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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 274 |
| AccBSI | CCGCTC | 2 cut(s) | 165, 592 |
| AccII | CGCG | 2 cut(s) | 246, 367 |
| AclI | AACGTT | 1 cut(s) | 63 |
| AcsI | RAATTY | 1 cut(s) | 305 |
| AfaI | GTAC | 3 cut(s) | 183, 408, 435 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 178, 229, 283, 395 |
| AgsI | TTSAA | 2 cut(s) | 54, 442 |
| AjnI | CCWGG | 1 cut(s) | 111 |
| AluBI | AGCT | 4 cut(s) | 208, 454, 528, 583 |
| AluI | AGCT | 4 cut(s) | 208, 454, 528, 583 |
| Alw21I | GWGCWC | 1 cut(s) | 374 |
| Alw26I | GTCTC | 2 cut(s) | 146, 266 |
| AoxI | GGCC | 1 cut(s) | 267 |
| ApeKI | GCWGC | 2 cut(s) | 168, 528 |
| ApoI | RAATTY | 1 cut(s) | 305 |
| AseI | ATTAAT | 1 cut(s) | 48 |
| AspLEI | GCGC | 2 cut(s) | 328, 381 |
| AspS9I | GGNCC | 3 cut(s) | 4, 413, 543 |
| AsuC2I | CCSGG | 1 cut(s) | 179 |
| AvaII | GGWCC | 3 cut(s) | 4, 413, 543 |
| Bbv12I | GWGCWC | 1 cut(s) | 374 |
| BbvI | GCAGC | 2 cut(s) | 180, 515 |
| BccI | CCATC | 1 cut(s) | 401 |
| BciT130I | CCWGG | 1 cut(s) | 113 |
| BcnI | CCSGG | 1 cut(s) | 179 |
| BcoDI | GTCTC | 2 cut(s) | 146, 266 |
| BfaI | CTAG | 6 cut(s) | 117, 215, 329, 375, 573, 601 |
| BfmI | CTRYAG | 1 cut(s) | 144 |
| BfoI | RGCGCY | 1 cut(s) | 329 |
| BfuAI | ACCTGC | 1 cut(s) | 274 |
| BisI | GCNGC | 6 cut(s) | 166, 169, 172, 195, 518, 529 |
| BlsI | GCNGC | 6 cut(s) | 167, 170, 173, 196, 519, 530 |
| Bme1390I | CCNGG | 2 cut(s) | 113, 179 |
| Bme18I | GGWCC | 3 cut(s) | 4, 413, 543 |
| BmgT120I | GGNCC | 3 cut(s) | 4, 413, 543 |
| BmiI | GGNNCC | 2 cut(s) | 108, 510 |
| BmrFI | CCNGG | 2 cut(s) | 113, 179 |
| BmsI | GCATC | 1 cut(s) | 417 |
| BpuMI | CCSGG | 1 cut(s) | 179 |
| BsaI | GGTCTC | 1 cut(s) | 266 |
| BsaJI | CCNNGG | 3 cut(s) | 111, 177, 552 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 143, 173, 488, 518, 570, 600 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 178, 229, 283, 395 |
| Bse1I | ACTGG | 1 cut(s) | 89 |
| BseBI | CCWGG | 1 cut(s) | 113 |
| BseDI | CCNNGG | 3 cut(s) | 111, 177, 552 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 178, 229, 283, 395 |
| BseMII | CTCAG | 1 cut(s) | 170 |
| BseNI | ACTGG | 1 cut(s) | 89 |
| BseXI | GCAGC | 2 cut(s) | 180, 515 |
| BseYI | CCCAGC | 1 cut(s) | 98 |
| Bsh1236I | CGCG | 2 cut(s) | 246, 367 |
| BshFI | GGCC | 1 cut(s) | 269 |
| BsiHKAI | GWGCWC | 1 cut(s) | 374 |
