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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 721 |
| Acc36I | ACCTGC | 3 cut(s) | 421, 568, 721 |
| Acc65I | GGTACC | 2 cut(s) | 553, 580 |
| AccB1I | GGYRCC | 3 cut(s) | 101, 553, 580 |
| AccBSI | CCGCTC | 1 cut(s) | 312 |
| AccII | CGCG | 2 cut(s) | 167, 393 |
| AcoI | YGGCCR | 1 cut(s) | 64 |
| AcuI | CTGAAG | 2 cut(s) | 190, 485 |
| AcyI | GRCGYC | 1 cut(s) | 102 |
| AfaI | GTAC | 3 cut(s) | 555, 582, 668 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 41, 93, 292, 325, 430, 630, 719, 750 |
| AgeI | ACCGGT | 3 cut(s) | 86, 325, 760 |
| AgsI | TTSAA | 2 cut(s) | 138, 223 |
| AjnI | CCWGG | 6 cut(s) | 120, 270, 521, 629, 718, 746 |
| AluBI | AGCT | 7 cut(s) | 204, 213, 302, 355, 463, 601, 729 |
| AluI | AGCT | 7 cut(s) | 204, 213, 302, 355, 463, 601, 729 |
| Alw21I | GWGCWC | 2 cut(s) | 19, 521 |
| Alw44I | GTGCAC | 1 cut(s) | 15 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 213, 277 |
| AoxI | GGCC | 7 cut(s) | 64, 168, 178, 420, 613, 731, 754 |
| ApaI | GGGCCC | 2 cut(s) | 617, 758 |
| ApaLI | GTGCAC | 1 cut(s) | 15 |
| ApeKI | GCWGC | 1 cut(s) | 146 |
| AsiGI | ACCGGT | 3 cut(s) | 86, 325, 760 |
| Asp718I | GGTACC | 2 cut(s) | 553, 580 |
| AspLEI | GCGC | 6 cut(s) | 104, 393, 405, 475, 499, 528 |
| AsuC2I | CCSGG | 2 cut(s) | 36, 558 |
| AsuHPI | GGTGA | 3 cut(s) | 148, 163, 775 |
| AsuII | TTCGAA | 1 cut(s) | 30 |
| AvaII | GGWCC | 3 cut(s) | 274, 334, 560 |
| BaeGI | GKGCMC | 3 cut(s) | 19, 617, 758 |
| BanI | GGYRCC | 3 cut(s) | 101, 553, 580 |
| BanII | GRGCYC | 2 cut(s) | 617, 758 |
| Bbv12I | GWGCWC | 2 cut(s) | 19, 521 |
| BbvI | GCAGC | 1 cut(s) | 158 |
| BccI | CCATC | 2 cut(s) | 439, 604 |
| BceAI | ACGGC | 2 cut(s) | 59, 510 |
| BciT130I | CCWGG | 6 cut(s) | 122, 272, 523, 631, 720, 748 |
| BclI | TGATCA | 2 cut(s) | 153, 684 |
| BcnI | CCSGG | 2 cut(s) | 36, 558 |
| BfaI | CTAG | 1 cut(s) | 693 |
| BfoI | RGCGCY | 2 cut(s) | 105, 476 |
| BfuAI | ACCTGC | 3 cut(s) | 421, 568, 721 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 494 |
| BisI | GCNGC | 6 cut(s) | 105, 147, 168, 178, 313, 342 |
| BlsI | GCNGC | 6 cut(s) | 106, 148, 169, 179, 314, 343 |
| Bme1390I | CCNGG | 8 cut(s) | 36, 122, 272, 523, 558, 631, 720, 748 |
| Bme18I | GGWCC | 3 cut(s) | 274, 334, 560 |
| BmiI | GGNNCC | 7 cut(s) | 103, 555, 582, 615, 742, 755, 756 |
| BmrFI | CCNGG | 8 cut(s) | 36, 122, 272, 523, 558, 631, 720, 748 |
| BmsI | GCATC | 1 cut(s) | 564 |
| BpmI | CTGGAG | 1 cut(s) | 730 |
| Bpu14I | TTCGAA | 1 cut(s) | 30 |
| BpuMI | CCSGG | 2 cut(s) | 36, 558 |
| BsaHI | GRCGYC | 1 cut(s) | 102 |
