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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 450 |
| Acc36I | ACCTGC | 1 cut(s) | 450 |
| AccII | CGCG | 1 cut(s) | 169 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AclWI | GGATC | 1 cut(s) | 237 |
| AcoI | YGGCCR | 2 cut(s) | 126, 297 |
| AfaI | GTAC | 1 cut(s) | 172 |
| AfeI | AGCGCT | 2 cut(s) | 139, 382 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 217, 403 |
| AflII | CTTAAG | 1 cut(s) | 266 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AluBI | AGCT | 2 cut(s) | 284, 463 |
| AluI | AGCT | 2 cut(s) | 284, 463 |
| AlwI | GGATC | 1 cut(s) | 237 |
| Ama87I | CYCGRG | 1 cut(s) | 196 |
| Aor51HI | AGCGCT | 2 cut(s) | 139, 382 |
| AoxI | GGCC | 2 cut(s) | 126, 297 |
| ApeKI | GCWGC | 2 cut(s) | 182, 201 |
| AspLEI | GCGC | 4 cut(s) | 140, 337, 383, 545 |
| AspS9I | GGNCC | 2 cut(s) | 40, 476 |
| AsuHPI | GGTGA | 1 cut(s) | 185 |
| AvaI | CYCGRG | 1 cut(s) | 196 |
| AvaII | GGWCC | 2 cut(s) | 40, 476 |
| BalI | TGGCCA | 1 cut(s) | 299 |
| BbvI | GCAGC | 2 cut(s) | 194, 213 |
| BfaI | CTAG | 4 cut(s) | 135, 224, 384, 610 |
| BfoI | RGCGCY | 2 cut(s) | 141, 384 |
| BfrI | CTTAAG | 1 cut(s) | 266 |
| BfuAI | ACCTGC | 1 cut(s) | 450 |
| BisI | GCNGC | 6 cut(s) | 48, 93, 183, 202, 394, 446 |
| BlsI | GCNGC | 6 cut(s) | 49, 94, 184, 203, 395, 447 |
| Bme18I | GGWCC | 2 cut(s) | 40, 476 |
| BmeT110I | CYCGRG | 1 cut(s) | 196 |
| BmgT120I | GGNCC | 2 cut(s) | 40, 476 |
| BmiI | GGNNCC | 3 cut(s) | 165, 477, 605 |
| BmrI | ACTGGG | 1 cut(s) | 482 |
| BmsI | GCATC | 1 cut(s) | 426 |
| BmuI | ACTGGG | 1 cut(s) | 482 |
| Bpu10I | CCTNAGC | 1 cut(s) | 459 |
| BsaJI | CCNNGG | 3 cut(s) | 72, 538, 561 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 217, 403 |
| Bse1I | ACTGG | 2 cut(s) | 425, 477 |
| BseDI | CCNNGG | 3 cut(s) | 72, 538, 561 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 217, 403 |
| BseMII | CTCAG | 3 cut(s) | 299, 412, 450 |
| BseNI | ACTGG | 2 cut(s) | 425, 477 |
| BseRI | GAGGAG | 2 cut(s) | 147, 474 |
| BseXI | GCAGC | 2 cut(s) | 194, 213 |
| BsgI | GTGCAG | 1 cut(s) | 91 |
| Bsh1236I | CGCG | 1 cut(s) | 169 |
| BshFI | GGCC | 2 cut(s) | 128, 299 |
| BsiHKCI | CYCGRG | 1 cut(s) | 196 |
| BsiSI | CCGG | 1 cut(s) | 125 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 217, 403 |
| BsnI | GGCC | 2 cut(s) | 128, 299 |
| BsoBI | CYCGRG | 1 cut(s) | 196 |
| Bsp143I | GATC | 1 cut(s) | 229 |
| Bsp19I | CCATGG | 1 cut(s) | 72 |
| BspANI | GGCC | 2 cut(s) | 128, 299 |
| BspCNI | CTCAG | 3 cut(s) | 298, 411, 451 |
| BspFNI | CGCG | 1 cut(s) | 169 |
| BspLI | GGNNCC | 3 cut(s) | 165, 477, 605 |
| BspMI | ACCTGC | 1 cut(s) | 450 |
| BspPI | GGATC | 1 cut(s) | 237 |
| BspTI | CTTAAG | 1 cut(s) | 266 |
| BsrI | ACTGG | 2 cut(s) | 425, 477 |
| BssECI | CCNNGG | 3 cut(s) | 72, 538, 561 |
| BssMI | GATC | 1 cut(s) | 229 |
| BssT1I | CCWWGG | 2 cut(s) | 72, 561 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 350 |
| Bst6I | CTCTTC | 2 cut(s) | 119, 507 |
| BstAFI | CTTAAG | 1 cut(s) | 266 |
| BstC8I | GCNNGC | 1 cut(s) | 368 |
