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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 535 |
| AccB1I | GGYRCC | 1 cut(s) | 535 |
| AccII | CGCG | 2 cut(s) | 282, 496 |
| AciI | CCGC | 8 cut(s) | 273, 280, 282, 351, 356, 496, 528, 608 |
| AcoI | YGGCCR | 1 cut(s) | 417 |
| AcuI | CTGAAG | 1 cut(s) | 467 |
| AcyI | GRCGYC | 1 cut(s) | 296 |
| AfaI | GTAC | 2 cut(s) | 537, 564 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 223, 362, 524 |
| AgsI | TTSAA | 1 cut(s) | 132 |
| AjnI | CCWGG | 1 cut(s) | 538 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 440, 472 |
| AluBI | AGCT | 4 cut(s) | 67, 330, 445, 583 |
| AluI | AGCT | 4 cut(s) | 67, 330, 445, 583 |
| Alw21I | GWGCWC | 2 cut(s) | 332, 503 |
| Alw26I | GTCTC | 2 cut(s) | 166, 290 |
| AoxI | GGCC | 4 cut(s) | 192, 396, 402, 417 |
| ApaI | GGGCCC | 1 cut(s) | 196 |
| ApeKI | GCWGC | 2 cut(s) | 185, 243 |
| Asp718I | GGTACC | 1 cut(s) | 535 |
| AspLEI | GCGC | 3 cut(s) | 74, 387, 481 |
| AspS9I | GGNCC | 8 cut(s) | 54, 192, 193, 353, 365, 403, 542, 596 |
| AsuHPI | GGTGA | 2 cut(s) | 58, 238 |
| AvaII | GGWCC | 5 cut(s) | 54, 353, 365, 542, 596 |
| BaeGI | GKGCMC | 1 cut(s) | 196 |
| BalI | TGGCCA | 1 cut(s) | 419 |
| BanI | GGYRCC | 1 cut(s) | 535 |
| BanII | GRGCYC | 2 cut(s) | 196, 332 |
| BbsI | GAAGAC | 1 cut(s) | 275 |
| Bbv12I | GWGCWC | 2 cut(s) | 332, 503 |
| BbvI | GCAGC | 2 cut(s) | 172, 230 |
| BccI | CCATC | 1 cut(s) | 368 |
| BceAI | ACGGC | 1 cut(s) | 198 |
| BciT130I | CCWGG | 1 cut(s) | 540 |
| BcoDI | GTCTC | 2 cut(s) | 166, 290 |
| BfaI | CTAG | 1 cut(s) | 68 |
| BfoI | RGCGCY | 1 cut(s) | 75 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 252 |
| BisI | GCNGC | 2 cut(s) | 186, 244 |
| BlsI | GCNGC | 2 cut(s) | 187, 245 |
| BmcAI | AGTACT | 1 cut(s) | 564 |
| Bme1390I | CCNGG | 1 cut(s) | 540 |
| Bme18I | GGWCC | 5 cut(s) | 54, 353, 365, 542, 596 |
| BmgT120I | GGNCC | 8 cut(s) | 54, 192, 193, 353, 365, 403, 542, 596 |
| BmiI | GGNNCC | 5 cut(s) | 55, 56, 194, 537, 597 |
| BmrFI | CCNGG | 1 cut(s) | 540 |
| BmrI | ACTGGG | 1 cut(s) | 211 |
| BmsI | GCATC | 2 cut(s) | 264, 546 |
| BmuI | ACTGGG | 1 cut(s) | 211 |
| BpiI | GAAGAC | 1 cut(s) | 275 |
| BsaAI | YACGTR | 1 cut(s) | 587 |
| BsaHI | GRCGYC | 1 cut(s) | 296 |
| BsaI | GGTCTC | 1 cut(s) | 290 |
| BsaJI | CCNNGG | 2 cut(s) | 280, 399 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 223, 362, 524 |
| Bse118I | RCCGGY | 2 cut(s) | 223, 524 |
| Bse1I | ACTGG | 1 cut(s) | 217 |
| Bse3DI | GCAATG | 1 cut(s) | 27 |
| BseBI | CCWGG | 1 cut(s) | 540 |
| BseDI | CCNNGG | 2 cut(s) | 280, 399 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 223, 362, 524 |
| BseMI | GCAATG | 1 cut(s) | 27 |
| BseMII | CTCAG | 2 cut(s) | 218, 324 |