| BsaJI | CCNNGG | 6 cut(s) | 121, 172, 270, 417, 629, 718 |
| BsaWI | WCCGGW | 4 cut(s) | 19, 86, 325, 760 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 641, 671 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 41, 93, 292, 325, 430, 630, 719, 750 |
| Bse118I | RCCGGY | 5 cut(s) | 66, 86, 107, 325, 760 |
| Bse1I | ACTGG | 1 cut(s) | 366 |
| BseBI | CCWGG | 6 cut(s) | 122, 272, 523, 631, 720, 748 |
| BseDI | CCNNGG | 6 cut(s) | 121, 172, 270, 417, 629, 718 |
| BseGI | GGATG | 2 cut(s) | 50, 615 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 41, 93, 292, 325, 430, 630, 719, 750 |
| BseMII | CTCAG | 2 cut(s) | 317, 690 |
| BseNI | ACTGG | 1 cut(s) | 366 |
| BseSI | GKGCMC | 3 cut(s) | 19, 617, 758 |
| BseXI | GCAGC | 1 cut(s) | 158 |
| BseYI | CCCAGC | 1 cut(s) | 213 |
| BsgI | GTGCAG | 1 cut(s) | 350 |
| Bsh1236I | CGCG | 2 cut(s) | 167, 393 |
| Bsh1285I | CGRYCG | 1 cut(s) | 87 |
| BshFI | GGCC | 7 cut(s) | 66, 170, 180, 422, 615, 733, 756 |
| BshNI | GGYRCC | 3 cut(s) | 101, 553, 580 |
| BshTI | ACCGGT | 3 cut(s) | 86, 325, 760 |
| BsiEI | CGRYCG | 1 cut(s) | 87 |
| BsiHKAI | GWGCWC | 2 cut(s) | 19, 521 |
| BsiSI | CCGG | 8 cut(s) | 20, 35, 67, 87, 108, 326, 558, 761 |
| BslFI | GGGAC | 1 cut(s) | 219 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 41, 93, 292, 325, 430, 630, 719, 750 |
| BsmFI | GGGAC | 1 cut(s) | 219 |
| BsmI | GAATGC | 1 cut(s) | 528 |
| BsnI | GGCC | 7 cut(s) | 66, 170, 180, 422, 615, 733, 756 |
| Bsp119I | TTCGAA | 1 cut(s) | 30 |
| Bsp120I | GGGCCC | 2 cut(s) | 613, 754 |
| Bsp1286I | GDGCHC | 4 cut(s) | 19, 521, 617, 758 |
| Bsp143I | GATC | 3 cut(s) | 153, 257, 684 |
| BspANI | GGCC | 7 cut(s) | 66, 170, 180, 422, 615, 733, 756 |
| BspCNI | CTCAG | 2 cut(s) | 316, 691 |
| BspFNI | CGCG | 2 cut(s) | 167, 393 |
| BspLI | GGNNCC | 7 cut(s) | 103, 555, 582, 615, 742, 755, 756 |
| BspMI | ACCTGC | 3 cut(s) | 421, 568, 721 |
| BspT104I | TTCGAA | 1 cut(s) | 30 |
| BspT107I | GGYRCC | 3 cut(s) | 101, 553, 580 |
| BsrBI | CCGCTC | 1 cut(s) | 312 |
| BsrFI | RCCGGY | 5 cut(s) | 66, 86, 107, 325, 760 |
| BsrI | ACTGG | 1 cut(s) | 366 |
| BssAI | RCCGGY | 5 cut(s) | 66, 86, 107, 325, 760 |
| BssECI | CCNNGG | 6 cut(s) | 121, 172, 270, 417, 629, 718 |
| BssMI | GATC | 3 cut(s) | 153, 257, 684 |
| BssNI | GRCGYC | 1 cut(s) | 102 |
| Bst2UI | CCWGG | 6 cut(s) | 122, 272, 523, 631, 720, 748 |
| Bst4CI | ACNGT | 2 cut(s) | 295, 666 |
| BstACI | GRCGYC | 1 cut(s) | 102 |
| BstBI | TTCGAA | 1 cut(s) | 30 |
| BstC8I | GCNNGC | 4 cut(s) | 357, 395, 506, 731 |
| BstDEI | CTNAG | 2 cut(s) | 303, 699 |
