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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 431, 439 |
| AccBSI | CCGCTC | 1 cut(s) | 276 |
| AccII | CGCG | 2 cut(s) | 139, 464 |
| AciI | CCGC | 9 cut(s) | 5, 91, 203, 251, 274, 312, 337, 422, 486 |
| AclWI | GGATC | 2 cut(s) | 15, 334 |
| AcuI | CTGAAG | 1 cut(s) | 158 |
| AfaI | GTAC | 1 cut(s) | 343 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 318, 323 |
| AgsI | TTSAA | 1 cut(s) | 450 |
| AjnI | CCWGG | 3 cut(s) | 317, 467, 472 |
| AleI | CACNNNNGTG | 1 cut(s) | 83 |
| AluBI | AGCT | 3 cut(s) | 199, 374, 446 |
| AluI | AGCT | 3 cut(s) | 199, 374, 446 |
| AlwI | GGATC | 2 cut(s) | 15, 334 |
| Ama87I | CYCGRG | 1 cut(s) | 285 |
| AoxI | GGCC | 3 cut(s) | 54, 60, 470 |
| ApeKI | GCWGC | 2 cut(s) | 394, 488 |
| AseI | ATTAAT | 1 cut(s) | 233 |
| AspLEI | GCGC | 1 cut(s) | 139 |
| AspS9I | GGNCC | 4 cut(s) | 61, 178, 212, 476 |
| AsuC2I | CCSGG | 2 cut(s) | 346, 437 |
| AsuNHI | GCTAGC | 1 cut(s) | 199 |
| AvaI | CYCGRG | 1 cut(s) | 285 |
| AvaII | GGWCC | 3 cut(s) | 178, 212, 476 |
| BanI | GGYRCC | 2 cut(s) | 431, 439 |
| BbvI | GCAGC | 2 cut(s) | 406, 475 |
| BceAI | ACGGC | 1 cut(s) | 149 |
| BciT130I | CCWGG | 3 cut(s) | 319, 469, 474 |
| BcnI | CCSGG | 2 cut(s) | 346, 437 |
| BfaI | CTAG | 2 cut(s) | 200, 350 |
| BisI | GCNGC | 2 cut(s) | 395, 489 |
| BlsI | GCNGC | 2 cut(s) | 396, 490 |
| Bme1390I | CCNGG | 5 cut(s) | 319, 346, 437, 469, 474 |
| Bme18I | GGWCC | 3 cut(s) | 178, 212, 476 |
| BmeT110I | CYCGRG | 1 cut(s) | 285 |
| BmgT120I | GGNCC | 4 cut(s) | 61, 178, 212, 476 |
| BmiI | GGNNCC | 4 cut(s) | 180, 213, 433, 441 |
| BmrFI | CCNGG | 5 cut(s) | 319, 346, 437, 469, 474 |
| BmsI | GCATC | 3 cut(s) | 178, 355, 381 |
| BmtI | GCTAGC | 1 cut(s) | 203 |
| BpuMI | CCSGG | 2 cut(s) | 346, 437 |
| BsaJI | CCNNGG | 4 cut(s) | 57, 284, 317, 435 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 239, 269 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 318, 323 |
| Bse1I | ACTGG | 1 cut(s) | 387 |
| Bse3DI | GCAATG | 1 cut(s) | 159 |
| BseBI | CCWGG | 3 cut(s) | 319, 469, 474 |
| BseDI | CCNNGG | 4 cut(s) | 57, 284, 317, 435 |
| BseGI | GGATG | 1 cut(s) | 396 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 318, 323 |
| BseMI | GCAATG | 1 cut(s) | 159 |
| BseNI | ACTGG | 1 cut(s) | 387 |
| BseXI | GCAGC | 2 cut(s) | 406, 475 |
| BseYI | CCCAGC | 1 cut(s) | 133 |