| BsiSI | CCGG | 1 cut(s) | 179 |
| BslFI | GGGAC | 2 cut(s) | 480, 554 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 178, 229, 283, 395 |
| BsmAI | GTCTC | 2 cut(s) | 146, 266 |
| BsmFI | GGGAC | 2 cut(s) | 480, 554 |
| BsnI | GGCC | 1 cut(s) | 269 |
| Bso31I | GGTCTC | 1 cut(s) | 266 |
| Bsp1286I | GDGCHC | 1 cut(s) | 374 |
| Bsp143I | GATC | 1 cut(s) | 39 |
| BspANI | GGCC | 1 cut(s) | 269 |
| BspCNI | CTCAG | 1 cut(s) | 169 |
| BspFNI | CGCG | 2 cut(s) | 246, 367 |
| BspLI | GGNNCC | 2 cut(s) | 108, 510 |
| BspMI | ACCTGC | 1 cut(s) | 274 |
| BspTNI | GGTCTC | 1 cut(s) | 266 |
| BsrBI | CCGCTC | 2 cut(s) | 165, 592 |
| BsrI | ACTGG | 1 cut(s) | 89 |
| BssECI | CCNNGG | 3 cut(s) | 111, 177, 552 |
| BssMI | GATC | 1 cut(s) | 39 |
| BssT1I | CCWWGG | 1 cut(s) | 552 |
| Bst2UI | CCWGG | 1 cut(s) | 113 |
| Bst4CI | ACNGT | 3 cut(s) | 58, 71, 438 |
| Bst6I | CTCTTC | 1 cut(s) | 327 |
| BstC8I | GCNNGC | 3 cut(s) | 210, 248, 359 |
| BstDEI | CTNAG | 1 cut(s) | 156 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 227 |
| BstFNI | CGCG | 2 cut(s) | 246, 367 |
| BstH2I | RGCGCY | 1 cut(s) | 329 |
| BstHHI | GCGC | 2 cut(s) | 328, 381 |
| BstKTI | GATC | 1 cut(s) | 42 |
| BstMAI | GTCTC | 2 cut(s) | 146, 266 |
| BstMBI | GATC | 1 cut(s) | 39 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 171, 358, 378, 517, 589 |
| BstNI | CCWGG | 1 cut(s) | 113 |
| BstSCI | CCNGG | 2 cut(s) | 111, 177 |
| BstSFI | CTRYAG | 1 cut(s) | 144 |
| BstUI | CGCG | 2 cut(s) | 246, 367 |
| BstV1I | GCAGC | 2 cut(s) | 180, 515 |
| BsuRI | GGCC | 1 cut(s) | 269 |
| BveI | ACCTGC | 1 cut(s) | 274 |
| Cac8I | GCNNGC | 3 cut(s) | 210, 248, 359 |
| CfoI | GCGC | 2 cut(s) | 328, 381 |
| Cfr13I | GGNCC | 3 cut(s) | 4, 413, 543 |
| Csp6I | GTAC | 3 cut(s) | 182, 407, 434 |
| CviAII | CATG | 2 cut(s) | 251, 421 |
| CviQI | GTAC | 3 cut(s) | 182, 407, 434 |
| DdeI | CTNAG | 1 cut(s) | 156 |
| DpnI | GATC | 1 cut(s) | 41 |
| DpnII | GATC | 1 cut(s) | 39 |
| Eam1104I | CTCTTC | 1 cut(s) | 327 |
| EarI | CTCTTC | 1 cut(s) | 327 |
| Eco130I | CCWWGG | 1 cut(s) | 552 |
| Eco31I | GGTCTC | 1 cut(s) | 266 |
| Eco47I | GGWCC | 3 cut(s) | 4, 413, 543 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 227 |
| EcoRII | CCWGG | 1 cut(s) | 111 |
| EcoT14I | CCWWGG | 1 cut(s) | 552 |
| ErhI | CCWWGG | 1 cut(s) | 552 |
| FaeI | CATG | 2 cut(s) | 254, 424 |