| BstDSI | CCRYGG | 1 cut(s) | 172 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 717 |
| BstF5I | GGATG | 2 cut(s) | 50, 615 |
| BstFNI | CGCG | 2 cut(s) | 167, 393 |
| BstH2I | RGCGCY | 2 cut(s) | 105, 476 |
| BstHHI | GCGC | 6 cut(s) | 104, 393, 405, 475, 499, 528 |
| BstKTI | GATC | 3 cut(s) | 156, 260, 687 |
| BstMBI | GATC | 3 cut(s) | 153, 257, 684 |
| BstMCI | CGRYCG | 1 cut(s) | 87 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 167, 183, 210, 479, 494, 505, 525, 532 |
| BstNI | CCWGG | 6 cut(s) | 122, 272, 523, 631, 720, 748 |
| BstSCI | CCNGG | 8 cut(s) | 34, 120, 270, 521, 556, 629, 718, 746 |
| BstSLI | GKGCMC | 3 cut(s) | 19, 617, 758 |
| BstUI | CGCG | 2 cut(s) | 167, 393 |
| BstV1I | GCAGC | 1 cut(s) | 158 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 550 |
| BsuRI | GGCC | 7 cut(s) | 66, 170, 180, 422, 615, 733, 756 |
| BtgI | CCRYGG | 1 cut(s) | 172 |
| BtsCI | GGATG | 2 cut(s) | 50, 615 |
| BtsIMutI | CAGTG | 2 cut(s) | 359, 584 |
| BveI | ACCTGC | 3 cut(s) | 421, 568, 721 |
| Cac8I | GCNNGC | 4 cut(s) | 357, 395, 506, 731 |
| CaiI | CAGNNNCTG | 2 cut(s) | 213, 277 |
| CfoI | GCGC | 6 cut(s) | 104, 393, 405, 475, 499, 528 |
| Cfr10I | RCCGGY | 5 cut(s) | 66, 86, 107, 325, 760 |
| CseI | GACGC | 2 cut(s) | 173, 775 |
| Csp6I | GTAC | 3 cut(s) | 554, 581, 667 |
| CspAI | ACCGGT | 3 cut(s) | 86, 325, 760 |
| CviAII | CATG | 2 cut(s) | 249, 439 |
| CviQI | GTAC | 3 cut(s) | 554, 581, 667 |
| DdeI | CTNAG | 2 cut(s) | 303, 699 |
| DinI | GGCGCC | 1 cut(s) | 103 |
| DpnI | GATC | 3 cut(s) | 155, 259, 686 |
| DpnII | GATC | 3 cut(s) | 153, 257, 684 |
| EaeI | YGGCCR | 1 cut(s) | 64 |
| EciI | GGCGGA | 1 cut(s) | 668 |
| Eco24I | GRGCYC | 2 cut(s) | 617, 758 |
| Eco47I | GGWCC | 3 cut(s) | 274, 334, 560 |
| Eco57I | CTGAAG | 2 cut(s) | 190, 485 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 717 |
| EcoO109I | RGGNCCY | 3 cut(s) | 274, 334, 754 |
| EcoRII | CCWGG | 6 cut(s) | 120, 270, 521, 629, 718, 746 |
| EcoT38I | GRGCYC | 2 cut(s) | 617, 758 |
| EgeI | GGCGCC | 1 cut(s) | 103 |
| EheI | GGCGCC | 1 cut(s) | 103 |
| FaeI | CATG | 2 cut(s) | 252, 442 |
| FaiI | YATR | 8 cut(s) | 114, 142, 144, 250, 347, 351, 440, 569 |
| FaqI | GGGAC | 1 cut(s) | 219 |
| FatI | CATG | 2 cut(s) | 248, 438 |
| FauI | CCCGC | 1 cut(s) | 744 |
| FbaI | TGATCA | 2 cut(s) | 153, 684 |
| Fnu4HI | GCNGC | 6 cut(s) | 105, 147, 168, 178, 313, 342 |
| FokI | GGATG | 2 cut(s) | 57, 622 |
| FriOI | GRGCYC | 2 cut(s) | 617, 758 |
| Fsp4HI | GCNGC | 6 cut(s) | 105, 147, 168, 178, 313, 342 |
| FspBI | CTAG | 1 cut(s) | 693 |
| GlaI | GCGC | 6 cut(s) | 103, 392, 404, 474, 498, 527 |
