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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 247 |
| Acc36I | ACCTGC | 2 cut(s) | 247, 394 |
| AccBSI | CCGCTC | 1 cut(s) | 505 |
| AccII | CGCG | 1 cut(s) | 90 |
| AciI | CCGC | 9 cut(s) | 15, 67, 74, 88, 120, 191, 410, 443, 503 |
| AclWI | GGATC | 1 cut(s) | 201 |
| AcoI | YGGCCR | 1 cut(s) | 261 |
| AfaI | GTAC | 1 cut(s) | 367 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 73, 256 |
| AjnI | CCWGG | 3 cut(s) | 115, 151, 347 |
| AleI | CACNNNNGTG | 2 cut(s) | 107, 269 |
| AluBI | AGCT | 1 cut(s) | 427 |
| AluI | AGCT | 1 cut(s) | 427 |
| Alw21I | GWGCWC | 1 cut(s) | 347 |
| Alw26I | GTCTC | 1 cut(s) | 119 |
| AlwI | GGATC | 1 cut(s) | 201 |
| AoxI | GGCC | 5 cut(s) | 154, 240, 246, 261, 440 |
| ApeKI | GCWGC | 1 cut(s) | 141 |
| AspLEI | GCGC | 2 cut(s) | 92, 374 |
| AspS9I | GGNCC | 4 cut(s) | 247, 406, 415, 440 |
| AsuC2I | CCSGG | 2 cut(s) | 71, 320 |
| AsuHPI | GGTGA | 2 cut(s) | 134, 157 |
| AvaII | GGWCC | 2 cut(s) | 406, 415 |
| BaeGI | GKGCMC | 1 cut(s) | 457 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| Bbv12I | GWGCWC | 1 cut(s) | 347 |
| BbvI | GCAGC | 1 cut(s) | 153 |
| BceAI | ACGGC | 1 cut(s) | 219 |
| BciT130I | CCWGG | 3 cut(s) | 117, 153, 349 |
| BcnI | CCSGG | 2 cut(s) | 71, 320 |
| BcoDI | GTCTC | 1 cut(s) | 119 |
| BfaI | CTAG | 3 cut(s) | 188, 363, 508 |
| BfuAI | ACCTGC | 2 cut(s) | 247, 394 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 320 |
| BisI | GCNGC | 2 cut(s) | 142, 443 |
| BlsI | GCNGC | 2 cut(s) | 143, 444 |
| Bme1390I | CCNGG | 5 cut(s) | 71, 117, 153, 320, 349 |
| Bme18I | GGWCC | 2 cut(s) | 406, 415 |
| BmgT120I | GGNCC | 4 cut(s) | 247, 406, 415, 440 |
| BmiI | GGNNCC | 2 cut(s) | 408, 417 |
| BmrFI | CCNGG | 5 cut(s) | 71, 117, 153, 320, 349 |
| BmrI | ACTGGG | 2 cut(s) | 101, 452 |
| BmuI | ACTGGG | 2 cut(s) | 101, 452 |
| BpmI | CTGGAG | 1 cut(s) | 323 |
| BpuMI | CCSGG | 2 cut(s) | 71, 320 |
| BsaJI | CCNNGG | 2 cut(s) | 115, 243 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 73, 256 |
| Bse1I | ACTGG | 3 cut(s) | 107, 389, 458 |
| BseBI | CCWGG | 3 cut(s) | 117, 153, 349 |
| BseDI | CCNNGG | 2 cut(s) | 115, 243 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 73, 256 |
| BseMII | CTCAG | 1 cut(s) | 281 |
| BseNI | ACTGG | 3 cut(s) | 107, 389, 458 |
| BseSI | GKGCMC | 1 cut(s) | 457 |
| BseXI | GCAGC | 1 cut(s) | 153 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| BshFI | GGCC | 5 cut(s) | 156, 242, 248, 263, 442 |
| BsiHKAI | GWGCWC | 1 cut(s) | 347 |
| BsiSI | CCGG | 2 cut(s) | 71, 320 |
| BslFI | GGGAC | 1 cut(s) | 392 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 73, 256 |
| BsmAI | GTCTC | 1 cut(s) | 119 |
| BsmFI | GGGAC | 1 cut(s) | 392 |
| BsnI | GGCC | 5 cut(s) | 156, 242, 248, 263, 442 |
| Bsp1286I | GDGCHC | 2 cut(s) | 347, 457 |
| Bsp143I | GATC | 2 cut(s) | 40, 193 |
| BspACI | CCGC | 9 cut(s) | 15, 67, 74, 88, 120, 191, 410, 443, 503 |
