1 cut(s) | 441 |
| BstSLI | GKGCMC | 1 cut(s) | 456 |
| BstX2I | RGATCY | 1 cut(s) | 40 |
| BstYI | RGATCY | 1 cut(s) | 40 |
| Bsu36I | CCTNAGG | 1 cut(s) | 147 |
| BsuRI | GGCC | 5 cut(s) | 152, 311, 326, 454, 573 |
| BtsCI | GGATG | 3 cut(s) | 44, 58, 155 |
| BtsIMutI | CAGTG | 1 cut(s) | 24 |
| BveI | ACCTGC | 1 cut(s) | 310 |
| Cac8I | GCNNGC | 2 cut(s) | 36, 571 |
| CfoI | GCGC | 3 cut(s) | 294, 364, 388 |
| Cfr13I | GGNCC | 5 cut(s) | 194, 310, 452, 453, 535 |
| Csp6I | GTAC | 1 cut(s) | 420 |
| CviAII | CATG | 2 cut(s) | 107, 449 |
| CviQI | GTAC | 1 cut(s) | 420 |
| DdeI | CTNAG | 2 cut(s) | 27, 147 |
| DpnI | GATC | 2 cut(s) | 42, 77 |
| DpnII | GATC | 2 cut(s) | 40, 75 |
| EaeI | YGGCCR | 1 cut(s) | 324 |
| EciI | GGCGGA | 1 cut(s) | 37 |
| Eco24I | GRGCYC | 1 cut(s) | 456 |
| Eco47I | GGWCC | 2 cut(s) | 194, 535 |
| Eco81I | CCTNAGG | 1 cut(s) | 147 |
| Eco88I | CYCGRG | 1 cut(s) | 435 |
| EcoRII | CCWGG | 2 cut(s) | 525, 558 |
| EcoT38I | GRGCYC | 1 cut(s) | 456 |
| FaeI | CATG | 2 cut(s) | 110, 452 |
| FaiI | YATR | 5 cut(s) | 108, 122, 236, 240, 450 |
| FaqI | GGGAC | 1 cut(s) | 452 |
| FatI | CATG | 2 cut(s) | 106, 448 |
| FauI | CCCGC | 2 cut(s) | 8, 174 |
| Fnu4HI | GCNGC | 2 cut(s) | 208, 504 |
| FokI | GGATG | 3 cut(s) | 31, 45, 142 |
| FriOI | GRGCYC | 1 cut(s) | 456 |
| Fsp4HI | GCNGC | 2 cut(s) | 208, 504 |
| FspBI | CTAG | 3 cut(s) | 251, 575, 592 |
| GlaI | GCGC | 3 cut(s) | 293, 363, 387 |
| GluI | GCNGC | 2 cut(s) | 208, 504 |
| GsaI | CCCAGC | 2 cut(s) | 138, 386 |
| GsuI | CTGGAG | 2 cut(s) | 386, 501 |
| HaeII | RGCGCY | 1 cut(s) | 365 |
| HaeIII | GGCC | 5 cut(s) | 152, 311, 326, 454, 573 |
| HhaI | GCGC | 3 cut(s) | 294, 364, 388 |
| Hin1II | CATG | 2 cut(s) | 110, 452 |
| Hin6I | GCGC | 3 cut(s) | 292, 362, 386 |
| HinP1I | GCGC | 3 cut(s) | 292, 362, 386 |
| HincII | GTYRAC | 2 cut(s) | 97, 130 |
| HindII | GTYRAC | 2 cut(s) | 97, 130 |
| HinfI | GANTC | 1 cut(s) | 495 |
| HpaI | GTTAAC | 1 cut(s) | 97 |
| Hpy166II | GTNNAC | 2 cut(s) | 97, 130 |
| Hpy188III | TCNNGA | 1 cut(s) | 435 |
| Hpy8I | GTNNAC | 2 cut(s) | 97, 130 |
| Hpy99I | CGWCG | 1 cut(s) | 350 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 3 cut(s) | 81, 99, 179 |
| HpyCH4V | TGCA | 3 cut(s) | 110, 175, 220 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 207, 368, 383 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 147 |
| HpySE526I | ACGT | 3 cut(s) | 81, 99, 179 |
| Hsp92II | CATG | 2 cut(s) | 110, 452 |
| HspAI | GCGC | 3 cut(s) | 292, 362, 386 |
| KspAI | GTTAAC | 1 cut(s) | 97 |
| Kzo9I | GATC | 2 cut(s) | 40, 75 |
| LmnI | GCTCC | 3 cut(s) | 31, 289, 482 |
| LweI | GCATC | 1 cut(s) | 207 |
| MaeI | CTAG | 3 cut(s) | 251, 575, 592 |
| MaeII | ACGT | 3 cut(s) | 81, 99, 179 |
