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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 635 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 91 |
| AccII | CGCG | 2 cut(s) | 18, 471 |
| AciI | CCGC | 5 cut(s) | 16, 77, 386, 419, 632 |
| AclWI | GGATC | 1 cut(s) | 177 |
| AcoI | YGGCCR | 1 cut(s) | 130 |
| AcsI | RAATTY | 2 cut(s) | 254, 549 |
| AcyI | GRCGYC | 1 cut(s) | 570 |
| AfaI | GTAC | 1 cut(s) | 384 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 91, 484 |
| AgsI | TTSAA | 2 cut(s) | 28, 53 |
| AhlI | ACTAGT | 1 cut(s) | 163 |
| AjnI | CCWGG | 1 cut(s) | 132 |
| AluBI | AGCT | 3 cut(s) | 157, 328, 403 |
| AluI | AGCT | 3 cut(s) | 157, 328, 403 |
| Alw26I | GTCTC | 1 cut(s) | 122 |
| AlwI | GGATC | 1 cut(s) | 177 |
| AoxI | GGCC | 3 cut(s) | 130, 415, 563 |
| ApaI | GGGCCC | 1 cut(s) | 419 |
| ApeKI | GCWGC | 2 cut(s) | 143, 598 |
| ApoI | RAATTY | 2 cut(s) | 254, 549 |
| AseI | ATTAAT | 1 cut(s) | 437 |
| AspA2I | CCTAGG | 1 cut(s) | 521 |
| AspLEI | GCGC | 4 cut(s) | 20, 323, 471, 473 |
| AspS9I | GGNCC | 4 cut(s) | 74, 136, 415, 416 |
| AsuC2I | CCSGG | 1 cut(s) | 129 |
| AvaII | GGWCC | 2 cut(s) | 74, 136 |
| AvrII | CCTAGG | 1 cut(s) | 521 |
| BaeGI | GKGCMC | 2 cut(s) | 419, 640 |
| BanI | GGYRCC | 1 cut(s) | 635 |
| BanII | GRGCYC | 1 cut(s) | 419 |
| BbvCI | CCTCAGC | 3 cut(s) | 44, 338, 479 |
| BbvI | GCAGC | 2 cut(s) | 130, 610 |
| BciT130I | CCWGG | 1 cut(s) | 134 |
| BcnI | CCSGG | 1 cut(s) | 129 |
| BcoDI | GTCTC | 1 cut(s) | 122 |
| BcuI | ACTAGT | 1 cut(s) | 163 |
| BfaI | CTAG | 3 cut(s) | 164, 491, 522 |
| BfoI | RGCGCY | 1 cut(s) | 324 |
| BisI | GCNGC | 2 cut(s) | 144, 599 |
| BlnI | CCTAGG | 1 cut(s) | 521 |
| BlsI | GCNGC | 2 cut(s) | 145, 600 |
| Bme1390I | CCNGG | 2 cut(s) | 129, 134 |
| Bme18I | GGWCC | 2 cut(s) | 74, 136 |
| BmgT120I | GGNCC | 4 cut(s) | 74, 136, 415, 416 |
| BmiI | GGNNCC | 2 cut(s) | 417, 637 |
| BmrFI | CCNGG | 2 cut(s) | 129, 134 |
| BmrI | ACTGGG | 3 cut(s) | 79, 290, 422 |
| BmuI | ACTGGG | 3 cut(s) | 79, 290, 422 |
| BpmI | CTGGAG | 1 cut(s) | 299 |
| Bpu10I | CCTNAGC | 3 cut(s) | 44, 338, 479 |
| BpuMI | CCSGG | 1 cut(s) | 129 |
| BsaHI | GRCGYC | 1 cut(s) | 570 |
| BsaJI | CCNNGG | 2 cut(s) | 521, 560 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 91, 484 |
| Bse1I | ACTGG | 4 cut(s) | 85, 296, 365, 417 |
| Bse3DI | GCAATG | 1 cut(s) | 349 |
| BseBI | CCWGG | 1 cut(s) | 134 |
| BseDI | CCNNGG | 2 cut(s) | 521, 560 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 91, 484 |
| BseMI | GCAATG | 1 cut(s) | 349 |
| BseMII | CTCAG | 3 cut(s) | 58, 352, 493 |
| BseNI | ACTGG | 4 cut(s) | 85, 296, 365, 417 |
| BsePI | GCGCGC | 1 cut(s) | 469 |