| BspANI | GGCC | 5 cut(s) | 156, 242, 248, 263, 442 |
| BspCNI | CTCAG | 1 cut(s) | 282 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspLI | GGNNCC | 2 cut(s) | 408, 417 |
| BspMI | ACCTGC | 2 cut(s) | 247, 394 |
| BspPI | GGATC | 1 cut(s) | 201 |
| BsrBI | CCGCTC | 1 cut(s) | 505 |
| BsrI | ACTGG | 3 cut(s) | 107, 389, 458 |
| BssECI | CCNNGG | 2 cut(s) | 115, 243 |
| BssMI | GATC | 2 cut(s) | 40, 193 |
| Bst2UI | CCWGG | 3 cut(s) | 117, 153, 349 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 336 |
| BstDEI | CTNAG | 1 cut(s) | 290 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstHHI | GCGC | 2 cut(s) | 92, 374 |
| BstKTI | GATC | 2 cut(s) | 43, 196 |
| BstMAI | GTCTC | 1 cut(s) | 119 |
| BstMBI | GATC | 2 cut(s) | 40, 193 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 320, 409 |
| BstNI | CCWGG | 3 cut(s) | 117, 153, 349 |
| BstSCI | CCNGG | 5 cut(s) | 69, 115, 151, 318, 347 |
| BstSLI | GKGCMC | 1 cut(s) | 457 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 1 cut(s) | 153 |
| BstX2I | RGATCY | 1 cut(s) | 193 |
| BstYI | RGATCY | 1 cut(s) | 193 |
| BsuRI | GGCC | 5 cut(s) | 156, 242, 248, 263, 442 |
| BtsIMutI | CAGTG | 1 cut(s) | 114 |
| BveI | ACCTGC | 2 cut(s) | 247, 394 |
| CfoI | GCGC | 2 cut(s) | 92, 374 |
| Cfr13I | GGNCC | 4 cut(s) | 247, 406, 415, 440 |
| Csp6I | GTAC | 1 cut(s) | 366 |
| CviQI | GTAC | 1 cut(s) | 366 |
| DdeI | CTNAG | 1 cut(s) | 290 |
| DpnI | GATC | 2 cut(s) | 42, 195 |
| DpnII | GATC | 2 cut(s) | 40, 193 |
| EaeI | YGGCCR | 1 cut(s) | 261 |
| Eco47I | GGWCC | 2 cut(s) | 406, 415 |
| EcoO109I | RGGNCCY | 1 cut(s) | 406 |
| EcoRII | CCWGG | 3 cut(s) | 115, 151, 347 |
| FaiI | YATR | 4 cut(s) | 177, 201, 399, 401 |
| FaqI | GGGAC | 1 cut(s) | 392 |
| FauI | CCCGC | 3 cut(s) | 8, 74, 417 |
| FauNDI | CATATG | 1 cut(s) | 399 |
| Fnu4HI | GCNGC | 2 cut(s) | 142, 443 |
| Fsp4HI | GCNGC | 2 cut(s) | 142, 443 |
| FspBI | CTAG | 3 cut(s) | 188, 363, 508 |
| GlaI | GCGC | 2 cut(s) | 91, 373 |
| GluI | GCNGC | 2 cut(s) | 142, 443 |
| GsuI | CTGGAG | 1 cut(s) | 323 |
| HaeIII | GGCC | 5 cut(s) | 156, 242, 248, 263, 442 |
| HapII | CCGG | 2 cut(s) | 71, 320 |
| HhaI | GCGC | 2 cut(s) | 92, 374 |
| Hin6I | GCGC | 2 cut(s) | 90, 372 |
| HinP1I | GCGC | 2 cut(s) | 90, 372 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HinfI | GANTC | 1 cut(s) | 483 |
| HpaII | CCGG | 2 cut(s) | 71, 320 |
| HphI | GGTGA | 2 cut(s) | 134, 157 |
| Hpy166II | GTNNAC | 4 cut(s) | 79, 85, 112, 221 |
| Hpy188III | TCNNGA | 1 cut(s) | 233 |
| Hpy8I | GTNNAC | 4 cut(s) | 79, 85, 112, 221 |
| HpyAV | CCTTC | 1 cut(s) | 28 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 336 |
| HpyCH4IV | ACGT | 4 cut(s) | 81, 217, 266, 277 |
| HpyCH4V | TGCA | 2 cut(s) | 397, 403 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 320, 409 |
| HpyF3I | CTNAG | 1 cut(s) | 290 |
| HpySE526I | ACGT | 4 cut(s) | 81, 217, 266, 277 |