| FaiI | YATR | 6 cut(s) | 200, 204, 252, 422, 522, 563 |
| FaqI | GGGAC | 2 cut(s) | 480, 554 |
| FatI | CATG | 2 cut(s) | 250, 420 |
| FauI | CCCGC | 3 cut(s) | 19, 95, 138 |
| Fnu4HI | GCNGC | 6 cut(s) | 166, 169, 172, 195, 518, 529 |
| Fsp4HI | GCNGC | 6 cut(s) | 166, 169, 172, 195, 518, 529 |
| FspBI | CTAG | 6 cut(s) | 117, 215, 329, 375, 573, 601 |
| GlaI | GCGC | 2 cut(s) | 327, 380 |
| GluI | GCNGC | 6 cut(s) | 166, 169, 172, 195, 518, 529 |
| GsaI | CCCAGC | 1 cut(s) | 102 |
| HaeII | RGCGCY | 1 cut(s) | 329 |
| HaeIII | GGCC | 1 cut(s) | 269 |
| HapII | CCGG | 1 cut(s) | 179 |
| HhaI | GCGC | 2 cut(s) | 328, 381 |
| Hin1II | CATG | 2 cut(s) | 254, 424 |
| Hin6I | GCGC | 2 cut(s) | 326, 379 |
| HinP1I | GCGC | 2 cut(s) | 326, 379 |
| HincII | GTYRAC | 3 cut(s) | 61, 67, 386 |
| HindII | GTYRAC | 3 cut(s) | 61, 67, 386 |
| HinfI | GANTC | 2 cut(s) | 154, 503 |
| HpaI | GTTAAC | 2 cut(s) | 61, 67 |
| HpaII | CCGG | 1 cut(s) | 179 |
| Hpy166II | GTNNAC | 4 cut(s) | 61, 67, 386, 409 |
| Hpy188I | TCNGA | 1 cut(s) | 508 |
| Hpy188III | TCNNGA | 2 cut(s) | 476, 541 |
| Hpy8I | GTNNAC | 4 cut(s) | 61, 67, 386, 409 |
| HpyAV | CCTTC | 2 cut(s) | 233, 316 |
| HpyCH4III | ACNGT | 3 cut(s) | 58, 71, 438 |
| HpyCH4IV | ACGT | 3 cut(s) | 63, 256, 293 |
| HpyCH4V | TGCA | 3 cut(s) | 74, 424, 430 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 171, 358, 378, 517, 589 |
| HpyF3I | CTNAG | 1 cut(s) | 156 |
| HpySE526I | ACGT | 3 cut(s) | 63, 256, 293 |
| Hsp92II | CATG | 2 cut(s) | 254, 424 |
| HspAI | GCGC | 2 cut(s) | 326, 379 |
| KspAI | GTTAAC | 2 cut(s) | 61, 67 |
| Kzo9I | GATC | 1 cut(s) | 39 |
| LmnI | GCTCC | 4 cut(s) | 369, 496, 508, 594 |
| Lsp1109I | GCAGC | 2 cut(s) | 180, 515 |
| LweI | GCATC | 1 cut(s) | 417 |
| MaeI | CTAG | 6 cut(s) | 117, 215, 329, 375, 573, 601 |
| MaeII | ACGT | 3 cut(s) | 63, 256, 293 |
| MaeIII | GTNAC | 1 cut(s) | 119 |
| MalI | GATC | 1 cut(s) | 41 |
| MbiI | CCGCTC | 2 cut(s) | 165, 592 |
| MboI | GATC | 1 cut(s) | 39 |
| MboII | GAAGA | 1 cut(s) | 344 |
| MhlI | GDGCHC | 1 cut(s) | 374 |
| MluCI | AATT | 2 cut(s) | 49, 305 |
| MlyI | GAGTC | 2 cut(s) | 148, 512 |
| MmeI | TCCRAC | 2 cut(s) | 474, 486 |
| MnlI | CCTC | 1 cut(s) | 483 |
| MseI | TTAA | 3 cut(s) | 48, 60, 66 |
| MslI | CAYNNNNRTG | 2 cut(s) | 296, 425 |
| MspA1I | CMGCKG | 2 cut(s) | 102, 528 |
| MspI | CCGG | 1 cut(s) | 179 |