| BseNI | ACTGG | 1 cut(s) | 217 |
| BseRI | GAGGAG | 1 cut(s) | 594 |
| BseSI | GKGCMC | 1 cut(s) | 196 |
| BseXI | GCAGC | 2 cut(s) | 172, 230 |
| BseYI | CCCAGC | 1 cut(s) | 475 |
| BsgI | GTGCAG | 1 cut(s) | 171 |
| Bsh1236I | CGCG | 2 cut(s) | 282, 496 |
| BshFI | GGCC | 4 cut(s) | 194, 398, 404, 419 |
| BshNI | GGYRCC | 1 cut(s) | 535 |
| BsiHKAI | GWGCWC | 2 cut(s) | 332, 503 |
| BsiSI | CCGG | 2 cut(s) | 224, 525 |
| BslFI | GGGAC | 2 cut(s) | 67, 609 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 223, 362, 524 |
| BsmAI | GTCTC | 2 cut(s) | 166, 290 |
| BsmBI | CGTCTC | 1 cut(s) | 166 |
| BsmFI | GGGAC | 2 cut(s) | 67, 609 |
| BsnI | GGCC | 4 cut(s) | 194, 398, 404, 419 |
| Bso31I | GGTCTC | 1 cut(s) | 290 |
| Bsp120I | GGGCCC | 1 cut(s) | 192 |
| Bsp1286I | GDGCHC | 3 cut(s) | 196, 332, 503 |
| BspACI | CCGC | 8 cut(s) | 273, 280, 282, 351, 356, 496, 528, 608 |
| BspANI | GGCC | 4 cut(s) | 194, 398, 404, 419 |
| BspCNI | CTCAG | 2 cut(s) | 217, 325 |
| BspFNI | CGCG | 2 cut(s) | 282, 496 |
| BspLI | GGNNCC | 5 cut(s) | 55, 56, 194, 537, 597 |
| BspT107I | GGYRCC | 1 cut(s) | 535 |
| BspTNI | GGTCTC | 1 cut(s) | 290 |
| BsrDI | GCAATG | 1 cut(s) | 27 |
| BsrFI | RCCGGY | 2 cut(s) | 223, 524 |
| BsrI | ACTGG | 1 cut(s) | 217 |
| BssAI | RCCGGY | 2 cut(s) | 223, 524 |
| BssECI | CCNNGG | 2 cut(s) | 280, 399 |
| BssNI | GRCGYC | 1 cut(s) | 296 |
| Bst2UI | CCWGG | 1 cut(s) | 540 |
| Bst6I | CTCTTC | 1 cut(s) | 443 |
| BstACI | GRCGYC | 1 cut(s) | 296 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 565 |
| BstBAI | YACGTR | 1 cut(s) | 587 |
| BstC8I | GCNNGC | 2 cut(s) | 253, 488 |
| BstDEI | CTNAG | 2 cut(s) | 204, 333 |
| BstDSI | CCRYGG | 1 cut(s) | 280 |
| BstFNI | CGCG | 2 cut(s) | 282, 496 |
| BstH2I | RGCGCY | 1 cut(s) | 75 |
| BstHHI | GCGC | 3 cut(s) | 74, 387, 481 |
| BstMAI | GTCTC | 2 cut(s) | 166, 290 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 191, 252, 461, 476, 487, 507, 565 |
| BstNI | CCWGG | 1 cut(s) | 540 |
| BstSCI | CCNGG | 1 cut(s) | 538 |
| BstSLI | GKGCMC | 1 cut(s) | 196 |
| BstUI | CGCG | 2 cut(s) | 282, 496 |
| BstV1I | GCAGC | 2 cut(s) | 172, 230 |
| BstV2I | GAAGAC | 1 cut(s) | 275 |
| BsuRI | GGCC | 4 cut(s) | 194, 398, 404, 419 |
| BtgI | CCRYGG | 1 cut(s) | 280 |
| Cac8I | GCNNGC | 2 cut(s) | 253, 488 |
| CfoI | GCGC | 3 cut(s) | 74, 387, 481 |
| Cfr10I | RCCGGY | 2 cut(s) | 223, 524 |
| Cfr13I | GGNCC | 8 cut(s) | 54, 192, 193, 353, 365, 403, 542, 596 |
| Cfr42I | CCGCGG | 1 cut(s) | 283 |
| CpoI | CGGWCCG | 1 cut(s) | 353 |
| CseI | GACGC | 2 cut(s) | 304, 516 |
| Csp6I | GTAC | 2 cut(s) | 536, 563 |
| CspI | CGGWCCG | 1 cut(s) | 353 |
| CviQI | GTAC | 2 cut(s) | 536, 563 |
| DdeI | CTNAG | 2 cut(s) | 204, 333 |
| EaeI | YGGCCR | 1 cut(s) | 417 |