| GluI | GCNGC | 6 cut(s) | 105, 147, 168, 178, 313, 342 |
| GsaI | CCCAGC | 1 cut(s) | 217 |
| GsuI | CTGGAG | 1 cut(s) | 730 |
| HaeII | RGCGCY | 2 cut(s) | 105, 476 |
| HaeIII | GGCC | 7 cut(s) | 66, 170, 180, 422, 615, 733, 756 |
| HapII | CCGG | 8 cut(s) | 20, 35, 67, 87, 108, 326, 558, 761 |
| HgaI | GACGC | 2 cut(s) | 173, 775 |
| HhaI | GCGC | 6 cut(s) | 104, 393, 405, 475, 499, 528 |
| Hin1I | GRCGYC | 1 cut(s) | 102 |
| Hin1II | CATG | 2 cut(s) | 252, 442 |
| Hin6I | GCGC | 6 cut(s) | 102, 391, 403, 473, 497, 526 |
| HinP1I | GCGC | 6 cut(s) | 102, 391, 403, 473, 497, 526 |
| HindIII | AAGCTT | 1 cut(s) | 202 |
| HinfI | GANTC | 2 cut(s) | 117, 656 |
| HpaII | CCGG | 8 cut(s) | 20, 35, 67, 87, 108, 326, 558, 761 |
| HphI | GGTGA | 3 cut(s) | 148, 163, 775 |
| Hpy166II | GTNNAC | 2 cut(s) | 17, 669 |
| Hpy188I | TCNGA | 3 cut(s) | 84, 200, 209 |
| Hpy8I | GTNNAC | 2 cut(s) | 17, 669 |
| HpyAV | CCTTC | 1 cut(s) | 463 |
| HpyCH4III | ACNGT | 2 cut(s) | 295, 666 |
| HpyCH4V | TGCA | 6 cut(s) | 17, 146, 280, 331, 514, 577 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 167, 183, 210, 479, 494, 505, 525, 532 |
| HpyF3I | CTNAG | 2 cut(s) | 303, 699 |
| Hsp92I | GRCGYC | 1 cut(s) | 102 |
| Hsp92II | CATG | 2 cut(s) | 252, 442 |
| HspAI | GCGC | 6 cut(s) | 102, 391, 403, 473, 497, 526 |
| KasI | GGCGCC | 1 cut(s) | 101 |
| KpnI | GGTACC | 2 cut(s) | 557, 584 |
| Ksp22I | TGATCA | 2 cut(s) | 153, 684 |
| Kzo9I | GATC | 3 cut(s) | 153, 257, 684 |
| LmnI | GCTCC | 2 cut(s) | 388, 400 |
| Lsp1109I | GCAGC | 1 cut(s) | 158 |
| LweI | GCATC | 1 cut(s) | 564 |
| MaeI | CTAG | 1 cut(s) | 693 |
| MaeIII | GTNAC | 1 cut(s) | 763 |
| MalI | GATC | 3 cut(s) | 155, 259, 686 |
| MbiI | CCGCTC | 1 cut(s) | 312 |
| MboI | GATC | 3 cut(s) | 153, 257, 684 |
| MboII | GAAGA | 5 cut(s) | 42, 45, 48, 267, 478 |
| MfeI | CAATTG | 1 cut(s) | 509 |
| MhlI | GDGCHC | 4 cut(s) | 19, 521, 617, 758 |
| MluCI | AATT | 1 cut(s) | 509 |
| Mly113I | GGCGCC | 1 cut(s) | 102 |
| MlyI | GAGTC | 2 cut(s) | 111, 665 |
| MmeI | TCCRAC | 1 cut(s) | 107 |
| MnlI | CCTC | 8 cut(s) | 192, 329, 412, 636, 643, 694, 731, 754 |
| MseI | TTAA | 1 cut(s) | 264 |
| MslI | CAYNNNNRTG | 3 cut(s) | 22, 443, 572 |
| MspA1I | CMGCKG | 3 cut(s) | 149, 161, 546 |
| MspI | CCGG | 8 cut(s) | 20, 35, 67, 87, 108, 326, 558, 761 |
| MspR9I | CCNGG | 8 cut(s) | 36, 122, 272, 523, 558, 631, 720, 748 |
| MunI | CAATTG | 1 cut(s) | 509 |
| Mva1269I | GAATGC | 1 cut(s) | 528 |
| MvaI | CCWGG | 6 cut(s) | 122, 272, 523, 631, 720, 748 |
| MvnI | CGCG | 2 cut(s) | 167, 393 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 167, 183, 210, 479, 494, 505, 525, 532 |
