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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 5 cut(s) | 38, 48, 185, 231, 254 |
| AcoI | YGGCCR | 1 cut(s) | 324 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| AgsI | TTSAA | 1 cut(s) | 308 |
| AjnI | CCWGG | 1 cut(s) | 159 |
| AluBI | AGCT | 2 cut(s) | 138, 280 |
| AluI | AGCT | 2 cut(s) | 138, 280 |
| Alw21I | GWGCWC | 2 cut(s) | 112, 282 |
| Alw26I | GTCTC | 1 cut(s) | 182 |
| Alw44I | GTGCAC | 1 cut(s) | 108 |
| AoxI | GGCC | 2 cut(s) | 217, 324 |
| ApaLI | GTGCAC | 1 cut(s) | 108 |
| ApeKI | GCWGC | 1 cut(s) | 201 |
| AspLEI | GCGC | 1 cut(s) | 294 |
| AspS9I | GGNCC | 3 cut(s) | 40, 163, 256 |
| AsuNHI | GCTAGC | 1 cut(s) | 77 |
| AvaII | GGWCC | 3 cut(s) | 40, 163, 256 |
| AxyI | CCTNAGG | 1 cut(s) | 147 |
| BaeGI | GKGCMC | 1 cut(s) | 112 |
| BalI | TGGCCA | 1 cut(s) | 326 |
| BanII | GRGCYC | 1 cut(s) | 282 |
| Bbv12I | GWGCWC | 2 cut(s) | 112, 282 |
| BbvI | GCAGC | 1 cut(s) | 213 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 1 cut(s) | 161 |
| BcoDI | GTCTC | 1 cut(s) | 182 |
| BfaI | CTAG | 3 cut(s) | 78, 135, 197 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 216 |
| BisI | GCNGC | 2 cut(s) | 202, 231 |
| BlsI | GCNGC | 2 cut(s) | 203, 232 |
| Bme1390I | CCNGG | 1 cut(s) | 161 |
| Bme18I | GGWCC | 3 cut(s) | 40, 163, 256 |
| BmgT120I | GGNCC | 3 cut(s) | 40, 163, 256 |
| BmiI | GGNNCC | 2 cut(s) | 144, 165 |
| BmrFI | CCNGG | 1 cut(s) | 161 |
| BmtI | GCTAGC | 1 cut(s) | 81 |
| BsaI | GGTCTC | 1 cut(s) | 182 |
| BsaJI | CCNNGG | 2 cut(s) | 72, 159 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| Bse1I | ACTGG | 1 cut(s) | 125 |
| Bse21I | CCTNAGG | 1 cut(s) | 147 |
| BseBI | CCWGG | 1 cut(s) | 161 |
| BseDI | CCNNGG | 2 cut(s) | 72, 159 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BseMII | CTCAG | 1 cut(s) | 161 |
| BseNI | ACTGG | 1 cut(s) | 125 |
| BseSI | GKGCMC | 1 cut(s) | 112 |
| BseXI | GCAGC | 1 cut(s) | 213 |
| BshFI | GGCC | 2 cut(s) | 219, 326 |
| BsiHKAI | GWGCWC | 2 cut(s) | 112, 282 |
| BslFI | GGGAC | 1 cut(s) | 149 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BsmAI | GTCTC | 1 cut(s) | 182 |
| BsmFI | GGGAC | 1 cut(s) | 149 |
| BsnI | GGCC | 2 cut(s) | 219, 326 |
| Bso31I | GGTCTC | 1 cut(s) | 182 |
| Bsp1286I | GDGCHC | 2 cut(s) | 112, 282 |
| BspACI | CCGC | 5 cut(s) | 38, 48, 185, 231, 254 |
| BspANI | GGCC | 2 cut(s) | 219, 326 |
| BspCNI | CTCAG | 1 cut(s) | 160 |
| BspLI | GGNNCC | 2 cut(s) | 144, 165 |
| BspOI | GCTAGC | 1 cut(s) | 81 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspTNI | GGTCTC | 1 cut(s) | 182 |