| Eam1104I | CTCTTC | 1 cut(s) | 443 |
| EarI | CTCTTC | 1 cut(s) | 443 |
| EciI | GGCGGA | 2 cut(s) | 345, 597 |
| Ecl136II | GAGCTC | 1 cut(s) | 330 |
| Eco24I | GRGCYC | 2 cut(s) | 196, 332 |
| Eco31I | GGTCTC | 1 cut(s) | 290 |
| Eco47I | GGWCC | 5 cut(s) | 54, 353, 365, 542, 596 |
| Eco53kI | GAGCTC | 1 cut(s) | 330 |
| Eco57I | CTGAAG | 1 cut(s) | 467 |
| EcoICRI | GAGCTC | 1 cut(s) | 330 |
| EcoO109I | RGGNCCY | 2 cut(s) | 54, 193 |
| EcoRII | CCWGG | 1 cut(s) | 538 |
| EcoT38I | GRGCYC | 2 cut(s) | 196, 332 |
| Esp3I | CGTCTC | 1 cut(s) | 166 |
| FaiI | YATR | 3 cut(s) | 39, 344, 551 |
| FaqI | GGGAC | 2 cut(s) | 67, 609 |
| FauI | CCCGC | 1 cut(s) | 521 |
| Fnu4HI | GCNGC | 2 cut(s) | 186, 244 |
| FriOI | GRGCYC | 2 cut(s) | 196, 332 |
| Fsp4HI | GCNGC | 2 cut(s) | 186, 244 |
| FspBI | CTAG | 1 cut(s) | 68 |
| GlaI | GCGC | 3 cut(s) | 73, 386, 480 |
| GluI | GCNGC | 2 cut(s) | 186, 244 |
| GsaI | CCCAGC | 1 cut(s) | 479 |
| HaeII | RGCGCY | 1 cut(s) | 75 |
| HaeIII | GGCC | 4 cut(s) | 194, 398, 404, 419 |
| HapII | CCGG | 2 cut(s) | 224, 525 |
| HgaI | GACGC | 2 cut(s) | 304, 516 |
| HhaI | GCGC | 3 cut(s) | 74, 387, 481 |
| Hin1I | GRCGYC | 1 cut(s) | 296 |
| Hin6I | GCGC | 3 cut(s) | 72, 385, 479 |
| HinP1I | GCGC | 3 cut(s) | 72, 385, 479 |
| HincII | GTYRAC | 1 cut(s) | 375 |
| HindII | GTYRAC | 1 cut(s) | 375 |
| HinfI | GANTC | 1 cut(s) | 107 |
| HpaI | GTTAAC | 1 cut(s) | 375 |
| HpaII | CCGG | 2 cut(s) | 224, 525 |
| HphI | GGTGA | 2 cut(s) | 58, 238 |
| Hpy166II | GTNNAC | 1 cut(s) | 375 |
| Hpy188I | TCNGA | 3 cut(s) | 260, 334, 365 |
| Hpy188III | TCNNGA | 1 cut(s) | 432 |
| Hpy8I | GTNNAC | 1 cut(s) | 375 |
| Hpy99I | CGWCG | 3 cut(s) | 155, 158, 161 |
| HpyAV | CCTTC | 2 cut(s) | 10, 43 |
| HpyCH4IV | ACGT | 2 cut(s) | 150, 586 |
| HpyCH4V | TGCA | 2 cut(s) | 188, 559 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 191, 252, 461, 476, 487, 507, 565 |
| HpyF3I | CTNAG | 2 cut(s) | 204, 333 |
| HpySE526I | ACGT | 2 cut(s) | 150, 586 |
| Hsp92I | GRCGYC | 1 cut(s) | 296 |
| HspAI | GCGC | 3 cut(s) | 72, 385, 479 |
| KflI | GGGWCCC | 1 cut(s) | 54 |
| KpnI | GGTACC | 1 cut(s) | 539 |
| KspAI | GTTAAC | 1 cut(s) | 375 |
| KspI | CCGCGG | 1 cut(s) | 283 |
| LmnI | GCTCC | 3 cut(s) | 327, 382, 498 |
| Lsp1109I | GCAGC | 2 cut(s) | 172, 230 |
| LweI | GCATC | 2 cut(s) | 264, 546 |
| MaeI | CTAG | 1 cut(s) | 68 |
| MaeII | ACGT | 2 cut(s) | 150, 586 |
| MaeIII | GTNAC | 1 cut(s) | 226 |
| MboII | GAAGA | 4 cut(s) | 116, 124, 280, 460 |
| MhlI | GDGCHC | 3 cut(s) | 196, 332, 503 |
| MlsI | TGGCCA | 1 cut(s) | 419 |
| MluNI | TGGCCA | 1 cut(s) | 419 |
| MmeI | TCCRAC | 1 cut(s) | 438 |
| MnlI | CCTC | 7 cut(s) | 206, 219, 257, 310, 394, 612, 615 |
| Mox20I | TGGCCA | 1 cut(s) | 419 |