| NarI | GGCGCC | 1 cut(s) | 102 |
| NciI | CCSGG | 2 cut(s) | 36, 558 |
| NdeII | GATC | 3 cut(s) | 153, 257, 684 |
| NlaIII | CATG | 2 cut(s) | 252, 442 |
| NlaIV | GGNNCC | 7 cut(s) | 103, 555, 582, 615, 742, 755, 756 |
| NmeAIII | GCCGAG | 1 cut(s) | 442 |
| NmuCI | GTSAC | 1 cut(s) | 763 |
| NspV | TTCGAA | 1 cut(s) | 30 |
| PaqCI | CACCTGC | 1 cut(s) | 721 |
| PctI | GAATGC | 1 cut(s) | 528 |
| PinAI | ACCGGT | 3 cut(s) | 86, 325, 760 |
| PkrI | GCNGC | 6 cut(s) | 106, 148, 169, 179, 314, 343 |
| PleI | GAGTC | 2 cut(s) | 111, 664 |
| PluTI | GGCGCC | 1 cut(s) | 105 |
| PpsI | GAGTC | 2 cut(s) | 111, 664 |
| PpuMI | RGGWCCY | 2 cut(s) | 274, 334 |
| Psp5II | RGGWCCY | 2 cut(s) | 274, 334 |
| Psp6I | CCWGG | 6 cut(s) | 120, 270, 521, 629, 718, 746 |
| PspFI | CCCAGC | 1 cut(s) | 213 |
| PspGI | CCWGG | 6 cut(s) | 120, 270, 521, 629, 718, 746 |
| PspN4I | GGNNCC | 7 cut(s) | 103, 555, 582, 615, 742, 755, 756 |
| PspOMI | GGGCCC | 2 cut(s) | 613, 754 |
| PspPPI | RGGWCCY | 2 cut(s) | 274, 334 |
| PstNI | CAGNNNCTG | 2 cut(s) | 213, 277 |
| RsaI | GTAC | 3 cut(s) | 555, 582, 668 |
| RsaNI | GTAC | 3 cut(s) | 554, 581, 667 |
| RseI | CAYNNNNRTG | 3 cut(s) | 22, 443, 572 |
| SaqAI | TTAA | 1 cut(s) | 264 |
| SatI | GCNGC | 6 cut(s) | 105, 147, 168, 178, 313, 342 |
| Sau3AI | GATC | 3 cut(s) | 153, 257, 684 |
| SchI | GAGTC | 2 cut(s) | 111, 665 |
| ScrFI | CCNGG | 8 cut(s) | 36, 122, 272, 523, 558, 631, 720, 748 |
| SduI | GDGCHC | 4 cut(s) | 19, 521, 617, 758 |
| SfaNI | GCATC | 1 cut(s) | 564 |
| SfoI | GGCGCC | 1 cut(s) | 103 |
| SfuI | TTCGAA | 1 cut(s) | 30 |
| SgrAI | CRCCGGYG | 2 cut(s) | 325, 760 |
| SinI | GGWCC | 3 cut(s) | 274, 334, 560 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 22, 443, 572 |
| Sse9I | AATT | 1 cut(s) | 509 |
| SspDI | GGCGCC | 1 cut(s) | 101 |
| SspI | AATATT | 1 cut(s) | 639 |
| SspMI | CTAG | 1 cut(s) | 693 |
| StyD4I | CCNGG | 8 cut(s) | 34, 120, 270, 521, 556, 629, 718, 746 |
| TaaI | ACNGT | 2 cut(s) | 295, 666 |
| TaqI | TCGA | 2 cut(s) | 30, 242 |
| TasI | AATT | 1 cut(s) | 509 |
| TauI | GCSGC | 5 cut(s) | 107, 170, 180, 315, 344 |
| Tru1I | TTAA | 1 cut(s) | 264 |
| Tru9I | TTAA | 1 cut(s) | 264 |
| TscAI | CASTG | 2 cut(s) | 366, 591 |
| TseFI | GTSAC | 1 cut(s) | 763 |
| TseI | GCWGC | 1 cut(s) | 146 |
| Tsp45I | GTSAC | 1 cut(s) | 763 |
| TspRI | CASTG | 2 cut(s) | 366, 591 |
| VneI | GTGCAC | 1 cut(s) | 15 |
| VpaK11BI | GGWCC | 3 cut(s) | 274, 334, 560 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 717 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 726 |
| XspI | CTAG | 1 cut(s) | 693 |