| BseRI | GAGGAG | 2 cut(s) | 414, 417 |
| BseSI | GKGCMC | 2 cut(s) | 419, 640 |
| BseXI | GCAGC | 2 cut(s) | 130, 610 |
| BsgI | GTGCAG | 1 cut(s) | 320 |
| Bsh1236I | CGCG | 2 cut(s) | 18, 471 |
| BshFI | GGCC | 3 cut(s) | 132, 417, 565 |
| BshNI | GGYRCC | 1 cut(s) | 635 |
| BsiSI | CCGG | 1 cut(s) | 129 |
| BslFI | GGGAC | 1 cut(s) | 501 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 91, 484 |
| BsmAI | GTCTC | 1 cut(s) | 122 |
| BsmFI | GGGAC | 1 cut(s) | 501 |
| BsnI | GGCC | 3 cut(s) | 132, 417, 565 |
| Bsp120I | GGGCCC | 1 cut(s) | 415 |
| Bsp1286I | GDGCHC | 2 cut(s) | 419, 640 |
| Bsp143I | GATC | 2 cut(s) | 36, 169 |
| BspACI | CCGC | 5 cut(s) | 16, 77, 386, 419, 632 |
| BspANI | GGCC | 3 cut(s) | 132, 417, 565 |
| BspCNI | CTCAG | 3 cut(s) | 57, 351, 492 |
| BspFNI | CGCG | 2 cut(s) | 18, 471 |
| BspLI | GGNNCC | 2 cut(s) | 417, 637 |
| BspPI | GGATC | 1 cut(s) | 177 |
| BspT107I | GGYRCC | 1 cut(s) | 635 |
| BsrDI | GCAATG | 1 cut(s) | 349 |
| BsrI | ACTGG | 4 cut(s) | 85, 296, 365, 417 |
| BssECI | CCNNGG | 2 cut(s) | 521, 560 |
| BssHII | GCGCGC | 1 cut(s) | 469 |
| BssMI | GATC | 2 cut(s) | 36, 169 |
| BssNI | GRCGYC | 1 cut(s) | 570 |
| BssT1I | CCWWGG | 2 cut(s) | 521, 560 |
| Bst2UI | CCWGG | 1 cut(s) | 134 |
| Bst4CI | ACNGT | 1 cut(s) | 502 |
| Bst6I | CTCTTC | 1 cut(s) | 29 |
| BstACI | GRCGYC | 1 cut(s) | 570 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 595 |
| BstC8I | GCNNGC | 4 cut(s) | 159, 419, 471, 516 |
| BstDEI | CTNAG | 4 cut(s) | 44, 329, 338, 479 |
| BstFNI | CGCG | 2 cut(s) | 18, 471 |
| BstH2I | RGCGCY | 1 cut(s) | 324 |
| BstHHI | GCGC | 4 cut(s) | 20, 323, 471, 473 |
| BstKTI | GATC | 2 cut(s) | 39, 172 |
| BstMAI | GTCTC | 1 cut(s) | 122 |
| BstMBI | GATC | 2 cut(s) | 36, 169 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 595 |
| BstNI | CCWGG | 1 cut(s) | 134 |
| BstSCI | CCNGG | 2 cut(s) | 127, 132 |
| BstSLI | GKGCMC | 2 cut(s) | 419, 640 |
| BstUI | CGCG | 2 cut(s) | 18, 471 |
| BstV1I | GCAGC | 2 cut(s) | 130, 610 |
| BstX2I | RGATCY | 1 cut(s) | 169 |
| BstYI | RGATCY | 1 cut(s) | 169 |
| BsuRI | GGCC | 3 cut(s) | 132, 417, 565 |
| BtsIMutI | CAGTG | 3 cut(s) | 96, 303, 507 |
| Cac8I | GCNNGC | 4 cut(s) | 159, 419, 471, 516 |
| CfoI | GCGC | 4 cut(s) | 20, 323, 471, 473 |
| Cfr13I | GGNCC | 4 cut(s) | 74, 136, 415, 416 |
| CseI | GACGC | 1 cut(s) | 578 |
| Csp6I | GTAC | 1 cut(s) | 383 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 483, 518 |
| CviAII | CATG | 1 cut(s) | 539 |
| CviJI | RGCY | 8 cut(s) | 132, 157, 224, 328, 403, 417, 518, 565 |
| CviKI_1 | RGCY | 8 cut(s) | 132, 157, 224, 328, 403, 417, 518, 565 |
| CviQI | GTAC | 1 cut(s) | 383 |
| DdeI | CTNAG | 4 cut(s) | 44, 329, 338, 479 |
