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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 514 |
| AciI | CCGC | 6 cut(s) | 9, 15, 219, 293, 482, 565 |
| AclWI | GGATC | 1 cut(s) | 219 |
| AcsI | RAATTY | 1 cut(s) | 329 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 416, 571 |
| AgsI | TTSAA | 2 cut(s) | 29, 562 |
| AluBI | AGCT | 4 cut(s) | 299, 340, 473, 541 |
| AluI | AGCT | 4 cut(s) | 299, 340, 473, 541 |
| Alw21I | GWGCWC | 2 cut(s) | 153, 430 |
| AlwI | GGATC | 1 cut(s) | 219 |
| Ama87I | CYCGRG | 1 cut(s) | 143 |
| AoxI | GGCC | 1 cut(s) | 205 |
| ApeKI | GCWGC | 1 cut(s) | 541 |
| ApoI | RAATTY | 1 cut(s) | 329 |
| AspLEI | GCGC | 1 cut(s) | 398 |
| AspS9I | GGNCC | 1 cut(s) | 491 |
| AsuC2I | CCSGG | 1 cut(s) | 209 |
| AsuHPI | GGTGA | 1 cut(s) | 94 |
| AsuNHI | GCTAGC | 1 cut(s) | 5 |
| AvaI | CYCGRG | 1 cut(s) | 143 |
| AvaII | GGWCC | 1 cut(s) | 491 |
| Bbv12I | GWGCWC | 2 cut(s) | 153, 430 |
| BbvI | GCAGC | 1 cut(s) | 528 |
| BccI | CCATC | 1 cut(s) | 14 |
| BcnI | CCSGG | 1 cut(s) | 209 |
| BfaI | CTAG | 3 cut(s) | 6, 165, 479 |
| BfuAI | ACCTGC | 1 cut(s) | 514 |
| BisI | GCNGC | 3 cut(s) | 16, 219, 542 |
| BlsI | GCNGC | 3 cut(s) | 17, 220, 543 |
| Bme1390I | CCNGG | 1 cut(s) | 209 |
| Bme18I | GGWCC | 1 cut(s) | 491 |
| BmeT110I | CYCGRG | 1 cut(s) | 143 |
| BmgT120I | GGNCC | 1 cut(s) | 491 |
| BmiI | GGNNCC | 1 cut(s) | 576 |
| BmrFI | CCNGG | 1 cut(s) | 209 |
| BmtI | GCTAGC | 1 cut(s) | 9 |
| BpuMI | CCSGG | 1 cut(s) | 209 |
| BsaJI | CCNNGG | 2 cut(s) | 144, 237 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 416, 571 |
| Bse118I | RCCGGY | 1 cut(s) | 182 |
| BseDI | CCNNGG | 2 cut(s) | 144, 237 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 416, 571 |
| BseMII | CTCAG | 1 cut(s) | 389 |
| BseXI | GCAGC | 1 cut(s) | 528 |
| BseYI | CCCAGC | 2 cut(s) | 57, 469 |
| BshFI | GGCC | 1 cut(s) | 207 |
| BsiHKAI | GWGCWC | 2 cut(s) | 153, 430 |
| BsiHKCI | CYCGRG | 1 cut(s) | 143 |
| BsiSI | CCGG | 2 cut(s) | 183, 208 |
| BslFI | GGGAC | 1 cut(s) | 433 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 416, 571 |
| BsmFI | GGGAC | 1 cut(s) | 433 |
| BsnI | GGCC | 1 cut(s) | 207 |
| BsoBI | CYCGRG | 1 cut(s) | 143 |
| Bsp1286I | GDGCHC | 2 cut(s) | 153, 430 |
| Bsp143I | GATC | 2 cut(s) | 31, 211 |
| BspACI | CCGC | 6 cut(s) | 9, 15, 219, 293, 482, 565 |
| BspANI | GGCC | 1 cut(s) | 207 |
| BspCNI | CTCAG | 1 cut(s) | 388 |
| BspLI | GGNNCC | 1 cut(s) | 576 |
| BspMI | ACCTGC | 1 cut(s) | 514 |
| BspOI | GCTAGC | 1 cut(s) | 9 |