| BsgI | GTGCAG | 2 cut(s) | 170, 248 |
| Bsh1236I | CGCG | 2 cut(s) | 139, 464 |
| BshFI | GGCC | 3 cut(s) | 56, 62, 472 |
| BshNI | GGYRCC | 2 cut(s) | 431, 439 |
| BsiHKCI | CYCGRG | 1 cut(s) | 285 |
| BsiSI | CCGG | 2 cut(s) | 346, 437 |
| BslFI | GGGAC | 3 cut(s) | 73, 164, 225 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 318, 323 |
| BsmFI | GGGAC | 3 cut(s) | 73, 164, 225 |
| BsnI | GGCC | 3 cut(s) | 56, 62, 472 |
| BsoBI | CYCGRG | 1 cut(s) | 285 |
| Bsp143I | GATC | 2 cut(s) | 7, 326 |
| BspACI | CCGC | 9 cut(s) | 5, 91, 203, 251, 274, 312, 337, 422, 486 |
| BspANI | GGCC | 3 cut(s) | 56, 62, 472 |
| BspFNI | CGCG | 2 cut(s) | 139, 464 |
| BspLI | GGNNCC | 4 cut(s) | 180, 213, 433, 441 |
| BspOI | GCTAGC | 1 cut(s) | 203 |
| BspPI | GGATC | 2 cut(s) | 15, 334 |
| BspT107I | GGYRCC | 2 cut(s) | 431, 439 |
| BsrBI | CCGCTC | 1 cut(s) | 276 |
| BsrDI | GCAATG | 1 cut(s) | 159 |
| BsrI | ACTGG | 1 cut(s) | 387 |
| BssECI | CCNNGG | 4 cut(s) | 57, 284, 317, 435 |
| BssMI | GATC | 2 cut(s) | 7, 326 |
| Bst2UI | CCWGG | 3 cut(s) | 319, 469, 474 |
| BstC8I | GCNNGC | 2 cut(s) | 146, 201 |
| BstF5I | GGATG | 1 cut(s) | 396 |
| BstFNI | CGCG | 2 cut(s) | 139, 464 |
| BstHHI | GCGC | 1 cut(s) | 139 |
| BstKTI | GATC | 2 cut(s) | 10, 329 |
| BstMBI | GATC | 2 cut(s) | 7, 326 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 134, 145 |
| BstNI | CCWGG | 3 cut(s) | 319, 469, 474 |
| BstNSI | RCATGY | 1 cut(s) | 74 |
| BstSCI | CCNGG | 5 cut(s) | 317, 344, 435, 467, 472 |
| BstUI | CGCG | 2 cut(s) | 139, 464 |
| BstV1I | GCAGC | 2 cut(s) | 406, 475 |
| BstX2I | RGATCY | 1 cut(s) | 7 |
| BstYI | RGATCY | 1 cut(s) | 7 |
| BsuRI | GGCC | 3 cut(s) | 56, 62, 472 |
| BtgZI | GCGATG | 1 cut(s) | 378 |
| BtsCI | GGATG | 1 cut(s) | 396 |
| BtsI | GCAGTG | 3 cut(s) | 123, 182, 260 |
| BtsIMutI | CAGTG | 3 cut(s) | 123, 182, 260 |
| Cac8I | GCNNGC | 2 cut(s) | 146, 201 |
| CfoI | GCGC | 1 cut(s) | 139 |
| Cfr13I | GGNCC | 4 cut(s) | 61, 178, 212, 476 |
| CseI | GACGC | 1 cut(s) | 453 |
| Csp6I | GTAC | 1 cut(s) | 342 |
| CviAII | CATG | 1 cut(s) | 71 |
| CviJI | RGCY | 9 cut(s) | 56, 62, 77, 122, 199, 349, 374, 446, 472 |
| CviKI_1 | RGCY | 9 cut(s) | 56, 62, 77, 122, 199, 349, 374, 446, 472 |
| CviQI | GTAC | 1 cut(s) | 342 |
| DpnI | GATC | 2 cut(s) | 9, 328 |
| DpnII | GATC | 2 cut(s) | 7, 326 |
| EciI | GGCGGA | 3 cut(s) | 20, 266, 437 |