| DpnI | GATC | 2 cut(s) | 38, 171 |
| DpnII | GATC | 2 cut(s) | 36, 169 |
| EaeI | YGGCCR | 1 cut(s) | 130 |
| Eam1104I | CTCTTC | 1 cut(s) | 29 |
| EarI | CTCTTC | 1 cut(s) | 29 |
| Eco130I | CCWWGG | 2 cut(s) | 521, 560 |
| Eco147I | AGGCCT | 1 cut(s) | 565 |
| Eco24I | GRGCYC | 1 cut(s) | 419 |
| Eco47I | GGWCC | 2 cut(s) | 74, 136 |
| EcoO109I | RGGNCCY | 1 cut(s) | 136 |
| EcoRII | CCWGG | 1 cut(s) | 132 |
| EcoT14I | CCWWGG | 2 cut(s) | 521, 560 |
| EcoT38I | GRGCYC | 1 cut(s) | 419 |
| ErhI | CCWWGG | 2 cut(s) | 521, 560 |
| FaeI | CATG | 1 cut(s) | 542 |
| FaiI | YATR | 5 cut(s) | 71, 153, 318, 371, 540 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 51, 83 |
| FaqI | GGGAC | 1 cut(s) | 501 |
| FatI | CATG | 1 cut(s) | 538 |
| FauI | CCCGC | 2 cut(s) | 426, 625 |
| Fnu4HI | GCNGC | 2 cut(s) | 144, 599 |
| FriOI | GRGCYC | 1 cut(s) | 419 |
| Fsp4HI | GCNGC | 2 cut(s) | 144, 599 |
| FspBI | CTAG | 3 cut(s) | 164, 491, 522 |
| GlaI | GCGC | 4 cut(s) | 19, 322, 470, 472 |
| GluI | GCNGC | 2 cut(s) | 144, 599 |
| GsuI | CTGGAG | 1 cut(s) | 299 |
| HaeII | RGCGCY | 1 cut(s) | 324 |
| HaeIII | GGCC | 3 cut(s) | 132, 417, 565 |
| HapII | CCGG | 1 cut(s) | 129 |
| HgaI | GACGC | 1 cut(s) | 578 |
| HhaI | GCGC | 4 cut(s) | 20, 323, 471, 473 |
| Hin1I | GRCGYC | 1 cut(s) | 570 |
| Hin1II | CATG | 1 cut(s) | 542 |
| Hin6I | GCGC | 4 cut(s) | 18, 321, 469, 471 |
| HinP1I | GCGC | 4 cut(s) | 18, 321, 469, 471 |
| HincII | GTYRAC | 1 cut(s) | 7 |
| HindII | GTYRAC | 1 cut(s) | 7 |
| HindIII | AAGCTT | 1 cut(s) | 326 |
| HpaI | GTTAAC | 1 cut(s) | 7 |
| HpaII | CCGG | 1 cut(s) | 129 |
| Hpy166II | GTNNAC | 1 cut(s) | 7 |
| Hpy188I | TCNGA | 2 cut(s) | 36, 623 |
| Hpy8I | GTNNAC | 1 cut(s) | 7 |
| HpyAV | CCTTC | 2 cut(s) | 208, 342 |
| HpyCH4III | ACNGT | 1 cut(s) | 502 |
| HpyCH4IV | ACGT | 1 cut(s) | 381 |
| HpyCH4V | TGCA | 4 cut(s) | 301, 463, 589, 598 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 595 |
| HpyF3I | CTNAG | 4 cut(s) | 44, 329, 338, 479 |
| HpySE526I | ACGT | 1 cut(s) | 381 |
| Hsp92I | GRCGYC | 1 cut(s) | 570 |
| Hsp92II | CATG | 1 cut(s) | 542 |
| HspAI | GCGC | 4 cut(s) | 18, 321, 469, 471 |
| KspAI | GTTAAC | 1 cut(s) | 7 |
| Kzo9I | GATC | 2 cut(s) | 36, 169 |
| LmnI | GCTCC | 1 cut(s) | 196 |
| Lsp1109I | GCAGC | 2 cut(s) | 130, 610 |
| MaeI | CTAG | 3 cut(s) | 164, 491, 522 |
| MaeII | ACGT | 1 cut(s) | 381 |
| MaeIII | GTNAC | 1 cut(s) | 541 |
| MalI | GATC | 2 cut(s) | 38, 171 |
| MboI | GATC | 2 cut(s) | 36, 169 |
| MboII | GAAGA | 2 cut(s) | 16, 620 |
| MflI | RGATCY | 1 cut(s) | 169 |
| MhlI | GDGCHC | 2 cut(s) | 419, 640 |
| MluCI | AATT | 2 cut(s) | 254, 549 |
| MmeI | TCCRAC | 2 cut(s) | 423, 585 |