| MscI | TGGCCA | 1 cut(s) | 419 |
| MseI | TTAA | 3 cut(s) | 80, 87, 374 |
| MslI | CAYNNNNRTG | 2 cut(s) | 425, 554 |
| Msp20I | TGGCCA | 1 cut(s) | 419 |
| MspA1I | CMGCKG | 1 cut(s) | 282 |
| MspI | CCGG | 2 cut(s) | 224, 525 |
| MspR9I | CCNGG | 1 cut(s) | 540 |
| MvaI | CCWGG | 1 cut(s) | 540 |
| MvnI | CGCG | 2 cut(s) | 282, 496 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 191, 252, 461, 476, 487, 507, 565 |
| NlaIV | GGNNCC | 5 cut(s) | 55, 56, 194, 537, 597 |
| NmeAIII | GCCGAG | 1 cut(s) | 424 |
| NmuCI | GTSAC | 1 cut(s) | 226 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 156 |
| PfeI | GAWTC | 1 cut(s) | 107 |
| PflFI | GACNNNGTC | 1 cut(s) | 510 |
| PkrI | GCNGC | 2 cut(s) | 187, 245 |
| Ppu21I | YACGTR | 1 cut(s) | 587 |
| PpuMI | RGGWCCY | 1 cut(s) | 54 |
| Psp124BI | GAGCTC | 1 cut(s) | 332 |
| Psp5II | RGGWCCY | 1 cut(s) | 54 |
| Psp6I | CCWGG | 1 cut(s) | 538 |
| PspFI | CCCAGC | 1 cut(s) | 475 |
| PspGI | CCWGG | 1 cut(s) | 538 |
| PspN4I | GGNNCC | 5 cut(s) | 55, 56, 194, 537, 597 |
| PspOMI | GGGCCC | 1 cut(s) | 192 |
| PspPI | GGNCC | 8 cut(s) | 54, 192, 193, 353, 365, 403, 542, 596 |
| PspPPI | RGGWCCY | 1 cut(s) | 54 |
| PsyI | GACNNNGTC | 1 cut(s) | 510 |
| RsaI | GTAC | 2 cut(s) | 537, 564 |
| RsaNI | GTAC | 2 cut(s) | 536, 563 |
| RseI | CAYNNNNRTG | 2 cut(s) | 425, 554 |
| Rsr2I | CGGWCCG | 1 cut(s) | 353 |
| RsrII | CGGWCCG | 1 cut(s) | 353 |
| SacI | GAGCTC | 1 cut(s) | 332 |
| SacII | CCGCGG | 1 cut(s) | 283 |
| SaqAI | TTAA | 3 cut(s) | 80, 87, 374 |
| SatI | GCNGC | 2 cut(s) | 186, 244 |
| Sau96I | GGNCC | 8 cut(s) | 54, 192, 193, 353, 365, 403, 542, 596 |
| ScaI | AGTACT | 1 cut(s) | 564 |
| ScrFI | CCNGG | 1 cut(s) | 540 |
| SduI | GDGCHC | 3 cut(s) | 196, 332, 503 |
| SetI | ASST | 9 cut(s) | 69, 153, 322, 332, 349, 447, 585, 589, 604 |
| SfaNI | GCATC | 2 cut(s) | 264, 546 |
| Sfr303I | CCGCGG | 1 cut(s) | 283 |
| SgrAI | CRCCGGYG | 2 cut(s) | 223, 524 |
| SgrBI | CCGCGG | 1 cut(s) | 283 |
| SinI | GGWCC | 5 cut(s) | 54, 353, 365, 542, 596 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 425, 554 |
| SsiI | CCGC | 8 cut(s) | 273, 280, 282, 351, 356, 496, 528, 608 |
| SspMI | CTAG | 1 cut(s) | 68 |
| SstI | GAGCTC | 1 cut(s) | 332 |
| StyD4I | CCNGG | 1 cut(s) | 538 |
| TaiI | ACGT | 2 cut(s) | 153, 589 |
| TaqI | TCGA | 1 cut(s) | 110 |
| TatI | WGTACW | 1 cut(s) | 562 |
| TfiI | GAWTC | 1 cut(s) | 107 |
| Tru1I | TTAA | 3 cut(s) | 80, 87, 374 |
| Tru9I | TTAA | 3 cut(s) | 80, 87, 374 |
| TseFI | GTSAC | 1 cut(s) | 226 |
| TseI | GCWGC | 2 cut(s) | 185, 243 |
| Tsp45I | GTSAC | 1 cut(s) | 226 |
| Tth111I | GACNNNGTC | 1 cut(s) | 510 |
| VpaK11BI | GGWCC | 5 cut(s) | 54, 353, 365, 542, 596 |
| XspI | CTAG | 1 cut(s) | 68 |
| ZrmI | AGTACT | 1 cut(s) | 564 |