| Eco47I | GGWCC | 3 cut(s) | 178, 212, 476 |
| Eco57I | CTGAAG | 1 cut(s) | 158 |
| Eco88I | CYCGRG | 1 cut(s) | 285 |
| EcoO109I | RGGNCCY | 1 cut(s) | 178 |
| EcoRII | CCWGG | 3 cut(s) | 317, 467, 472 |
| FaeI | CATG | 1 cut(s) | 74 |
| FaiI | YATR | 2 cut(s) | 72, 224 |
| FaqI | GGGAC | 3 cut(s) | 73, 164, 225 |
| FatI | CATG | 1 cut(s) | 70 |
| FauI | CCCGC | 3 cut(s) | 98, 196, 319 |
| Fnu4HI | GCNGC | 2 cut(s) | 395, 489 |
| FokI | GGATG | 1 cut(s) | 403 |
| Fsp4HI | GCNGC | 2 cut(s) | 395, 489 |
| FspBI | CTAG | 2 cut(s) | 200, 350 |
| GlaI | GCGC | 1 cut(s) | 138 |
| GluI | GCNGC | 2 cut(s) | 395, 489 |
| GsaI | CCCAGC | 1 cut(s) | 137 |
| HaeIII | GGCC | 3 cut(s) | 56, 62, 472 |
| HapII | CCGG | 2 cut(s) | 346, 437 |
| HgaI | GACGC | 1 cut(s) | 453 |
| HhaI | GCGC | 1 cut(s) | 139 |
| Hin1II | CATG | 1 cut(s) | 74 |
| Hin6I | GCGC | 1 cut(s) | 137 |
| HinP1I | GCGC | 1 cut(s) | 137 |
| HinfI | GANTC | 2 cut(s) | 254, 425 |
| HpaII | CCGG | 2 cut(s) | 346, 437 |
| Hpy166II | GTNNAC | 1 cut(s) | 35 |
| Hpy188III | TCNNGA | 1 cut(s) | 153 |
| Hpy8I | GTNNAC | 1 cut(s) | 35 |
| Hpy99I | CGWCG | 1 cut(s) | 362 |
| HpyCH4IV | ACGT | 1 cut(s) | 31 |
| HpyCH4V | TGCA | 6 cut(s) | 169, 187, 265, 368, 394, 491 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 134, 145 |
| HpySE526I | ACGT | 1 cut(s) | 31 |
| Hsp92II | CATG | 1 cut(s) | 74 |
| HspAI | GCGC | 1 cut(s) | 137 |
| Kzo9I | GATC | 2 cut(s) | 7, 326 |
| Lsp1109I | GCAGC | 2 cut(s) | 406, 475 |
| LweI | GCATC | 3 cut(s) | 178, 355, 381 |
| MaeI | CTAG | 2 cut(s) | 200, 350 |
| MaeII | ACGT | 1 cut(s) | 31 |
| MaeIII | GTNAC | 1 cut(s) | 150 |
| MalI | GATC | 2 cut(s) | 9, 328 |
| MbiI | CCGCTC | 1 cut(s) | 276 |
| MboI | GATC | 2 cut(s) | 7, 326 |
| MboII | GAAGA | 1 cut(s) | 164 |
| MflI | RGATCY | 1 cut(s) | 7 |
| MluCI | AATT | 3 cut(s) | 230, 414, 492 |
| MlyI | GAGTC | 1 cut(s) | 263 |
| MmeI | TCCRAC | 2 cut(s) | 219, 381 |
| MnlI | CCTC | 7 cut(s) | 52, 60, 241, 292, 294, 317, 402 |
| MseI | TTAA | 3 cut(s) | 104, 233, 413 |
| MslI | CAYNNNNRTG | 2 cut(s) | 83, 164 |
| MspA1I | CMGCKG | 2 cut(s) | 337, 488 |
| MspI | CCGG | 2 cut(s) | 346, 437 |
| MspR9I | CCNGG | 5 cut(s) | 319, 346, 437, 469, 474 |
| MvaI | CCWGG | 3 cut(s) | 319, 469, 474 |
| MvnI | CGCG | 2 cut(s) | 139, 464 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 134, 145 |