| BsrI | ACTGG | 1 cut(s) | 125 |
| BssECI | CCNNGG | 2 cut(s) | 72, 159 |
| Bst2UI | CCWGG | 1 cut(s) | 161 |
| Bst4CI | ACNGT | 3 cut(s) | 11, 94, 107 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 2 cut(s) | 36, 79 |
| BstDEI | CTNAG | 1 cut(s) | 147 |
| BstDSI | CCRYGG | 1 cut(s) | 72 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstHHI | GCGC | 1 cut(s) | 294 |
| BstMAI | GTCTC | 1 cut(s) | 182 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 207, 216 |
| BstNI | CCWGG | 1 cut(s) | 161 |
| BstSCI | CCNGG | 1 cut(s) | 159 |
| BstSLI | GKGCMC | 1 cut(s) | 112 |
| BstV1I | GCAGC | 1 cut(s) | 213 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 73 |
| Bsu36I | CCTNAGG | 1 cut(s) | 147 |
| BsuRI | GGCC | 2 cut(s) | 219, 326 |
| BtgI | CCRYGG | 1 cut(s) | 72 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BtsIMutI | CAGTG | 1 cut(s) | 167 |
| Cac8I | GCNNGC | 2 cut(s) | 36, 79 |
| CfoI | GCGC | 1 cut(s) | 294 |
| Cfr13I | GGNCC | 3 cut(s) | 40, 163, 256 |
| CviJI | RGCY | 6 cut(s) | 77, 138, 219, 280, 311, 326 |
| CviKI_1 | RGCY | 6 cut(s) | 77, 138, 219, 280, 311, 326 |
| DdeI | CTNAG | 1 cut(s) | 147 |
| EaeI | YGGCCR | 1 cut(s) | 324 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 3 cut(s) | 37, 53, 269 |
| Ecl136II | GAGCTC | 1 cut(s) | 280 |
| Eco24I | GRGCYC | 1 cut(s) | 282 |
| Eco31I | GGTCTC | 1 cut(s) | 182 |
| Eco47I | GGWCC | 3 cut(s) | 40, 163, 256 |
| Eco53kI | GAGCTC | 1 cut(s) | 280 |
| Eco81I | CCTNAGG | 1 cut(s) | 147 |
| EcoICRI | GAGCTC | 1 cut(s) | 280 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoO109I | RGGNCCY | 1 cut(s) | 163 |
| EcoRII | CCWGG | 1 cut(s) | 159 |
| EcoT38I | GRGCYC | 1 cut(s) | 282 |
| FaiI | YATR | 2 cut(s) | 236, 240 |
| FaqI | GGGAC | 1 cut(s) | 149 |
| Fnu4HI | GCNGC | 2 cut(s) | 202, 231 |
| FokI | GGATG | 1 cut(s) | 45 |
| FriOI | GRGCYC | 1 cut(s) | 282 |
| Fsp4HI | GCNGC | 2 cut(s) | 202, 231 |
| FspBI | CTAG | 3 cut(s) | 78, 135, 197 |
| GlaI | GCGC | 1 cut(s) | 293 |
| GluI | GCNGC | 2 cut(s) | 202, 231 |
| HaeIII | GGCC | 2 cut(s) | 219, 326 |
| HhaI | GCGC | 1 cut(s) | 294 |
| Hin6I | GCGC | 1 cut(s) | 292 |
| HinP1I | GCGC | 1 cut(s) | 292 |
| HincII | GTYRAC | 3 cut(s) | 85, 103, 130 |
| HindII | GTYRAC | 3 cut(s) | 85, 103, 130 |
| HinfI | GANTC | 2 cut(s) | 172, 342 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| Hpy166II | GTNNAC | 4 cut(s) | 85, 103, 110, 130 |
| Hpy188III | TCNNGA | 1 cut(s) | 339 |
| Hpy8I | GTNNAC | 4 cut(s) | 85, 103, 110, 130 |
| HpyCH4III | ACNGT | 3 cut(s) | 11, 94, 107 |