| MaeIII | GTNAC | 1 cut(s) | 496 |
| MalI | GATC | 2 cut(s) | 42, 77 |
| MbiI | CCGCTC | 2 cut(s) | 201, 425 |
| MboI | GATC | 2 cut(s) | 40, 75 |
| MflI | RGATCY | 1 cut(s) | 40 |
| MhlI | GDGCHC | 1 cut(s) | 456 |
| MlsI | TGGCCA | 1 cut(s) | 326 |
| MluCI | AATT | 2 cut(s) | 341, 507 |
| MluNI | TGGCCA | 1 cut(s) | 326 |
| MlyI | GAGTC | 1 cut(s) | 504 |
| Mox20I | TGGCCA | 1 cut(s) | 326 |
| MscI | TGGCCA | 1 cut(s) | 326 |
| MseI | TTAA | 2 cut(s) | 84, 96 |
| MslI | CAYNNNNRTG | 1 cut(s) | 332 |
| Msp20I | TGGCCA | 1 cut(s) | 326 |
| MspA1I | CMGCKG | 1 cut(s) | 185 |
| MspR9I | CCNGG | 2 cut(s) | 527, 560 |
| MvaI | CCWGG | 2 cut(s) | 527, 560 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 207, 368, 383 |
| NdeII | GATC | 2 cut(s) | 40, 75 |
| NlaIII | CATG | 2 cut(s) | 110, 452 |
| NlaIV | GGNNCC | 2 cut(s) | 42, 454 |
| NmeAIII | GCCGAG | 1 cut(s) | 164 |
| NmuCI | GTSAC | 1 cut(s) | 496 |
| PaqCI | CACCTGC | 1 cut(s) | 310 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 87 |
| PkrI | GCNGC | 2 cut(s) | 209, 505 |
| PleI | GAGTC | 1 cut(s) | 503 |
| PpsI | GAGTC | 1 cut(s) | 503 |
| Psp1406I | AACGTT | 2 cut(s) | 81, 99 |
| Psp6I | CCWGG | 2 cut(s) | 525, 558 |
| PspFI | CCCAGC | 2 cut(s) | 134, 382 |
| PspGI | CCWGG | 2 cut(s) | 525, 558 |
| PspN4I | GGNNCC | 2 cut(s) | 42, 454 |
| PspOMI | GGGCCC | 1 cut(s) | 452 |
| PspPI | GGNCC | 5 cut(s) | 194, 310, 452, 453, 535 |
| PsuI | RGATCY | 1 cut(s) | 40 |
| RsaI | GTAC | 1 cut(s) | 421 |
| RsaNI | GTAC | 1 cut(s) | 420 |
| RseI | CAYNNNNRTG | 1 cut(s) | 332 |
| SaqAI | TTAA | 2 cut(s) | 84, 96 |
| SatI | GCNGC | 2 cut(s) | 208, 504 |
| Sau3AI | GATC | 2 cut(s) | 40, 75 |
| Sau96I | GGNCC | 5 cut(s) | 194, 310, 452, 453, 535 |
| SchI | GAGTC | 1 cut(s) | 504 |
| ScrFI | CCNGG | 2 cut(s) | 527, 560 |
| SduI | GDGCHC | 1 cut(s) | 456 |
| SfaNI | GCATC | 1 cut(s) | 207 |
| SfcI | CTRYAG | 1 cut(s) | 441 |
| SinI | GGWCC | 2 cut(s) | 194, 535 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 332 |
| Sse9I | AATT | 2 cut(s) | 341, 507 |
| SspMI | CTAG | 3 cut(s) | 251, 575, 592 |
| StyD4I | CCNGG | 2 cut(s) | 525, 558 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 3 cut(s) | 84, 102, 182 |
| TaqI | TCGA | 1 cut(s) | 345 |
| TaqII | GACCGA | 2 cut(s) | 211, 552 |
| TasI | AATT | 2 cut(s) | 341, 507 |
| TauI | GCSGC | 2 cut(s) | 210, 506 |
| Tru1I | TTAA | 2 cut(s) | 84, 96 |
| Tru9I | TTAA | 2 cut(s) | 84, 96 |
| TscAI | CASTG | 1 cut(s) | 31 |
| TseFI | GTSAC | 1 cut(s) | 496 |
| Tsp45I | GTSAC | 1 cut(s) | 496 |
| TspRI | CASTG | 1 cut(s) | 31 |
| VpaK11BI | GGWCC | 2 cut(s) | 194, 535 |
| XapI | RAATTY | 1 cut(s) | 341 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 566 |
| XspI | CTAG | 3 cut(s) | 251, 575, 592 |