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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 262 |
| AciI | CCGC | 4 cut(s) | 15, 262, 294, 332 |
| AclI | AACGTT | 1 cut(s) | 99 |
| AclWI | GGATC | 1 cut(s) | 48 |
| AcoI | YGGCCR | 1 cut(s) | 183 |
| AcsI | RAATTY | 1 cut(s) | 200 |
| AcuI | CTGAAG | 1 cut(s) | 233 |
| AfaI | GTAC | 2 cut(s) | 303, 330 |
| AgsI | TTSAA | 1 cut(s) | 90 |
| AluBI | AGCT | 3 cut(s) | 139, 211, 349 |
| AluI | AGCT | 3 cut(s) | 139, 211, 349 |
| Alw21I | GWGCWC | 3 cut(s) | 112, 141, 269 |
| Alw44I | GTGCAC | 1 cut(s) | 108 |
| AlwI | GGATC | 1 cut(s) | 48 |
| AoxI | GGCC | 2 cut(s) | 168, 183 |
| ApaLI | GTGCAC | 1 cut(s) | 108 |
| ApoI | RAATTY | 1 cut(s) | 200 |
| AspLEI | GCGC | 2 cut(s) | 153, 223 |
| AspS9I | GGNCC | 2 cut(s) | 169, 362 |
| AsuHPI | GGTGA | 1 cut(s) | 280 |
| AvaII | GGWCC | 1 cut(s) | 362 |
| BaeGI | GKGCMC | 1 cut(s) | 112 |
| BalI | TGGCCA | 1 cut(s) | 185 |
| BanII | GRGCYC | 1 cut(s) | 141 |
| Bbv12I | GWGCWC | 3 cut(s) | 112, 141, 269 |
| BccI | CCATC | 1 cut(s) | 293 |
| BfaI | CTAG | 2 cut(s) | 270, 344 |
| BfoI | RGCGCY | 1 cut(s) | 224 |
| Bme18I | GGWCC | 1 cut(s) | 362 |
| BmgT120I | GGNCC | 2 cut(s) | 169, 362 |
| BmiI | GGNNCC | 1 cut(s) | 363 |
| BmrI | ACTGGG | 1 cut(s) | 236 |
| BmsI | GCATC | 1 cut(s) | 312 |
| BmuI | ACTGGG | 1 cut(s) | 236 |
| BpuEI | CTTGAG | 1 cut(s) | 125 |
| Bse118I | RCCGGY | 1 cut(s) | 124 |
| Bse1I | ACTGG | 1 cut(s) | 242 |
| BseMII | CTCAG | 1 cut(s) | 289 |
| BseNI | ACTGG | 1 cut(s) | 242 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseSI | GKGCMC | 1 cut(s) | 112 |
| Bsh1236I | CGCG | 1 cut(s) | 262 |
| BshFI | GGCC | 2 cut(s) | 170, 185 |
| BsiHKAI | GWGCWC | 3 cut(s) | 112, 141, 269 |
| BsiSI | CCGG | 1 cut(s) | 125 |
| BslFI | GGGAC | 1 cut(s) | 375 |
| BsmFI | GGGAC | 1 cut(s) | 375 |
| BsnI | GGCC | 2 cut(s) | 170, 185 |
| Bsp1286I | GDGCHC | 3 cut(s) | 112, 141, 269 |
| Bsp143I | GATC | 2 cut(s) | 40, 75 |
| BspACI | CCGC | 4 cut(s) | 15, 262, 294, 332 |
| BspANI | GGCC | 2 cut(s) | 170, 185 |
| BspCNI | CTCAG | 1 cut(s) | 288 |
| BspFNI | CGCG | 1 cut(s) | 262 |
| BspLI | GGNNCC | 1 cut(s) | 363 |
| BspPI | GGATC | 1 cut(s) | 48 |
| BsrFI | RCCGGY | 1 cut(s) | 124 |
| BsrI | ACTGG | 1 cut(s) | 242 |
| BssAI | RCCGGY | 1 cut(s) | 124 |
| BssMI | GATC | 2 cut(s) | 40, 75 |
| Bst4CI | ACNGT | 3 cut(s) | 11, 94, 107 |
| Bst6I | CTCTTC | 1 cut(s) | 209 |
| BstC8I | GCNNGC | 1 cut(s) | 126 |
| BstDEI | CTNAG | 1 cut(s) | 275 |
| BstFNI | CGCG | 1 cut(s) | 262 |
| BstH2I | RGCGCY | 1 cut(s) | 224 |