| HpyCH4V | TGCA | 1 cut(s) | 110 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 207, 216 |
| HpyF3I | CTNAG | 1 cut(s) | 147 |
| HspAI | GCGC | 1 cut(s) | 292 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 277 |
| LpnPI | CCDG | 5 cut(s) | 20, 134, 138, 146, 173 |
| Lsp1109I | GCAGC | 1 cut(s) | 213 |
| MaeI | CTAG | 3 cut(s) | 78, 135, 197 |
| MaeIII | GTNAC | 1 cut(s) | 151 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MhlI | GDGCHC | 2 cut(s) | 112, 282 |
| MlsI | TGGCCA | 1 cut(s) | 326 |
| MluNI | TGGCCA | 1 cut(s) | 326 |
| MnlI | CCTC | 3 cut(s) | 156, 236, 269 |
| Mox20I | TGGCCA | 1 cut(s) | 326 |
| MscI | TGGCCA | 1 cut(s) | 326 |
| MseI | TTAA | 3 cut(s) | 84, 96, 117 |
| MslI | CAYNNNNRTG | 1 cut(s) | 332 |
| Msp20I | TGGCCA | 1 cut(s) | 326 |
| MspA1I | CMGCKG | 1 cut(s) | 185 |
| MspR9I | CCNGG | 1 cut(s) | 161 |
| MvaI | CCWGG | 1 cut(s) | 161 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 207, 216 |
| NheI | GCTAGC | 1 cut(s) | 77 |
| NlaIV | GGNNCC | 2 cut(s) | 144, 165 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PfeI | GAWTC | 2 cut(s) | 172, 342 |
| PkrI | GCNGC | 2 cut(s) | 203, 232 |
| PpuMI | RGGWCCY | 1 cut(s) | 163 |
| Psp124BI | GAGCTC | 1 cut(s) | 282 |
| Psp5II | RGGWCCY | 1 cut(s) | 163 |
| Psp6I | CCWGG | 1 cut(s) | 159 |
| PspGI | CCWGG | 1 cut(s) | 159 |
| PspN4I | GGNNCC | 2 cut(s) | 144, 165 |
| PspPI | GGNCC | 3 cut(s) | 40, 163, 256 |
| PspPPI | RGGWCCY | 1 cut(s) | 163 |
| RseI | CAYNNNNRTG | 1 cut(s) | 332 |
| SacI | GAGCTC | 1 cut(s) | 282 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 117 |
| SatI | GCNGC | 2 cut(s) | 202, 231 |
| Sau96I | GGNCC | 3 cut(s) | 40, 163, 256 |
| ScrFI | CCNGG | 1 cut(s) | 161 |
| SduI | GDGCHC | 2 cut(s) | 112, 282 |
| SetI | ASST | 8 cut(s) | 140, 148, 153, 165, 228, 261, 282, 306 |
| SinI | GGWCC | 3 cut(s) | 40, 163, 256 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 332 |
| SsiI | CCGC | 5 cut(s) | 38, 48, 185, 231, 254 |
| SspMI | CTAG | 3 cut(s) | 78, 135, 197 |
| SstI | GAGCTC | 1 cut(s) | 282 |
| StyD4I | CCNGG | 1 cut(s) | 159 |
| TaaI | ACNGT | 3 cut(s) | 11, 94, 107 |
| TaqI | TCGA | 2 cut(s) | 282, 345 |
| TauI | GCSGC | 1 cut(s) | 233 |
| TfiI | GAWTC | 2 cut(s) | 172, 342 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 117 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 117 |
| TscAI | CASTG | 1 cut(s) | 174 |
| TseI | GCWGC | 1 cut(s) | 201 |
| TspRI | CASTG | 1 cut(s) | 174 |
| VneI | GTGCAC | 1 cut(s) | 108 |
| VpaK11BI | GGWCC | 3 cut(s) | 40, 163, 256 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 70 |
| XspI | CTAG | 3 cut(s) | 78, 135, 197 |