| BspPI | GGATC | 1 cut(s) | 219 |
| BsrFI | RCCGGY | 1 cut(s) | 182 |
| BssAI | RCCGGY | 1 cut(s) | 182 |
| BssECI | CCNNGG | 2 cut(s) | 144, 237 |
| BssMI | GATC | 2 cut(s) | 31, 211 |
| BssT1I | CCWWGG | 1 cut(s) | 237 |
| Bst4CI | ACNGT | 5 cut(s) | 85, 92, 125, 324, 510 |
| BstC8I | GCNNGC | 1 cut(s) | 7 |
| BstDEI | CTNAG | 2 cut(s) | 375, 404 |
| BstHHI | GCGC | 1 cut(s) | 398 |
| BstKTI | GATC | 2 cut(s) | 34, 214 |
| BstMBI | GATC | 2 cut(s) | 31, 211 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 15, 21, 479 |
| BstSCI | CCNGG | 1 cut(s) | 207 |
| BstV1I | GCAGC | 1 cut(s) | 528 |
| BstX2I | RGATCY | 1 cut(s) | 211 |
| BstYI | RGATCY | 1 cut(s) | 211 |
| BsuRI | GGCC | 1 cut(s) | 207 |
| BtgZI | GCGATG | 1 cut(s) | 33 |
| BveI | ACCTGC | 1 cut(s) | 514 |
| Cac8I | GCNNGC | 1 cut(s) | 7 |
| CfoI | GCGC | 1 cut(s) | 398 |
| Cfr10I | RCCGGY | 1 cut(s) | 182 |
| Cfr13I | GGNCC | 1 cut(s) | 491 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 99, 134, 492, 527 |
| CviAII | CATG | 1 cut(s) | 187 |
| CviJI | RGCY | 9 cut(s) | 5, 61, 207, 236, 299, 340, 403, 473, 541 |
| CviKI_1 | RGCY | 9 cut(s) | 5, 61, 207, 236, 299, 340, 403, 473, 541 |
| DdeI | CTNAG | 2 cut(s) | 375, 404 |
| DpnI | GATC | 2 cut(s) | 33, 213 |
| DpnII | GATC | 2 cut(s) | 31, 211 |
| EciI | GGCGGA | 1 cut(s) | 308 |
| Eco130I | CCWWGG | 1 cut(s) | 237 |
| Eco47I | GGWCC | 1 cut(s) | 491 |
| Eco88I | CYCGRG | 1 cut(s) | 143 |
| EcoT14I | CCWWGG | 1 cut(s) | 237 |
| ErhI | CCWWGG | 1 cut(s) | 237 |
| FaeI | CATG | 1 cut(s) | 190 |
| FaiI | YATR | 7 cut(s) | 188, 224, 252, 416, 443, 549, 586 |
| FaqI | GGGAC | 1 cut(s) | 433 |
| FatI | CATG | 1 cut(s) | 186 |
| FauI | CCCGC | 1 cut(s) | 475 |
| Fnu4HI | GCNGC | 3 cut(s) | 16, 219, 542 |
| Fsp4HI | GCNGC | 3 cut(s) | 16, 219, 542 |
| FspBI | CTAG | 3 cut(s) | 6, 165, 479 |
| GlaI | GCGC | 1 cut(s) | 397 |
| GluI | GCNGC | 3 cut(s) | 16, 219, 542 |
| GsaI | CCCAGC | 2 cut(s) | 61, 473 |
| HaeIII | GGCC | 1 cut(s) | 207 |
| HapII | CCGG | 2 cut(s) | 183, 208 |
| HhaI | GCGC | 1 cut(s) | 398 |
| Hin1II | CATG | 1 cut(s) | 190 |
| Hin6I | GCGC | 1 cut(s) | 396 |
| HinP1I | GCGC | 1 cut(s) | 396 |
| HincII | GTYRAC | 3 cut(s) | 44, 52, 88 |
| HindII | GTYRAC | 3 cut(s) | 44, 52, 88 |
| HinfI | GANTC | 1 cut(s) | 533 |
| HpaII | CCGG | 2 cut(s) | 183, 208 |
| HphI | GGTGA | 1 cut(s) | 94 |
| Hpy166II | GTNNAC | 6 cut(s) | 44, 52, 88, 102, 121, 383 |
| Hpy188I | TCNGA | 2 cut(s) | 180, 378 |
| Hpy188III | TCNNGA | 1 cut(s) | 165 |
| Hpy8I | GTNNAC | 6 cut(s) | 44, 52, 88, 102, 121, 383 |