| BstHHI | GCGC | 2 cut(s) | 153, 223 |
| BstKTI | GATC | 2 cut(s) | 43, 78 |
| BstMBI | GATC | 2 cut(s) | 40, 75 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 253, 280, 331 |
| BstSLI | GKGCMC | 1 cut(s) | 112 |
| BstUI | CGCG | 1 cut(s) | 262 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 298 |
| BsuRI | GGCC | 2 cut(s) | 170, 185 |
| BtsIMutI | CAGTG | 2 cut(s) | 188, 249 |
| Cac8I | GCNNGC | 1 cut(s) | 126 |
| CfoI | GCGC | 2 cut(s) | 153, 223 |
| Cfr10I | RCCGGY | 1 cut(s) | 124 |
| Cfr13I | GGNCC | 2 cut(s) | 169, 362 |
| Csp6I | GTAC | 2 cut(s) | 302, 329 |
| CviJI | RGCY | 7 cut(s) | 139, 170, 185, 211, 274, 283, 349 |
| CviKI_1 | RGCY | 7 cut(s) | 139, 170, 185, 211, 274, 283, 349 |
| CviQI | GTAC | 2 cut(s) | 302, 329 |
| DdeI | CTNAG | 1 cut(s) | 275 |
| DpnI | GATC | 2 cut(s) | 42, 77 |
| DpnII | GATC | 2 cut(s) | 40, 75 |
| EaeI | YGGCCR | 1 cut(s) | 183 |
| Eam1104I | CTCTTC | 1 cut(s) | 209 |
| EarI | CTCTTC | 1 cut(s) | 209 |
| Ecl136II | GAGCTC | 1 cut(s) | 139 |
| Eco24I | GRGCYC | 1 cut(s) | 141 |
| Eco47I | GGWCC | 1 cut(s) | 362 |
| Eco53kI | GAGCTC | 1 cut(s) | 139 |
| Eco57I | CTGAAG | 1 cut(s) | 233 |
| EcoICRI | GAGCTC | 1 cut(s) | 139 |
| EcoT38I | GRGCYC | 1 cut(s) | 141 |
| FaiI | YATR | 2 cut(s) | 155, 317 |
| FaqI | GGGAC | 1 cut(s) | 375 |
| FauI | CCCGC | 1 cut(s) | 8 |
| FriOI | GRGCYC | 1 cut(s) | 141 |
| FspBI | CTAG | 2 cut(s) | 270, 344 |
| GlaI | GCGC | 2 cut(s) | 152, 222 |
| HaeII | RGCGCY | 1 cut(s) | 224 |
| HaeIII | GGCC | 2 cut(s) | 170, 185 |
| HapII | CCGG | 1 cut(s) | 125 |
| HhaI | GCGC | 2 cut(s) | 153, 223 |
| Hin6I | GCGC | 2 cut(s) | 151, 221 |
| HinP1I | GCGC | 2 cut(s) | 151, 221 |
| HincII | GTYRAC | 2 cut(s) | 97, 103 |
| HindII | GTYRAC | 2 cut(s) | 97, 103 |
| HinfI | GANTC | 2 cut(s) | 130, 374 |
| HpaI | GTTAAC | 2 cut(s) | 97, 103 |
| HpaII | CCGG | 1 cut(s) | 125 |
| HphI | GGTGA | 1 cut(s) | 280 |
| Hpy166II | GTNNAC | 4 cut(s) | 97, 103, 110, 304 |
| Hpy188III | TCNNGA | 3 cut(s) | 134, 371, 378 |
| Hpy8I | GTNNAC | 4 cut(s) | 97, 103, 110, 304 |
| HpyCH4III | ACNGT | 3 cut(s) | 11, 94, 107 |
| HpyCH4IV | ACGT | 1 cut(s) | 99 |
| HpyCH4V | TGCA | 3 cut(s) | 110, 247, 325 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 253, 280, 331 |
| HpyF3I | CTNAG | 1 cut(s) | 275 |
| HpySE526I | ACGT | 1 cut(s) | 99 |
| HspAI | GCGC | 2 cut(s) | 151, 221 |
| KroI | GCCGGC | 1 cut(s) | 124 |
| KroNI | GCCGGC | 1 cut(s) | 126 |
| KspAI | GTTAAC | 2 cut(s) | 97, 103 |
| Kzo9I | GATC | 2 cut(s) | 40, 75 |
| LmnI | GCTCC | 3 cut(s) | 31, 148, 264 |
| LpnPI | CCDG | 5 cut(s) | 131, 138, 209, 255, 304 |