| NciI | CCSGG | 2 cut(s) | 346, 437 |
| NdeII | GATC | 2 cut(s) | 7, 326 |
| NheI | GCTAGC | 1 cut(s) | 199 |
| NlaIII | CATG | 1 cut(s) | 74 |
| NlaIV | GGNNCC | 4 cut(s) | 180, 213, 433, 441 |
| NmeAIII | GCCGAG | 1 cut(s) | 82 |
| NmuCI | GTSAC | 1 cut(s) | 150 |
| NspI | RCATGY | 1 cut(s) | 74 |
| OliI | CACNNNNGTG | 1 cut(s) | 83 |
| PfeI | GAWTC | 1 cut(s) | 425 |
| PflFI | GACNNNGTC | 1 cut(s) | 356 |
| PkrI | GCNGC | 2 cut(s) | 396, 490 |
| PleI | GAGTC | 1 cut(s) | 262 |
| PpsI | GAGTC | 1 cut(s) | 262 |
| PpuMI | RGGWCCY | 1 cut(s) | 178 |
| PshBI | ATTAAT | 1 cut(s) | 233 |
| Psp5II | RGGWCCY | 1 cut(s) | 178 |
| Psp6I | CCWGG | 3 cut(s) | 317, 467, 472 |
| PspFI | CCCAGC | 1 cut(s) | 133 |
| PspGI | CCWGG | 3 cut(s) | 317, 467, 472 |
| PspN4I | GGNNCC | 4 cut(s) | 180, 213, 433, 441 |
| PspPI | GGNCC | 4 cut(s) | 61, 178, 212, 476 |
| PspPPI | RGGWCCY | 1 cut(s) | 178 |
| PsuI | RGATCY | 1 cut(s) | 7 |
| PsyI | GACNNNGTC | 1 cut(s) | 356 |
| RsaI | GTAC | 1 cut(s) | 343 |
| RsaNI | GTAC | 1 cut(s) | 342 |
| RseI | CAYNNNNRTG | 2 cut(s) | 83, 164 |
| SaqAI | TTAA | 3 cut(s) | 104, 233, 413 |
| SatI | GCNGC | 2 cut(s) | 395, 489 |
| Sau3AI | GATC | 2 cut(s) | 7, 326 |
| Sau96I | GGNCC | 4 cut(s) | 61, 178, 212, 476 |
| SchI | GAGTC | 1 cut(s) | 263 |
| ScrFI | CCNGG | 5 cut(s) | 319, 346, 437, 469, 474 |
| SetI | ASST | 7 cut(s) | 34, 180, 201, 286, 376, 413, 448 |
| SfaNI | GCATC | 3 cut(s) | 178, 355, 381 |
| SinI | GGWCC | 3 cut(s) | 178, 212, 476 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 83, 164 |
| Sse9I | AATT | 3 cut(s) | 230, 414, 492 |
| SsiI | CCGC | 9 cut(s) | 5, 91, 203, 251, 274, 312, 337, 422, 486 |
| SspMI | CTAG | 2 cut(s) | 200, 350 |
| StyD4I | CCNGG | 5 cut(s) | 317, 344, 435, 467, 472 |
| TaiI | ACGT | 1 cut(s) | 34 |
| TaqI | TCGA | 1 cut(s) | 278 |
| TasI | AATT | 3 cut(s) | 230, 414, 492 |
| TfiI | GAWTC | 1 cut(s) | 425 |
| Tru1I | TTAA | 3 cut(s) | 104, 233, 413 |
| Tru9I | TTAA | 3 cut(s) | 104, 233, 413 |
| TscAI | CASTG | 3 cut(s) | 130, 189, 267 |
| TseFI | GTSAC | 1 cut(s) | 150 |
| TseI | GCWGC | 2 cut(s) | 394, 488 |
| Tsp45I | GTSAC | 1 cut(s) | 150 |
| TspRI | CASTG | 3 cut(s) | 130, 189, 267 |
| Tth111I | GACNNNGTC | 1 cut(s) | 356 |
| VpaK11BI | GGWCC | 3 cut(s) | 178, 212, 476 |
| VspI | ATTAAT | 1 cut(s) | 233 |
| XceI | RCATGY | 1 cut(s) | 74 |
| XspI | CTAG | 2 cut(s) | 200, 350 |