| HpyAV | CCTTC | 2 cut(s) | 276, 340 |
| HpyCH4III | ACNGT | 5 cut(s) | 85, 92, 125, 324, 510 |
| HpyCH4IV | ACGT | 1 cut(s) | 117 |
| HpyCH4V | TGCA | 2 cut(s) | 250, 544 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 15, 21, 479 |
| HpyF3I | CTNAG | 2 cut(s) | 375, 404 |
| HpySE526I | ACGT | 1 cut(s) | 117 |
| Hsp92II | CATG | 1 cut(s) | 190 |
| HspAI | GCGC | 1 cut(s) | 396 |
| Kzo9I | GATC | 2 cut(s) | 31, 211 |
| LmnI | GCTCC | 1 cut(s) | 156 |
| LpnPI | CCDG | 6 cut(s) | 71, 196, 221, 483, 509, 591 |
| Lsp1109I | GCAGC | 1 cut(s) | 528 |
| MaeI | CTAG | 3 cut(s) | 6, 165, 479 |
| MaeII | ACGT | 1 cut(s) | 117 |
| MalI | GATC | 2 cut(s) | 33, 213 |
| MboI | GATC | 2 cut(s) | 31, 211 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MflI | RGATCY | 1 cut(s) | 211 |
| MhlI | GDGCHC | 2 cut(s) | 153, 430 |
| MluCI | AATT | 2 cut(s) | 95, 329 |
| MlyI | GAGTC | 1 cut(s) | 527 |
| MmeI | TCCRAC | 2 cut(s) | 101, 207 |
| MnlI | CCTC | 2 cut(s) | 139, 257 |
| MspA1I | CMGCKG | 2 cut(s) | 473, 541 |
| MspI | CCGG | 2 cut(s) | 183, 208 |
| MspR9I | CCNGG | 1 cut(s) | 209 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 15, 21, 479 |
| NciI | CCSGG | 1 cut(s) | 209 |
| NdeII | GATC | 2 cut(s) | 31, 211 |
| NheI | GCTAGC | 1 cut(s) | 5 |
| NlaIII | CATG | 1 cut(s) | 190 |
| NlaIV | GGNNCC | 1 cut(s) | 576 |
| PkrI | GCNGC | 3 cut(s) | 17, 220, 543 |
| PleI | GAGTC | 1 cut(s) | 527 |
| PpsI | GAGTC | 1 cut(s) | 527 |
| PspFI | CCCAGC | 2 cut(s) | 57, 469 |
| PspN4I | GGNNCC | 1 cut(s) | 576 |
| PspPI | GGNCC | 1 cut(s) | 491 |
| PsuI | RGATCY | 1 cut(s) | 211 |
| PvuII | CAGCTG | 2 cut(s) | 473, 541 |
| SatI | GCNGC | 3 cut(s) | 16, 219, 542 |
| Sau3AI | GATC | 2 cut(s) | 31, 211 |
| Sau96I | GGNCC | 1 cut(s) | 491 |
| SchI | GAGTC | 1 cut(s) | 527 |
| ScrFI | CCNGG | 1 cut(s) | 209 |
| SduI | GDGCHC | 2 cut(s) | 153, 430 |
| SetI | ASST | 8 cut(s) | 120, 150, 301, 342, 442, 475, 528, 543 |
| SinI | GGWCC | 1 cut(s) | 491 |
| Sse9I | AATT | 2 cut(s) | 95, 329 |
| SsiI | CCGC | 6 cut(s) | 9, 15, 219, 293, 482, 565 |
| SspMI | CTAG | 3 cut(s) | 6, 165, 479 |
| StyD4I | CCNGG | 1 cut(s) | 207 |
| StyI | CCWWGG | 1 cut(s) | 237 |
| TaaI | ACNGT | 5 cut(s) | 85, 92, 125, 324, 510 |
| TaiI | ACGT | 1 cut(s) | 120 |
| TasI | AATT | 2 cut(s) | 95, 329 |
| TauI | GCSGC | 2 cut(s) | 18, 221 |
| TseI | GCWGC | 1 cut(s) | 541 |
| VpaK11BI | GGWCC | 1 cut(s) | 491 |
| XapI | RAATTY | 1 cut(s) | 329 |
| XbaI | TCTAGA | 1 cut(s) | 164 |
| XspI | CTAG | 3 cut(s) | 6, 165, 479 |