| BstDEI | CTNAG | 3 cut(s) | 285, 398, 459 |
| BstDSI | CCRYGG | 2 cut(s) | 72, 538 |
| BstEII | GGTNACC | 1 cut(s) | 350 |
| BstFNI | CGCG | 1 cut(s) | 169 |
| BstH2I | RGCGCY | 2 cut(s) | 141, 384 |
| BstHHI | GCGC | 4 cut(s) | 140, 337, 383, 545 |
| BstKTI | GATC | 1 cut(s) | 232 |
| BstMBI | GATC | 1 cut(s) | 229 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 44, 207, 445, 551 |
| BstPI | GGTNACC | 1 cut(s) | 350 |
| BstUI | CGCG | 1 cut(s) | 169 |
| BstV1I | GCAGC | 2 cut(s) | 194, 213 |
| BstX2I | RGATCY | 1 cut(s) | 229 |
| BstYI | RGATCY | 1 cut(s) | 229 |
| BsuRI | GGCC | 2 cut(s) | 128, 299 |
| BtgI | CCRYGG | 2 cut(s) | 72, 538 |
| BtsIMutI | CAGTG | 1 cut(s) | 406 |
| BveI | ACCTGC | 1 cut(s) | 450 |
| Cac8I | GCNNGC | 1 cut(s) | 368 |
| CfoI | GCGC | 4 cut(s) | 140, 337, 383, 545 |
| Cfr13I | GGNCC | 2 cut(s) | 40, 476 |
| Csp6I | GTAC | 1 cut(s) | 171 |
| CviAII | CATG | 2 cut(s) | 73, 587 |
| CviJI | RGCY | 9 cut(s) | 47, 77, 95, 128, 284, 299, 393, 463, 578 |
| CviKI_1 | RGCY | 9 cut(s) | 47, 77, 95, 128, 284, 299, 393, 463, 578 |
| CviQI | GTAC | 1 cut(s) | 171 |
| DdeI | CTNAG | 3 cut(s) | 285, 398, 459 |
| DpnI | GATC | 1 cut(s) | 231 |
| DpnII | GATC | 1 cut(s) | 229 |
| EaeI | YGGCCR | 2 cut(s) | 126, 297 |
| Eam1104I | CTCTTC | 2 cut(s) | 119, 507 |
| EarI | CTCTTC | 2 cut(s) | 119, 507 |
| EciI | GGCGGA | 2 cut(s) | 293, 477 |
| Eco130I | CCWWGG | 2 cut(s) | 72, 561 |
| Eco47I | GGWCC | 2 cut(s) | 40, 476 |
| Eco47III | AGCGCT | 2 cut(s) | 139, 382 |
| Eco88I | CYCGRG | 1 cut(s) | 196 |
| Eco91I | GGTNACC | 1 cut(s) | 350 |
| EcoO65I | GGTNACC | 1 cut(s) | 350 |
| EcoT14I | CCWWGG | 2 cut(s) | 72, 561 |
| ErhI | CCWWGG | 2 cut(s) | 72, 561 |
| FaeI | CATG | 2 cut(s) | 76, 590 |
| FaiI | YATR | 3 cut(s) | 74, 431, 588 |
| FatI | CATG | 2 cut(s) | 72, 586 |
| Fnu4HI | GCNGC | 6 cut(s) | 48, 93, 183, 202, 394, 446 |
| Fsp4HI | GCNGC | 6 cut(s) | 48, 93, 183, 202, 394, 446 |
| FspBI | CTAG | 4 cut(s) | 135, 224, 384, 610 |
| GlaI | GCGC | 4 cut(s) | 139, 336, 382, 544 |
| GluI | GCNGC | 6 cut(s) | 48, 93, 183, 202, 394, 446 |
| HaeII | RGCGCY | 2 cut(s) | 141, 384 |
| HaeIII | GGCC | 2 cut(s) | 128, 299 |
| HapII | CCGG | 1 cut(s) | 125 |
| HhaI | GCGC | 4 cut(s) | 140, 337, 383, 545 |
| Hin1II | CATG | 2 cut(s) | 76, 590 |
| Hin6I | GCGC | 4 cut(s) | 138, 335, 381, 543 |
| HinP1I | GCGC | 4 cut(s) | 138, 335, 381, 543 |
| HincII | GTYRAC | 3 cut(s) | 85, 103, 594 |
| HindII | GTYRAC | 3 cut(s) | 85, 103, 594 |
| HinfI | GANTC | 1 cut(s) | 518 |
| HpaI | GTTAAC | 3 cut(s) | 85, 103, 594 |
| HpaII | CCGG | 1 cut(s) | 125 |
| HphI | GGTGA | 1 cut(s) | 185 |
| Hpy166II | GTNNAC | 5 cut(s) | 85, 103, 346, 479, 594 |
| Hpy188I | TCNGA | 1 cut(s) | 288 |
| Hpy8I | GTNNAC | 5 cut(s) | 85, 103, 346, 479, 594 |
| HpyAV | CCTTC | 2 cut(s) | 268, 544 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 350 |
| HpyCH4IV | ACGT | 1 cut(s) | 81 |
| HpyCH4V | TGCA | 2 cut(s) | 108, 439 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 44, 207, 445, 551 |