| MspR9I | CCNGG | 2 cut(s) | 113, 179 |
| MvaI | CCWGG | 1 cut(s) | 113 |
| MvnI | CGCG | 2 cut(s) | 246, 367 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 171, 358, 378, 517, 589 |
| NciI | CCSGG | 1 cut(s) | 179 |
| NdeII | GATC | 1 cut(s) | 39 |
| NlaIII | CATG | 2 cut(s) | 254, 424 |
| NlaIV | GGNNCC | 2 cut(s) | 108, 510 |
| NmeAIII | GCCGAG | 1 cut(s) | 295 |
| PflFI | GACNNNGTC | 1 cut(s) | 157 |
| PkrI | GCNGC | 6 cut(s) | 167, 170, 173, 196, 519, 530 |
| PleI | GAGTC | 2 cut(s) | 148, 511 |
| PpsI | GAGTC | 2 cut(s) | 148, 511 |
| PshBI | ATTAAT | 1 cut(s) | 48 |
| Psp1406I | AACGTT | 1 cut(s) | 63 |
| Psp6I | CCWGG | 1 cut(s) | 111 |
| PspFI | CCCAGC | 1 cut(s) | 98 |
| PspGI | CCWGG | 1 cut(s) | 111 |
| PspN4I | GGNNCC | 2 cut(s) | 108, 510 |
| PspPI | GGNCC | 3 cut(s) | 4, 413, 543 |
| PsyI | GACNNNGTC | 1 cut(s) | 157 |
| PvuII | CAGCTG | 1 cut(s) | 528 |
| RsaI | GTAC | 3 cut(s) | 183, 408, 435 |
| RsaNI | GTAC | 3 cut(s) | 182, 407, 434 |
| RseI | CAYNNNNRTG | 2 cut(s) | 296, 425 |
| SaqAI | TTAA | 3 cut(s) | 48, 60, 66 |
| SatI | GCNGC | 6 cut(s) | 166, 169, 172, 195, 518, 529 |
| Sau3AI | GATC | 1 cut(s) | 39 |
| Sau96I | GGNCC | 3 cut(s) | 4, 413, 543 |
| SchI | GAGTC | 2 cut(s) | 148, 512 |
| ScrFI | CCNGG | 2 cut(s) | 113, 179 |
| SduI | GDGCHC | 1 cut(s) | 374 |
| SfaNI | GCATC | 1 cut(s) | 417 |
| SfcI | CTRYAG | 1 cut(s) | 144 |
| SinI | GGWCC | 3 cut(s) | 4, 413, 543 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 296, 425 |
| Sse9I | AATT | 2 cut(s) | 49, 305 |
| SspMI | CTAG | 6 cut(s) | 117, 215, 329, 375, 573, 601 |
| StyD4I | CCNGG | 2 cut(s) | 111, 177 |
| StyI | CCWWGG | 1 cut(s) | 552 |
| TaaI | ACNGT | 3 cut(s) | 58, 71, 438 |
| TaiI | ACGT | 3 cut(s) | 66, 259, 296 |
| TaqI | TCGA | 1 cut(s) | 161 |
| TasI | AATT | 2 cut(s) | 49, 305 |
| TauI | GCSGC | 4 cut(s) | 168, 174, 197, 520 |
| Tru1I | TTAA | 3 cut(s) | 48, 60, 66 |
| Tru9I | TTAA | 3 cut(s) | 48, 60, 66 |
| TseI | GCWGC | 2 cut(s) | 168, 528 |
| TspDTI | ATGAA | 2 cut(s) | 267, 327 |
| TspGWI | ACGGA | 1 cut(s) | 426 |
| Tth111I | GACNNNGTC | 1 cut(s) | 157 |
| VpaK11BI | GGWCC | 3 cut(s) | 4, 413, 543 |
| VspI | ATTAAT | 1 cut(s) | 48 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 227 |
| XapI | RAATTY | 1 cut(s) | 305 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 400 |
| XspI | CTAG | 6 cut(s) | 117, 215, 329, 375, 573, 601 |