| MseI | TTAA | 3 cut(s) | 6, 12, 437 |
| MslI | CAYNNNNRTG | 2 cut(s) | 245, 374 |
| MspI | CCGG | 1 cut(s) | 129 |
| MspR9I | CCNGG | 2 cut(s) | 129, 134 |
| MvaI | CCWGG | 1 cut(s) | 134 |
| MvnI | CGCG | 2 cut(s) | 18, 471 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 595 |
| NciI | CCSGG | 1 cut(s) | 129 |
| NdeII | GATC | 2 cut(s) | 36, 169 |
| NlaIII | CATG | 1 cut(s) | 542 |
| NlaIV | GGNNCC | 2 cut(s) | 417, 637 |
| NmuCI | GTSAC | 1 cut(s) | 541 |
| PauI | GCGCGC | 1 cut(s) | 469 |
| PceI | AGGCCT | 1 cut(s) | 565 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 91 |
| PkrI | GCNGC | 2 cut(s) | 145, 600 |
| PpuMI | RGGWCCY | 1 cut(s) | 136 |
| PshBI | ATTAAT | 1 cut(s) | 437 |
| Psp5II | RGGWCCY | 1 cut(s) | 136 |
| Psp6I | CCWGG | 1 cut(s) | 132 |
| PspGI | CCWGG | 1 cut(s) | 132 |
| PspN4I | GGNNCC | 2 cut(s) | 417, 637 |
| PspOMI | GGGCCC | 1 cut(s) | 415 |
| PspPI | GGNCC | 4 cut(s) | 74, 136, 415, 416 |
| PspPPI | RGGWCCY | 1 cut(s) | 136 |
| PsuI | RGATCY | 1 cut(s) | 169 |
| PteI | GCGCGC | 1 cut(s) | 469 |
| RsaI | GTAC | 1 cut(s) | 384 |
| RsaNI | GTAC | 1 cut(s) | 383 |
| RseI | CAYNNNNRTG | 2 cut(s) | 245, 374 |
| SaqAI | TTAA | 3 cut(s) | 6, 12, 437 |
| SatI | GCNGC | 2 cut(s) | 144, 599 |
| Sau3AI | GATC | 2 cut(s) | 36, 169 |
| Sau96I | GGNCC | 4 cut(s) | 74, 136, 415, 416 |
| ScrFI | CCNGG | 2 cut(s) | 129, 134 |
| SduI | GDGCHC | 2 cut(s) | 419, 640 |
| SetI | ASST | 9 cut(s) | 45, 76, 141, 159, 330, 384, 405, 480, 534 |
| SinI | GGWCC | 2 cut(s) | 74, 136 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 245, 374 |
| SpeI | ACTAGT | 1 cut(s) | 163 |
| Sse9I | AATT | 2 cut(s) | 254, 549 |
| SseBI | AGGCCT | 1 cut(s) | 565 |
| SsiI | CCGC | 5 cut(s) | 16, 77, 386, 419, 632 |
| SspMI | CTAG | 3 cut(s) | 164, 491, 522 |
| StuI | AGGCCT | 1 cut(s) | 565 |
| StyD4I | CCNGG | 2 cut(s) | 127, 132 |
| StyI | CCWWGG | 2 cut(s) | 521, 560 |
| TaaI | ACNGT | 1 cut(s) | 502 |
| TaiI | ACGT | 1 cut(s) | 384 |
| TaqI | TCGA | 1 cut(s) | 107 |
| TasI | AATT | 2 cut(s) | 254, 549 |
| Tru1I | TTAA | 3 cut(s) | 6, 12, 437 |
| Tru9I | TTAA | 3 cut(s) | 6, 12, 437 |
| TscAI | CASTG | 3 cut(s) | 96, 303, 507 |
| TseFI | GTSAC | 1 cut(s) | 541 |
| TseI | GCWGC | 2 cut(s) | 143, 598 |
| Tsp45I | GTSAC | 1 cut(s) | 541 |
| TspDTI | ATGAA | 1 cut(s) | 392 |
| TspRI | CASTG | 3 cut(s) | 96, 303, 507 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 91 |
| VpaK11BI | GGWCC | 2 cut(s) | 74, 136 |
| VspI | ATTAAT | 1 cut(s) | 437 |
| XapI | RAATTY | 2 cut(s) | 254, 549 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 154 |
| XmaJI | CCTAGG | 1 cut(s) | 521 |
| XspI | CTAG | 3 cut(s) | 164, 491, 522 |