| HpyF3I | CTNAG | 3 cut(s) | 285, 398, 459 |
| HpySE526I | ACGT | 1 cut(s) | 81 |
| Hsp92II | CATG | 2 cut(s) | 76, 590 |
| HspAI | GCGC | 4 cut(s) | 138, 335, 381, 543 |
| KspAI | GTTAAC | 3 cut(s) | 85, 103, 594 |
| Kzo9I | GATC | 1 cut(s) | 229 |
| LmnI | GCTCC | 2 cut(s) | 127, 250 |
| Lsp1109I | GCAGC | 2 cut(s) | 194, 213 |
| LweI | GCATC | 1 cut(s) | 426 |
| MaeI | CTAG | 4 cut(s) | 135, 224, 384, 610 |
| MaeII | ACGT | 1 cut(s) | 81 |
| MaeIII | GTNAC | 3 cut(s) | 151, 350, 372 |
| MalI | GATC | 1 cut(s) | 231 |
| MboI | GATC | 1 cut(s) | 229 |
| MboII | GAAGA | 2 cut(s) | 106, 524 |
| MflI | RGATCY | 1 cut(s) | 229 |
| MlsI | TGGCCA | 1 cut(s) | 299 |
| MluNI | TGGCCA | 1 cut(s) | 299 |
| MnlI | CCTC | 5 cut(s) | 168, 407, 492, 495, 508 |
| Mox20I | TGGCCA | 1 cut(s) | 299 |
| MscI | TGGCCA | 1 cut(s) | 299 |
| MseI | TTAA | 5 cut(s) | 84, 102, 267, 497, 593 |
| MslI | CAYNNNNRTG | 2 cut(s) | 305, 434 |
| Msp20I | TGGCCA | 1 cut(s) | 299 |
| MspA1I | CMGCKG | 1 cut(s) | 217 |
| MspCI | CTTAAG | 1 cut(s) | 266 |
| MspI | CCGG | 1 cut(s) | 125 |
| MvnI | CGCG | 1 cut(s) | 169 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 44, 207, 445, 551 |
| NcoI | CCATGG | 1 cut(s) | 72 |
| NdeII | GATC | 1 cut(s) | 229 |
| NlaIII | CATG | 2 cut(s) | 76, 590 |
| NlaIV | GGNNCC | 3 cut(s) | 165, 477, 605 |
| NmuCI | GTSAC | 2 cut(s) | 151, 372 |
| PaqCI | CACCTGC | 1 cut(s) | 450 |
| PfeI | GAWTC | 1 cut(s) | 518 |
| PflFI | GACNNNGTC | 1 cut(s) | 474 |
| PkrI | GCNGC | 6 cut(s) | 49, 94, 184, 203, 395, 447 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| PspEI | GGTNACC | 1 cut(s) | 350 |
| PspN4I | GGNNCC | 3 cut(s) | 165, 477, 605 |
| PspPI | GGNCC | 2 cut(s) | 40, 476 |
| PsuI | RGATCY | 1 cut(s) | 229 |
| PsyI | GACNNNGTC | 1 cut(s) | 474 |
| RsaI | GTAC | 1 cut(s) | 172 |
| RsaNI | GTAC | 1 cut(s) | 171 |
| RseI | CAYNNNNRTG | 2 cut(s) | 305, 434 |
| SaqAI | TTAA | 5 cut(s) | 84, 102, 267, 497, 593 |
| SatI | GCNGC | 6 cut(s) | 48, 93, 183, 202, 394, 446 |
| Sau3AI | GATC | 1 cut(s) | 229 |
| Sau96I | GGNCC | 2 cut(s) | 40, 476 |
| SfaNI | GCATC | 1 cut(s) | 426 |
| SinI | GGWCC | 2 cut(s) | 40, 476 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 305, 434 |
| SmlI | CTYRAG | 1 cut(s) | 266 |
| SmoI | CTYRAG | 1 cut(s) | 266 |
| SspMI | CTAG | 4 cut(s) | 135, 224, 384, 610 |
| StyI | CCWWGG | 2 cut(s) | 72, 561 |
| TaaI | ACNGT | 2 cut(s) | 11, 350 |
| TaiI | ACGT | 1 cut(s) | 84 |
| TauI | GCSGC | 4 cut(s) | 50, 95, 396, 448 |
| TfiI | GAWTC | 1 cut(s) | 518 |
| Tru1I | TTAA | 5 cut(s) | 84, 102, 267, 497, 593 |
| Tru9I | TTAA | 5 cut(s) | 84, 102, 267, 497, 593 |
| TscAI | CASTG | 1 cut(s) | 406 |
| TseFI | GTSAC | 2 cut(s) | 151, 372 |
| TseI | GCWGC | 2 cut(s) | 182, 201 |
| Tsp45I | GTSAC | 2 cut(s) | 151, 372 |
| TspGWI | ACGGA | 1 cut(s) | 527 |
| TspRI | CASTG | 1 cut(s) | 406 |
| Tth111I | GACNNNGTC | 1 cut(s) | 474 |
| Vha464I | CTTAAG | 1 cut(s) | 266 |
| VpaK11BI | GGWCC | 2 cut(s) | 40, 476 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 70 |
| XspI | CTAG | 4 cut(s) | 135, 224, 384, 610 |