| HspAI | GCGC | 2 cut(s) | 90, 372 |
| Kzo9I | GATC | 2 cut(s) | 40, 193 |
| LmnI | GCTCC | 2 cut(s) | 327, 342 |
| Lsp1109I | GCAGC | 1 cut(s) | 153 |
| MaeI | CTAG | 3 cut(s) | 188, 363, 508 |
| MaeII | ACGT | 4 cut(s) | 81, 217, 266, 277 |
| MaeIII | GTNAC | 2 cut(s) | 273, 336 |
| MalI | GATC | 2 cut(s) | 42, 195 |
| MbiI | CCGCTC | 1 cut(s) | 505 |
| MboI | GATC | 2 cut(s) | 40, 193 |
| MboII | GAAGA | 2 cut(s) | 188, 306 |
| MflI | RGATCY | 1 cut(s) | 193 |
| MhlI | GDGCHC | 2 cut(s) | 347, 457 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MnlI | CCTC | 6 cut(s) | 155, 173, 178, 238, 298, 470 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| MseI | TTAA | 1 cut(s) | 462 |
| MslI | CAYNNNNRTG | 2 cut(s) | 107, 269 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MspA1I | CMGCKG | 1 cut(s) | 412 |
| MspI | CCGG | 2 cut(s) | 71, 320 |
| MspR9I | CCNGG | 5 cut(s) | 71, 117, 153, 320, 349 |
| MvaI | CCWGG | 3 cut(s) | 117, 153, 349 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 320, 409 |
| NciI | CCSGG | 2 cut(s) | 71, 320 |
| NdeI | CATATG | 1 cut(s) | 399 |
| NdeII | GATC | 2 cut(s) | 40, 193 |
| NlaIV | GGNNCC | 2 cut(s) | 408, 417 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 2 cut(s) | 273, 336 |
| OliI | CACNNNNGTG | 2 cut(s) | 107, 269 |
| PaqCI | CACCTGC | 1 cut(s) | 247 |
| PfeI | GAWTC | 1 cut(s) | 483 |
| PkrI | GCNGC | 2 cut(s) | 143, 444 |
| PpuMI | RGGWCCY | 1 cut(s) | 406 |
| Psp5II | RGGWCCY | 1 cut(s) | 406 |
| Psp6I | CCWGG | 3 cut(s) | 115, 151, 347 |
| PspGI | CCWGG | 3 cut(s) | 115, 151, 347 |
| PspN4I | GGNNCC | 2 cut(s) | 408, 417 |
| PspPI | GGNCC | 4 cut(s) | 247, 406, 415, 440 |
| PspPPI | RGGWCCY | 1 cut(s) | 406 |
| PsuI | RGATCY | 1 cut(s) | 193 |
| RsaI | GTAC | 1 cut(s) | 367 |
| RsaNI | GTAC | 1 cut(s) | 366 |
| RseI | CAYNNNNRTG | 2 cut(s) | 107, 269 |
| SaqAI | TTAA | 1 cut(s) | 462 |
| SatI | GCNGC | 2 cut(s) | 142, 443 |
| Sau3AI | GATC | 2 cut(s) | 40, 193 |
| Sau96I | GGNCC | 4 cut(s) | 247, 406, 415, 440 |
| ScrFI | CCNGG | 5 cut(s) | 71, 117, 153, 320, 349 |
| SduI | GDGCHC | 2 cut(s) | 347, 457 |
| SinI | GGWCC | 2 cut(s) | 406, 415 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 107, 269 |
| SsiI | CCGC | 9 cut(s) | 15, 67, 74, 88, 120, 191, 410, 443, 503 |
| SspMI | CTAG | 3 cut(s) | 188, 363, 508 |
| StyD4I | CCNGG | 5 cut(s) | 69, 115, 151, 318, 347 |
| TaaI | ACNGT | 2 cut(s) | 11, 336 |
| TaiI | ACGT | 4 cut(s) | 84, 220, 269, 280 |
| TauI | GCSGC | 1 cut(s) | 445 |
| TfiI | GAWTC | 1 cut(s) | 483 |
| Tru1I | TTAA | 1 cut(s) | 462 |
| Tru9I | TTAA | 1 cut(s) | 462 |
| TscAI | CASTG | 1 cut(s) | 114 |
| TseFI | GTSAC | 2 cut(s) | 273, 336 |
| TseI | GCWGC | 1 cut(s) | 141 |
| Tsp45I | GTSAC | 2 cut(s) | 273, 336 |
| TspDTI | ATGAA | 2 cut(s) | 188, 300 |
| TspGWI | ACGGA | 2 cut(s) | 340, 355 |
| TspRI | CASTG | 1 cut(s) | 114 |
| VpaK11BI | GGWCC | 2 cut(s) | 406, 415 |
| XspI | CTAG | 3 cut(s) | 188, 363, 508 |