| LweI | GCATC | 1 cut(s) | 312 |
| MaeI | CTAG | 2 cut(s) | 270, 344 |
| MaeII | ACGT | 1 cut(s) | 99 |
| MalI | GATC | 2 cut(s) | 42, 77 |
| MboI | GATC | 2 cut(s) | 40, 75 |
| MboII | GAAGA | 2 cut(s) | 226, 239 |
| MhlI | GDGCHC | 3 cut(s) | 112, 141, 269 |
| MlsI | TGGCCA | 1 cut(s) | 185 |
| MluCI | AATT | 2 cut(s) | 85, 200 |
| MluNI | TGGCCA | 1 cut(s) | 185 |
| MnlI | CCTC | 4 cut(s) | 22, 58, 160, 210 |
| Mox20I | TGGCCA | 1 cut(s) | 185 |
| MreI | CGCCGGCG | 1 cut(s) | 124 |
| MroNI | GCCGGC | 1 cut(s) | 124 |
| MscI | TGGCCA | 1 cut(s) | 185 |
| MseI | TTAA | 4 cut(s) | 84, 96, 102, 117 |
| MslI | CAYNNNNRTG | 2 cut(s) | 191, 320 |
| Msp20I | TGGCCA | 1 cut(s) | 185 |
| MspA1I | CMGCKG | 2 cut(s) | 294, 334 |
| MspI | CCGG | 1 cut(s) | 125 |
| MvnI | CGCG | 1 cut(s) | 262 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 253, 280, 331 |
| NaeI | GCCGGC | 1 cut(s) | 126 |
| NdeII | GATC | 2 cut(s) | 40, 75 |
| NgoMIV | GCCGGC | 1 cut(s) | 124 |
| NlaIV | GGNNCC | 1 cut(s) | 363 |
| PdiI | GCCGGC | 1 cut(s) | 126 |
| PfeI | GAWTC | 2 cut(s) | 130, 374 |
| Psp124BI | GAGCTC | 1 cut(s) | 141 |
| Psp1406I | AACGTT | 1 cut(s) | 99 |
| PspN4I | GGNNCC | 1 cut(s) | 363 |
| PspPI | GGNCC | 2 cut(s) | 169, 362 |
| RsaI | GTAC | 2 cut(s) | 303, 330 |
| RsaNI | GTAC | 2 cut(s) | 302, 329 |
| RseI | CAYNNNNRTG | 2 cut(s) | 191, 320 |
| SacI | GAGCTC | 1 cut(s) | 141 |
| SaqAI | TTAA | 4 cut(s) | 84, 96, 102, 117 |
| Sau3AI | GATC | 2 cut(s) | 40, 75 |
| Sau96I | GGNCC | 2 cut(s) | 169, 362 |
| SduI | GDGCHC | 3 cut(s) | 112, 141, 269 |
| SetI | ASST | 5 cut(s) | 102, 141, 165, 213, 351 |
| SfaNI | GCATC | 1 cut(s) | 312 |
| SgrAI | CRCCGGYG | 1 cut(s) | 124 |
| SinI | GGWCC | 1 cut(s) | 362 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 191, 320 |
| SmlI | CTYRAG | 1 cut(s) | 140 |
| SmoI | CTYRAG | 1 cut(s) | 140 |
| Sse9I | AATT | 2 cut(s) | 85, 200 |
| SsiI | CCGC | 4 cut(s) | 15, 262, 294, 332 |
| SspMI | CTAG | 2 cut(s) | 270, 344 |
| SstI | GAGCTC | 1 cut(s) | 141 |
| TaaI | ACNGT | 3 cut(s) | 11, 94, 107 |
| TaiI | ACGT | 1 cut(s) | 102 |
| TaqI | TCGA | 2 cut(s) | 133, 165 |
| TasI | AATT | 2 cut(s) | 85, 200 |
| TfiI | GAWTC | 2 cut(s) | 130, 374 |
| Tru1I | TTAA | 4 cut(s) | 84, 96, 102, 117 |
| Tru9I | TTAA | 4 cut(s) | 84, 96, 102, 117 |
| TscAI | CASTG | 2 cut(s) | 195, 249 |
| TspGWI | ACGGA | 1 cut(s) | 321 |
| TspRI | CASTG | 2 cut(s) | 195, 249 |
| VneI | GTGCAC | 1 cut(s) | 108 |
| VpaK11BI | GGWCC | 1 cut(s) | 362 |
| XapI | RAATTY | 1 cut(s) | 200 |
| XspI | CTAG | 2 cut(s) | 270, 344 |